Development of Analytical Method of Neomycin Residue in Water Samples and Its Application on the Analysis of Animal Products

新霉素（Neomycin）是一类抗菌活力强、作用范围广，用于治疗畜禽类疾病的氨基糖苷类抗生素。新霉素的广泛应用引发了动物食品内日益严重的残留问题，因此，新霉素的残留分析和控制显得愈发重要。本论文以阴离子交换色谱为分离方法，结合脉冲安培检测法分析水中新霉素残留，并在此基础上发展生物样品的预处理方法，将该方法成功运用于牛奶和血清样品中新霉素残留的灵敏、快速检测。研究的主要内容和结果如下： 1)水样中新霉素分析方法的建立 运用阴离子交换色谱和脉冲安培检测器，建立简单、快速、可靠的新霉素残留分析方法。考察流动相浓度和pH对色谱分离和检测的影响，确定色谱分离的最佳流动相条件为10mmol/LNaOH...Neomycin is a broad spectrum, efficiently bactericidal aminoglycoside antibiotic, which is widely used in the treatment of bacterial infections and animal diseases. The use of neomycin on a large scale as a therapeutic agent gives rise to drug residues in edible products of animals, which can adversely affect the public health and environmental safety. Therefore, residue analysis plays an importan...学位：理学硕士院系专业：海洋与环境学院环境科学与工程系_环境科学学号：20043406

Determination of 1-Hydroxyethylidene-1,1-Diphosphonic Acid in Recycle-Cooling Water by Ion Chromatography

建立了循环冷却水中羟基-1,1-亚乙基二磷酸(HEDP)的离子色谱检测方法。色谱柱为IonPac AS14A阴离子交换柱,流动相为80mmol/L NaOH水溶液,流速1.0mL/min,抑制型电导检测器检测。方法的线性范围为0.25-25mg/L,平均回收率为102%,检出限为0.1mg/L;对0.25mg/L和0.5mg/L的HEDP标准溶液分别进行13次平行测定,相对标准偏差(RSD)分别为4.7%和3.5%。该方法具有简单、快速、灵敏、抗干扰等优点,用于循环冷却水中HEDP的检测,结果令人满意。1-Hydroxyethylidene-1,1-diphosphonic acid(HEDP) is usually used as a scale and corrosion inhibitor in recycle-cooling water for steel industry.The quick and accurate analysis of HEDP concentration in water is of importance to control the inhibitor adding program.However,the common method for HEDP analysis,which determines the total phosphate in water samples,may suffer the serious problem of incorrectly including degraded HEDP as the active HEDP reagent.In this study,the method of the determination of HEDP in recycle-cooling water by ion chromatography was investigated.The chromatographic parameters,including elution and detection,were optimized.The separation was achieved on an IonPac AS14A column with NaOH solution of 80 mmol/L as the eluent at a flow rate of 1.0 mL/min,and the detection was performed by a suppressed conductivity detection mode with the injection volume of 25 μL.The method showed good linearity within the range of 0.25 and 25 mg/L with an average recovery of 102%.The detection limit of the method reached as low as 0.1 mg/L.The relative standard deviations for 0.25 and 0.5 mg/L HEDP standard solutions were 4.7% and 3.5%(n=13),respectively.The method has been applied for the determination of HEDP in recycle-cooling water samples with the advantages of being simple,fast,sensitive,and interference free.上海市科委科研计划项目课题(04-b-02

Determination of neomycin residue in milk by solid-phase extraction and ion-exchange chromatography

建立了阴离子交换色谱-脉冲安培检测分析牛奶中新霉素残留量的方法。实验采用三氯乙酸沉淀蛋白,弱阳离子交换固相萃取柱富集净化,处理牛奶样品,并考察了三氯乙酸浓度、样品过柱速度以及洗脱剂浓度等因素对牛奶中新霉素测定结果的影响。牛奶中新霉素的回收率在50.3%~76.9%范围内,基底加标工作曲线的相关系数为0.9949(n=6),线性范围为10.0~160.0ng/mL。所建立的方法可满足牛奶中低新霉素残留量的检测需要。A reliable and sensitive procedure was presented for the analysis of neomycin in milk.The method was based on a separation by ion chromatography with pulse amperometric detection.Milk was defatted and deproteinated with trichloroacetic acid,and then cleaned up with a weak cation-exchange cartridge.The influence of various parameters on clean-up of neomycin in milk,including the concentration of trichloroacetic acid,the flow rate and the concentration of the eluent,were investigated.The average recoveries of neomycin ranged from 50.3% to 76.9%.The linear calibration curve ranged from 10.0 to 160.0 ng/mL for neomycin in milk with correlation coefficient of 0.9949.The proposed method is suitable for analysis of low-level neomycin residue in milk.福建省重大科技(2003Y010)项目资

冰桶挑战邻接矩阵数据集

2014年始于美国的“冰桶挑战（Ice-Bucket Challenge）”曾经风靡世界，并在中国一度引起流行。本案例以360综合搜索（www.haosou.com）的数据为基础，结合各种中文媒体有关“冰桶挑战”的报道，采集整理实证数据并构建了“冰桶挑战”在中国社会网络传播扩散的网络数据集。该案例适用于新闻与传播专业硕士的《数据分析方法》和《社会网络的传播问题研究》等课程，对社会网络分析方法尤其具有针对性。该案例的目的是让学生通过运行网络分析软件，对“冰桶挑战”作为网络宣传的扩散路径进行分析和可视化呈现

Comparative Research on Removing Organophosphorus Pesticide Residues From Cucumber With Different Soak Water

目的比较清水、淘米水、淡盐水和洗洁精水溶液浸洗2～15min对黄瓜中有机磷农药甲胺磷和乐果的去除效果。方法采用超声波萃取和气相色谱-火焰光度检测法测定黄瓜中的有机磷农药残留。结果清水、淘米水、淡盐水和洗洁精水溶液浸泡2～15min后,黄瓜中甲胺磷和乐果的平均去除率分别为66%～22.4%和67.7%～79.6%。4种洗涤方法均可降低蔬菜瓜果中有机磷农药残留量,但不可能完全避免农药残留的危害,清水浸泡2～5min对去除黄瓜中甲胺磷和乐果残留的最高去除率接近70%。洗涤2、5、10、15min后,甲胺磷和乐果去除率变化无规律。结论清水、淘米水、淡盐水和洗洁精水溶液浸洗2～15min仍未能彻底去除黄瓜中的有机磷农药残留,随浸洗时间的延长,甲胺磷和乐果残留的去除效果无明显变化。建议采用清水浸泡2～5min的方法清洗蔬菜瓜果。Objective To evaluate the efficiency of different wash methods for removing organophosphorus pesticide residues from cucumber. Methods The cucumber samples were soaked with tap water, washed rice water, salt solution and detergent solution respectively for 2 to 15 minutes then organophosphorus pesticide residues in the soak water samples were determined with ultrasonic extraction and gas chromatography. Results Soaked in tap water for 2 to 5 minutes was the most effective and convenient way to remove methamidophos and dimethoate with the highest removing rate (about 70%). Conclusion Soaked with tap water for 2 to 5 minutes is more efficient for removing organophosphorus pesticide residues from vegetable

Effects of Nitrogen Nutrition on Photosynthesis of Dryland Crop

氮素营养与碳素同化作用间的相互关系是农学和生态学共同关注的研究热点之一。随着研究手段的改进，近年来对这一问题的研究有了较大的进展。结合自己的研究结果，就氮素营养对旱地作物碳素同化的各个生理过程的调控作用进行了简要评述

河龙区间坝库参数与减沙效益关系研究

对黄河中游河龙区间晋西北片、陕北片坝库控制面积占比、坝库单位面积库容等参数与水利水土保持措施减沙效益关系进行了研究,结果表明:(1)晋西北片若没有坝库工程,仅靠水土保持坡面治理措施只能取得14%的减沙效益;每增加100 km2的坝库控制面积,即可提高2%的减沙效益;每增加400万m3的库容,即可提高1%的减沙效益;坝库单位面积库容每提高1万m3/km2,减沙效益即可提高约10%。(2)陕北片减沙效益主要由坝库工程产生。减沙效益提高10%,坝库单位面积库容需要提高5万m3/km2。要使流域水土保持综合治理减沙效益达到20%以上,坝库单位面积库容应在6万m3/km2以上。(3)在黄河中游多沙粗沙区,要实现40%左右的减沙效益,坝库单位面积库容应达到16万m3/km2以上

Interactive Effects of Water and Fertilizer on Yield of Winter Wheat in Dryland

增施肥料可明显改善旱作农田冬小麦叶片水分状况、增加光合速率、延缓叶片衰老，有利于小麦后期维持一定的光合面积和作用时间，有利于籽粒灌浆和增加每穗粒数，也减小了土壤水分不足对产量的影响。在旱作农业中，水肥具有明显的耦合关系，肥料的增产作用不仅在于肥料本身，更重要的还在于与土壤水分的互