Cloning and expression patterns of stem cells induction-associated pluripotency factors in large yellow croaker Larimichthys crocea

通过iPSCs技术获得iPSCs，建立个体特异的多能干细胞系，可为生长发育、种质资源保护、疾病防治及药物筛选研究等提供宝贵的实验材料，是一条新的从根本上解决大黄鱼养殖中的各种难题的有效策略模式。其中，多能性因子对iPSCs技术的研究是必不可少的，它们在调控胚胎干细胞（ESCs）的自我更新和多能性、原始生殖细胞（PGCs）的形成、早期胚胎发育、生殖细胞发生、器官发生、再生及增殖、生长、分化、凋亡等细胞过程等方面发挥重要作用。然而关于大黄鱼多能性因子的研究尚属空白。本文采用SMART-RACE技术克隆了大黄鱼Oct4、Sox2、Klf4和cMyc四个多能性基因，并进行了生物信息学分析；采用实时荧光...Establishment of individual specific pluripotent cell lines by iPSCs obtained through iPSCs technology is very important for the study of growth and development, germplasm resources protection, disease prevention and drug screening, etc, which can provide valuable experimental materials for these researches. Thus, it is a new effective strategy pattern for radically resolving the various problems ...学位：理学博士院系专业：海洋与地球学院_海洋生物学学号：2242010015359

Ultrastructure on oogenesis of Litopenaeus vannamei

利用透射电镜研究凡纳滨对虾卵子发生过程中细胞内部结构的变化。结果显示,卵原细胞结构简单,代谢水平低,核孔稀少,通过核周池来完成核、质之间的物质交换。卵黄发生前晚期和卵黄发生初期的卵母细胞变化显著:核膜凹凸不平,核仁数量多,核孔密集,大量核仁外排物经核孔输送到卵质中;卵质中胞器极为丰富和发达,代谢活性极强。卵黄发生旺盛期是卵黄大量形成的阶段,卵质边缘还呈辐射状排列了一圈椭圆形皮质棒,细胞出现微吞饮活动并形成卵黄膜。卵黄发生晚期卵质中充满了粗大的卵黄粒和脂滴,胞器锐减。另外,探讨了卵细胞内部结构的变化和卵黄形成的关系以及皮质棒的来源与功能。The changes of the structure in oocytes during the oogenesis of Litopenaeus vannamei were studied with transmission electron microscope. The results showed that the structure was simpler and the active metabolism is lower in oogonium.The particles from the nucleus mainly passed through the perinuclear cisternae into the cytoplasm because of fewer nuclearpores. The marked changes occurred in the latter previtellogenic oocytes and the primary vitellogenic oocytes.The nuclear changes consisted of the concavo-convex nuclear membrane,many more nucleoli, numerous nuclearpores and a large amount of nucleolus granular materials penetrating nuclear membrane into cytoplasm. There were various well-developed organelles in the cytoplasm which exhibited higher synthetic activity.The yolk granules formed mainly in active vitellogenesis. At this stage,a lap spherical cortical rods appeared radializedly at the edge of the cytoplasm.Meanwhile active microphagocytosis could be seen along the ooplasma membrane while the vitellin membrane formed on the surface of vitellogenic oocytes.The mature oocytes were full of big yolk granules and lipid droplets and the number of organelles reduced quickly. The relation between the changes of the structure in oocytes and the vitellogenesis was discussed. The origin and function of the cortical rods were also discussed.福建省教育厅自然科学基金资助项目(K02091

A sphereovirus resulted in mass mortality of Haliotis diversicolor aquatilis

对 1999年初春福建省东山县工厂化养殖九孔鲍中发生的流行性死亡 ,开展了九孔鲍的病理学研究 ,电镜下发现病鲍的肝脏细胞质中有大量具被膜 2 0面体、大小约为 10 0nm的病毒粒子。通过感染实验 ,推断该病毒是造成此次九孔鲍大量死亡的病原体 ;由于肝脏细胞的大量病变和坏死引起九孔鲍的大量死亡。该病毒的种类和分类有待进一步研究Mass mortality of abalone Haliotis diversicolor aquatilis, occurred at abalone farms of Dongshan Prefecture, Fujian Province during the spring of 1999 and Since then pathological study was performed. Electron micrography revealed mass enveloped, about 100nm in diameter, hexagonal particles in the cytoplasm of liver of diseased abalone. Artificial infected experement revealed that the virus is the lethal pathogen of mass mortality, and histopathological changes and necrosis of the liver cell were the reshan of death.A further study on specib clasifieation of virus will be needed.福建省科学委员会资助! (98-Z -10 2 ) ;; 福建省基金项目! (C96 0 33

类泛素蛋白及其中文命名

泛素家族包括泛素及类泛素蛋白,约20种成员蛋白.近年来,泛素家族领域取得了迅猛发展,并已与生物学及医学研究的各个领域相互交叉.泛素家族介导的蛋白质降解和细胞自噬机制的发现分别于2004和2016年获得诺贝尔奖.但是,类泛素蛋白并没有统一规范的中文译名. 2018年4月9日在苏州召开的《泛素家族介导的蛋白质降解和细胞自噬》专著的编委会上,部分作者讨论了类泛素蛋白的中文命名问题,并在随后的\"泛素家族、自噬与疾病\"(Ubiquitinfamily,autophagy anddiseases)苏州会议上提出了类泛素蛋白中文翻译草案,此草案在参加该会议的国内学者及海外华人学者间取得了高度共识.冷泉港亚洲\"泛素家族、自噬与疾病\"苏州会议是由美国冷泉港实验室主办、两年一度、面向全球的英文会议.该会议在海内外华人学者中具有广泛影响,因此,参会华人学者的意见具有一定的代表性.本文介绍了10个类别的类泛素蛋白的中文命名,系统总结了它们的结构特点,并比较了参与各种类泛素化修饰的酶和它们的生物学功能.文章由45名从事该领域研究的专家合作撰写,其中包括中国工程院院士1名,相关学者4名,长江学者3名,国家杰出青年科学基金获得者18名和美国知名高校华人教授4名.他们绝大多数是参加编写即将由科学出版社出版的专著《泛素家族介导的蛋白质降解和细胞自噬》的专家

Uniform Estimate of Solutions with Weight Factors for -Equations over a Strictly Pseudoconvex Domain

文[1]得到Cn空间中具有逐块C(1)光滑边界的强拟凸域上(0,q)形式的带权因子的Leray-Norguet公式的拓广式及-方程带权因子的连续解。在此基础上,利用文[2]的方法,得到了具有逐块光滑边界的强拟凸域上的-方程带权因子解的一致估计。In [1],an extensional formula of Leray-Norguet with weight factors of differential forms and weighted continuous solutions of the -equation on a strictly pseudoconvex domain with piecewise C(1) smooth boundaries in C n were obtained.On the base of [1],by using the trick of [2],the authors give the uniform estimate of weighted solutions of the -equations on a strictly pseudoconvex domain with piecewise smooth boundaries in Cn

study on scalable parallel eigenproblem solver on smp cluster

基于对称三对角特征问题的分而治之方法,提出了一个适合SMP集群环境的多级混合并行算法.SMP节点内的并行求解采用了粗粒度和细粒度两种OpenMP并行.为了改善纯MPI算法中的负载不平衡,混合并行算法使用了动态任务分配方法.在深腾6800上的试验表明,混合并行算法具有好的扩展性和加速比

长江三峡库区气候变化影响评估报告

本书是由中国气象局气候中心组织20余位在长江三峡库区研究中具有气候变化及影响研究丰富理论和实践经验的专家，经过大量资料收集、总结归纳以及作者现有研究成果撰写而成。位于长江上游的三峡库区，因其特殊的自然地理位置，深入探索气候变化对其各方面的影响，有助于积极适应和减缓气候变化，保证三峡工程安全运行，对保障三峡库区的可持续发展有重要意义。全书共分八章，在阐述三峡库区气候变化事实的基础上，分析气候变化对库区水资源、农业、自然生态系统、人体健康、电力、航运、旅游等方面的影响、脆弱性和适应性，并因地制宜地提出适应与减缓对策，为全球气候变化背景下三峡库区社会经济的可持续发展提供理论依据和科技支撑。本书是我国关于流域气候变化研究系列评估报告中的一本。本书可供中央部委和流域机构以及地方政府决策参考，亦可作为气候气象、水文水资源、生态与环境、社会经济等领域的科研人员和有关大专院校师生的参考书目

Effect of benzo(a)pyrene exposure on the testis maturity of male cuvier

本实验采用雄性褐菖鲉作为实验对象,腹腔注射0.5×10-6、1×10-6、5×10-6、10×10-6(m/m)苯并[a]芘,7d后苯并[a]芘处理组精巢睾酮水平和睾酮与17β-雌二醇的比值都有上升,但都未表现出显著性差异(P>0.05).腹腔注射50×10-6(m/m)苯并[a]芘,11d后取出精巢,常规石蜡切片,结果显示雄性褐菖鲉的精巢,精小叶腔和输出管中精子密集程度明显增加,次级精母细胞减少,精巢壁厚度变薄.结果提示,苯并[a]芘对褐菖鲉具有类雄激素效应.In this experiment,intraperitoneal injections of benzo(a)pyrene(BaP) [0.5×10~(-6),1×10~(-6),5×10~(-6),10×10~(-6)(m/m)] were given to male cuviers.7 days after the injections,levels of testosterone and testosterone/17β-estradiol in exposure groups increased,but there are no significant difference(P>0.05) between the exposure groups and the control.An intraperitoneal injection of BaP [50×10~(-6)(m/m)] was given to cuvier.11 days after the injection,testis samples were collected,dealed with common tissue section.It was observed that the density of sperm in lobular lumen and vas efferens was increased distinctly.The secondary spermatogonium was decreased.The testis wall in TBT-treated group became thiner than the control.The result indicated BaP has an androgen effect on cuvier.国家自然科学基金资助项目(40476049);; 近海海洋环境科学国家重点实验室(厦门大学)基金资