Study on Controlled Release of Epirubicin in Drug Delivery System and Self-assembly of Polylactide in Emulsion

癌症是威胁人类健康的重大疾病之一,恶性肿瘤的治疗在很大程度上仍以化疗为主。表阿霉素是一种广谱的抗肿瘤抗生素类药物，对白血病和其他实体瘤的疗效与阿霉素相似，临床上主要剂型为注射用表阿霉素冻干粉。为了降低给药量，减小毒副作用，本研究开发了表阿霉素缓释制剂新剂型。聚乳酸（PLA）以其所特有的良好生物相容性和生物降解性而得到了广泛的研究，尤其是在生物医学领域。基于聚乳酸的物化性质以及缓释材料要求，本论文选择无定形态消旋聚乳酸（PDLLA）为囊材，采用复乳－溶剂挥发法进行抗肿瘤药物表阿霉素缓释制剂的研究。同时在缓释制剂研究过程中，偶然发现了在甘油存在时无定形PDLLA能自组装形成非球形微结构，并对其进行...Cancer is a main disease to threat the health of human being. Chemical therapy is an important method to treat it comparing with operation and radial therapy. Epirubicin is an anthracycline antibiotic and antitumor epimer of doxorubicin that has been used to treat a wide range of cancers. At equimolar doses epirubicin is less myelotoxic than doxorubicin and has a lower incidence of cardiotoxicity....学位：理学硕士院系专业：化学化工学院化学系_有机化学学号：20032505

老年人备灾现状及应对策略的研究进展

阐述国内外老年人的备灾现状,老年人应对灾害能力下降,备灾能力较低,应对灾害相关知识和教育缺乏,慢性疾病影响其备灾状态,其获取备灾信息途径和方式单一。提出需建立社区备灾组织、提供..

Establishment of MDCK cell lines which stably express visualable human neonatal Fc receptor

[目的]建立稳定表达融合EGFP的人新生儿Fc受体（h FcRn）的MDCK细胞株。[方法]构建重组慢病毒质粒p EGFP-h FcRn,采用四质粒包装系统共转染HEK 293T细胞生产重组慢病毒,感染MDCK细胞后对EGFP阳性细胞进行流式单细胞分选;通过Western Blot及EGFP-β2m荧光共定位验证h FcRn的完整性,并用流式细胞仪检测h FcRn与人Ig G的结合活性。[结果]测序结果表明成功构建p EGFP-FcRn慢病毒表达载体;感染后EGFP阳性MDCK细胞比例约为26.5%,流式单细胞分选后得到纯阳性细胞;荧光共定位及Western Blot均检测到h FcRn的完整表达;流式分析表明细胞株上的h FcRn与Ig G存在p H依赖性结合。[结论]成功获得稳定表达具有生物活性的可视化h FcRn的MDCK细胞株。[ Objective] To establish MDCK cell line stably expressing EGFP- human neonatal Fc receptor（hFcRn） fusion protein. [ Methods ] The lentiviral expression vector for EGFP - hFcRn fusion protein was constructed. Generating by co - transfection of four -plasmids into HEK 293T cells ,the lentivirus particles were used to infect MDCK cell line. EGFP positive single cell was obtained by FACS, and then FcRn expression was identified by fluorescence co -location with EGFP - β2m and confirmed by Western Blot. Flow cytometry was used to detect binding activity of hFcRn and human IgG. [ Results ] DNA se- quencing demonstrated that the lentivirus vector pEGFP - FcRn was constructed successfully. The percentage of EGFP - posi- tive ceils was about 26.5% after infection. Expression of the complete protein was detected through fluorescence co - location and Western Blot, respectively. Flow cytometry analysis showed that the cell lines could pH - dependently capture human IgG. [ Conclusion] MDCK cell line stably expressing functional visualable hFcRn was established.基金项目：国家自然科学基金项目（“结构生物学指导的HBV治疗性抗体人源化及其关键技术研究”,No.31600748;“抗呼吸道合胞病毒高中和活性抗体的保护机制研究”,No.81401668;“基于广谱中和单抗的通用型流感疫苗设计及其结构基础研究”,No.31670934

Identification of Irradiated Protein-rich Food by Dissociative Tyrosine

建立了高效液相色谱-游离酪氨酸法鉴定高蛋白含量辐照食品的方法。向经过辐照的高蛋白含量样品中加入0.1 MOl/l HCl,使细胞裂解并释放出游离酪氨酸,离心除去细胞碎片后,向上清液中加入80%丙酮并在-20℃下冰冻沉淀除去大部分蛋白,样品中的酪氨酸与三氟乙酸形成离子对后经C18色谱柱分离。在100~1 500μg/l质量浓度范围内,邻酪氨酸和间酪氨酸均具有良好线性,方法的回收率为61%~116%,定量下限(S/n≥10)为10μg/l。该法避免了蛋白质的水解,具有操作简便、测定准确等优点,能很好地区分未经辐照与经5 kgy以上剂量辐照的食品。A novel method was presented for identification of irradiated protein-rich food by HPLC with dissociative tyrosine.The procedure involved the homogenization of fresh sample with 0.1 mol/L HCl,followed by centrifugation to remove the cell debris and the solvent precipitation of protein with acetone at-20 ℃,then the tyrosine was separated by forming ion pair with trifluoroacetic acid on a C18 column.The average recoveries of the drugs ranged from 61% to 116% at the spiked levels of 100-800 μg/L.The method is simple and double-quick,and could be efficiently identified food sample irradiated at a dose of 5 kGy or above.国家质量监督检验检疫总局科研计划资助项目(2009IK183

Intratumoral Chemotherapy with an Implantable Collagen Film Impregnated with Epirubicin-loaded Polylactic Acid Microspheres Inhibits Tumor Growth in Hepatocellular Carcinoma Xenografts

[中文文摘]制备用于实体肿瘤局部治疗的植入型表阿霉素缓释药膜。采用复乳-溶剂挥发法制备聚乳酸载表阿霉素缓释微球,用交联复合法制备含载药微球的植入型胶原药膜;用扫描、透射电镜、共聚焦及粒度仪等考察微球和药膜的形貌、结构、粒径及体外释放;用H22肝癌荷瘤动物模型评价其体内抑瘤效果。结果:载药微球粒径分布均匀,外观圆整,平均粒径为5.81μm;微球的载药量4.39%,包裹率为37.2%;10h内载药微球在模拟体液中的累积释放率为35%;腹腔注射载药微球与瘤体局部植入胶原药膜对H22肝癌均有明显的抑瘤效果;微球注射与药膜植入两种不同给药方式对H22肝癌抑瘤效果也存在显著性差异(P<0.05)。植入型载表阿霉素缓释胶原膜具有良好的药物局部缓释特性,在肿瘤的术后局部治疗方面具有良好的临床应用前景。[英文文摘]Drug-releasing implants delivering chemotherapeutic agents can provide promising local therapy to patients with malignant disorders.The purpose of the present study was to develop implantable collagen film impregnated with epirubicin-loaded polylactic acid(PLA) microspheres(EPI-PM) for intratumoral administration in solid tumors.EPI-PM was prepared by double emulsion-solvent evaporation method,and the microspheres were further incorporated in biodegradable chemically cross-linked collagen to formulate implantable collagen film. The microspheres were characterized by scanning electron microscope , transmission electron microscope and laser confocus microscopy. In vitro release of epirubicin was examined by ultraviolet spectrophotometry. Transplantable murine hepatoma 22 (H22) model was used to evaluate the effects of EPI-PMor collagen film impregnated with EPI-PMon murine liver cancer.The results indicated that the epirubicin was well incorporated and dispersed homogeneously in PLA. The EPI-PM was uniformly spherical with average diameter of 5181μm, drug loading of 4139% , and embedding ratio of 3712%. The cumulative drug release rate of EPI2PM was 35 % in analog body fluid at the 10h. The tumor growth rate was significantly inhibited by intraperitonal injection of EPI2PMor intratumoral implantation of collagen film impregnated.国家重大科学研究计划项目(2006CB933300); 福建省自然科学基金(C0610045); 厦门市科技计划项目(3502Z20055007); 厦门市科技创新资金项目(3502Z20041029)

熔锥光纤倏逝场作用石墨烯双波长锁模掺镱光纤激光器

报道了利用熔锥光纤石墨烯饱和吸收体实现1μm波段的双波长锁模掺镱光纤激光器(YDFL)。利用光学诱导沉积法,将水溶液中的石墨烯纳米复合物在光倏逝场的作用下沉积至熔锥光纤的锥腰部位。该石墨烯熔锥光纤器件作为可饱和吸收锁模部件具有制作灵活、全光纤结构以及高损伤阈值等优点。基于该器件的可饱和吸收及腔内双折射滤波特性,通过调节偏振控制器优化激光腔内偏振特性,实现了稳定的YDFL双波长锁模。该激光器的两个锁模波长分别为1034.77nm和1038.85nm,二次谐波锁模重复频率为1.09MHz,腔内单脉冲能量最大可达35nJ

普鲁兰基肿瘤靶向性纳米粒子的制备、稳定性和体外释放

背景:普鲁兰多糖以其独特的优点在纳米递药系统领域受到越来越多的关注,但是,以普鲁兰多糖为材料进行改性制备的肿瘤靶向的纳米药物载体仍有待进一步研究与开发。目的:观察纳米粒子和载药纳米粒子的体外稳定性及所包载药物的释放特征,初步评价其作为纳米药物载体的潜力。方法:应用透析法制备乙酰普鲁兰叶酸偶合体纳米粒子,以表阿霉素为模型药物,制备乙酰普鲁兰叶酸偶合体/表阿霉素载药纳米粒子(FPA/EPI),应用储存法考察其稳定性,应用透析袋法观测体外释放特征。结果与结论:乙酰普鲁兰叶酸偶合体纳米粒子和FPA/EPI的粒径分别为(204.2±10.9)nm和(273.4±11.0)nm,在蒸馏水和体积分数10%胎牛血清中表面电位均较低,乙酰普鲁兰叶酸偶合体纳米粒子在水溶液中粒径1年内未见显著改变。载药纳米粒子对所包载的药物表阿霉素进行很好地释放,pH5.0磷酸盐缓冲液中释放速度明显高于pH7.4;乙酰普鲁兰叶酸偶合体纳米粒子和FPA/EPI制备容易,稳定性好,初步说明了两种粒子可望成为新型肿瘤靶向药物递药系统

基于差分统计方法的舌象纹理特征的分析与识别

为探讨舌诊客观化的方法,依据中医纹理判断的特点,针对舌象纹理特征分析,应用灰度差分统计方法,从对比度(CON) 、角度方向二阶矩(ASM) 、熵(ENT) 、平均值(MEAN) 四个参数,进行舌象纹理的量化和定义分类,并尝试将舌质老嫩的判别方法应用与临床舌质的判别,总体识别率为74 %。上海市教育青年基金资助项目(02CK22

Precise Measurement of Cosmic-Ray Proton and Helium Spectra with the BESS Spectrometer

We report cosmic-ray proton and helium spectra in energy ranges of 1 to 120 GeV and 1 to 54 GeV/nucleon, respectively, measured by a balloon flight of the BESS spectrometer in 1998. The magnetic-rigidity of the cosmic-rays was reliably determined by highly precise measurement of the circular track in a uniform solenoidal magnetic field of 1 Tesla. Those spectra were determined within overall uncertainties of +-5 % for protons and +- 10 % for helium nuclei including statistical and systematic errors.Comment: 12 pages, 4 figure