9 research outputs found

    活心丸(浓缩丸)治疗冠心病稳定性心绞痛的多中心、随机、双盲、安慰剂对照临床研究

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    目的观察活心丸(浓缩丸)治疗冠心病稳定性心绞痛的临床疗效和安全性。方法本研究采用多中心、随机、双盲、安慰剂对照研究方法,将480例气虚血瘀型冠心病稳定性心绞痛患者以3:1比例随机分为试验组(360例)和对照组(120例)。在西医常规治疗基础上,两组患者分别服用活心丸(浓缩丸)和安慰剂,1次2粒,每日3次;疗程8周。观察两组治疗前后主要指标(心绞痛症状积分)和次要指标(包括硝酸甘油减停率、中医证候积分及生活质量评价)及安全性。结果共454例患者完成试验(试验组336例,对照组118例)。与本组治疗前比较,试验组和对照组心绞痛症状积分和中医证候积分均降低,西雅图心绞痛量表评分改善(P<0.01);与对照组治疗后比较,试验组心绞痛症状积分、中医证候积分、西雅图心绞痛量表评分改善均优于对照组(P<0.01)。试验组心绞痛症状疗效总有效率为80.95%,硝酸甘油停减率为80.70%,中医证候疗效有效率80.65%,均高于对照组[36.44%、45.07%、38.99%(χ~2=58.21、40.94、66.55,P<0.01)]。试验组中既往有心梗史患者心绞痛症状总有效率为83.22%,中医证候有效率83.22%,西雅图心绞痛量表评分为(361.74±62.10)分,均高于无心梗史患者[60.92%、66.89%、(327.95±65.07)分(χ~2=13.89、13.26,P<0.01)]。治疗过程中未发生明显不良反应。结论活心丸(浓缩丸)治疗气虚血瘀型冠心病稳定性心绞痛疗效显著,尤其适用于既往有心梗病史的冠心病稳定性心绞痛患者,无明显不良反应。国家中医药管理局资助课题(No.国中医药科2016ZX04

    新型烟酰胺腺嘌呤二核苷酸(NAD)类似物的合成及其辅酶活性

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    尝试4种形成焦磷酸键的方法合成了5种结构新颖的新型烟酰胺腺嘌呤二核苷酸(NAD)类似物.初步考察了类似物的生物活性,发现苹果酸酶和醇脱氢酶以类似物3b和3d为辅酶时,活性只有以NAD为辅酶时的13%~30%;而以类似物3a,3c和3e为辅酶时,这些酶的活性均极低

    一种NAD+类似物及其合成和应用

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    本发明公开了一种NAD+类似物及其合成和应用,其结构式I,是由烟酰胺单核苷酸与相应的醇反应生成的磷酸二酯化合物。其中R为C4-C15饱和或不饱和的烷基,或含有杂原子的C2-C10饱和或不饱和烷基A;(R见式II)。该NAD+类似物可以促进微生物如大肠杆菌和酿酒酵母的生长;也可作为脱氢酶辅因子用于催化氧化还原反应。带填

    Construction of E. coli NAD+ auxotrophic strains and the biotechnological application thereof

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    NAD+ and its reduced form NADH are essential cofactors in biological systems. They function as cofactors in over 300 redox reactions in vivo1. The level of NAD+ and NADH is tightly controlled by a variety of mechanisms including their biosynthesis and salvage. Therefore, it is difficult to answer some fundamental questions such as the minimal NAD+ level for cell growth and the biological consequences of abnormal activity of a specific NAD+-dependent enzyme. We expressed the NTT4 gene from Chlamydiae UWE25 in Escherichia coli BW25113, as the NTT4 protein was reported as a NAD+ transporter that can specifically transport intact NAD+ across cytoplasmic membrane2. We knocked out the nadC gene responsible for de novo biosynthesis of NAD+ and constructed the strain E. coli BW25113 (ΔnadC, NTT4). It was found that NAD+ in the culture media could significantly promote the growth of BW25113 (ΔnadC, NTT4), suggesting that the NTT4 protein was functional in E. coli (Fig A, B). We then disrupted the other two genes, nadD and nadE, and obtained the strains E. coli BW25113 (ΔnadD, NTT4) and BW25113 (ΔnadE, NTT4). Cell growth of these two strains are depending on exogenous NAD+ supplemented in the media, suggesting that we have successfully engineered E. coli to hold an NAD+ auxotrophic phenotype. We are carrying out a number of experiments using these NAD+ auxotrophic strains to address some interesting questions which may not be able to do otherwise. Results will be discussed during the conference.NAD+ and its reduced form NADH are essential cofactors in biological systems. They function as cofactors in over 300 redox reactions in vivo1. The level of NAD+ and NADH is tightly controlled by a variety of mechanisms including their biosynthesis and salvage. Therefore, it is difficult to answer some fundamental questions such as the minimal NAD+ level for cell growth and the biological consequences of abnormal activity of a specific NAD+-dependent enzyme. We expressed the NTT4 gene from Chlamydiae UWE25 in Escherichia coli BW25113, as the NTT4 protein was reported as a NAD+ transporter that can specifically transport intact NAD+ across cytoplasmic membrane2. We knocked out the nadC gene responsible for de novo biosynthesis of NAD+ and constructed the strain E. coli BW25113 (ΔnadC, NTT4). It was found that NAD+ in the culture media could significantly promote the growth of BW25113 (ΔnadC, NTT4), suggesting that the NTT4 protein was functional in E. coli (Fig A, B). We then disrupted the other two genes, nadD and nadE, and obtained the strains E. coli BW25113 (ΔnadD, NTT4) and BW25113 (ΔnadE, NTT4). Cell growth of these two strains are depending on exogenous NAD+ supplemented in the media, suggesting that we have successfully engineered E. coli to hold an NAD+ auxotrophic phenotype. We are carrying out a number of experiments using these NAD+ auxotrophic strains to address some interesting questions which may not be able to do otherwise. Results will be discussed during the conference

    新疆夏尔希里自然保护区表土孢粉与植被的关系

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    新疆夏尔希里自然保护区保持着较为原始的生态环境,是研究植被与环境变化的理想区域。通过在保护区内从海拔1 042~2 426 m的山地草原化荒漠带、山地干草原带和山地森林带采集的33个表土孢粉样品,结合对每个样点做的植被样方调查,根据孢粉数据进行有序聚类分析和冗余分析,探讨了表土孢粉组合特征与植被之间的对应关系。结果表明:3个孢粉组合带的特征与各垂直带植被总体上有较好的对应;藜科和麻黄属花粉含量与样方植物盖度无明显相关性,这两类孢粉呈现超代表性分布特征,应该是随气流从低海拔地带传播到山地高海拔地带的区域外花粉;桦属花粉和豆科花粉与对应的桦木林及锦鸡儿灌丛植被群落有较好的对应;A/C比值和孢粉总浓度大小在区分森林带与草原化荒漠植被带时有明显的指示意义;蕨类植物孢子与降水量和海拔高度正相关,豆科植物花粉与温度正相关。由于山地地形因素引起的土壤、水分及光照度差异,在相同海拔高度的阳坡与阴坡形成的森林植被和中山草甸植被交替的过渡植被,因此孢粉组合中出现较多的花粉混合,进而降低了云杉和桦属花粉与植被盖度的相关性,这类木本花粉与植被之间的数量关系较为复杂。该现象在植物生态学分析中具有普遍性,但对表土孢粉数据在植被与气候定量重建中的应用具有较大的影响。在运用山地表土孢粉数据进行植被与气候定量重建时,需要结合植被样方资料和沉积环境特征对表土孢粉数据进行校正和筛选

    新显色剂PMNHA的合成、分析鉴定及其在分光光度测定钒中的应用

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    用1-甲基-2-萘甲酰基替换N-苯甲酰苯基羟胺(BPHA)的苯甲酰基,合成了一种新显色剂N-苯基-1-甲氧基-2-萘甲酰氧肟酸(N-phenyl-1-methoxy-2-naphthoylhydroxamicacid,缩写为PMNHA)。根据元素分析、差热分析、质谱分析、核磁共振、红外光谱分析,确定PMNHA的组成和结构为:OCH3CONOHC6H5。该试剂的三氯甲烷溶液能很快从2.25&mdash;4.50mol/LHCl中萃取钒(Ⅴ)的紫色配合物,其配合物最大吸收在540nm,很多外来离子,特别是大量的钛不干扰钒的测定,钒含量在0&mdash;28&mu;g&middot;g-1范围遵守比耳定律。我们建立了一个高选择性和简便的用显色剂PMNHA分光光度测定钒的方

    安西自然保护区生态补偿类比与标准研究

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    一、课题来源与背景 该课题为甘肃省环境保护厅2012年度省环境保护科技计划项目“安西自然保护区生态补偿类比与标准研究”(GSEP-2012-13)。项目背景:近几年来,安西自然保护区内先后有数项国家重点建设工程进行施工或者投产运行,建设前期就工程穿越保护区生态补偿资金事宜,一直是建设者、施工方和保护区三家争议的话题。至目前,国家尚无一部关于自然保护区生态补偿的标准,保护区只能依据甘肃省草原行业生态补偿标准来进行协商补偿,造成补偿资金的争议和资金额度不足以恢复原有生态。项目以西气东输三线工程等已建和在建工程为例,对工程穿越安西自然保护区生态补偿进行定量研究,最终编制《甘肃安西极旱荒漠国家级..
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