Changes in expression and localization of heat shock protein 70 during curcumin-induced apoptisis of human esophageal cancer cell line EC9706

目的探讨姜黄素对人食管癌EC9706细胞凋亡的诱导作用,热休克蛋白70(HSP70)在肿瘤细胞凋亡过程中在核基质上的变化及其与凋亡调控相关蛋白的关系。方法用细胞计数和流式细胞仪检测姜黄素对人食管癌EC9706细胞的增殖抑制作用,以光学显微镜和透射电镜观察姜黄素诱导人食管癌EC9706细胞凋亡前后的细胞结构变化,琼脂糖凝胶电泳观察人食管癌EC9706细胞凋亡前后的dnA结构变化。双向凝胶电泳和质谱鉴定分析HSP70在核基质中的存在与变化;并以WESTErn blOTTIng进行确证;激光扫描共焦显微镜观察HSP70在EC9706细胞凋亡过程中的定位及其与bAX、bCl-2等基因产物的共定位关系。结果姜黄素能显著抑制人食管癌EC9706细胞增殖并诱导人食管癌EC9706细胞凋亡,双向凝胶电泳、质谱鉴定和结果发现并证实,HSP70在姜黄素处理前后的EC9706细胞核基质蛋白中的存在及其表达下调变化。激光扫描共焦显微镜观察结果显示,HSP70在EC9706细胞凋亡过程中与bAX、bCl-2等基因产物具有共定位关系,且其共定位区域发生了变化。结论姜黄素对人食管癌EC9706细胞具有显著的凋亡诱导作用;HSP70作为一种新发现的核基质蛋白,在姜黄素诱导人食管癌EC9706凋亡过程中的表达与分布发生了显著变化。HSP70与凋亡相关基因的关系对EC9706细胞凋亡具有重要影响。Objective To investigate the effect of curcumin on human esophageal cancer EC9706 cells and explore the role of heat shock protein(HSP70) in cell apoptosis by examining changes in the nuclear matrix and its relationship with apoptosis-related proteins.Methods Cell counting and flow cytometry were performed to probe the inhibitory effect of curcumin on cellular proliferation.Transmission electron microscopy and optical microscopy were used to observe the structural changes in EC9706 cells before and after apoptosis.Agarose gel electrophoresis was conducted to investigate the DNA structure of EC9706 cells before and after apoptosis.Two-dimensional polyacrylamide gel electrophoresis(2-D PAGE) and mass spectrometry(MS) analysis were performed to investigate the presence and changes of HSP70 in the nuclear matrix of EC9706 cells before and after curcumin treatment,which was further corroborated by Western blotting assay.Laser confocal scanning microscopy was used to observe the colocalization of HSP70 with Bax and Bcl-2 during apoptosis.Results The results indicated that curcumin could markedly inhibited EC9706 cell proliferation and finally induced apoptosis.Data from 2-D PAGE,MS,and Western blotting showed that HSP70 was involved in the nuclear matrix proteins and expression of HSP70 was downregulated after curcumin treatment.Laser confocal microscopy showed that HSP70 colocalized with Bax and Bcl-2,and the colocalized regions were altered by the curcumin treatment.Conclusion Our work proves that curcumin could definitely induce EC9706 cells into apoptosis.As a new found nuclear matrix protein,the expression and distribution of HSP70 are altered during the apoptosis of EC9706 cells.The colocalization of HSP70 with apoptosis-related genes evidently affects the apoptosis of EC9706 cells.福建省自然科学基金资助项目(2011J01256); 中央高校基本科研业务费专项资金(2011121061

激光等离子体反应产生的磷银络离子

络合物离子的生成与研究,传统上是溶液化学的内容。我们却在自制的仪器上,以激光等离子体反应产生了多种AgP_n~+络离子,记录了它们的飞行时间质谱。实验装置的原理与构造已有另文介绍。实验时,将纯化后的红磷研细,与银粉均匀混合后压片。作用于该样品的脉冲激光波长532nm

Dendronized Carbohydrates Ⅱ——Liquid Crystallinity Study

E-mail: [email protected]; [email protected][中文文摘]以基于单糖为内核,楔形液晶基元DOBOB酸(3,4,5-三[对-(十二烷氧基)苄氧基]苯甲酸)为分枝的树状碳水化合物液晶为研究对象,利用DSC、热台偏光显微镜、XRD和CD/UV光谱等手段研究该类化合物的液晶性.研究发现分枝的数目对该类化合物的液晶性有显著影响,以2-乙酰氨基葡萄糖为内核的包含四个分枝的树状分子具有最高的液晶结构有序性,清亮点也显著高于另两种单糖内核(含五个分枝)的树状分子.此外,该类碳水化合物液晶形成的液晶相都具备超分子手性,为探索碳水化合物手性液晶相提供了一条新的思路.[英文文摘]Liquid crystallinity of dendronized carbohydrate liquid crystals, which contain carbohydrate core and mesogen branches DOBOB (3,4,5-tris(p-dodecyloxybenzyloxy) benzoic acid), was studied by DSC,thermal polarized optical microscopy, XRD and CD/UV spectrum. The number of branches affects the liquid crystallinity significantly. Dendrimer that contains four branches has the highest liquid crystal structure order, and the clearing point is also higher than the other two dendrimers which contain five branches. Otherwise, supramolecular chirality is also expressed in the mesophase formed by these dendronized carbohydrates,which may provide inspiration in searching for chiral mesophase of carbohydrate liquid crystals.国家自然科学基金(No.20774077)资助项

固体无机样品的激光等离子体质谱分析

激光等离子体质谱是一种新颖的质谱分析手段，具有灵敏度高、分析速度快、分析范围广、分析结果准确性高等优点。本文介绍了在自制的激光等离子体源飞行时间质谱计上，有关催化剂、铝箔、活性碳、岩石等固体无机样品的质谱分析结果，显示了激光等离子体质谱的优越性与广泛的应用价值

激光作用产生离子引发的苯乙烯聚合

激光作用产生离子引发的苯乙烯聚合邹友思，林国良，黄荣彬，刘朝阳，郑兰荪（厦门大学化学系固体表面物理化学国家重点实验室，３６１００５）近年来，以高能量密度的脉冲激光束在高真空中溅射固体样品，已成为产生原子簇正负离子的一种有效手段。１９９３年，美国化学家..

C_（60）的产生与石墨晶面的关联（I）

Ｃ_（６０）的产生与石墨晶面的关联（Ｉ）谢兆雄，刘朝阳，林逢辰，王春儒，郑兰荪（厦门大学化学系固体表面物理化学国家重点实验室厦门，３６１００５）关键词Ｃ_（６０），激光溅射，石墨晶面Ｓｍａｌｌａｙ等［１］曾以激光在超声分子束喷口的喉道处蒸发石墨得到了Ｃ..

氯仿至全氯代稠环芳烃的液相电弧合成

以石墨电极在惰性气氛中放电,已成功地合成了C_（60）等碳原子簇。Grosser等人将此反应在氰气与氯气氛中进行,获得了一系列“棒状分子”与全氯代芳烃。我们又将这样的放电反应进一步扩展至溶液体系,将产生原子团簇的物理方法与传统的化学合成方法结合起来。最近,我们分析和研究了在液态的氯仿中放电的产物,在其中先后分离出六氯代苯（Ⅰ）、全

--1H-NMR Spectroscopy-based Metabonomic Research on Serum of Model Rats of Wilson's Disease

目的:以基于核磁共振(nMr)的代谢组学方法对WIlSOn病大鼠模型及正常对照组大鼠血清进行研究,分析血清中小分子代谢物的变化,从小分子代谢物层面上探讨WIlSOn病的内在机制,以更加清楚的认识本病。方法:22只雄性WISTAr大鼠,体重(180±20)g,随机被分为模型组(n=11)和健康对照组(n=11),采用铜负荷法制作WIlSOn病大鼠模型,以核磁共振(nMr)技术对大鼠血清进行检测。采用MESTrE-C 2.3软件及自编软件对谱图进行手动调相、基线校正和谱峰对齐。对样品进行分段积分,将积分数据归一化后构成数据矩阵,并利用PCA方法对数据矩阵进行统计分析。结果:相对于正常对照组,模型组大鼠血清甜菜碱(bETAInE)、氧化三甲胺(TAMO)、低密度脂蛋白(ldl)、极低密度脂蛋白(Vldl)、葡萄糖(gluCOSE)含量有显著降低,胆碱(CHOlInE)、胆碱磷酸(PHOSPHOrylCHOlInE)的含量有所降低,乳酸(lACTATE)、谷氨酰胺(gluTAMInE)、糖蛋白(glyCOPrOTEIn)有显著升高,肌氨酸+肌氨酸酐(CrEATInE+CrEATInInE),精氨酸(ArgInInE)有所升高。这些发生改变的代谢物可以作为Wd的小分子代谢标志物,为进一步研究Wd的内在代谢机制提供参考。Objective:Applying 1H nuclear magnetic resonance(1H-NMR)spectroscopy-based metabonomic approach to investigate the changes of small molecular metabolites in the serum from the rats of the model group of Wilson's disease contrasted with those of the control group.Exploring the pathogenesis of Wilson's disease from small molecular aspect.Methods:22 male Wistar rats[weight=(180±20)g]were divided into two groups randomly,the model group(n=11)and the control group(n=11),with the models established with excessive copper method.The serum was tested with 1H-NMR technology.The spectra were edited with MestRe-C2.3 and self-programmed software and then principal component analysis(PCA)was applied to differentiate the two groups.Results:Choline and phosphorylcholine concentrations were found to be lower and TAMO+betaine,LDL,VLDL and glucose were significantly lower in the serum of the model group.While creatinine and arginine concentrations were found to be higher and lactate,glutamine and glycoprotein were significantly higher in the model group.The small molecular metabolites above may contribute to the discrimination,and serve as references for further research on WD pathogenesis.“十一五”国家科技支撑计划分课题重大疑难疾病中医防治研究项目(2006BA104A02

实时荧光PCR同时检测金黄色葡萄球菌和大肠杆菌O157:H7

目的建立改良分子信标-双重实时荧光PCR同时检测金黄色葡萄球菌和大肠杆菌O157:H7,应用于细菌性食物中毒的快速诊断。方法根据GeneBank公布的金黄色葡萄球菌nuc基因序列和大肠杆菌O157:H7rfbE基因序列,设计引物和改良分子信标探针,建立改良分子信标-双重实时PCR检测体系。结果双重荧光PCR反应体系检测151株金黄色葡萄球菌和27株大肠杆菌O157:H7,均出现特异的荧光信号,两种细菌检测互不干扰。对8762份大便、食品等标本进行检测,315份标本金黄色葡萄球菌实时荧光PCR阳性,其中286份金黄色葡萄球菌培养阳性;31份标本大肠杆菌O157:H7实时荧光PCR阳性,其中26份大肠杆菌O157:H7培养阳性。从样品处理到检测结果仅需要时间2h~1d。结论改良分子信标-多重实时荧光PCR检测体系快速、灵敏度高,特异性强,可用于金黄色葡萄球菌和大肠杆菌O157:H7食物中毒的快速诊断和肠道传染病的初筛,为食源性疾病的分子流行病学调查提供新的检测手段

铝、磷、硫原子簇正负离子的激光产生与质谱研究

以脉冲激光束在高真空下记录第三周期的三个元素—铝、磷、硫原子簇的正负离子的飞行时间质谱。通过对这些质谱的分析研究,列出了在各质谱中出现的“奇幻数”,并讨论了它们可能的结构