Education Quality Improvement by E-learning Technology

The purpose of this article is to describe the current state and current needs, as well as efforts put in implementation of information and communication technology (ICT) into Croatian higher education practice. In order to do so, the article will cover following topics: ICT in education - e-learning, What is e-learning, Features, avantages, disadvantages of e-learning, E-learning delivery, Implementatio strategic planning, n of e-learning: "Lone Ranger" model or insitutional strategical planning, EQIBELT project - how to institutinally support e-learning implementation into Croatian higher education practice

Education Quality Improvement by E-learning Technology

The purpose of this article is to describe the current state and current needs, as well as efforts put in implementation of information and communication technology (ICT) into Croatian higher education practice. In order to do so, the article will cover following topics: ICT in education - e-learning, What is e-learning, Features, avantages, disadvantages of e-learning, E-learning delivery, Implementatio strategic planning, n of e-learning: "Lone Ranger" model or insitutional strategical planning, EQIBELT project - how to institutinally support e-learning implementation into Croatian higher education practice

Institutional Support for E-learning Implementation in Higher Education Practice: a Case Report of University of Rijeka, Croatia

E-learning implementation in the teaching and learning process has been presented as an institutional, strategically planned operation at University of Rijeka, Croatia. Motivated by poor use of ICT in teaching and learning process, together with the Bologna process changes needed in transformation of curricula, as well as by participation in one Tempus project on e-learning implementation in higher education, the University management decided to facilitate the activities for e-learning implementation with supporting policy documents as well as through allocating budget. The implementation started practically from ground zero, with the aim of building capacities - setting up the infrastructure and environment ready for the implementation, as well as building a community of practitioners. The three-year long process has resulted with achievements that exceeded the expectations

Oxidation-induced Spin Probes in Low-density Lipoproteins

PBN trapping indicated the presence of at least two types of radicals induced in LDL upon slow oxidation, as observed with EPR spectroscopy. The EPR spectra are very similar to those of the spin probes or spin labels, which are routinely used in the studies of macromolecular systems and supramolecular assemblies. The parameters of the spectral line shapes, treated in two different theoretical models, did not provide conclusive evidence for the phase transition in the LDL particles, known to be present at about 25 °C. It is concluded that both types of trapped radicals, or at least one of them, are associated with the apolipoproteins, with the spin probe sticking into the lipid domain of phospholipids

Recent results from computer simulations of membrane channels and pumps

The present review reports on recent progress in modeling and simulation of biological ion channels and proton pumps. In particular, we describe the current approaches and results concerning permeation and selectivity of ion channels. This is demonstrated mainly at the example of a potassium ion channel. In the case of proton pumping across lipid membranes we report on recent results from computer simulation of bacteriorhodopsin. The distribution of internal water and the structure and dynamics of hydrogen-bonded networks inside bacteriorhodopsin are discussed

Strong nuclear EGFR expression in colorectal carcinomas is associated with cyclin-D1 but not with gene EGFR amplification

<p>Abstract</p> <p>Background</p> <p>Prognostic and predictive significance of epidermal growth factor receptor (EGFR) in colorectal carcinomas (CRCs) is still controversial. The aim of the present study was to explore and correlate membrane and nuclear EGFR and cyclin-D1 protein expression with EGFR gene status of tumor cells.</p> <p>Methods</p> <p>Immunohistochemical and FISH analysis was performed on 135 archival formalin fixed and paraffin embedded CRCs.</p> <p>Results</p> <p>Strong membrane and strong nuclear EGFR staining was detected in 16% and 57% of cases, respectively, and strong cyclin-D1 expression in 57% samples. Gene EGFR amplification was identified in 5.9% and polysomy in 7.4% of cases, while 87% showed no EGFR gene changes. A statistically significant difference was only found between tumor grade and expression of membrane EGFR, while nuclear EGFR and cyclin-D1 expression was not associated with the clinicopathologic characteristics analyzed. Tumor cells displaying gene amplification and strong protein membrane EGFR expression overlapped, while EGFR gene status showed no correlation with nuclear EGFR and cyclin-D1. There was no association between membrane EGFR and cyclin-D1, whereas nuclear EGFR expression was strongly related to cyclin-D1 expression.</p> <p>Conclusions</p> <p>Study results revealed heterogeneity among CRCs, which could have a predictive value by identifying biologically and probably clinically different subsets of tumors with the possibly diverse response to anti-EGFR therapies.</p

Potential Value of Hepatic Lipids from White Sea Bream (Diplodus sargus, L.) as a Good Source of Biomedical Components: Seasonal Variations

Svrha je ovoga istraživanja bila ispitati potencijalnu važnost lipida jetre šaraga (Diplodus sargus L.) kao izvora značajnih biomedicinskih komponenata. Određen je sastav masnih kiselina u nepolarnim (triacilgliceroli) i polarnim (fosfatidilinozitol, fosfatidilserin, fosfatidilkolin i fosfatidiletanolamin) lipidnim frakcijama jetre. Da bi se ispitalo kako godišnje doba utječe na sastav masnih kiselina lipidnih frakcija jetre, ribe su lovili i analizirali tijekom zime, proljeća, ljeta i jeseni. Identificirano je ukupno 18 različitih masnih kiselina u analiziranim uzorcima. Među zasićenim masnim kiselinama najzastupljenije su bile palmitinska (16:0) i stearinska (18:0), među mononezasićenima oleinska (18:1 n-9) i palmitoleinska (16:1 n-7), a arahidonska (20:4 n-6), eikozapentaenska (20:5 n-3) i dokozaheksaenska kiselina (22:6 n-3) među polinezasićenim masnim kiselinama. Njihovi su se udjeli u različitim frakcijama lipida bitno razlikovali tijekom pojedinog godišnjeg doba. Ukupni udjel nezasićenih masnih kiselina u svim analiziranim frakcijama lipida bio je najveći u zimskom razdoblju. U proljeće su zasićene masne kiseline dostigle najviše vrijednosti u svim lipidnim frakcijama. Najveći je udjel eikozapentaenske i dokozaheksaenske kiseline utvrđen u triacilglicerolima u zimskom razdoblju. Ustanovljeno je da godišnje doba utječe i na indekse nezasićenosti i n-3/n-6 omjere. Rezultati ovog istraživanja pokazuju ovisnost sastava masnih kiselina u lipidima jetre D. sargus o godišnjem dobu.The aim of this study is to evaluate the potential value of hepatic lipids of the white sea bream, Diplodus sargus, as a source of important biomedical components. Fatty acid compositions of hepatic neutral (triacylglycerols) and polar (phosphatidylinositol, phosphatidylserine, phosphatidylcholine, or phosphatidylethanolamine) lipid fractions were determined. In order to verify the influence of a season on the fatty acid compositions of liver lipid fractions, fish were captured and analyzed in winter, spring, summer and autumn. Eighteen different fatty acids were identified in the analyzed lipid fractions. The major constituents of total fatty acids were saturates: palmitic (16:0) and stearic acid (18:0), monounsaturated fatty acids: oleic (18:1 n-9) and palmitoleic acid (16:1 n-7), while arachidonic acid (20:4 n-6), eicosapentaenoic (20:5 n-3) and docosahexaenoic acid (22:6 n-3) were the major constituents among polyunsaturated fatty acids. Their amounts and ratios differed significantly among seasons in different lipid fractions. Total unsaturated fatty acids in all analyzed lipid fractions were the highest in the winter period. Saturated fatty acids were the highest in the spring period in all lipid fractions. Eicosapentaenoic and docosahexaenoic acid achieved the highest values in triacylglycerols in the winter period. Unsaturation indices and n-3/n-6 values were also influenced by the season. This study revealed a seasonality pattern of D. sargus hepatic fatty acid composition

Istraživanje važnosti lipida jetre šaraga (Diplodus sargus L.), kao izvora biomedicinskih komponenata, ovisno o godišnjem dobu

The aim of this study is to evaluate the potential value of hepatic lipids of the white sea bream, Diplodus sargus, as a source of important biomedical components. Fatty acid compositions of hepatic neutral (triacylglycerols) and polar (phosphatidylinositol, phosphatidylserine, phosphatidylcholine, or phosphatidylethanolamine) lipid fractions were determined. In order to verify the influence of a season on the fatty acid compositions of liver lipid fractions, fish were captured and analyzed in winter, spring, summer and autumn. Eighteen different fatty acids were identified in the analyzed lipid fractions. The major constituents of total fatty acids were saturates: palmitic (16:0) and stearic acid (18:0), monounsaturated fatty acids: oleic (18:1 n-9) and palmitoleic acid (16:1 n-7), while arachidonic acid (20:4 n-6), eicosapentaenoic (20:5 n-3) and docosahexaenoic acid (22:6 n-3) were the major constituents among polyunsaturated fatty acids. Their amounts and ratios differed significantly among seasons in different lipid fractions. Total unsaturated fatty acids in all analyzed lipid fractions were the highest in the winter period. Saturated fatty acids were the highest in the spring period in all lipid fractions. Eicosapentaenoic and docosahexaenoic acid achieved the highest values in triacylglycerols in the winter period. Unsaturation indices and n-3/n-6 values were also influenced by the season. This study revealed a seasonality pattern of D. sargus hepatic fatty acid composition.Svrha je ovoga istraživanja bila ispitati potencijalnu važnost lipida jetre šaraga (Diplodus sargus L.) kao izvora značajnih biomedicinskih komponenata. Određen je sastav masnih kiselina u nepolarnim (triacilgliceroli) i polarnim (fosfatidilinozitol, fosfatidilserin, fosfatidilkolin i fosfatidiletanolamin) lipidnim frakcijama jetre. Da bi se ispitalo kako godišnje doba utječe na sastav masnih kiselina lipidnih frakcija jetre, ribe su lovili i analizirali tijekom zime, proljeća, ljeta i jeseni. Identificirano je ukupno 18 različitih masnih kiselina u analiziranim uzorcima. Među zasićenim masnim kiselinama najzastupljenije su bile palmitinska (16:0) i stearinska (18:0), među mononezasićenima oleinska (18:1 n-9) i palmitoleinska (16:1 n-7), a arahidonska (20:4 n-6), eikozapentaenska (20:5 n-3) i dokozaheksaenska kiselina (22:6 n-3) među polinezasićenim masnim kiselinama. Njihovi su se udjeli u različitim frakcijama lipida bitno razlikovali tijekom pojedinog godišnjeg doba. Ukupni udjel nezasićenih masnih kiselina u svim analiziranim frakcijama lipida bio je najveći u zimskom razdoblju. U proljeće su zasićene masne kiseline dostigle najviše vrijednosti u svim lipidnim frakcijama. Najveći je udjel eikozapentaenske i dokozaheksaenske kiseline utvrđen u triacilglicerolima u zimskom razdoblju. Ustanovljeno je da godišnje doba utječe i na indekse nezasićenosti i n-3/n-6 omjere. Rezultati ovog istraživanja pokazuju ovisnost sastava masnih kiselina u lipidima jetre D. sargus o godišnjem dobu

Potential Value of Hepatic Lipids from White Sea Bream (Diplodus sargus, L.) as a Good Source of Biomedical Components: Seasonal Variations

The aim of this study is to evaluate the potential value of hepatic lipids of the white sea bream, Diplodus sargus, as a source of important biomedical components. Fatty acid compositions of hepatic neutral (triacylglycerols) and polar (phosphatidylinositol, phosphatidylserine, phosphatidylcholine, or phosphatidylethanolamine) lipid fractions were determined. In order to verify the influence of a season on the fatty acid compositions of liver lipid fractions, fish were captured and analyzed in winter, spring, summer and autumn. Eighteen different fatty acids were identified in the analyzed lipid fractions. The major constituents of total fatty acids were saturates: palmitic (16:0) and stearic acid (18:0), monounsaturated fatty acids: oleic (18:1 n-9) and palmitoleic acid (16:1 n-7), while arachidonic acid (20:4 n-6), eicosapentaenoic (20:5 n-3) and docosahexaenoic acid (22:6 n-3) were the major constituents among polyunsaturated fatty acids. Their amounts and ratios differed significantly among seasons in different lipid fractions. Total unsaturated fatty acids in all analyzed lipid fractions were the highest in the winter period. Saturated fatty acids were the highest in the spring period in all lipid fractions. Eicosapentaenoic and docosahexaenoic acid achieved the highest values in triacylglycerols in the winter period. Unsaturation indices and n-3/n-6 values were also influenced by the season. This study revealed a seasonality pattern of D. sargus hepatic fatty acid composition

Potential Value of Hepatic Lipids from White Sea Bream (Diplodus sargus, L.) as a Good Source of Biomedical Components: Seasonal Variations

Svrha je ovoga istraživanja bila ispitati potencijalnu važnost lipida jetre šaraga (Diplodus sargus L.) kao izvora značajnih biomedicinskih komponenata. Određen je sastav masnih kiselina u nepolarnim (triacilgliceroli) i polarnim (fosfatidilinozitol, fosfatidilserin, fosfatidilkolin i fosfatidiletanolamin) lipidnim frakcijama jetre. Da bi se ispitalo kako godišnje doba utječe na sastav masnih kiselina lipidnih frakcija jetre, ribe su lovili i analizirali tijekom zime, proljeća, ljeta i jeseni. Identificirano je ukupno 18 različitih masnih kiselina u analiziranim uzorcima. Među zasićenim masnim kiselinama najzastupljenije su bile palmitinska (16:0) i stearinska (18:0), među mononezasićenima oleinska (18:1 n-9) i palmitoleinska (16:1 n-7), a arahidonska (20:4 n-6), eikozapentaenska (20:5 n-3) i dokozaheksaenska kiselina (22:6 n-3) među polinezasićenim masnim kiselinama. Njihovi su se udjeli u različitim frakcijama lipida bitno razlikovali tijekom pojedinog godišnjeg doba. Ukupni udjel nezasićenih masnih kiselina u svim analiziranim frakcijama lipida bio je najveći u zimskom razdoblju. U proljeće su zasićene masne kiseline dostigle najviše vrijednosti u svim lipidnim frakcijama. Najveći je udjel eikozapentaenske i dokozaheksaenske kiseline utvrđen u triacilglicerolima u zimskom razdoblju. Ustanovljeno je da godišnje doba utječe i na indekse nezasićenosti i n-3/n-6 omjere. Rezultati ovog istraživanja pokazuju ovisnost sastava masnih kiselina u lipidima jetre D. sargus o godišnjem dobu.The aim of this study is to evaluate the potential value of hepatic lipids of the white sea bream, Diplodus sargus, as a source of important biomedical components. Fatty acid compositions of hepatic neutral (triacylglycerols) and polar (phosphatidylinositol, phosphatidylserine, phosphatidylcholine, or phosphatidylethanolamine) lipid fractions were determined. In order to verify the influence of a season on the fatty acid compositions of liver lipid fractions, fish were captured and analyzed in winter, spring, summer and autumn. Eighteen different fatty acids were identified in the analyzed lipid fractions. The major constituents of total fatty acids were saturates: palmitic (16:0) and stearic acid (18:0), monounsaturated fatty acids: oleic (18:1 n-9) and palmitoleic acid (16:1 n-7), while arachidonic acid (20:4 n-6), eicosapentaenoic (20:5 n-3) and docosahexaenoic acid (22:6 n-3) were the major constituents among polyunsaturated fatty acids. Their amounts and ratios differed significantly among seasons in different lipid fractions. Total unsaturated fatty acids in all analyzed lipid fractions were the highest in the winter period. Saturated fatty acids were the highest in the spring period in all lipid fractions. Eicosapentaenoic and docosahexaenoic acid achieved the highest values in triacylglycerols in the winter period. Unsaturation indices and n-3/n-6 values were also influenced by the season. This study revealed a seasonality pattern of D. sargus hepatic fatty acid composition