Preliminary Study on Introducing FMDV Epitopes Fused Gene into Alfalfa Mediated by Agrobacterium

口蹄疫是由口蹄疫病毒（Foot-and-mouthdiseasevirus，FMDV）引起的一种世界性的急性家畜传染病。其特点是传播途径广、传染性强、死亡率高等。 利用转基因植物生产疫苗是目前植物基因工程领域的一个研究热点，具有生产简单、成本较低、使用安全方便、易于贮存等优点。苜蓿以"牧草之王"著称，适应性广，可以在各种地形、土壤中生长，我国目前苜蓿的种植面积约133万hm2。苜蓿不仅产量高，而且草质优良，各种畜禽均喜食，是理想的口蹄疫转基因植物疫苗的载体植物。 本实验通过农杆菌的介导，以携带O型和A型口蹄疫抗原决定簇融合基因O21-O14-A21-HBcAg的植物表达载体转化苜蓿愈伤组织...Foot-and-mouth disease (FMD) is a world-wide infectious disease induced by foot-and-mouth disease virus (FMDV). It mainly infects the even-feet animals, and has the characteristics such as broad transmission, easy infection and high mortality. Vaccine derived by transgenic plants is becoming one focus of plant genetic engineering. There are such advantages as easy production, low cost, convenient...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：2012005130206

石墨烯/氧化石墨烯及其复合材料在污水处理中的应用研究进展

石墨烯(GN)材料作为一种新型的碳质吸附材料,具有耐高温、比表面积高以及良好的化学稳定性等特点,在污水处理方面具有很好的应用前景。综述了GN、形成GN的中间材料氧化石墨烯(GO)以及它们的复合材料在污水处理方面的研究进展。着重介绍了GN和GO的制备方法,分析讨论了GN和GO表面官能团与污水中重金属离子、染料和有机分子等污染物的相互作用机理及影响因素,并指出了GN和GO在环境修复方面的研究现状、存在的问题及发展方向,为未来GN和GO在污染物处理方面的应用提供了一些思路

绣球花状掺锶碳羟基磷灰石对Pb~(2+)的吸附

以NH_4H_2PO_4、Ca(NO_3)_2·4H_2O、Sr(NO_3)_2和尿素[CO(NH_2)_2]为原材料,通过水热法合成了一种单分散、由纳米片自组装而成的绣球花状三维(3-D)掺锶碳羟基磷灰石(Sr-CHAp),并用于吸附酸性水溶液中的铅离子(Pb~(2+)).研究了溶液p H、吸附时间、初始浓度对Sr-CHAp材料吸附Pb~(2+)的影响及在其在不同p H条件下的吸附机理.采用XRD、SEM、FI-IR和BET等技术对材料及吸附产物进行表征.结果表明,该吸附剂材料表面具有介孔结构,平均孔隙宽度是11.10 nm,具有较大的比表面积(43.54 m~2·g~(-1)),在pH3时对Pb~(2+)具有较高的饱和吸附量(985.1 mg·g~(-1));其等温线吸附数据符合Langmuir模型,动力学吸附数据符合伪二级动力学模型;在酸性溶液中其对Pb~(2+)的吸附主要表现为溶解/沉淀机理,溶液p H值的大小影响吸附后的产物,强酸性条件下有利于PbHPO_4的生成,而p H值为3—6时主要生成Pb_(10)(PO_4)_6(OH)_2

A study on transformation of fused gene O_(21)-O_(14)-A_(21)-HBcAg of FMDV epitopes into Medicago sativa

通过农杆菌的介导,以携带O型和A型口蹄疫抗原决定簇融合基因O21-O14-A21-HbCAg的植物表达载体转化苜蓿(MEdICAgO SATIVA)愈伤组织,并再生得到抗性植株。试验建立了苜蓿甘农1号愈伤组织再生系统;优化了农杆菌介导的苜蓿遗传转化系统;获得抗性再生植株4棵;guS报告基因在转化的愈伤组织中得到瞬时表达;抗性植株PCr检测结果证实目的基因已经成功整合到苜蓿基因组中。The fused gene O21-O14-A21-HBcAg that contained both type O and A of FMDV epitopes were introduced into alfalfa(Medicago sativa cv.Gannong No.1)leaf explants via Agrobacterium tumefaciens-mediated transformation.After infection and co-culture resistant plants were obtained from the resistant callus and the genetic transformation and regeneration system of alfalfa were established.The GUS reporter gene was transitorily expressed in transformed callus.52 regenerated individual plants were acquired through Hygromycin screening test.After PCR tests for transgenic plants,four out of seven randomly selected plants were positive.The results of PCR indicated that the O21-O14-A21-HBcAg gene was integrated into the genomes of transformed alfalfa plants.甘肃省教育厅研究生导师基金(0703-11);兰州理工大学博士基金(SB08200602

Extraction of Plasmid DNA by Reversed Micelles

采用新的TOMAC(甲基三辛基氯化铵)/2-乙基己醇/异辛烷反胶团体系,对该反胶团萃取质粒pUT649进行了研究.考察了表面活性剂浓度、离子浓度等对质粒DNA萃取的影响.当采用1.0%(volume)2-乙基己醇/异辛烷为有机相,TOMAC浓度为40 mmol.L-1,水相初始DNA浓度为25μg.mL-1时,质粒pUT649的萃取率可达90%以上.离子浓度对萃取过程有重要影响,反萃取时通过调节水相中的离子浓度,可以实现DNA和RNA的分离.研究结果表明,TOMAC/2-乙基己醇/异辛烷反胶团体系适合于核酸的萃取和纯化.Using plasmid pUT 649 as a model plasmid,the extraction of DNA by reversed micelles system of methyltrioctylammoniumchloride(TOMAC)/2-ethylhexanol/isooctane was studied.The effect of the main factors such as the concentration of TOMAC surfactant and ions in the aqueous phase was investigated.The degree of extraction of DNA was strongly influenced by the concentration of surfactant in the organic solvent and ionic strength i4n the aqueous phase.When the surfactant concentration reached 40 mmol/L,the extraction ratio of plasmid pUT 649 exceeded 90%,with an initial DNA concentration of 25 μg·mL~(-1) in aqueous phase.Electrostatic interaction between cationic surfactant and negatively charged DNA is the major driving force in the nucleic acid extraction,and the extraction ratio of nucleic acid decreased with the increased salt concentration,such a decrease is due to electrostatic hindrance by the salt.RNA could be simply separated from DNA with reverse micelles by adjustment of salt concentration.The results indicated that(TOMAC)/2-ethylhexanol/isooctane reversed micelles system was suitable for DNA separation and purification.留学回国人员基金;; 厦门大学新世纪优秀人才基金资

铅胁迫对不同湿地植物耐性和吸收的影响

人工湿地系统中湿地植物种类的选取是影响其去除重金属效率的关键因素。通过水培试验研究了Pb胁迫(CK,10 mg·L~(-1),20 mg·L~(-1))对18种挺水湿地植物的耐性及吸收的影响。结果表明:Pb胁迫条件下,供试湿地植物根部对Pb的积累能力较强,Pb被根系吸收后大部分被固定于根部,只有少量被转运至地上部分;不同湿地植物对Pb的吸收差异显著(P<0.01),Pb耐性..

Effects of lead stress on tolerance and uptake of different wetland plant species．

人工湿地系统中湿地植物种类的选取是影响其去除重金属效率的关键因素。通过水培试验研究了Pb胁迫(CK,10 mg·L~(-1),20 mg·L~(-1))对18种挺水湿地植物的耐性及吸收的影响。结果表明:Pb胁迫条件下,供试湿地植物根部对Pb的积累能力较强,Pb被根系吸收后大部分被固定于根部,只有少量被转运至地上部分;不同湿地植物对Pb的吸收差异显著(P<0.01),Pb耐性..