Effects of the Xitongxiao prescription on apoptosis and proliferation of chondrocytes in knee

目的：探讨膝痛消方对促进软骨细胞增殖和抑制IL-1β诱导的软骨细胞凋亡的作用。方法：体外培养膝关节软骨细胞，用阿利新蓝染色方法检测软骨细胞蛋白多糖，用Ⅱ型胶原免疫荧光染色检测软骨细胞Ⅱ型胶原的表达，以鉴定软骨细胞；用MTS检测软骨细胞的增殖；用IL-1β诱导软骨细胞凋亡，AnnexinV/PI双染检测细胞凋亡。结果：膝关节剥离得到的细胞形态为多角形或梭形，阿利新蓝与Ⅱ型胶原免疫荧光染色均呈阳性，证明分离培养的细胞为软骨细胞。MTS实验结果发现膝痛消方提取物处理软骨细胞后检测到的OD值随浓度增加而增加且显著高于对照组。此外，IL-1β处理诱导软骨细胞凋亡，而膝痛消方提取物能够有效地抑制IL-1β引起的细胞凋亡。结论：膝痛消方能促进软骨细胞的增殖，抑制IL-1β诱导的软骨细胞的凋亡。以上发现为膝痛消方临床上治疗膝关节骨性关节炎提供药理作用依据。Objective:To investigate the effects of the Xitongxiao prescription on the cell proliferation and the inhibition of IL-1β-induced apoptosis of chondrocytes.Methods:To identify chondrocytes,the articular chondrocytes were cultured in vitro;staining with alcian blue was used to detect the protein polysaccharide,and staining with collagen immunofluorescence was used to examine the expression of type II collagen in chondrocytes.The proliferation of chondrocytes was determined by MTS.Apoptosis of chondrocytes was induced by IL-1β,and the effect of apoptosis was tested by Annexin V/PI double staining.Results:The morphology of the cells obtained from the knee joint was polygonal or fusiform.The tested results of Alcian blue and collagen immunofluorescence staining were positive.These together certified that the isolated cells were knee chondrocytes.MTS assay results showed that the detected OD values were dosedependently increased and they were significantly higher than those of the control group.In addition,the apoptosis of chondrocytes was induced by IL-1βtreatment.While the extract of the Xitongxiao prescription could effectively inhibit the IL-1β-induced apoptosis of chondrocytes.Conclusion:The Xitongxiao prescription could promote the proliferation of chondrocytes and inhibit the apoptosis of chondrocytes induced by IL-1β.The above findings may provide one of pharmacological mechanisms of the Xitongxiao for clinical treatment of knee osteoarthritis.厦门市科技计划项目(3502z20144031);福建省自然科学基金计划资助项目(2015J01065)

Preparation and Application of Soluble Human Squamous Cell Carcinoma Antigen Expressed by Escherichia coli

旨在建立基于大肠杆菌表达系统的高效可溶性表达人鳞状上皮细胞癌抗原(SCCAg)方法,获得具有较好活性的重组SCCAg抗原并应用于建立抗原检测方法; 。基于pGEX-6P-l载体和大肠杆菌E. coli; ER2566菌株开展重组SCCAg抗原可溶性表达纯化方法研究,评价纯化抗原活性,筛选特异性单克隆抗体,初步建立并评价SCCAg抗原检测方法。结果; 显示,pGEX-6P-l载体和E coli; ER2566菌株可用于建立较高效的可溶性表达和纯化SCCAg抗原的方法,获得了具有较高纯度和活性的重组SCCAg抗原,筛选获得特异性单克隆抗体并; 初步建立了 SCCAg管式化学发光检测方法。建立了有效的基于大肠杆菌表达系统的可溶性表达和纯化SCCAg的方法。The aims of this study are to establish a method for efficient soluble; expression of human squamous cell carcinoma antigen(SCCAg ) based on; Escherichia coli expression system and obtain the recombinant SCCAg; antigen in fine activity, then apply it in the detection method; establishment of antigen. The study on the method of soluble expression; and purification of recombinant SCCAg antigen was conducted based on; pGEX-6P-l vector and E. coli ER2566 strain. The activity of the purified; antigen was evaluated by Abbott Kit and the specific monoclonal antibody; was screened by indirect ELISA. It was proved that PGEX-6P-1 vector and; E. coli strain ER2566 could be used to establish efficient soluble; expression and purification method for recombinant SCCAg antigen.; Moreover, the recombinant SCCAg antigen was proved to be in high purity; and activity. Thus,the SCCAg detection method of chemical luminous tube; was established with the specific monoclonal antibodies. In conclusion,; an effective method for the expression and purification of SCCAg, which; is based on the E. coli expression system, is established.国家高技术研究发展计划(863计划

智能光网络基于贝叶斯算法的主动故障告警机制

当前的智能光网络缺乏主动应对网络故障的能力,为了进一步提高智能光网络的生存性,文章提出了一种基于贝叶斯模型的智能光网络主动告警机制。该机制能够预先判断网络中潜在的故障,并具有高度的准确率以有效减少误告警;此外,该机制对不同样本采用不同的算法来进行网络学习,采用概率推理估计故障的预测概率,从而为主动告警机制建立一个可推理的预测模型。仿真结果表明,该机制能极大地提高智能光网络的生存性,缩短业务连接的恢复时延,优化资源利用率

饲草甜高粱分子育种与产业化

甜高粱(Sorghum dochna)是一种高产耐逆的C4植物.因其茎秆富含糖分、生物产量高、适口性好等特点,近年来又被发展为优质饲草作物.甜高粱主要生物学特性直接或间接地影响饲草的产量和品质,进而影响其作为饲草作物的发展潜能.本文综述了甜高粱种质资源筛选与重要性状遗传基础研究的最新进展,分析了甜高粱饲草产业发展案例,旨在积极推动甜高粱饲草产业的研究和应用

Discussion on the Correlation between Land Use Quantitative Structure and its Function at County Level

基于土地利用数量结构与功能特征对应变换关系,选取相关定量指标,以安徽省含山县为例,探讨县域土地利用数量结构与功能特征关联分析方法。结果表明:定量分析具有可行性,操作性强,较为简便。一定程度上,多样性、均匀度景观生态综合功能指标与组合类型数呈同向关联,而优势度与其呈反向关联,经济密度、人口密度社会经济综合功能指标与土地利用程度、建设用地区位指数呈同向关联

一种催化空气氧化醇制备醛和酮的方法

一种催化空气氧化醇制备醛和酮的方法，按100mol的底物醇计， 0.2mol％的2，2，6，6-四甲基哌啶-氧自由基或其衍生物，4-10mol％的活性溴和4-10mol％的亚硝酸盐为催化剂，1-5mL水，100mL二氯甲烷，充空气至压力表为0.4-0.9MPa，100度下反应1-10小时。本发明使用(0.2mol％) 催化量的TEMPO可以将芳香苄醇、杂芳香苄醇(含N，S等元素)、脂肪醇和脂环醇等高选择性地氧化成相应醛和酮。同时，由于使用1，3-二溴-5，5- 二甲基海因作为助催化剂，极大地改善了单质溴素使用所带来的不方便和安全因素。带填

土壤持水特征测定中质量含水量、吸力和容重三者间定量关系I.填装土壤

在试验过程中,通常采用一个土样进行连续试验来测定土壤的持水特征。此时,土样的有机质、黏粒矿物类型和阳离子交换量等性质基本保持不变,而土壤的容重在水力学和机械压力共同作用下会发生很大的变化。因此,我们认为实测的土壤持水特征不再是土壤吸力和含水量相对应的一条曲线,而是由土壤质量含水量、吸力和容重三变量共同确定的一个曲面。本文在Brooks-Corey土壤水分特征曲线模型的基础上,提出了两种描述土壤质量含水量、吸力和容重三变量关系的曲面模型,分析了模型的优缺点和适用条件,并采用离心机石蜡控容重法实测了四种质地填装土壤的三变量特征曲面。研究结果表明:土壤持水特征是由土壤质量含水量、吸力和容重三变量共同确定的一个曲面这一假设是合理的;类似于Brooks-Corey模型的两种幂函数经验曲面模型能够合理地描述填装土壤的实测数据,决定系数均大于0.94;模型Ⅰ的拟合效果略好于模型Ⅱ,但模型Ⅱ包含两个物理意义明确的参数,具有一定的优势。这一研究将为校正容重变化对土壤水力学参数的影响提供新的途径

Exploration of Graduate Teaching Assistants Management

有机化学实验课是培养学生化学基本技能的重要课程。研究生助教实验教学的管理是高校教学管理的一部分。作者根据有机化学实验的特点和助教实践的体会,以保证实验教学质量,提高研究生助教教学水平为着眼点,深入分析了有机化学实验教学中研究生助教工作存在的问题,进而提出相关的解决方案。Abstract: Organic Chemistry Experiment course was an important course. The course aim was to cultivate students’ basic chemistry skills. Graduate assistants management is part of the teaching management in universities. Based on the characteristics of organic chemistry experiment and the experience of teaching assistant，several existed problems in graduate teaching assistant were analyzed and provided some solutions，focusing on the quality of experiment teaching and the teaching level of graduate assistant.广西民族大学2013年度高等教育教学改革工程立项项目(No:2012XJGY44、2012XJGY34、2012XJGY43