The Design of Multi-functional Micro Electrical Machining Power Supply Based on CPLD

为了解决微能脉冲电源发展中遇到的功能单一、集成度不高等问题,设计了多功能微细电加工脉冲电源。以单片机和CPld作为微控制器,利用模块化的方法对关键模块进行设计,并运用QuArTuS软件对高频脉冲发生单元进行仿真,得到微细电火花和微细电化学的脉冲波形,通过对脉冲的切换实现了微细电火花和微细电化学的加工。In order to solve the problem such as function single,integration degree low,a multi-functional micro electrical machining pulse power supply was designed.The microcontroller based on MCU and CPLD is designed by the moduleir method,and by Quartus software,the high frequency pulse generating unit is simulated.And the pulse waveform of micro EDM and ECM is obtained.The processing of micro EDM and ECM is realized by switching the pulse

浅论我国大黄鱼产业转型升级之对策

经过30多年的研究与发展,我国大黄鱼产业已进入转型升级阶段。本文就加快大黄鱼原良种体系建设、规范养殖网箱布局和优化网箱结构、提高养殖经济效益与产品质量、加快全价人工配合饲料研发与推广应用、实施鱼病综合防控技术、提高产品加工率、实施品牌战略、进一步发挥行业协会作用、发掘大黄鱼文化等方面,论述了我国大黄鱼产业转型升级的对策。福建省科技重大专项专题(2016NZ0001-4);;中央引导地方科技发展专项(2017L3019);;现代农业产业技术体系专项资金(CARS-47);;福建省自然科学基金(2015J06019

The expression of TNFAIP8 in gastric cancer and its clinical significance

目的通过检测胃癌组织中肿瘤坏死因子α诱导蛋白8(TnfAIP8)的表达,探讨其与胃癌临床病理参数的关系及与胃癌发生发展的关系,揭示胃癌的发病机制。方法采用免疫组化SP法,检测50例胃癌以及50例正常胃粘膜中TnfAIP8的表达。结果 50例正常胃粘膜组织中TnfAIP8无表达,而在50例胃癌组织中TnfAIP8阳性表达13例,TnfAIP8阳性率为26%。低分化胃癌组TnfAIP8阳性表达率明显高于中分化腺癌组(P<0.05);Ⅲ期胃癌组TnfAIP8阳性表达率明显高于Ⅱ期胃癌组(P<0.05);有淋巴结转移组TnfAIP8阳性表达明显高于无淋巴结转移组;TnfAIP8在不同性别和年龄的胃癌组织中无差异。结论 TnfAIP8的表达与患者的性别和年龄无关,而与胃癌的组织学分级、胃癌的TnM分期和淋巴结转移有相关性。TnfAIP8在胃癌组织中表达率较正常胃粘膜升高,差异有统计学意义(P<0.05),提示TnfAIP8可能参与了胃癌的发生、发展。Tumor necrosis factor-α induced protein-8(TNFAIP8) is a recently discovered antiapoptotic molecule.Recent data have demonstrated that TNFAIP8 is involved in the regulation of apoptosis,cellular signaling cascade,tumor proliferation and invasion,as well as metastasis.In this study,we aimed to investigate the expression of TNFAIP8 in patients with gastric cancer and their relationships with clinicopathology.We detected the expression of TNFAIP8 in 50 cases of gastric cancer and 50 cases of normal gastric tissues with SP immunohistochemical.In gastric cancer,the expression of TNFAIP8 was located in cytoplasm and/or nucleolus of tumor cells;the expression of TNFAIP8 in patients with gastric cancer(26%) was higher than that in the normal gastric tissues(0%,P﹤0.05).And the expression of TNFAIP8 in poor differentiated adenocarcinoma of gastric was higher compared with that in well differentiated(P < 0.05),the same difference was fund between Ⅲ andⅡ stages of adenocarcinoma.However,the expression of TNFAIP8 was on significant difference among different person with different age and gender.The results suggest that the expression of TNFAIP8 is not related with patient gender or age,but related with the stage of disease.And the expression of TNFAIP8 may be involved in the generation and development of gastric cancer.福建省自然基金(2010D009

羟乙基壳聚糖抗人死亡受体5单链抗体纳米粒的制备、鉴定及体内抑瘤研究

目的为提高抗人死亡受体5单链抗体治疗肝癌的效果,探讨羟乙基壳聚糖抗人死亡受体5单链抗体纳米粒(GCS-aDR5ScFv)的制备、鉴定并研究其对小鼠肝癌H22模型的治疗效果及机制。方法用亲和镍柱层析法纯化抗人死亡受体5单链抗体(aDR5ScFv),采用离子凝胶法制备GCS-aDR5ScFv,应用扫描电镜来检测GCS-aDR5ScFv的外观形态,激光粒度分析仪分析纳米粒的粒径及粒径分布,并检测其表面Zeta电势。建立小鼠肝癌H22模型,用0.272 mg/ml GCS-aDR5ScFv隔天治疗并测量小鼠体质量及肿瘤大小,治疗2周。通过Western blot检测active-caspase8、active-caspase3及BAX表达。结果纯化的aDR5ScFv符合理论条带(Mr30 000),GCS-aDR5ScFv纳米粒形态均一,纳米粒径大小、Zeta电势和多分散指数表明GCS-aDR5ScFv稳定。与正常组相比,治疗组小鼠体质量显著差异,治疗组肿瘤体积和大小差异显著。Western blot检测active-caspase8、active-caspase3、BAX的表达水平上调。结论本研究制备的GCS-aDR5ScFv稳定,对小鼠肝癌H22模型具有抑瘤作用,其机制可能与active-caspase8、active-caspase3及BAX蛋白表达上调相关

LARGE-SCALE EXPOSITION PLANNING AND MANAGEMENT OPTIMIZATION BASED ON VISITORS' BEHAVIOR SIMULATION:A CASE STUDY of QINGDAO INTERNATIONAL HORTICULTURAL EXPOSITION 2014

2014年青岛世园会参观人次众多,需要对参观者行为进行模拟分析,以保证活动安全,提升游客体验。研究提出了通过行为模拟支持规划评价、预警及对策的技术框架。利用路径调查与陈述偏好调查获得参观者行为数据并建立模型,进而在多个情景下对个体行游轨迹进行了模拟仿真,分析预测了参观者排队、密度等参数的时空分布情况。在针对不同类型的评价对象提出各自评价指标的基础上,从模拟结果中辨识可能存在的问题,并从规划与管理两方面提出预警及优化对策。研究证明了对大型展会活动开展参观者行为模拟的技术框架的可行性,其结果对本次世园会具有一定的实践指导意义。It is necessary to simulate visitors' behavior in Qingdao International Horticultural Exposition 2014 for security and high-quality visitor experience due to the huge estimated pedestrian volume.This paper proposes a technical framework in purpose of supporting the assessment, early warning as well as countermeasures based on behavior simulation.Visitors' activity data are collected through a route choice survey and the informants' reported preference, based on which the behavior models are estimated.Visitors' trajectories under several scenarios are simulated, together with the spatio-temporal distributions of visitors' density and queuing behaviors.Criteria are proposed to evaluate each simulated object, based on which indexes are set up, used to identify problems that are likely to happen in the real exposition.In the end, the paper proposes early warnings and countermeasures of both planning and administration.The simulation framework is demonstrated to be feasible and valuable for the exposition.国家自然科学基金项目“基于行为分析的商业综合体空间优化方法研究”的部分成

Characterization and immunoprotective effect of SjIrV1,a 66 kDa calcium-binding protein from Schistosoma japonicum

钙结合蛋白是日本血吸虫生长发育不可缺少的蛋白,具有非常广泛而重要的功能。在课题组日本血吸虫体被表膜蛋白研究基础上,利用PCr技术克隆了中国大陆株日本血吸虫66 kdA钙结合蛋白(SJIrV1)编码基因的CdnA序列,blAST分析与菲律宾株日本血吸虫SJIrV1 CdnA编码序列一致,荧光定量PCr分析表明该基因在童虫和成虫期不同发育阶段均有表达,其中在35 d和42 d成虫中表达量较高,在42 d雌虫中该基因表达水平远高于42 d雄虫。构建重组表达质粒PET28A(+)-SJIrV1,在大肠杆菌中成功诱导表达,重组蛋白主要以可溶性形式存在,通过高效液相色谱法(rP-HPlC)以及串联质谱法(MS/MS)鉴定所获蛋白为目的蛋白SJIrV1。蛋白质印迹(WESTErn blOTTIng)分析结果显示重组蛋白能被感染日本血吸虫鼠血清和免疫鼠血清所识别,SJIrV1蛋白在虫体各发育阶段中均表达。免疫荧光染色实验观察表明SJIrV1主要分布在日本血吸虫成虫的表膜。应用重组蛋白免疫bAlb/C小鼠后,免疫鼠血清中检测到较高水平的特异性Igg、Igg1和Igg2A抗体。结果表明SJIrV1可能在日本血吸虫的生长发育过程中起着重要作用。Calcium-binding protein is an indispensable protein which performs extensive and important functions in the growth of Schistosoma japonicum.Based on our primary study on tegument surface proteins of S.japonicun,a cDNA encoding a 66 kDa calcium-binding protein of S.japonicum(Chinese strain) was cloned,sequence analysis revealed that it was identical with that of SjIrV1 of Philippines strains S.japonicum.The expression of SjIrV1 were detected by Real-time PCR,using cDNA templates isolated from 7,14,21,28,35 and 42 days worms and the results revealed that the gene was expressed in all investigated stages,and the mRNA level of SjIrV1 is much higher in 42 d female worms than that in 42 d male worms.The cDNA containing the open reading frame of IrV1 was subcloned into a pET28a(+) vector and transformed into competent Escherichia coli BL21 for expression.The recombinant protein was purified using a Ni-NTA purification system,and confirmed by high performance liquid chromatography(RP-HPLC) and tandem mass spectrometry(MS/MS).Western blotting analysis showed that recombinant SjIrV1(rSjIrV1) could be recognized by the S.japonicum infected mouse serum and the mouse serum specific to rSjIrV1,respectively.Immunofluorescence observation exhibited that SjIrV1 was mainly distributed on the tegument of the 35-day adult worms.ELISA test revealed that IgG,IgG1 and IgG2a antibodies are significantly increased in the serum of rSjIrV1 vaccinated mice.The study suggested that rSjIrV1 might play an important role in the development of S.japonicum.国家自然科学基金(No.31172315); 上海科技发展基金(No.12140902700); 中国博士后科学基金(No.2012M510630)资助~

Identification and preliminary analysis of a novel full-length cDNA encoding retinoid X receptor 2 from Schistosoma japonicum

通信作者 E-mail: [email protected][中文文摘]目的克隆编码日本血吸虫视黄酸X受体2(SjRXR2)蛋白的全长cDNA,并对其进行初步研究。方法利用cDNA末端快速扩增技术(RACE)获得SjRXR2蛋白全长编码cDNA。利用生物信息学技术,对基因结构进行初步分析。利用实时荧光定量(Real time)PCR技术对该基因在日本血吸虫不同时期虫体中的转录情况进行分析。应用在线抗体表位预测软件获得SjRXR2配体结合区抗原性较强的一个多肽序列,合成该多肽片段,并免疫小鼠制备抗血清。利用Western blot技术分析该蛋白在日本血吸虫中的表达。结果采用RACE技术成功获得了SjRXR2蛋白全长编码cDNA,总长度为5 960bp,其完整开放阅读框为4 308 bp,编码1 435个氨基酸,预测分子量为159 kDa。生物信息学分析表明该基因编码的蛋白质序列具有核受体家族2的典型结构域特征,且与曼氏血吸虫RXR2有较高的相似性。Real time PCR分析表明,该基因在21、42 d龄日本血吸虫虫体内有较高的转录水平。Western blot分析表明,小鼠SjRXR2多肽免疫血清可特异性识别日本血吸虫虫体150 kDa蛋白。结论成功获得了编码SjRXR2蛋白的全长 cDNA，并制备了针对该蛋白的特异性多克隆抗体，为进一步研究该蛋白的功能奠定了基础。 .国家自然科学基金（31172315）；公益性行业（农业）科研专项（20090303036

Deficiency and Construction of the “Procedural Justice” in the University Constitution

大学章程被称为"高校宪章",是大学的根本法。依据章程实施办学和管理,是现代法治在大学治理中的具体体现。大学章程不仅是一部"实体法",更应是一部"程序法",它通过明确、合理的文本规定,制定了大学各利益相关主体实施权力和履行职责的程序,规范着大学场域的秩序。然而,在目前我国颁布的大学章程文本中,在权力规范和权利保护方面存在着明显的程序缺失,导致了大学章程难以落实和地位虚化,阻碍了其大学治理之功能。要充分发挥大学章程在高校依法治校中的作用,就必须彰显现代法治的"程序正义"精神。The university statutes,also called"the university Charter",is the university's fundamental law.Running according to regulations is the concrete embodiment of the modern administrative law in university governance. The University constitution is not only a substantial law,but also a procedural law,which sets out the procedures of the rights as well as the duties of the related bodies,through the explicit and rational rules. However,there are some serious procedural flaws about the standard and protection in the current university constitution,which makes it hard to put into practice and hinder the function. Therefore,in order to bring into full play of the university statutes,we must practice the "procedural justice"of modern administrative law.教育部人文社会科学重点研究基地重大项目“大学创新教学理论与实践机制构建”(15JJD880014

国产钨基催化剂在烯烃歧化制丙烯工业侧线装置上的性能研究

针对丁烯和乙烯歧化增产丙烯技术(OCT技术)所用进口BASF催化剂价格昂贵、运输条件苛刻等情况,自主研发了钨基歧化催化剂,并在实验室小试装置和OCT工业侧线固定床反应器上,考察了该催化剂在OCT技术制备丙烯过程中的催化性能。结果表明,1-丁烯的异构化率和丙烯的选择性均随反应温度的升高和乙烯、丁烯摩尔比的增加而逐渐增加,自主研发催化剂的催化活性和进口催化剂相当,且催化剂稳定性良好,可连续运行400 h以上