Purification and characterization of mannase and xylanase from two Streptomyces isolated from mangrove

β-甘露聚糖酶是一类能够水解含有β-1，4-D-甘露糖苷键的甘露寡糖、甘露多糖的水解内酯酶，木聚糖酶是一类能够特异降解木聚糖的酶类。目前甘露聚糖酶和木聚糖酶己被广泛应用于食品、医药、饲料、造纸、印染、纺织等领域。链霉菌S6-204和Zxy19是从红树林土壤中筛选到的，分别具有较好甘露聚糖酶活性和木聚糖酶活性的的链霉菌。本文对链霉菌S6-204中甘露聚糖酶和链霉菌Zxy19中木聚糖酶进行了分离纯化并对纯酶组分的酶学性质进行了初步研究。 链霉菌S6-204发酵液离心去菌体，上清用硫酸铵盐析沉淀，重溶透析超滤浓缩，经DEAE-纤维素柱离子交换层析后，再分别经sephadexG75凝胶层析和Q-Se...β-mannase is an enzyme that can hydrolyze β-1,4-D-manno-pyanosyl linkage of mannan oligosaccharide and mannan,xylanase is a kind of enzyme that is capable of hydrolyzing xylan。Now, mannase and xylanase have been apply in food industry , medicine industry, feed industry, paper industry, printing and dyeing industry, spin industry and other industry . Streptomycete S6-204 and Streptomycete Zxy19 wer...学位：理学硕士院系专业：生命科学学院生物学系_微生物学学号：2162007115191

铝合金压铸高效水基脱模剂的研制

采用市售的普通原料,通过研究各种乳化剂的合理配比,调配出高效复合乳化剂对二甲基硅油和聚乙烯蜡进行乳化,制得性质稳定、脱模效果良好的铝合金水基脱模剂。其各项性能指标接近或达到了进口同类型脱模剂的水平,而价格仅为进口同类产品的40%～50%

Experimental study of using GSPS for in situ osteogenesis induction on rabbit skull

目的:研究明胶-硅氧烷多孔支架材料混合多种材料用以原位诱导兔颅骨再生的效果。方法:采用可降解、具有双孔径的明胶-硅氧烷多孔支架材料,并复合bMP2和兔骨髓间充质干细胞,在去骨瓣减压手术时将材料置于骨窗上,同时采用空白对照来探讨兔颅骨原位诱导再生。用高速螺旋CT扫描颅骨并三维重建来测量颅骨缺损的愈合情况,并作出比较。结果:支架材料+bMP组动物的颅骨修复率与支架材料+红骨髓组动物的修复率相当(P=0.43);支架材料+红骨髓组动物的颅骨修复率高于单纯支架材料组动物的修复率(P=0.01);单纯支架材料组动物的颅骨修复率与空白对照组动物的修复率无明显差别(P=0.18)。结论:明胶-硅氧烷-CA(nO3)2多孔复合支架材料分别与bMP以及红骨髓混合使用能够明显提高颅骨缺损的骨愈合速度以及修复率;而单纯使用支架材料并不能促进实验动物的颅骨修复。Objective:To investigate the effect of using GSPS for in situ osteogenesis induction on rabbit skull.Methods:The biodegradable,with double-aperture gelatin -siloxane porous scaffold materials was mixed with BMP2 or rabbit bone marrow mesenchymal stem cells to fix the skull bone deficit after craniectomy decompression.A blank control was set to compare with the effect of in situ osteogenesis of rabbit skull.High-speed spiral CT scanning and three-dimensional reconstruction of the skull was performed to inspect the healing progress of skull defects.Results:The GSPS + BMP group had the equal bone healing rate as the GSPS+ bone marrow group (P=0.43); and the GSPS + bone marrow group had the higher bone healing rate than the GSPS group (P =0.01).There were no significant difference on bone healing rate between GSPS group and blank control group (P=0.18).Conclusion:Gelatin-siloxane-Ca (NO3) 2 porous scaffolds could be used with the BMP,as well as red bone marrow to improve the bone healing of skull bone defect while no significant effect could be observed using GSPS solely

A Novel Path to Patterning Based on the Static Breath Figure Technique

李磊:2001年7月-2003年6月，在北京大学化学与分子工程学院高分子系从事博士后研究。[中文文摘]对呼吸图技术制备二维有序多孔结构的研究进展进行了综合评述,并重点介绍了本课题组发展的静态呼吸图技术.利用静态呼吸图法,可制备高度有序的聚合物、聚合物/无机物微孔膜.这些有序的结构可以直接应用于光掩膜.进一步,多孔聚合物膜可以被紫外光交联和改性.表面改性的多孔聚合物膜可以用于细胞支架.而交联的聚合物/无机物前驱体微孔膜可以用来制备无机纳米材料阵列.结果表明,静态呼吸图技术是一种简单、高效的对聚合物、聚合物/无机物薄膜进行图案化的通用方法,并展示了图案化薄膜广阔的功能化前景.[英文文摘]The breath figure technique is one of the most promising strategies for the fabrication of large-sized patterns containing an ordered two-dimensional array of holes.In this review,particular emphasis is placed on the static breath figure technique,which is a robust methodology suitable for various polymers including linear polystyrene (PS),amphiphilic diblock copolymers and polymer/inorganic precursor hybrid films.The resultant highly ordered microporous polymer films can be used as a mask for lithography.Moreover, the microporous polymer films can be surface modified and cross-linked by UV irradiation. The surface modified films can potentially be applied as a cell scaffold and the cross-linked polymer/hybrid films can be used as templates for the growth of nanomaterial arrays.国家自然科学基金(50703032,20974089); 福建省杰出青年基金(2009J06029);教育部新世纪人才基金项目资助

超临界流体选择溶胀法制备含PDMS嵌段的聚合物纳米多孔薄膜

在含亲二氧化碳链段聚二甲基硅氧烷(PDMS)的嵌段聚合物中,用超临界二氧化碳选择溶胀的方法得到了闭合的纳米孔。采用反应离子刻蚀,可以将聚合物膜内部的多孔结构暴露出来,然后用原子力显微镜和扫描电镜表征。结果表明,纳米孔的形成不是简单的PDMS纳米微区溶胀,而是由相邻纳米微区在超临界流体处理过程中合并以后形成的。纳米孔的密度和孔径分别为7.0×1010/cm2和20nm

Constraint of a ruthenium-carbon triple bond to a five-membered ring

含过渡金属碳三键(M≡C)的金属卡拜化合物是许多有机反应的催化剂或关键中间体。对其合成及性质的研究是金属有机化学的热点之一。由于卡拜碳的sp杂化方式，大部分金属卡拜化合物均为链状结构（卡拜碳键角理想值为180 °），环内金属卡拜化合物因存在很大的环张力而难于合成。夏海平教授课题组发展了由链状多炔（称之为碳龙）构筑碳龙配合物的高效方法（Nature Communications, 2017, 8, 1912），实现了锇杂戊搭炔/烯及其衍生物的一锅法合成。现在，他们把该方法进一步拓展到了第二过渡系金属钌，通过碳龙与市售的RuCl2(PPh3)3反应一锅合成了钌杂戊搭炔I。本研究是碳龙化学的进一步延伸和发展，首次把碳龙化学的金属中心由锇拓展到了其它金属，展示了碳龙化学广阔的发展空间。该研究工作在夏海平教授指导下完成，能源材料化学协同创新中心（iChEM）博士后卓庆德和张弘副教授为共同第一作者。iChEM博士后周小茜、博士生陈志昕、林剑锋、卓凯玥、硕士康慧君、林鑫磊参与了部分实验工作。博士生华煜晖负责理论计算。【Abstract】The incorporation of a metal-carbon triple bond into a ring system is challenging because of the linear nature of triple bonds. To date, the synthesis of these complexes has been limited to those containing third-row transition metal centers, namely, osmium and rhenium. We report the synthesis and full characterization of the first cyclic metal carbyne complex with a second-row transition metal center, ruthenapentalyne. It shows a bond angle of 130.2(3)° around the sp-hybridized carbyne carbon, which represents the recorded smallest angle of second-row transition metal carbyne complexes, as it deviates nearly 50° from the original angle (180°). Density functional theory calculations suggest that the inherent aromatic nature of these metallacycles with bent Ru≡C–C moieties enhances their stability. Reactivity studies showed striking observations, such as ambiphilic reactivity, a metal-carbon triple bond shift, and a [2 + 2] cycloaddition reaction with alkyne and cascade cyclization reactions with ambident nucleophiles.This research was supported by the National Key R&D Program of China (2017YFA0204902) and the National Natural Science Foundation of China (nos. 21490573,21332002, and 21561162001). 研究工作得到国家自然科学基金项目（21490573、21332002 和21561162001）和国家重点研发计划（2017YFA0204902）的资助

Honeycomb-Patterned Hybrid Films and Their Template Applications via A Tunable Amphiphilic Block Polymer/Inorganic Precursor System

通讯作者地址: Li,L(通讯作者),Xiamen Univ,Coll Mat, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Mat, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 3. Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 电子邮件地址: [email protected], we show a facile and versatile method-prepare highly ordered inorganic patterns on solid substrates by pyrolyzing UV cross-linked polymr/functional. precursor hybrid films. The crosslinked polymer matrix acted as structure-directing agent in a pyrolyzing process, whereas the functional precursor was converted into the skeleton of the micropatterns. The inorganic micropatterns could be further catalytically functionalized to grow CNT and ZnO nanorod arrays by simply changing different functional precursors. This simple technique offers new prospects in the field of micropatterns, nanolithography, and template.National Natural Science Foundation of China,50703032,20974089;Natural Science Foundation of Fujian Province,2009J06029; Ministry of Education of Chin

Patterned Carbon Nanotubes with Adjustable Array: A Functional Breath Figure Approach

通讯作者地址: Xie, SY (通讯作者), Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Mat, Xiamen 361005, Peoples R China 3. Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 电子邮件地址: [email protected], [email protected] is of paramount importance in many areas of modern science and technology. As a valuable part in miniaturized devices, large-scale aligned CNTs with serial port configuration is highly desirable. Here, we reported that the synthesis of a pair of patterned carbon nanotubes with the shape of serial port by CVD starting from different breath figure templates, cross-linked polymer matrix and ferrous inorganic micropatterns. The growth mechanism of the isolated CNT bundles is attributed to the selectively interfacial aggregation of the ferrocene to the walls of the cavities, a so-called Pickering-emulsion effect, whereas the honeycomblike skeleton of the dense CNT arrays develop from the catalytically functionalized hexagonal edges. This synthesis strategy exemplifies a new possibility for making use of CNTs to fabricate functional carbons with unique geometry or specific properties in a controllable way. We expect that the methodology can be also applied onto the fabrication of patterned graphene.National Natural Science Foundation of China 50703032,20974089 Natural Science Foundation of Fujian Province 2009J06029 Ministry of Education of Chin