The High Speed PCB Design of Console Core Board for Miniature NMR Spectrometer

核磁共振波谱仪是利用不同元素原子核性质的差异分析物质的磁学式分析仪器，广泛用于化合物的结构测定、定量分析和医学研究等方面。商用的核磁共振波谱仪虽然功能强大，但体积过大，价格过高限制了其在要求轻便灵活的场合中的应用。随着芯片的高度集成化和功能多样化，仪器设备也朝着小型化发展，无论是商用还是教学科研等场合对小型化核磁共振谱仪的需求也将越来越大。 本文以国家自然科学基金“基于电化学——核磁共振联用系统的研制及其应用”为课题背景，以核磁共振波谱仪的小型化为目标，提出一种小型化NMR波谱仪的方案，并着重对控制台核心板FPGA系统的硬件电路进行设计。 核心板FPGA系统以FPGA芯片为核心，包含了时钟...The NMR spectrometer is a kind of Magnetic analytical instrument which can analyse substance by the analysis of the difference between the nature of the different elements of the nucleus, widely used in the determination of structure of the compound, quantitative analysis and medical research. Although the commercial NMR spectrometer is powerful, its volume and price are too large and too high res...学位：工学硕士院系专业：物理与机电工程学院_电磁场与微波技术学号：1982010115279

Simultaneous determination of 7 nucleosides in Asterias rollestoni using reversed-phase high performance liquid chromatography

建立了罗氏海盘车中7种核苷化合物的反相高效液相色谱分析测定方法。采用超声波辅助提取,选用两根不同的C18色谱柱串联,以甲醇和0.2%(体积分数)乙酸/水溶液为流动相梯度洗脱分离。优化的色谱条件为:柱温为室温,检测波长为260nM,流速为0.8Ml/MIn,进样量为20μl。结果表明,7种核苷化合物在一定的浓度范围内线性关系良好,次黄嘌呤和胸苷的线性范围为0.65~40Mg/l,尿苷、黄嘌呤和肌苷的线性范围为0.80~40Mg/l,胸腺嘧啶的线性范围为1.15~40Mg/l,鸟苷的线性范围为0.50~40Mg/l。样品中7种核苷化合物的加标回收率为90.00%~105.00%,相对标准偏差为0.72%~3.23%。该方法操作简便、灵敏度高、重复性好,回收率高,适用于罗氏海盘车中7种核苷类成分的同时分析,也可用于罗氏海盘车的质量控制和综合评价。A method for the simultaneous determination of 7 nucleosides in Asterias rollestoni was devel-oped using reversed-phase high performance liquid chromatography ( RP-HPLC) .Analytes were extracted by ultrasonic-assisted extraction and separated on two different C18 columns,which were connected in se-ries,under the gradient elution with the mobile phases of methanol and 0.2% ( v/v) acetic acid/water at room temperature.The chromatographic conditions were as follows: flow rate,0.8 mL/min; detection wavelength,260 nm; injection volume,20 μL.Under the optimized conditions,good linear relationships between the values of mass concentrations and the peak areas of hypoxanthine,uridine,xanthine,thy-mine,inosine,guanosine and thymidine were observed in the ranges of 0.65-40,0.80-40,0.80-40,1.15-40,0.80-40,0.50-40,and 0.65-40 mg/L,respectively.The relative standard devia-tions were around 0.72%-3.23% and the recoveries were around 90.00%-105.00%.The results showed that the developed method is sensitive,accurate and reproducible.It is suitable for the analysis of nucleosides in Asterias rollestoni with high recoveries and it is expected to be used for the quality control and evaluation of Asterias rollestoni.国家自然科学基金项目(20905017);海洋公益性行业科研专项(200705011;200805039);海洋局青年基金项目(2010140);海洋一所基本科研业务专项(GY-022008T32;2010G25);中国科学院实验海洋生物学重点实验室开放基金课

Primary Exploration on Quality Evaluation of Holothurian Sold on the Market by HPLC Fingerprint

以10批不同产地刺参HPlC指纹图谱中的6个共有峰为评价指标,结合相似度分析,对不同刺参质量进行评价和检验。结果表明:不同批次刺参样品的6个色谱峰在指纹图谱分析过程中色谱行为相同,峰面积大,特征性强,能反映刺参的固有化学特征。结合相似度分析,可用于刺参质量评价。An optimized high performance liquid chromatography (HPLC) method was developed for the analysis of holothurian and the HPLC fingerprint was established from 10 batches of the holothurians from different habitats.The HPLC fingerprint showing 6 common characteristic peaks was used to explore the quality evaluation of holothurian and distinguish from the fakes with the similarity analysis.This method is accurate and reliable,providing a scientific basis for the quality control of holothurian and can be used to evaluate the quality of holothurian sold on the market.我国近海海洋综合调查与评价908专项(908-02-05-04):海洋药用生物资源评价和《中华海洋本草》编纂;海洋一所基本科研业务专项(GY-022008T32):海洋绿藻活性成分分析、鉴

Determination of five poisonous elements in holothurian sold on the market by Microwave Digestion with ICP—MS

建立了微波消解—电感耦合等离子体质谱法测定海参中Cu,AS,Cd,Hg,Pb 5种有毒元素含量的方法,采用In元素为内标,利用其回收率对以上5种元素的测定结果进行校正,该方法变异系数(CV%)为0.2%--4.0%,In元素加标回收率为96.7%--104.5%,说明该方法灵敏度高,结果准确可信。利用该方法对10批不同市售海参中5种有毒元素含量进行了测定,结果表明10批市售海参中的AS含量均超标,部分批次海参中的Cd、Pb超标,说明市售海参确实存在有毒元素超标的问题。A method based on microwave sample digestion and inductively coupled plasma-mass spectrometry(ICP—MS) detection was established for determination of Cu,As,Cd,Hg and Pb in holothurian sold on the market.Indium was used as internal standard element to calibrate the results of determination of these five elements.Determination limits of these five elements were in the range of 0.006～0.063 μg/g,coefficient of variation(CV%)were in the range of 0.2%～4.0%,and the recovery of In was in the range of 96.7%～104.5%.The method has been applied to determine the five poisonous elements in ten holothurian samples sold on the market.The results indicated that the over-standard problem of poisonous elements in holothurian sold on the market existed.The content of total arsenic was over standard in all the ten samples,but the over standard problem of lead and cadmium was only found in part of the samples.国家自然科学基金(20675021);中国近海海洋综合调查与评价908专项(908-02-05-04)资

通过双水相的构建解决超大孔聚(苯乙烯-二乙烯基苯)微球的弱聚集

利用反胶团溶胀法能够制备出孔径在100-500 nm范围内可控的超大孔聚(苯乙烯-二乙烯基苯)微球,在生物大分子分离及固定化酶领域具有独特的优势。但在制备过程中,由于孔道形成处油水界面的缺失,原本附着的稳定剂难以继续附着,此时微球之间因为反应过程的机械搅拌碰撞镶嵌会形成团聚堆积,即弱聚集。虽然弱聚集的微

Applications of HPLC-MS in the Rapid Identification of Components in Marine Medicinal Organisms

高效液相色谱-质谱联用(HPlC-MS)技术,将HPlC的高分离效能与MS的强大结构鉴定功能结合起来,已逐渐成为天然产物化学成分快速分离、鉴别的强有力手段。本研究采用高效液相色谱-电喷雾质谱联用(HPlC-ESI-MS)技术,成功建立了刺参皂苷快速鉴别方法,并对市售干刺参中的4种皂苷进行了鉴别。此外,应用该技术对罗氏海盘车中的13种化学成分进行了快速鉴别。为海洋药用生物化学成分快速鉴别提供了1种值得推广的新方法,为更好的开发利用海洋天然药物奠定了基础。In recent years,high performance liquid chromatography-mass spectrometry combined the high separation efficiency of HPLC with rapid identification function of MS had become a powerful tool for the rapid separation and identification of chemical components in natural products.In the present study,a high performance liquid chromatography-electrospray ionization mass spectrometry(HPLC-ESI-MS) method was developed for rapid identification of 4 saponin components in dry Holothurian sold on the market.On the other hand,13 compounds in Asterias rollestoni were also identified by HPLC-ESI-MS.The HPLC-MS method presented in this paper for the rapid identification of marine compounds was worth popularizing and to lay a foundation for the further exploration of marine natural products.国家自然科学基金项目(20675021;20905017);中国近海海洋综合调查与评价908专项项目(908-02-05-04);海洋局青年基金资

Design of NMR transmit pulse waveform based on FPGA

基于任意波形发生器的原理,采用ddS(dIrECT dIgITAl SynTHESIS)技术,以单片机控制芯片STC89lE51和fPgA芯片XC5VfX70T为基础进行硬件和逻辑设计,使用ISE10.1和MOdElSIM软件编写脉冲波形发生器的各个子模块和测试模块,利用上位机软件或者键盘模块进行设置,改变波形频率和相位,最终产生核磁共振谱仪所需的调频调相波形数据。仿真结果表明,该方案产生的信号波形可灵活实现频率和相位的更改,波形性能良好,对于核磁共振谱仪发射脉冲波形的设计提供了可靠的方法和思路。The hardware and logic design based on MCU control chip STC89LE51 and FPGA chip XC5VFX70T combines the principle of the arbitrary waveform generator with DDS(direct digital synthesis) technology.The sub-modules of pulse waveform generator and test modules are designed by ISE10.1 and Modelsim.Using the PC software or keyboard to set the frequency and phase of waveform,the ultimate frequency modulation and phase modulation waveforms data required by the NMR spectrometer can be generated.The simulation results show that the signal waveform designed by the program can have a good performance in the change of frequency and phase,providing reliable methods and ideas for the design of the transmit pulse waveform in the NMR spectrometer.国家自然科学基金(11175149)资助项

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel