Research on Optimization of Stabilizing Fin and Longitudinal Motion Control of SWATH

SWATH船是一种具有优良耐波性的新型高性能船舶。与常规的单体船相比，它具有自然运动周期长，运动幅度小，波浪中失速少，砰击、上浪及乘员的晕船概率低等优点。由于其水线面积较小，船的纵倾恢复力矩相应减少，随着速度的增加，作用在下潜体上的MUNK力矩会以速度平方的速度迅速增加，容易发生纵向失稳。目前，解决这一问题的主要方式是在下潜体内侧安装稳定鳍。因此，稳定鳍尺寸和安装位置的确定就成为SWATH船建造过程中的一个重要环节。 定鳍虽然是平衡水动力纵倾力矩保证SWATH船纵向运动稳定的一个重要工具，但是它也对SWATH船的机动性、波浪中的运动性能以及阻力特性等方面产生较大的影响。为了获得一组较好的稳定...The small-Waterplane-area-twin-hull (SWATH) is a new kind of high-performance ship with excellent seaworthiness. Compared with the general single hull, its natural period is longer, motion amplitude is smaller, velocity lose in waves is little, probability of slams, deck wetness and nausea is lower. Because of the smaller waterplane area, the restoring moment of the longitudinal motion of a SWATH ...学位：工学硕士院系专业：信息科学与技术学院_控制理论与控制工程学号：2322010115323

Cloning and Expression of the Leukotoxin Gene SH from Fusobacterium necrophorum

坏死梭杆菌白细胞毒素是坏死杆菌病的主要致病因子,白细胞毒素基因(lkT)是其编码基因。以分离到的国内牛源坏死梭杆菌fn(A)菌株f4基因组dnA为模板,应用PCr方法扩增白细胞毒素基因SH片段,克隆至PMd18-T载体上,以bAMHⅠ和HIndⅢ酶切的目的片段SH与相应酶切的PET32A载体连接构建PET32A-SH重组表达质粒,经转化E COlI bl21(dE3)后用IPTg进行蛋白诱导,SdS-PAgE检测重组蛋白表达情况。结果表明:扩增基因序列大小为1800bP,SdS-PAgE检测重组蛋白有效表达,表达得到大小为80.2kdA的目的蛋白,采用镍柱亲和层析方法纯化SH重组蛋白,获得了纯度达95%的重组蛋白;经WEST-Ern-blOT证实,该蛋白对抗坏死杆菌阳性血清具有反应活性。The leukotoxin of Fusobacterium necrophorum(FN) is considered to be one of the main virulence factors.The lkt gene encodes for FN.In this study,the SH fragment of lkt gene was amplified by PCR using the F4 genome as the template,which was isolated from the Chinese Fusobacterium necrophorum strain.The fragment was then cloned to the pMD18-T vector for sequencing.Thereafter,the SH fragment was subcloned into the multiple cloning sites of the pET32 to construct pET32a-SH recombinant plasmid,which was then trans-formed into E.coli BL21(DE3) with IPTG induction for expression.SDS-PAGE was used to analyze the recombinant protein.The results showed that the SH fragment of about 1800 bp was amplified and was about 80.2 kDa.The fusion protein was purified by Ni-NTA affinity chromatography under denature conditions,and their purity was above 95%.Western-blot analysis indicated the SH fragment had anti-genicity against Fusobacterium necrophorum.“十五”国家科技攻关子课题(2002BA518A04);吉林省科技发展计划项目(20070570);吉林市科技发展计划项目(200805

食管癌组织中CuZn-SOD活性和表达的研究

[目的]通过对食管癌组织和癌旁组织中CuZn-SOD活性和表达检测,探讨CuZn-SOD活性和表达在食管癌发生、发展中的作用。[方法]CuZn-SOD活性和表达的检测分别采用DTNB直接法和Western Bloting方法。[结果]总SOD和CuZn-SOD的活性在食管癌组织和相应癌旁组织中的差异尚不能认为有统计学意义。30%食管癌组织中CuZn-SOD的表达明显高于癌旁组织。[结论]不同病例中表达水平结果不一致,CuZn-SOD的表达水平与肿瘤的发生或发展存在一定的联系

Research on the Longitudinal Motion Control of SWATH Based on Active Disturbance Rejection Control

针对具有非线性、强耦合性及参数不确定性的小水线面双体船(SMAll WATEr-PlAnE ArEA TWIn Hull,SWATH)纵向运动多变量控制系统,采用自抗扰控制(ACTIVE dISTurbAnCE rEJECTIOn COnTrOl,AdrC)多变量解耦控制方法设计鲁棒镇定控制器.AdrC主要特点是实时估计对象模型摄动和外扰的总和作用量,并在控制回路中加以补偿,实现对象的实时动态反馈线性化.建立了某型号SWATH纵向运动AdrC多变量解耦控制系统,并与线性二次型调节器控制效果进行比较.数值仿真表明,该控制器对模型的不确定性和外部扰动具有较好的鲁棒性和适应能力.A robust stabilization controller based on the active disturbance rejection control(ADRC)technique for a specific small water-plane area twin hull(SWATH)is presented to deal with the high nonlinearity,strong coupling and parametric uncertainties.The ADRC has the feature of estimating the combined action of the perturbation and external disturbance in real time so as to compensate for it in the control loop.Additionally,the multi-variable decoupling based on ADRC will represent the nonlinear model of a plant as a linear one,which is convenient to control for engineers.In order to show the effectiveness of the ADRC-based controller proposed in this paper,a comparison with the controller from linear quadratic regulator theory is made in simulation.Simulation results show that the designed controller has good robustness and adaptability under modeling uncertainties and external disturbances.海军总装部预研课题(XDHT2012223A

Configuration Design and Parameter Model for Flexible

设计了一种弹性喷嘴的变量喷头,其喷嘴直径随压力的增大而变大,从而降低喷头的雾化程度。为研究确定弹性喷嘴锥形角、喷嘴直径、末端圆柱段长度、弹性材料厚度和喷嘴伸出量等主要参数,利用ANSYS建立了变量喷头及其内流道的三维实体模型,采用k-&epsilon;模型在CFX和ANSYS单向流固耦合中模拟喷头弹性喷嘴在不同工作压力,不同喷嘴直径组合下的内流道流场和喷嘴的变形过程,建立了工作压力、喷嘴直径变形和喷头流量等参数的关系模型。采用全面喷洒的方法对喷头样品进行了试验,通过测量喷头流量和喷头工作压力等,对模拟结果进行验证。</span

洁净冶炼用SiAlON耐火材料的制备技术进展

碳热还原氮化工艺具有低成本且有产业化生产的潜力等特点,因而被认为是制备洁净冶炼用SiAlON耐火材料的实用方法。本文对碳热还原氮化法制备SiAlON的进展进行了综述,归纳分析了不同条件对生成物相和性能的影响。并对今后的研究进行了展望

Isolation and Identification of a High-yield Xylitol-producing Yeast Strain and Its Two-stage Fermentation Performances

通信作者: [email protected];cyao@ xmu.edu.cn[中文文摘]从300多种土壤样品中进行筛选,最终得到10株可耐受300 g/L木糖的酵母菌株,且其木糖醇转化率超过64.0%.其中酵母菌株SB18在150 g/L木糖下的木糖醇转化率为68.5%,为相同条件下转化率最大的菌株,并经18SrDNA测序鉴定为热带假丝酵母.酶活测定结果显示SB18木糖还原酶主要依赖辅因子还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH),其酶活性远大于木糖醇脱氢酶活性.对SB18进行两级法发酵条件研究,确定补充氮源为尿素和酵母提取物,接种量为0.5 g/L,转速转换时间为36 h.在此条件下由250 g/L木糖摇瓶发酵252 h得到207.65 g/L木糖醇,转化率为83.1%,达到理论值的91.8%.本研究表明酵母菌SB18在高浓度木糖醇的工业生产中具有很大的应用潜力.[英文文摘]Xylose-utilizing yeasts isolated from soil samples in Singapore were screened for xylitol production in high xylose concentration.After several round of screening,10 strains were selected for their high tolerance to 300 g/L xylose concentration,which can convert xylose into xylitol with a yield greater than 64.0%.Strain SB18 exhibited the maximal xylitol yield of 68.5% from 150 g/L xylose,and then was identified as Candida tropicalis by 18S rDNA sequencing information.Enzyme assay results indicate that in s...st rain SB18, xylose reductase(XR) is NADPH dependent and its activity is much higher than that of xylitol dehydr ogenase (XDH) . A two-stage ferment ation process was employed to investigate the cult ivation condition of SB18. The results show that the best nitro gen so urces ar e yeast extract and urea, innoculum size is 0.5 g/L, initial xylose concentration is 250 g/L, the optimal speed switch time is 36 h with a shaking speed of 250r/ min in the first stage, and a speed o f 100 r/ min afterw ards. Under the optimal cultivation conditions, xylitol final concentration reached 207. 65 g/L after 252 h incubation from 250 g/L, corresponding to a xylitol yield of 83.1% and 91.8% x ylose- to- x ylitol bio conversio n efficiency. This work demo nstrates that strain C. tropicalis SB18 has great potentials in industrial xylitol bioproduction in high xylose concentration

内陆干旱区最严格水资源管理关键技术体系研究/Key technologies for the most stringent water resources management in inland dry areas[J]

在内陆干旱区,水资源是制约经济社会发展和生态安全的关键因素,而气候变化和人类活动加剧了水资源的不确定性.因此,发展和构建适合于内陆干旱区的水资源管理制度和模式是一个亟待解决的问题.在分析影响内陆干旱区水资源管理基础上,提出了实施最严格水资源管理的关键技术框架,包括“自然—社会”水循环模式和水资源综合模拟技术、水资源优化配置方法和分配体系、地面监测优化结合3S的“天地一体化”监测技术及水资源综合评价模型和利用考核评价体系等关键技术