魯迅《自題小像》詩生成考

ArticleHumanities_Social 4: 107-127(2011)departmental bulletin pape

Selective Catalytic Hydrogenation of Cinnamaldehyde to Hydrocinnamaldehyde

采用煤质活性炭作载体,分别用HNO3、H2O2和(NH4)2S2O8进行预处理,然后用浸渍法负载PdCl2,在H2流中还原得到Pd/C催化剂,用于肉桂醛(CAL)选择加氢制氢化肉桂醛(HCAL)的反应。考查了Pd负载量,反应温度和压力对加氢反应的影响,优化了反应条件。进一步考查了助剂Fe、Co、Ni[1]对反应的影响。最终,CAL转化率为98%,HCAL选择性为88%。Coal-based activated carbon was pretreated with HNO_3,H_2O_2 and(NH_4)_2S_2O_8 before selected as support.A series of Pd/C catalysts were prepared by means of impregnation and with PdCl_2 as precursor.It was reduced in H_2 stream,and applied to selective hydrogenation of cinnamaldehyde to hydrocinnamaldehyde.Effects of Pd-loading,reaction temperature and pressure were discussed.In addition,promoters of Fe,Co,Ni were introduced to the catalysts.At last proper conditions for the reaction were found,and 88% selectivity to HCAL at 98% conversion of CAL was obtained.国家自然科学基金(No.20203014

圣·约翰草提取物片治疗抑郁障碍专家共识

结合循证医学证据,本文归纳圣·约翰草提取物片治疗抑郁障碍的专家共识

Elemene reversed the multidurg resistance of A549 /DDP lung cancer cells via mitochondrial apoptosis pathway

目的:探讨榄香烯乳(ElEMEnE,ElE)逆转人肺腺癌耐顺铂(CISPlATIn,ddP)细胞A549/ddP的耐药性及作用机制。方法:采用MTT法检测榄香烯乳单用的细胞毒作用及与ddP合用时耐药逆转作用。荧光探针JC-1结合激光共聚焦显微镜检测线粒体膜电位的变化。dCfH-dA荧光探针结合流式细胞仪检测细胞内活性氧(rEACTIVE OXygEn SPECIES,rOS)水平。用谷胱甘肽试剂盒结合分光光度法检测计算gSH/(gSSg+gSH)比值。蛋白质印迹法检测胞质中CyTO C、PrO-CASPASE-3、CASPASE-3和bCl-2家族蛋白表达情况。结果:不同浓度榄香烯乳抑制A549/ddP细胞株生长,呈时间-剂量依赖性效应,联合顺铂能提高A549/ddP细胞株对顺铂的敏感性而逆转耐药。不同浓度榄香烯乳联合顺铂使A549/ddP细胞株线粒体膜电位下降,rOS浓度增加,gSH/(gSSg+gSH)比值降低,上调胞质中CyTO C、CASPASE-3、bAd蛋白表达,下调PrO-CASPASE-3、bCl-2蛋白表达。结论:榄香烯乳逆转A549/ddP细胞株耐药性可能与其损伤线粒体膜,活化胞内氧化还原体系,诱导线粒体凋亡路径有关。Objective: To explore the mechanism that elemene( ELE) reversed the multidurg resistance( MDR)of A549 /DDP lung adenocarcinoma cell.Methods: MTT assay was used to determine the growth inhibition of human lung adenocarcinoma A549 /DDP cells in vitro.Mitochondrial membrane potential( MMP) was monitored by JC- 1fluorescence probe with laser confocal scanning microscopy,the intercellular reactive oxygen species( ROS) level was measured by 2',7'- dichlorfluorescein- diacetate( DCFH- DA) staining and flow cytometry and the ratio of GSH /( GSSG +GSH) was calculated according to detection results of GSH kit.The expression of Cytochrome C,Caspase-3 and the Bcl- 2 family proteins and in the case of cyclosporine A and DEVD- CHO,the expression of Caspase- 3expression were measured by Western blot.Results: MTT results showed that different concentrations ELE could inhibit the proliferation of A549 /DDP cells in a time- and dose- dependent manner.Intriguingly,ELE plus cisplatin enhanced the sensitivity of A549 /DDP cells to cisplatin and reversed A549 /DDP cells dury resistance.Different concentrations ELE decreased mitochondrial membrane potential,increased intracellular ROS concentration and decreased GSH /( GSSG + GSH) ratio of A549 /DDP cells in a time- and dose- dependent manner.Furthermore,the combination with both ELE and cisplatin also enhanced the protein expression of Cytoplasmic C,Caspase- 3 and Bad,and reduced the protein levels of Bcl- 2 and Pro- caspase- 3 in the cisplatin- resistant A549 /DDP cancer cells.Conclusion: ELE reversed the MDR of A549 /DDP cell line may demage mitochondrial membrane,active intracellular redox system and induce the mitochondrial apopotosis pathway.福建省自然科学基金面上项目(编号:2010D014

Studies of the Mg-Al and Cation-Incorporated Hydrotalcites

研究了掺杂阳离子水滑石的制备及阳离子对水滑石性质的影响.分别制备了掺杂Zn2+、Ni2+、Fe3+型的水滑石,考察了掺杂阳离子对水滑石晶相结构、层间距、层间阴离子含量、水滑石表面形态、水滑石碱性的影响,结果表明,由于阳离子的引入,导致水滑石层间距减小及层间阴离子结合量降低,且使水滑石培烧产物电负性提高,并最终降低其碱性.以上述水滑石培烧产物作为固体碱催化剂促进苯甲醛和丙醛缩合,合成α-甲基肉桂醛,关联了水滑石培烧产物碱性与反应活性的关系.Mg-Al and cation-incorporated(containing Zn2+?Ni2+?Fe3+) hydrotalcites were synthesized and characterized. A detailed comparative analysis of structure and composition was made. Different cations influenced strongly textural parameters of hydrotalcites and the basicity of the obtained Mg-Al oxides after calcinations. With the cation incorporation, the distance of the brucite and the mount of CO2-3 as the compensating anion changed. Meanwhile the CO2-TPD was used to determine the basicity of the samples. The results showed that the basicity of the calcined hydrotalcites reduced. Finally, the activated hydrotalcite catalysts may efficiently replace homogeneous catalysts in Claisen-Schmidt condensation reaction of the benzaldehyde and propanal. And it was noteworthy that the calcined cation-type(containing Zn2+?Fe3+) hydrotalcites as catalysts did not show significant activity, in agreement with their low basicity.国家自然科学基金(20203014)资助项

Y型聚乙二醇干扰素琢-2b注射液治疗HCV基因2/3型慢性丙型肝炎患者疗效和安全性的多中心随机对照试验研究

目的以标准剂量的聚乙二醇干扰素(Peg IFN)α-2a联合利巴韦林作为阳性对照,评价新型试验药物Y型Peg IFNα-2b注射液联合利巴韦林治疗2型/3型慢性丙型肝炎(CHC)患者的疗效和安全性。方法采用多中心、随机开放、阳性药对照的Ⅲ期临床试验,筛选符合要求的2型/3型CHC患者,按照2:1的比例随机分配到Y型Peg IFNα-2b组和Peg IFNα-2a组,同时口服利巴韦林,疗程24 w,停药随访24 w。采用Abbott Real Time HCV Genotype II检测HCV基因型,采用Cobas Taq Man实时定量PCR法检测血清HCV RNA水平。详细记录不良事件。主要疗效指标为持续病毒学应答(SVR),并进行非劣效检验。结果本试验实际入组2型/3型CHC患者255例,实际治疗241例。全分析集(FAS)数据显示,158例试验组和83例对照组患者SVR分别为85.4%(95%CI 79.94%~90.94%)和79.5%(95%CI 70.84%~88.20%,P=0.2402);对符合方案分析集(PPS)人群分析显示,试验组和对照组患者SVR分别为87.9%(95%CI 82.45%~93.27%)和85.9%(95%CI 77.82%~94.01%,P=0.7060),率差的95%可置信区间均符合非劣效标准;对PPS人群分析显示,85.8%受试者获得了早期病毒学应答(RVR),RVR的阳性预测值为90.1%;试验组和对照组不良事件发生率相似,分别为95.6%和95.2%,严重不良事件发生率分别为3.8%和3.6%。结论应用Peg IFNα联合利巴韦林治疗2型/3型CHC患者,新型试验药物Y型Peg IFNα-2b具有与对照药物Peg IFNα-2a相似的疗效和安全性。国家科技部“十二五”重大专项(编号:2012ZX10002-003)；“重大新药创制”十二五科技重大专项(编号:2012ZX09303019)

Studies of the Mg-Al and cation-incorporated hydrotalcites

Mg-Al and cation-incorporated(containing Zn2+Ni2+Fe3+) hydrotalcites were synthesized and characterized. A detailed comparative analysis of structure and composition was made. Different cations influenced strongly textural parameters of hydrotalcites and the basicity of the obtained Mg-Al oxides after calcinations. With the cation incorporation, the distance of the brucite and the mount of CO32- as the compensating anion changed. Meanwhile the CO2-TPD was used to determine the basicity of the samples. The results showed that the basicity of the calcined hydrotalcites reduced. Finally, the activated hydrotalcite catalysts may efficiently replace homogeneous catalysts in Claisen-Schmidt condensation reaction of the benzaldehyde and propanal. And it was noteworthy that the calcined cation-type (containing Zn2+, Fe3+) hydrotalcites as catalysts did not show significant activity, in agreement with their low basicity