建立了阴离子交换色谱-脉冲安培检测分析牛奶中新霉素残留量的方法。实验采用三氯乙酸沉淀蛋白,弱阳离子交换固相萃取柱富集净化,处理牛奶样品,并考察了三氯乙酸浓度、样品过柱速度以及洗脱剂浓度等因素对牛奶中新霉素测定结果的影响。牛奶中新霉素的回收率在50.3%~76.9%范围内,基底加标工作曲线的相关系数为0.9949(n=6),线性范围为10.0~160.0ng/mL。所建立的方法可满足牛奶中低新霉素残留量的检测需要。A reliable and sensitive procedure was presented for the analysis of neomycin in milk.The method was based on a separation by ion chromatography with pulse amperometric detection.Milk was defatted and deproteinated with trichloroacetic acid,and then cleaned up with a weak cation-exchange cartridge.The influence of various parameters on clean-up of neomycin in milk,including the concentration of trichloroacetic acid,the flow rate and the concentration of the eluent,were investigated.The average recoveries of neomycin ranged from 50.3% to 76.9%.The linear calibration curve ranged from 10.0 to 160.0 ng/mL for neomycin in milk with correlation coefficient of 0.9949.The proposed method is suitable for analysis of low-level neomycin residue in milk.福建省重大科技(2003Y010)项目资
