Primary study on trans-activation ability of hepatitis B virus envelope proteins

目的构建系列含乙型肝炎病毒(HBV)表面抗原前-前-S区的酵母细胞表达质粒,初步探讨表达蛋白是否具有反式激活作用。方法用多聚酶链反应(PCR)扩增含前-前-S区的全S、S2和S基因,定向克隆于pDEST32载体,进行序列测定,重组质粒命名为pDEST32-wS、pDEST32-pS2和pDEST32-SHBs。用乙酸锂转化法将重组质粒转化酵母菌MaV203,经Western blot和胶体金法验证重组质粒在酵母细胞中的表达。以酵母双杂交法将重组质粒和载体pDEST22共同转入酵母菌MaV203,并在SC/-leu/-trp/-his三缺培养基及不同浓度梯度3AT培养基中验证自激活。结果重组质粒pDEST32-wS经序列测定含有HBV自前-前-S至主蛋白基因序列。经Western blot和胶体金法证实转染的酵母细胞可表达表面抗原蛋白。pDEST32-wS与pDEST22共转染实验证实被转染酵母细胞不能在浓度30 mmol/L以上的3AT培养基生存。结论构建了pDEST32-wS、pDEST32-pS2和pDEST32-SHBs表达载体,pDEST32-wS和pDEST32-SHBs在酵母细胞中可表达HBsAg... 【英文摘要】 Objective To construct yeast expression vector of HBV whole-S gene including pre-pre-S region and explore the trans-activation function of whole-S protein.Methods Whole-S gene,pre-pre-S to pre-S2 region and major HBsAg coding region containing Not I and Sal I endoenzyme sites were obtained by PCR method.After enzyme digestion,PCR products were cloned into yeast expression vector pDEST32.Recombinant plasmids were sequenced and named as pDEST32-wS,pDEST32-pS2 and pDEST32-SHBs,respectively.Recombinant plasmids...厦门市首批重大疾病科研攻关项目(WKZ0501);; 厦门市卫生局医学科研立项项目(WSK0506);; 厦门大学引进人才科研启动基金(Z03109);; 福建省青年科技人才创新项目(2006F3127);; 福建省青年科技人才创新项目(2006F3127

ON STATISTICAL APPROXIMATION PROPERTIES OF MODIFIED q-BERNSTEIN-SCHURER OPERATORS

National Natural Science Foundation of China [61170324]; Education Department of Fujian Province of China [JA12324]; Natural Science Foundation of Fujian Province of China [2010J01012]In this paper, a kind of modified q-Bernstein-Schurer operators is introduced. The Korovkin type statistical approximation property of these operators is investigated. Then the rates of statistical convergence of these operators are also studied by means of modulus of continuity and the help of functions of the Lipschitz class. Furthermore, a Voronovskaja type result for these operators is given

Approximation by complex q-Bernstein-Schurer operators in compact disks

National Natural Science Foundation of China [61170324]; Class A Science and Technology Project of Education Department of Fujian Province of China [JA12324]; Natural Science Foundation of Fujian Province of China [2010J01012]In this paper we introduce a class of complex q-Bernstein-Schurer operators and study the approximation properties of these operators. We obtain the order of simultaneous approximation and a Voronovskaja-type result with a quantitative estimate for these complex q-Bernstein-Schurer operators attached to analytic functions in compact disks. More important, our results show the overconvergence phenomenon for these complex operators

King type modification of q-Bernstein-Schurer operators

National Natural Science Foundation of China [61170324]; Class A Science and Technology Project of Education Department of Fujian Province of China [JA12324]; Natural Science Foundation of Fujian Province of China [2010J01012]Very recently the q-Bernstein-Schurer operators which reproduce only constant function were introduced and studied by C. V. Muraru (2011). Inspired by J. P. King, Positive linear operators which preserve x (2) (2003), in this paper we modify q-Bernstein-Schurer operators to King type modification of q-Bernstein-Schurer operators, so that these operators reproduce constant as well as quadratic test functions x (2) and study the approximation properties of these operators. We establish a convergence theorem of Korovkin type. We also get some estimations for the rate of convergence of these operators by using modulus of continuity. Furthermore, we give a Voronovskaja-type asymptotic formula for these operators

Some statistical approximation properties of Kantorovich-type q-Bernstein-Stancu operators

National Natural Science Foundation of China [61170324]; Class A Science and Technology Project of Education Department of Fujian Province of China [JA12324]; Natural Science Foundation of Fujian Province of China [2013J01017]In this paper two kinds of Kantorovich-type q-Bernstein-Stancu operators are introduced, and the statistical approximation properties of these operators are investigated. Furthermore, by means of modulus of continuity, the rates of statistical convergence of these operators are also studied

活性炭によるかつお節加工残滓熱水抽出液中の多環芳香族炭化水素類（pahs）の除去

かつお節加工残滓を安全かつ有効に利用する研究の一環として，まず加工残滓の熱水抽出液に PAHs を合計 3217 μg/L 添加したモデル試料の活性炭による PAHs 最適除去条件を有用成分への影響も考慮しつつ検討した。その結果，PAHs 除去率は最大 99.6% であったが，活性炭の種類による影響が大きかった。続いて，市販品の黒粉 2 種を熱水，エタノール，酢酸エチルで抽出し，PAHs 浸出量を分析した。エタノール抽出で PAHs が最大 12823 μg/L 浸出し，市販品にも高濃度の PAHs が含まれていることが分かった。As part of our ongoing study for safely and eŠectively using dried bonito processing residue while considering its useful components, we investigated the optimal conditions for removal of polycyclic aromatic hydrocarbons (PAHs) from dried bonito processing residue. First, as model sample, we added 3217 mg/L of PAHs to a hot water extract of processing residues to verify the PAH removal rate with activated carbon. The maximum PAH removal rate was 99.6, and the PAH removal rate was found to be dependent on the type of activated carbon.We then performed PAH extraction on two commercially available ``kuroko\u27\u27 (dried bonito shavings powder) products using hot water, ethanol, and ethyl acetate, and analyzed the PAH amount in each extract. The highest amount of PAHs (12823 mg/L) was detected in the ethanol extract, revealing that commercial products contain highly concentrated PAHs