Design and Implementation of Forest Fire Prevention Decision-assisting System in a City

森林生态系统作为陆地生态系统中面积最大最多、最重要的自然生态系统，也是可持续发展的基础，森林生态系统对于经济发展是不可或缺的一部分，越来越多的人意识到森林生态系统的不可替代性。森林保护需要预防和消除对森林的各种破坏和灾害，最大限度避免和减少森林资源的损失，在这其中，森林火灾是森林保护工作者所要面临的重要难题。 论文设计与实现了一款森林防火指挥决策辅助系统，森林防火信息系统是为了达到森林防火的目的，借助譬如森林资源数据、防火信息以及卫星遥感等数据资源，以及成熟的3S技术、计算机技术，汇集防火信息管理、实时监控、指导扑救、提前测报、灾后补救处理为一体的综合应用体系。 论文首先概括的介绍了森林防...As the largest and the most important ecological system in the natural ecological system, forest ecological system is also the foundation for sustainable development. Forest ecological system is an indispensable for economic development, and more and more people realize the irreplaceable role forest ecosystem. Forest protection is to prevent the forest from destruction, and to reduce the loss of f...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201323160

Measurement of Sudan Dyes and Vitamins with HPLC and Online Photochemical Derivatization - Fluorescence Detection

光化学衍生是基于物质吸收一定波长的紫外或可见光辐射而引起的光化学反应而建立的一种分析技术。在线光化学衍生法相比传统的化学衍生有其独特的优势与特点，如对环境友好、操作简便、易实现自动化等，其与荧光分析法的结合大大拓展了荧光分析的应用范围，同时也为许多物质的低含量测定提供了新的技术，在药物分析、环境样品分析及复杂生物样品分析方面显示出广阔的应用前景。因此积极开拓适合该方法的目标物，或增强方法的实用性是具有重要的意义。本研究针对苏丹红和维生素的高效液相色谱（HPLC）分离–在线光化学衍生–荧光光谱测定方法进行了研究，并应用于实际样品的测定。研究论文分为四章： 第一章介绍了光化学衍生分析法的原理...Photochemical derivatization (PCD) is a technique for analyte transferring or chemical species reforming based on the light irradiation induced chemical reaction. Online photochemical derivatization has many advantages, such as environment-friendly, easily control and automation, compared with ordinary chemical derivatization. This technique has greatly expanded the application of traditional mole...学位：理学硕士院系专业：海洋与环境学院海洋学系_海洋化学学号：2242008115147

Determination of four Sudan dyes in chili oil by high performance liquid chromatography with on-line photochemical derivatization and fluorescence detection

建立了在线光化学衍生、荧光检测、高效液相色谱(HPlC)测定辣椒油中苏丹红Ⅰ、Ⅱ、Ⅲ和b的方法。以乙腈-水为流动相,采用梯度洗脱方式在Sb-C18色谱柱上分离。用实验室自制的程序控制时间/光强光化学反应器作为在线衍生装置,优化了光衍生反应的条件和荧光检测条件。3种不同加标浓度下,辣椒油样品中4种苏丹红染料的加标回收率为81.3%~100.4%。加标水平为0.8 Mg/kg下荧光信号强度的相对标准偏差(rSd,n=6)为2.6%~3.8%。苏丹红Ⅰ、Ⅱ、Ⅲ和b的检出限(lOd)和定量限(lOQ)范围分别为0.009~0.054 Mg/kg和0.030~0.181 Mg/kg,优于传统的HPlC分离、二极管阵列检测器检测方法。该方法具有简单、灵敏、选择性好的特点,适用于食品样品中苏丹红的常规分析。A method for the measurement of Sudan Ⅰ,Sudan Ⅱ,Sudan Ⅲ and Sudan B in chili oil using high performance liquid chromatography(HPLC) with on-line photochemical derivatization and fluorescence detection has been developed.The Sudan dyes were separated on an SB-C18 column in a single run by the mixed mobile phase of acetonitrile-water with a gradient program.A laboratory-built time/energy programmed photochemical reactor(PCR) with an ultraviolet mercury lamp was installed between a photodiode array detector(PDA) and a fluorescence detector(FLD),and it was applied to convert the non-or weakly fluorescent Sudan dyes into fluorescence emission components.The photochemical derivatization conditions and fluorescence detection parameters have been investigated and optimized.The recoveries of the standards spiked in real chili oil samples for all the dyes were 81.3%-100.4%.The relative standard deviations(RSDs,n=6) of the fluorescence signal intensity at the spiked level of 0.8 mg/kg were 2.6%-3.8%.The limits of detection(LODs) were in the range of 0.009-0.054 mg/kg and the limits of quantification(LOQs) were 0.030-0.181 mg/kg,which were better than those of the commonly used HPLC coupled with PDA.The developed method which is simple,sensitive,and selective can be applied to the routine analysis of Sudan dyes.美国Agilent公司高等学校研究基金项

The calculation results of the cyclic subgroup of the symmetry group

计算出S6,S7,S8,S9,S10,S11的循环子群,并给出其共轭分类.Calculated the cyclic subgroup of 6 7 8 9 10 11 S,S,S,S,S,S,and gave the conjugate classification

人嗅黏膜来源间充质干细胞对实验性自身免疫性脑脊髓炎小鼠的治疗作用与机制

【目的】研究人嗅黏膜来源的间充质干细胞（OMSC）对实验性自身免疫性脑脊髓炎（EAE）小鼠的治疗作用及机制。【方法】经鼻内镜局麻下取健康捐献者嗅区黏膜上皮，消化、传代后获取嗅黏膜间充质干细胞，培养至第5代，进行干细胞表面分子鉴定、多向诱导分化及染色。以髓鞘少突胶质细胞糖蛋白（MOG35-55）和百日咳毒素（PT）诱发C57小鼠EAE模型，记录小鼠神经功能评分（10分法）。造模后第16天（发病高峰期）将已发病小鼠随机分为治疗组和对照组，分别进行经尾静脉OMSC和等体积PBS治疗。造模后第24天经内眦静脉取血，CBA法检测小鼠血浆IL-10、IL-17、IFN-γ、IL-6水平。取脊髓，HE和LFB染色观察损伤灶大小。取健康捐献者外周血淋巴细胞与OMSC共培养，2d后比较单独培养组和共培养组分泌IFN-γ的CD4+T淋巴细胞（Th1细胞）比例；加入IDO、COX抑制剂干预，观察2d后各组Th1细胞比例。【结果】OMSC表面分子表达符合间充质干细胞特性并具有多向分化潜能。MOG35-55及PT成功诱导EAE模型，小鼠出现不同程度的神经功能损伤，OMSC治疗后，治疗组小鼠发病严重程度较对照组减轻（=0.002），且治疗组血清IFN-γ浓度低于对照组（=0.032），IL-10、IL-17、IL-6浓度差异无统计学差异。HE、LFB染色切片分别显示OMSC治疗组炎症因子浸润程度较对照组减轻，脱髓鞘区域范围也较对照组减小。人外周血淋巴细胞与OMSC共培养2d后，Th1细胞比例较淋巴细胞单独培养组降低（=0.001）。IDO抑制剂组Th1细胞比例高于共培养组（=0.01），与单独培养组的差异无统计学意义；COX抑制剂组Th1细胞比例与共培养组相比无统计学差异。【结论】OMSC可能通过IDO通路调控Th1淋巴细胞比例，从而抑制实验性自身免疫性脑脊髓炎小鼠的脱髓鞘损伤反应。本研究为临床治疗多发性硬化疾病提供了新的思路

间充质干细胞对B细胞增殖及免疫调节功能的影响

【目的】研究观察间充质干细胞（MSC）在体外培养体系对B细胞增殖和免疫调节作用的影响。【方法】采用密度梯度离心法分离健康捐献者来源的骨髓30mL。用含100mmol/L胎牛血清的L-DMEM培养基对分离出的MSC进行贴壁培养。培养至第6代时，进行多向分化和细胞表型鉴定。获取健康供者外周血单个核细胞，并通过流式细胞仪分选出CD19+B细胞，进行荧光染料CFSE标记，然后分成单独培养组（CD19+B细胞）和共培养组（按CD19+B∶MSC：=5∶1的比例共培养），两组均采用CpG+CD40L刺激，96h后检测B细胞的增殖情况，并通过流式细胞荧光分选技术（FACS）检测CD19+CD5+B细胞的百分比及其分泌的IL-10水平；进一步观察单独培养组和共培养组的CD19+B细胞对CD4+T细胞增殖以及分泌IFN-γ的影响，最后加入IL-10中和抗体，再次验证B细胞对CD4+T细胞增殖以及分泌IFN-γ的影响。【结果】健康捐献者来源的MSC具有多向分化能力，并表达CD29、CD44、CD73、CD90、CD105、CD166，不表达CD45和CD34。与单独培养组比较，共培养组中的CD19+B细胞与MSC在体外共培养3d后，CD19+B细胞增殖比例明显提高，并显著抑制CD4+T细胞分泌IFN-γ的功能。进一步分析CD19+B细胞的亚群，发现与单独培养组比较，共培养组中的CD5+B细胞数量的百分比由（21.31±1.22）%增加至（31.27±0.92）%（P=0.014）；而且其IL-10的分泌水平由（1.09±0.08）%增加至（2.44±0.06）%（P<0.001）。当在共培养组中加入IL-10中和抗体后，发现与MSC共培养的B细胞抑制CD4+T细胞增殖和分泌IFN-γ的功能的能力明显减弱。【结论】MSC能促进CD19+B细胞的增殖，同时具有增强CD19+B细胞抑制CD4+T细胞分泌IFN-γ，这可能与分泌IL-10的CD19+CD5+B细胞的表达增加相关

LARGE-SCALE EXPOSITION PLANNING AND MANAGEMENT OPTIMIZATION BASED ON VISITORS' BEHAVIOR SIMULATION:A CASE STUDY of QINGDAO INTERNATIONAL HORTICULTURAL EXPOSITION 2014

2014年青岛世园会参观人次众多,需要对参观者行为进行模拟分析,以保证活动安全,提升游客体验。研究提出了通过行为模拟支持规划评价、预警及对策的技术框架。利用路径调查与陈述偏好调查获得参观者行为数据并建立模型,进而在多个情景下对个体行游轨迹进行了模拟仿真,分析预测了参观者排队、密度等参数的时空分布情况。在针对不同类型的评价对象提出各自评价指标的基础上,从模拟结果中辨识可能存在的问题,并从规划与管理两方面提出预警及优化对策。研究证明了对大型展会活动开展参观者行为模拟的技术框架的可行性,其结果对本次世园会具有一定的实践指导意义。It is necessary to simulate visitors' behavior in Qingdao International Horticultural Exposition 2014 for security and high-quality visitor experience due to the huge estimated pedestrian volume.This paper proposes a technical framework in purpose of supporting the assessment, early warning as well as countermeasures based on behavior simulation.Visitors' activity data are collected through a route choice survey and the informants' reported preference, based on which the behavior models are estimated.Visitors' trajectories under several scenarios are simulated, together with the spatio-temporal distributions of visitors' density and queuing behaviors.Criteria are proposed to evaluate each simulated object, based on which indexes are set up, used to identify problems that are likely to happen in the real exposition.In the end, the paper proposes early warnings and countermeasures of both planning and administration.The simulation framework is demonstrated to be feasible and valuable for the exposition.国家自然科学基金项目“基于行为分析的商业综合体空间优化方法研究”的部分成

Figures' Cultural Implication Correspondence Between Chinese and English:A Key To Translating Figurative Expressions

同样的喻体在不同的文化中所传递的形象信息往往不同。实现原文喻体与译文喻体的内涵对接是翻译比喻性词语的有效方法。对接的原则是源语的喻体和目的语的喻体必须都传递一致的形象内涵。英汉(汉英 )翻译比喻内涵信息对接的前提在于 :译者能够找准与源语比喻中的喻体在目的语中相对应的文化负荷喻体 ,而不是表象的对应词The same figure may convey different image information in different culture. The implication correspondence of figures in source language with that in target language is an effective method for translating figurative expressions. The principle of this correspondence in C E translation is that figures in Chinese and figures in English should convey the same image information. Translators must learn to find correct culturally-loaded figures in English rather than literally-correspondent ones for the figures in Chinese if they want to translate figurative expressions well