Applying Two -peak Ratio Discriminant Analysis on Autofluorescence Spectra to Identify Adenocarcinoma Colonic Tissues

目的:分别测量了人体正常和腺癌结肠组织在300 nM、340 nM、400 nM和460 nM波长激发下的自体荧光光谱,通过对测量的自体荧光光谱分析比较,探究临床上可以用于诊断早期结肠腺癌的最佳激发波长。方法:1人体离体结肠组织样品由福建省立医院肿瘤外科提供,离体样品均在2小时内完成光谱测量。2实验采用flS920(EdInburgHInSTruMEnTS,uk)稳态荧光光谱系统对结肠组织进行自体荧光光谱测量。3实验数据利用OrIgIn 7.5系统软件进行平滑归一化,采用双峰比值法计算不同激发波长下自体荧光发射峰的荧光积分强度。结果:采用双峰比值法计算得到人体结肠组织在340 nM波长激发下(380±20)nM和(485±20)nM发射峰的荧光积分强度比值存在显著差异(P<0.001),识别的灵敏度和特异性分别为80%和73.3%。结论:结果表明340 nM可作为诊断结肠腺癌的最佳激发波长。文章的初步结论可为光诱导人体结肠组织自体荧光诊断早期结肠癌提供了一定的参考价值。Objective: To investigate the differences between normal and adenocarcinoma colonic tissues autofluorescence spectra excitated by 300 nm、340 nm、400 nm and 460 nm,respectively.The optimal diagnosis excitation wavelength used in early adenocarcinoma colonic tissues was obtained through the measurement of autofluorescence spectra.methods: ①All samples of human colonic tissues in vitro were measured in two hours,which provided by the department of surgical oncology of Fujian provincial hospital.②A FLS920 fluorescence detection system was used to collect the emission spectra excitated by 300nm 、340nm 、400 nmand 460 nm,respectively.③After smoothed and normalized by Origin 7.5 system software,the algorithms of "two-peak ratio" were employed to distinguish normal and adenocarcinoma colonic tissues.Results: The ratio of two peaks at(380±20) and(485±20) nm under the 340 nm excitation excited sufficient difference(P<0.001),which the sensitivity was 80% and specificity was 73.3%.Conclusions: The results showed that 340 nm excitation was the optimum diagnostic wavelength.In conclusion,our results can provide a potential value in early diagnosis of human colonic tissues.三明学院自然科学基金项目(编号B201009/Q

The Influence Mechanisms of Organizational Structure on Counterproductive Work Behavior——From the Perspective of Psychological Climate

在回顾前人研究的基础上,基于员工心理气氛的视角,分析了组织结构的四个重要维度对员工反生产行为的影响机理。初步提出了一个中介效应理论模型及其相应的研究命题,并对未来理论和实证研究的方向提供了相应的建议。Based on the review of previous studies,this paper analyzes the influence mechanisms of organizational structure on counterproductive work behavior from the perspective of employee psychological climate.It builds up a mediating effect theoretical model and its corresponding research proposition.Meanwhile,points out the direction for further theoretical and empirical research.国家自然科学基金项目(71272206); 教育部人文社会科学青年基金项目(13XJC630012); 教育部人文社会科学规划基金项目(11YJA630057

组织内社会网络影响个体工作绩效的机理——基于角色外行为视角的理论研究

大多数关于个体工作绩效影响因素的研究都聚焦于个体特征和态度层面的微观变量,却忽视了组织特征层面的宏观变量。从角色外行为的视角分析组织内社会网络对个体工作绩效的影响,并提出社会网络影响个体工作绩效的机理,为后续的实证研究提供必要的理论基础

The Organizational Control Strategy against Employee Counterproductive Work Behavior:An Empirical Research from Social Cognitive Perspective

本文基于社会认知的视角探讨中国企业员工反生产行为(CWb)的组织控制策略,采用跨层次分析的方法对27家企业的426名员工进行了实证研究。结果发现:虽然中国企业员工的尽责感和神经质人格特质对其CWb具有显著影响。但是,由于自利和规则导向的组织伦理气氛以及领导公正和信息公正作为重要的组织情境,不仅可以直接对CWb产生显著影响,而且还对人格特质与CWb之间的关系起到复杂的调节作用。因此,中国企业可以综合采用员工人格特质测试、组织伦理气氛培育以及组织公正环境建设等策略来进行员工CWb的组织控制。This article investigate the organizational control strategy of Chinese enterprises employee's counterproductive work behavior( CWB) from the perspective of social cognitive,using cross-level analysis method to do empirical research based on 426 employees from 26 enterprises.The results show that although Chinese employee's conscientiousness and neuroticism personality trait have the significant personality,but instrumentalism and rule oriented ethical climate,leader justice and information justice are very important organizational context,they not only have significant affect to CWB respectively,but also complex moderate the relationships between personality trait and CWB.So,Chinese enterprises can use a variety of strategies to control the employee's CWB such as employee personality test,organizational ethical climate cultivation,organizational justice environment construction,and so on.国家社会科学基金项目(14CGL018); 教育部人文社会科学规划基金项目(11YJA630057); 国家自然科学基金项目(71302175

珠穆朗玛峰地区东绒布冰川冰雪微生物群落及其季节变化

通过流式细胞计数和构建环境样品16S rRNA基因的克隆文库,分析了珠穆朗玛峰地区东绒布冰川冰雪微生物数量和菌群结构特征及其与季节变化的关系．珠峰地区冰雪微生物的16S rRNA基因序列分属于α,β,γ-变形菌纲,放线菌门,厚壁菌门,CFB,蓝细菌,真核质体,待定门TM7共9大类,以γ-变形菌纲为主要类群,其中Acinetobacter和Leclercia属是整个菌群中的优势属．微生物的数量和菌群结构具有明显的季节特征,夏季微生物的数量高于冬季;菌群结构具有一些共有属种的同时,在夏、冬季雪中具有各自特有的属种,共有属种推测是青藏高原局地源的微生物,序列同源性分析结果表明,夏季较多细菌属种与海洋环境相关,冬季细菌则具有更强的耐冷性．微生物明显的季节变化可能是受珠峰地区夏季和冬季不同水汽来源的影响

Anti-resonance features of destructive quantum interference in single-molecule thiophene junctions achieved by electrochemical gating

基于单个有机分子来构筑电子器件为电子器件微型化提供潜在技术方案。本研究发展了可集成电化学门控的单分子电子器件测试芯片技术和科学仪器方法，在实验和理论两个层面对具有相消量子干涉效应的噻吩衍生物分子器件的电输运过程进行了电化学调控研究，从而首次在室温下实现了对单分子电子器件中量子干涉效应的反共振现象的直接观测和调控，为制备基于量子干涉效应的新型分子材料和器件提供了全新的设计思路和策略。该研究充分展示了电化学调控技术在信息材料和器件领域的重要应用潜力，也体现了我校固体表面物理化学国家重点实验室在电化学研究和科学仪器研发领域的技术积累，以及面向科学前沿开展交叉学科探索的研究特色。 该研究工作是在洪文晶教授、上海电力大学陈文博教授、英国兰卡斯特大学Colin Lambert教授指导下完成的。化学化工学院博士生白杰和李晓慧为论文的共同第一作者，刘俊扬副研究员、师佳副教授、研究生唐永翔、刘帅、黄晓娟、谭志冰和萨本栋微纳研究院的杨杨副教授等也参与了研究工作。田中群教授和毛秉伟教授为该工作提供了重要指导。【Abstract】Controlling the electrical conductance and in particular the occurrence of quantum interference in single-molecule junctions through gating effects, has potential for the realization of high-performance functional molecular devices. In this work, we used an electrochemically-gated, mechanically-controllable break junction technique to tune the electronic behaviour of thiophene-based molecular junctions that show destructive quantum interference (DQI) features. By varying the voltage applied to the electrochemical gate at room temperature, we reached a conductance minimum that provides direct evidence of charge transport controlled by an anti-resonance arising from DQI. Our molecular system enables conductance tuning close to two orders of magnitude within the non-faradaic potential region, which is significantly higher than that achieved with molecules not showing DQI. Our experimental results, interpreted using quantum transport theory, demonstrate that electrochemical gating is a promising strategy for obtaining improved in-situ control over the electrical performance of interference-based molecular devices.This research was supported by the National Key R&D Program of China (2017YFA0204902), National Natural Science Foundation of China (21722305, 21673195, 21503179, 21703188), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, Natural Science Foundation of Shanghai (17ZR1447100), Science and Technology Commission of Shanghai Municipality (14DZ2261000), China Postdoctoral Science Foundation (2017M622060) for funding work in Xiamen. It was also supported by EU Horizon 2020 project QuIET under grant agreement no. 767187EC FP7 ITN ‘MOLESCO’ project no. 606728 and UK EPSRC grants EP/N017188/1 and EP/M014452/1 and Leverhulme Trust (Leverhulme Early Career Fellowships no. ECF-2017-186 and ECF-2018-375) for funding instrumentation used in Lancaster. It was also supported by Hungarian and Czech Academies of Sciences (P2015-107) and Hungarian Research Foundation (OTKA 112034) for funding instrumentation used in Hungary. The authors thank Z.-Q. Tian and B.-W. Mao, Xiamen University, for useful discussions. 该工作获得科技部国家重点研发计划课题（2017YFA0204902），国家自然科学基金委优秀青年科学基金等项目（21722305、21673195、21703188、21503179）以及中国博士后科学基金（2017M622060）等项目的资助，也得到了固体表面物理化学国家重点实验室、能源材料化学协同创新中心的支持

Identifying the Conformational Isomers of Single-Molecule Cyclohexane at Room Temperature

构象异构是化学中的基本问题。然而对于环己烷等柔性分子，由于其在室温下极快的互变异构过程，基于系综的表征方法（如核磁等）只能得到所有构象平均贡献的结果。为了应对这一挑战，化学化工学院洪文晶教授与夏海平教授课题组为在室温条件下对柔性分子构象的定量分析与表征这一挑战，课题组成功实现了在室温条件下对环己烷两种椅式构象的电学表征与比例识别。同时，通过纳米电极间隙对分子的限域作用，发现在宏观尺度下极不稳定的扭船式中间体得以在单分子尺度稳定存在，这为不稳定中间体的研究提供了重要表征方法。 这一研究工作是在化学化工学院洪文晶教授、夏海平教授共同指导下完成的，iChEM直博生唐淳与化工系研究生唐永翔为论文共同第一作者。师佳副教授与刘俊扬副研究员为该工作提供了指导，博士后陈志昕、博士研究生陈李珏以及研究生叶艺玲、严哲玮、张珑漪共同参与了该工作。【Abstract】Isomerism reflects the ubiquitous nature that molecules with the same molecular formula show different structures. The interconversion between conformational isomers of flexible molecules is quite fast owing to the low barriers of around 10 kcal mol−1, leading to average signal contributed by all the possible isomers characterized by ensemble methods. On this account, identifying the conformational isomers of flexible molecules at room temperature has a substantial challenge. Here, we develop a single-molecule approach to identify the conformational isomers of cyclohexane at room temperature through the single-molecule electrical characterization. By noise analysis and feature extraction of the conductance of single-molecule junctions, we quantificationally identified two chair isomers of cyclohexane at room temperature, while such identification is only feasible at low temperatures by ensemble characterization. The strategy to apply the single-molecule approach to identify conformational isomers paves the avenue to investigate the isomerization of flexible molecules beyond the ensemble methods.This work was supported by the National Natural Science Foundation of China (nos, 21722305, 21673195, 21703188, and U1705254), the National Key R&D Program of China (2017YFA0204902), China Postdoctoral Science Foundation (no. 2017M622060), and the Fundamental Research Funds for Xiamen University (20720190002).该工作获得了科技部国家重点研发计划、国家自然科学基金等项目的资助，也得到了固体表面物理化学国家重点实验室、能源材料化学协同创新中心的支持

Room-temperature quantum interference in single perovskite quantum dot junctions

钙钛矿材料由于其高量子产率、载流子迁移率和独特的光致发光特性而在光电材料领域存在诸多潜在的重要应用。研究钙钛矿材料在纳米尺度下电荷输运的独特尺寸效应对钙钛矿光电器件的设计和开发具有重要的指导意义。洪文晶教授课题组基于机械可控裂结技术自主研发了具有皮米级位移调控灵敏度和飞安级电学测量精度的精密科学仪器，对南开大学李跃龙副教授团队合成的钙钛矿量子点进行了深入表征，研究工作成功将量子干涉的研究体系拓展至在光电领域具有重要应用的钙钛矿材料领域，为未来制备基于量子干涉效应的新型钙钛矿器件提供了一种全新的思路。 这一跨学科国际合作研究工作是在化学化工学院洪文晶教授、英国Lancaster 大学物理系Colin J. Lambert教授以及南开大学电子信息与光电工程学院李跃龙副教授的共同指导下完成的。化工系硕士研究生郑海宁、Lancaster University大学Songjun Hou博士、南开大学硕士研究生辛晨光为论文第一作者。博士后林禄春，博士研究生谭志冰、郑珏婷，硕士研究生蒋枫、张珑漪，本科生何文翔、李庆民等参与了论文的研究工作。刘俊扬特任副研究员、师佳副教授和萨本栋微纳米研究院杨扬副教授也参与了部分指导工作。The studies of quantum interference effects through bulk perovskite materials at the Ångstrom scale still remain as a major challenge. Herein, we provide the observation of roomtemperature quantum interference effects in metal halide perovskite quantum dots (QDs) using the mechanically controllable break junction technique. Single-QD conductance measurements reveal that there are multiple conductance peaks for the CH3NH3PbBr3 and CH3NH3PbBr2.15Cl0.85 QDs, whose displacement distributions match the lattice constant of QDs, suggesting that the gold electrodes slide through different lattice sites of the QD via Auhalogen coupling. We also observe a distinct conductance ‘jump’ at the end of the sliding process, which is further evidence that quantum interference effects dominate charge transport in these single-QD junctions. This conductance ‘jump’ is also confirmed by our theoretical calculations utilizing density functional theory combined with quantum transport theory. Our measurements and theory create a pathway to exploit quantum interference effects in quantum-controlled perovskite materials.This work was supported by the National Key R&D Program of China (2017YFA0204902, 2014DFE60170, 2018YFB1500105), the National Natural Science Foundation of China (Nos. 21673195, 21503179, 21490573, 61674084, 61874167), the Open Fund of the Key Laboratory of Optical Information Science & Technology (Nankai University) of China, the Fundamental Research Funds for the Central Universities of China (63181321, 63191414, 96173224), and the 111 Project (B16027), the Tianjin Natural Science Foundation (17JCYBJC41400), FET Open project 767187—QuIET, the EU project BAC-TO-FUEL and the UK EPSRC projects EP/N017188/1, EP/M014452/1. 该工作得到国家重点研发计划课题（2017YFA0204902）、国家自然科学基金（21673195、21503179、21490573）、厦门大学“人工智能分析引擎”双一流重大专项等项目的资助，也得到了固体表面物理化学国家重点实验室、能源材料化学协同创新中心的支持

Empirical Study on Resource Consumption, City Size and Economic Growth in Guangxi Based on Demand Perspective

基于需求视角,利用广西2002—2017年14个地级市面板数据对广西资源消耗、城市规模与经济增长之间的关系进行实证,研究发现:广西资源消耗与经济增长呈正相关关系,即广西存在"资源福音",但城市规模的扩大抑制了资源消耗。进一步考察广西资源消耗与经济增长、资源消耗与城市规模之间的关系,发现均呈倒"U"型关系。为驱动广西经济新增长,需要适当控制城市规模扩张、合理勘探与开发新资源并开展国际合作。Based on demand perspective, by using Guangxi’s 14 cities panel data in 2002-2017, this research elaborates the relationship between resource consumption, city size and economic growth. The study found that: the relationship between resource consumption and economic growth in Guangxi is positive under the demand perspective, which is called “resource blessing” in Guangxi, but the expansion of city size would hinder the resource consumption. After further empirical study on resource consumption and economic growth, resource consumption and city size, all result showed that there is an inverted u-shaped relationship. In order to achieve the new economic growth in Guangxi, it put forward some measures such as appropriately control of the city size expansion, rational exploration and development of new resources, and international cooperation.2013年国家社会科学基金项目“新形势下我国能源安全保障、环境保护与经济稳定增长的协同与政策优化研究”(13CJY044

寻求内源发展：中国西部的民族与文化

本书通过对中国藏区的发展、新疆与甘肃的畜牧业综合发展和青海省人与自然和谐发展的三个项目评估报告，结合项目区各少数民族社会和文化现状，探索西部内源发展和文化资本彰显之路