类泛素蛋白及其中文命名

泛素家族包括泛素及类泛素蛋白,约20种成员蛋白.近年来,泛素家族领域取得了迅猛发展,并已与生物学及医学研究的各个领域相互交叉.泛素家族介导的蛋白质降解和细胞自噬机制的发现分别于2004和2016年获得诺贝尔奖.但是,类泛素蛋白并没有统一规范的中文译名. 2018年4月9日在苏州召开的《泛素家族介导的蛋白质降解和细胞自噬》专著的编委会上,部分作者讨论了类泛素蛋白的中文命名问题,并在随后的\"泛素家族、自噬与疾病\"(Ubiquitinfamily,autophagy anddiseases)苏州会议上提出了类泛素蛋白中文翻译草案,此草案在参加该会议的国内学者及海外华人学者间取得了高度共识.冷泉港亚洲\"泛素家族、自噬与疾病\"苏州会议是由美国冷泉港实验室主办、两年一度、面向全球的英文会议.该会议在海内外华人学者中具有广泛影响,因此,参会华人学者的意见具有一定的代表性.本文介绍了10个类别的类泛素蛋白的中文命名,系统总结了它们的结构特点,并比较了参与各种类泛素化修饰的酶和它们的生物学功能.文章由45名从事该领域研究的专家合作撰写,其中包括中国工程院院士1名,相关学者4名,长江学者3名,国家杰出青年科学基金获得者18名和美国知名高校华人教授4名.他们绝大多数是参加编写即将由科学出版社出版的专著《泛素家族介导的蛋白质降解和细胞自噬》的专家

归属需要受挫和自我控制在大学生家庭功能与错失焦虑之间的链式中介作用

目的探讨大学生家庭功能与错失焦虑之间的关系及归属需要受挫与自我控制在其中的链式中介作用。方法采用家庭功能量表、归属需要满足与受挫量表、自我控制量表和错失焦虑量表, 对广东省五所高校409名大学生进行问卷调查。运用SPSS 26.0进行共同方法偏差检验、Pearson相关分析和分层回归分析, 采用PROCESS 3.5宏程序进行中介效应检验。结果 (1)家庭功能、归属需要受挫、自我控制和错失焦虑的总分分别为(166.01±16.08)分、(9.39±2.51)分、(37.88±6.27)分、(56.38±6.59)分。(2)家庭功能与归属需要受挫呈显著负相关(r =-0.43, P<0.01), 与自我控制呈显著正相关(r =0.43, P<0.01), 与错失焦虑呈显著负相关(r =-0.39, P<0.01);归属需要受挫与自我控制呈显著负相关(r =-0.37, P<0.01), 与错失焦虑呈显著正相关(r =0.38, P<0.01);自我控制与错失焦虑呈显著负相关(r =-0.42, P<0.01)。(3)家庭功能对错失焦虑的直接效应值为-0.17(95%CI =-0.27～-0.07), 家庭功能通过三条路径间接影响错失焦虑, 归属需要受挫的单独中介作用效应值为-0.09(95%CI =-0.14～-0.05), 自我控制的单独中介作用效应值为-0.09(95%CI =-0.14～-0.05), 归属需要受挫和自我控制的链式中介作用效应值为-0.03(95%CI =-0.04～-0.01)。结论家庭功能不仅可以直接影响错失焦虑, 也可以通过归属需要受挫和自我控制的链式中介作用间接影响错失焦虑

Subcellular Localization of Estrogen Receptor α and Its Relationship with Cell Proliferation

雌激素受体(Estrogen Receptor,ER)是核受体(Nucleus Receptor,NR)超家族成员,能够被其配体雌二醇(Steroidhormone 17-β-estradiol,E2)激活.ER有两种亚型,ERα和ERβ,由4个不同的功能域组成.通过构建ERα的不同缺失突变体(ERΔLBD、ERΔDBD和ERΔHinge)来研究不同功能域对细胞增殖及在E2刺激下对ERα亚细胞定位的影响.结果表明,ERα呈核多浆少的分布,ERΔLBD为核定位,ERΔDBD为核多浆少分布,ERΔHinge则主要分布在胞浆.进一步分析表明,E2诱导的ERα入核是核定位序列(Nuclear Location Signal,NLS)依赖性.此外,我们还发现,ERα能够增强293T细胞增殖,且这种促增殖效应可能依赖于ERα在细胞核与胞浆的正常转运. 【英文摘要】 Estrogen receptor(ER),known as a hormone receptor,is one of the members of nucleus receptor(NR).ER has been shown to be activated in response to its ligand,steroid hormone 17-β-estradiol(E2).There are two kinds of ER,ERα and ERβ.ERs have four different functional regions,including transcriptional activity domain,ligand binding domain,hinge domain and DNA binding domain.In this study,three different ERα mutants(ERΔLBD、ERΔDBD and ERΔHinge) were constructed to study the influence of ERα in cell proliferation a...福建省自然科学基金(C0410003)资

基于蒙特卡洛方法的摆线针轮接触刚度研究

为了更加准确地计算出摆线针轮的接触刚度，更好地指导实际应用，利用蒙特卡洛方法对其进行分析，提出了一种基于蒙特卡洛方法的摆线针轮接触刚度计算方法。该方法分别建立了摆线轮齿轮齿廓修形前和摆线轮齿廓齿廓修形后的齿廓函数，探究了齿轮修形对摆线针轮接触刚度的影响，最后得到了较为准确的摆线针轮接触刚度的表达式。以工业机器人RV-40E减速器为例，通过计算实例表明，针对摆线轮的齿轮齿廓修形会改变摆线轮的接触刚度，摆线针轮接触刚度随着修形量的增加先减小后增大再减小

茂县花椒化学成分分析及抑菌活性研究

综合运用多种化学分析方法对茂县花椒化学成分进行深入研究,并探索其抑菌活性,结果表明:样品水分含量9.5%,总灰分含量5.5%,总碳含量44.14%,总氮含量2.23%,碳氮比19.79,铵态氮含量13.56mg/kg,蛋白质含量9.72g/100g;经氨基酸自动分析仪检测其含有17种氨基酸,总量达到7.15%;经红外光谱、电子鼻和GC-MS定性与定量分析,其挥发性成分主要包含酯类、醛类、烯类、醇类、酮类等。抑菌活性研究表明:其对枯草芽孢杆菌和金黄色葡萄球菌等阳性菌具有明显的抑制作用,对大肠杆菌和乙型副伤寒沙门氏菌等阴性菌的抑菌活性不明显。研究较全面地分析了茂县花椒的化学基础和生物活性,为其进一步开发应用奠定一定的理论基础

Trend analysis of differentially expressed genes in gill of Exopalaemon carinicauda under hypoxia-reoxygenation stress

【Objective】To investigate the molecular regulation mechanisms in gill tissue of Exopalaemon carinicauda under gradually changing hypoxia-reoxygenation stress,so as to provide theoretical reference for the breeding of hypoxia tolerance strains(species)of E. carinicauda.【Method】By simulating the formation process of hypoxia in the natural environment, gill tissues were collected at 0(control),3 and 6 h,and 1 and 8 h after reoxygenation,respectively. Transcriptome sequencing analysis was performed using Illumina HiSeqTM 4000 sequencing platform. Unigenes were obtained by filtration and Trinity assembly. Nr,Swiss-Prot,KEGG and COG/KOG databases were selected for annotation analysis. The differentially expressed genes(DEGs)were screened and their trends were analyzed on the Omicsmart platform. Then GO functional annotation analysis and KEGG signal pathway enrichment analysis were performed,and five DEGs were randomly selected for real-time quantitative PCR(qRT-PCR)validation.【Result】A total of 93227 uni genes whose range were from 201 to 35402 bp were obtained through transcriptome sequencing,with their average length of 834 bp and the N50 of 1352 bp. 4750 genes were identified as DEGs through pairwise comparison between groups,of which the up-regulated genes were 3557 and the down-regulated genes were 2829. More than fifty percent were significantly enriched in six gene expression trends(P<0.01):Profile 0 was enriched in 415 genes;Profile 5 was enriched in 201 genes; Profile 11 was enriched in 371 genes;Profile 13 was enriched in 841 genes;Profile 17 was enriched in 387 genes and Profile 18 was enriched in 411 genes.The DEGs in six trends were annotated to metabolic processes,cellular processes,single organism process,cell,cell parts,macromolecular complex and catalytic activity by GO functional analysis. The KEGG signaling pathway enrichment analysis showed that DEGs in Profile 13 were enriched in 86 pathways at most and significantly enriched in ribosome,carbon metabolism,oxidative phosphorylation,biosynthesis of amino acids,protein processing in endoplasmic reticulum,glycolysis or gluconeogenesis,glutathione metabolism and protein export.【Conclusion 】The gill tissues of E.carinicauda synthesizes protein and increases metabolic capacity to resist hypoxia in the early stage of hypoxia stress. But with the prolongation of hypoxia,both substance synthesis and energy metabolism were affected and decreased. After reoxygenation,with the prolongation ofreoxygenation,the protein synthesis and energy metabolism gradually recover to level under normal oxygen