Surface Activation of Amino Polystyrene Microbeads Using Bromoacetic Acid for Immobilization of Antibodies

E-mail:[email protected][中文文摘]分子固定化技术[1]是构造化学、生物敏感元件的重要内容。本文研究氨基聚苯乙烯微珠表面的溴乙酸活化及抗体固定化效率。在pH4.5 条件下，溴乙酸与氨基聚苯乙烯微珠混合反应，制备成表面溴乙酰化的微珠。然后活化的氨基微珠与抗体分子进行偶联。抗体微珠与荧光标记二抗作用后，在荧光显微镜下拍摄微珠的荧光图像，考察活化微珠连接抗体分子的效果。进一步工作将应用抗体微珠开展实际血清样品中的抗原检测。[英文文摘]In this work, surface activation of amino polystyrene microbeads using bromoacetic acid for immobilization of antibodies is presented. The activation efficiency was investigated via microscopic fluorescent imaging.论文工作得到了国家自然科学基金（No.20775065）、教育部高校博士点基金（No.20070384023）、 化学生物传感与计量学国家重点实验室（湖南大学）开放课题（No.2006021）、国家基础科学人才培 养基金（No.J0630429）项目资助

Advantages and Disadvantages of the University Entrance Exam Reform Integrating Arts and Science

不分文理高考不仅仅是高考文理科合并那么简单,它需要在科目改革的同时照顾到试题的改革,需要在不分文理的前提下照顾到不同学生的潜力倾向,需要以高考的改革来带动中小学教育的变革,因而是一项牵一发而动全身的社会系统工程。我们必须对改革中可能出现的诸多具体问题进行认真思考,并着力解决。Integrating arts and sciences in the university entrance exam is not just merging the arts and sciences.It needs to take the reform of the questions into count as well as the reform of the subjects. It also needs to consider the student's to different tendencies under the premise of integrating arts and sciences. It needs to drive changes in the primary and secondary education with the reform of university entrance examination. It is a social systems engineering. We need to carefully think about it and strive to resolve many of the specific problems in the reforming

中药沐足结合护理干预治疗护理人员下肢静脉曲张的效果观察

目的探讨中药沐足结合护理干预在下肢静脉曲张护士中的应用效果。方法选取某三甲综合性医院患有下肢静脉曲张的临床护士60例为研究对象,应用随机数字表法分为两组,各30例。两组研究对象均行基础护理,对照组采用医用的循序减压弹力袜进行治疗,观察组实施中药沐足结合护理干预措施。2个月后比较两组患者疗效。结果观察组研究对象静脉曲张恢复总有效率高于对照组,观察组研究对象经过3个疗程的治疗,痊愈12例,显效l3例,有效4例;对照组分别为痊愈5例,显效8例,有效9例;观察组、对照组临床治愈率和总有效率分别为40.00%、96.67%和16.67%、73.33%。差异有统计学意义(P<0.05)。结论中药沐足结合护理干预可以改善下肢静脉曲张的临床症状。福建中医药大学2016年临床专项校管课题(XB2016085

Mechanism of geniposide in improving free fatty acid metabolism in rats with non-alcoholic fatty liver disease

观察栀子苷改善非酒精性脂肪性肝病的效果,从游离脂肪酸探讨栀子苷调节非酒精性脂肪性肝病的作用机制。健康雄性Wistar大鼠40只,随机分为4组:正常组、模型组、栀子苷组和血脂康组,每组大鼠10只,正常组大鼠给予正常大鼠饲料喂养,其余3组大鼠均采用高脂饲料喂养以诱导非酒精性脂肪性肝病,造模时间为8周,从第5周起至第8周末,栀子苷组和血脂康组分别灌服相应的药物。记录大鼠体重、肝湿重、脂肪质量;用相应方法检测肝组织TG,FFA,FAS,AMPK,ACCase及Malonyl-Co A含量,血清CHO,LDL-C的含量,血清AST,ALT的活性;观察肝脏组织肉眼及病理变化(HE染色法)。结果显示,与正常组相比,模型组大鼠的体重;肝湿重;脂肪重;血清CHO,LDL-C,ALT,AST;肝组织TG,FFA,FAS,ACCase及Malonyl-Co A含量皆显著升高(P<0.01);肝组织AMPK活性显著降低(P<0.01),肝组织肉眼外观及病理切片脂肪变性明显,并出现炎症损伤;与模型组相比,栀子苷组大鼠体重、脂肪质量、肝组织FFA含量、血清ALT,AST活性均显著降低(P<0.01),肝湿重,肝组织TG,FAS,ACCase及Malonyl-Co A含量明显降低(P<0.05),肝组织AMPK活性明显增多(P<0.05),肝脏肉眼外观及病理学表现均有所改善;与模型组相比,血脂康组大鼠的肝湿重、脂肪质量、肝组织TG,FFA和血清LDL-C水平明显降低(P<0.05);与血脂康组相比,栀子苷组大鼠的体重、脂肪质量、肝组织FFA含量均显著降低(P<0.01),其他方面无明显差异。结果表明,栀子苷具有显著的改善高脂饮食诱导的大鼠非酒精性脂肪性肝病的药理效应;其改善大鼠非酒精性脂肪性肝病的游离脂肪酸代谢是通过调节"AMPK-ACCase-Malonyl-Co A-FFA"轴来实现的。To observe the effect of geniposide on non-alcoholic fatty liver disease( NAFLD),and discuss the mechanism of geniposide for NAFLD from the aspect of free fatty acid,forty healthy Wistar male rats were randomly divided into normal group,model group,geniposide and Xuezhikang group. The rats in normal group were fed with normal diets,and the rats in other 3 groups were given with high-fat diet for 8 weeks to induce the NAFLD models. From the week 5 to end of week 8,the rats in geniposide and Xuezhikang group were intervened with corresponding medicines. The body weight,liver wet weight,and fat weight of the rats were recorded.Visual and pathological changes in hepatic tissues were observed with HE staining. The contents of TG,FFA,FAS,AMPK,ACCase and Malonyl-Co A in hepatic tissue,contents of CHO and LDL-C in serum and activities of AST and ALT in serum were detected by using corresponding methods. The results showed that the body weight,liver wet weight,and fat weight of the rats,CHO,LDL-C,ALT and AST levels in serum,TG,FFA,FAS,ACCase and Malonyl-Co A levels in hepatic tissues of the rats in model group were significantly higher than those in normal group( P < 0. 01),while AMPK activity was significantly lower than that of the normal group( P < 0. 01),with obvious visual and pathological steatosis in hepatic tissues,and inflammatory injury occurred in model group. Compared with the model group,body weight of the rat,fat weight,levels of FFA in hepatic tissues,ALT and AST activities in serum,liver wet weight,TG,FAS,ACCase and Malonyl-Co A levels were significantly decreased in geniposide group( P < 0. 01),while the AMPK activity in hepatic tissues was significantly increased( P < 0. 05),with improvement in visual and pathological performance.Compared with the model group,liver wet weight,fat weight,TG and FFA levels in hepatic tissues,and LDL-C level in serum were significantly decreased in Xuezhikang group( P < 0. 05). Compared with Xuezhikang group,the body weight of rat,fat weight and FFA level in hepatic tissues were significantly lower in geniposide group( P < 0. 01),but with no significant difference in other aspects. These findings indicated that geniposide was highly effective in improving the pharmacological effect of NAFLD induced by highfat diet,and the mechanism was achieved through AMPK-ACCase-Malonyl-Co A-FFA axis.国家自然科学基金项目(81274155;81503529);; 福建省自然科学基金项目(2014J01374);; 福建省高等学校新世纪人才计划项目;; 厦门市科技计划项目(3502Z20134020);; 福建省中医药科研项目(WZPW201308

Do pheromones influence human behavior？

信息素是动物进行社会交流的重要媒介, 它是否同样影响着人类的行为尚无定论. 人类所具有的高度适应性的社会行为模式、驳杂多变的外分泌物、以及部分退化的嗅觉解剖结构, 使得对人类信息素的行为效应、物质组成以至神经机制的探索充满了希望与挑战. 现有的证据提示人体的气味在多个方面表现出类似信息素的效应, 包括触发固有行为、调节内分泌水平、传递社会信息、诱发情绪起伏甚至认知变化等等. 其中, 两种类固醇物质雄甾二烯酮和雌甾四烯被视为&ldquo;准人类性信息素&rdquo;, 二者依据接收者的性别和性取向而选择性地传递性信息、调节情绪与注意, 并对下丘脑产生特异性激活. 这些发现深化了对人类化学信号与人际交流的认识, 也为进一步的研究提供了铺垫.</p

The invention relates to a method for in-situ aseptic enrichment culture of polychlorella

本发明属于海洋微微型蓝藻的无菌培养技术领域，尤其涉及一种原位无菌富集培养聚球藻的方法。将聚球藻接种至SNAX培养基中在24‑26℃，1600‑2400 lx条件下培养至对数后期，再将培养的藻液避光培养24‑36 h使聚球藻始终处于分裂停滞期，而后向其中加入链霉素、硫酸庆大霉素、硫酸新霉素三种抗生素组成的混合抗生素，继续避光培养6‑12 h，使其完成生活史，而后将生长后聚球藻再按照上述方式进行培养直至培养液和聚球藻胞内细菌去除，即可实现对原位藻株无菌培养。本发明采用的混合抗生素处理方法成功从原位得到聚球藻无菌富集样品

信息素影响人类行为吗?

信息素是动物进行社会交流的重要媒介, 它是否同样影响着人类的行为尚无定论. 人类所具有的高度适应性的社会行为模式、驳杂多变的外分泌物、以及部分退化的嗅觉解剖结构, 使得对人类信息素的行为效应、物质组成以至神经机制的探索充满了希望与挑战. 现有的证据提示人体的气味在多个方面表现出类似信息素的效应, 包括触发固有行为、调节内分泌水平、传递社会信息、诱发情绪起伏甚至认知变化等等. 其中, 两种类固醇物质雄甾二烯酮和雌甾四烯被视为&ldquo;准人类性信息素&rdquo;, 二者依据接收者的性别和性取向而选择性地传递性信息、调节情绪与注意, 并对下丘脑产生特异性激活. 这些发现深化了对人类化学信号与人际交流的认识, 也为进一步的研究提供了铺垫

聚球藻对光合有效辐射适应性响应与调控机制研究进展

Synechococcus, the main group of marine picophytoplankton, would change their structure composition, physiological characteristics and carbon-fixation capacity responded to marine environmental variations, which is always an important scientific issue to assess the primary productivity of ecosystems and maintain the stability of ecosystems. Photosynthetically active radiation (PAR), as one of the most important environmental factors affecting plant life activities, not only restricts the growth of marine phytoplankton, but also determines the geographical distribution of marine Synechococcus. The existing results show that different phylogenetic clades of Synechococcus occupy different ecological niches, and pigment types and abundance exhibit a PAR-dependent geographical distribution. Moreover, the light-controlled culture experiment confirms that PAR could affect the growth rate, light-catching pigment contents and the expression of key gene of photosynthesis of Synechococcus. In this study, the adaptive response and regulatory mechanism of Synechococcus to PAR were summarized. Additionally, the concerned problems for future research were put forward for further understanding of theoretical study of phytoplankton adaptation to PAR

Advances in the interactions between marine picocyanobacteria and heterotrophic bacteria

Algae-bacteria interaction is among the key areas in marine ecology. Marine picocyanobacteria are ubiquitous primary producers in the aquatic habitat; they play vital roles in global carbon cycles and microbial food webs. Prochlorococcus and Synechococcus are the two most important genera of marine picocyanobacteria. Through field and culture research, complicated interactions between cyanobacteria and heterotrophic bacteria were verified. The material basis of their interactions includes organic carbon, vitamins, amino acids, and inorganic nutrients, which is mainly through reciprocal symbiosis. Marine picocyanobacteria provide great sources of organic carbon for heterotrophic bacteria through primary production. Consecutively, heterotrophic bacteria release inorganic nutrients through mineralization for absorption by picocyanobacteria. These crucial interactions guarantee the survival of picocyanobacteria. In this study, the main interactions between marine picocyanobacteria and heterotrophic bacteria were summarized. Additionally, the concerned problems for future research were put forward for further understanding of their ecological functions