Efekat programa ishrane na pojavu lezija na nogama i produktivnost brojlera

Nutrition factors are considered to be very important for incidence of foot-pad dermatitis through the effect on the quality of litter. Objective of this study was to investigate the effect of the use of two feeding programs for broilers available on the market and declared in the nutritional/economical sense as standard and economical, on quality of litter, incidence of leg dermatitis (foot-pad and hock burns) and production performance. Trial was carried out on 500 one day old chickens of Hubbard genotype, in two treatments and five replicates. Chopped straw was used as litter. During the trial, the mortality and food consumption were monitored, and at the end of trial the body mass was controlled, as well as the incidence of foot-pad and hock burns and their severity were evaluated and quality of litter analyzed. Research results indicate significant effect of the diets from the aspect of broiler welfare and productivity. Application of the feeding program declared in the nutritional/economical sense as economical resulted in higher content of litter moisture, significantly higher frequency of incidence of the most severe forms of foot-pad dermatitis and significantly lower broiler performance.Ishranbeni faktori se smatraju veoma značajnim za nastanak dermatitisa na nožnim jastučićima brojlera preko uticaja na kvalitet prostirke. Cilj rada je bio ispitivanje efekta upotrebe dva, tržišno dostupna programa ishrane brojlera koji su deklarisani u nutritivno/ekonomskom smislu kao standardan i ekonomičan, na kvalitet prostirke, pojavu dermatitisa na nogama i proizvodne performanse. Ogled je izveden na 500 jednodnevnih pilića genotipa Hubbard, sa dva tretmana i pet ponavljanja. Za prostirku je korišćena seckana slama. Tokom ogleda su evidentirani mortalitet i utrošak hrane a na kraju ogleda je izvršena kontrola telesne mase, ocena pojave i težine oblika lezija na nožnim jastučićima i skočnim zglobovima, analiza kvaliteta prostirke. Rezultati istraživanja su ukazali na značajan efekat programa ishrane sa stanovišta dobrobiti i produktivnosti brojlera. Primena programa ishrane deklarisanog u nutritivno/ekonomskom smislu kao ekonomičnog, rezultirala je većim sadržajem vlage u prostirci, značajno većom frekvencijom pojavljivanja najtežih oblika dermatitisa na nožnim jastučićima i značajno lošijim performansama brojlera

Dermatitis tabanskih jastučića kod komercijalnih brojlera

In the present experiment, the presence of mycological agents in chickens with skin lesions on foot pads was investigated, in order to improve the health of broiler chickens. Foot-pad dermatitis (FPD) is described in the literature as dermatitis caused by multifactor causes. The presence of irritating chemical substances in the litter, genetic predispositions of hybrids, immune suppressive diseases of chickens, as well as the composition of commercial broiler diets that influence the occurrence of wet litter, are proven factors that contribute to a more intensive incidence of foot-pad dermatitis. Foot-pad dermatitis is a condition characterized by lesions on metatarsal and digital ventral skin of poultry feet. It is type of contact dermatitis which in severe cases shows slower weight gain, poor health condition and welfare. Taking into consideration the literature data, chickens reared in closed facilities where the bacterial and mycological agents are present partly in the litter if not properly disinfected, but also in the food or the chicken plumage, we wanted to investigate their presence in the skin of foot pads. The study was focused on incidence of foot-pad dermatitis in 500 one day old broiler chickens of Hubbard genotype, in two feeding (standard/economical) programs for broilers available on our market. Chopped straw was used as litter. At the end of fattening, all chickens were examined for the presence of lesions on the skin of the feet. Total of 39 chickens were allocated to the intensity of the lesion on foot pads rated as 2 (mild) and 3 (severe). In the mycological examination of the 9 samples of autoclaved chicken legs with lesions on the feet, we have isolated and identified the presence of fungi /mould Aspergillus fumigatus. The histopathology investigation, during which the tissue section from mild and severe skin foot pads was collected and stained using Hematoxylin and eosin, revealed the hyperkeratosis, panniculitis, ballooning degeneration and dermis inflammation. No fungal elements were observed in the sections of pads stained with Gomori methenamine silver. Birds are especially susceptible to infection with Aspergillus fumigatus. That species of fungus is also classified as allergenic factor.U ogledu je ispitivano prisustvo mikoloških agenasa kod pilića sa lezijama na koži tabanskih jastučića, u cilju poboljšanja zdravlja brojlerskih pilica. Dermatitis na tabanskim jastučićima (DTJ) je u literaturi multifaktornog porekla. Postojanje iritantnih hemijskih supstanci u prostirci, genetske predispozicije hibrida, imunosupresivne bolesti pilića, kao i sastav komercijalnih smeša za ishranu brojlera koje utiču na pojavu vlažne prostirke, su dokazani faktori koji doprinose intenzivnijoj pojavi DTJ. Imajući u vidu ove podatke iz literature, pilići koji se gaje u zatvorenom prostoru u kome su prisutni bakterijski i mikološki agensi delom u prostirci ako nije na adekvatan način dezinfikovana, u hrani ili na perju pilića, želeli smo da ispitamo njihovo prisustvo u koži tabanskih jastučića. Ispitivanje je bazirano na pojavi DTJ u ogledu 500 jednodnevnih pilića Hubbard genotipa, koji su hranjeni na 2 načina (standardnim/ekonomičnim) krmnim smešama koje su prisutne na našem tržištu. Seckana slama je korišćena kao prostirka. DTJ se karakteriše lezijama na metatarzalnim i digitalnim ventralnim delovima kože tabana živine. To je kontaktni dermatitis koji kod težih slučajeva utiče na prirast, zdravlje i dobrobit. Na kraju tova pilića, svi pilići su pregledani na prisustvo lezija na koži tabana. U ovom radu, ispitano je 39 pilića sa intenzitetom lezija na tabanskim jastučićima. Intenzitet lezija je ocenjen sa 2 (blage) i sa 3 (izražene) lezije. Mikološkim ispitivanjem utvrđeno je prisustvo gljivice/plesni Aspergillus fumigatus, kod 9 autoklaviranih promenjenih pilećih nogica. Histološkim ispitivanjem utvrdili smo postojanje hiperkeratoze, panikulitisa, zapaljenje dermisa i balonirajuću degeneraciju. Nismo utvrdili prisustvo fungalnih elemenata u koži tabana, nakon bojenja Gomory methenamin silver bojenjem. Ptice su posebno osetljive na infekciju A. fumigatus koji je klasifikovan kao alergijski uzročnik

Dermatitis tabanskih jastučića kod komercijalnih brojlera

In the present experiment, the presence of mycological agents in chickens with skin lesions on foot pads was investigated, in order to improve the health of broiler chickens. Foot-pad dermatitis (FPD) is described in the literature as dermatitis caused by multifactor causes. The presence of irritating chemical substances in the litter, genetic predispositions of hybrids, immune suppressive diseases of chickens, as well as the composition of commercial broiler diets that influence the occurrence of wet litter, are proven factors that contribute to a more intensive incidence of foot-pad dermatitis. Foot-pad dermatitis is a condition characterized by lesions on metatarsal and digital ventral skin of poultry feet. It is type of contact dermatitis which in severe cases shows slower weight gain, poor health condition and welfare. Taking into consideration the literature data, chickens reared in closed facilities where the bacterial and mycological agents are present partly in the litter if not properly disinfected, but also in the food or the chicken plumage, we wanted to investigate their presence in the skin of foot pads. The study was focused on incidence of foot-pad dermatitis in 500 one day old broiler chickens of Hubbard genotype, in two feeding (standard/economical) programs for broilers available on our market. Chopped straw was used as litter. At the end of fattening, all chickens were examined for the presence of lesions on the skin of the feet. Total of 39 chickens were allocated to the intensity of the lesion on foot pads rated as 2 (mild) and 3 (severe). In the mycological examination of the 9 samples of autoclaved chicken legs with lesions on the feet, we have isolated and identified the presence of fungi /mould Aspergillus fumigatus. The histopathology investigation, during which the tissue section from mild and severe skin foot pads was collected and stained using Hematoxylin and eosin, revealed the hyperkeratosis, panniculitis, ballooning degeneration and dermis inflammation. No fungal elements were observed in the sections of pads stained with Gomori methenamine silver. Birds are especially susceptible to infection with Aspergillus fumigatus. That species of fungus is also classified as allergenic factor.U ogledu je ispitivano prisustvo mikoloških agenasa kod pilića sa lezijama na koži tabanskih jastučića, u cilju poboljšanja zdravlja brojlerskih pilica. Dermatitis na tabanskim jastučićima (DTJ) je u literaturi multifaktornog porekla. Postojanje iritantnih hemijskih supstanci u prostirci, genetske predispozicije hibrida, imunosupresivne bolesti pilića, kao i sastav komercijalnih smeša za ishranu brojlera koje utiču na pojavu vlažne prostirke, su dokazani faktori koji doprinose intenzivnijoj pojavi DTJ. Imajući u vidu ove podatke iz literature, pilići koji se gaje u zatvorenom prostoru u kome su prisutni bakterijski i mikološki agensi delom u prostirci ako nije na adekvatan način dezinfikovana, u hrani ili na perju pilića, želeli smo da ispitamo njihovo prisustvo u koži tabanskih jastučića. Ispitivanje je bazirano na pojavi DTJ u ogledu 500 jednodnevnih pilića Hubbard genotipa, koji su hranjeni na 2 načina (standardnim/ekonomičnim) krmnim smešama koje su prisutne na našem tržištu. Seckana slama je korišćena kao prostirka. DTJ se karakteriše lezijama na metatarzalnim i digitalnim ventralnim delovima kože tabana živine. To je kontaktni dermatitis koji kod težih slučajeva utiče na prirast, zdravlje i dobrobit. Na kraju tova pilića, svi pilići su pregledani na prisustvo lezija na koži tabana. U ovom radu, ispitano je 39 pilića sa intenzitetom lezija na tabanskim jastučićima. Intenzitet lezija je ocenjen sa 2 (blage) i sa 3 (izražene) lezije. Mikološkim ispitivanjem utvrđeno je prisustvo gljivice/plesni Aspergillus fumigatus, kod 9 autoklaviranih promenjenih pilećih nogica. Histološkim ispitivanjem utvrdili smo postojanje hiperkeratoze, panikulitisa, zapaljenje dermisa i balonirajuću degeneraciju. Nismo utvrdili prisustvo fungalnih elemenata u koži tabana, nakon bojenja Gomory methenamin silver bojenjem. Ptice su posebno osetljive na infekciju A. fumigatus koji je klasifikovan kao alergijski uzročnik

Razlike u masno kiselinskom sastavu mesa između gološijana i dva komercijalna hibrida pilića

Chicken meat from intensive broiler production have different quality compared with meat from native chicken breeds and chicken from a free range production system. The aim of the present study was to evaluate differences in fatty acids content of meat of Naked Neck chickens rared in free range system and two commercial broiler breeds (Cobb 308 and Hybro G+) reared in conventional production system. The trial involved 100 chickens per group. Fattening period lasted 42 days for conventional system and 84 days for free range system. Breast meat of Naked Neck chicken had statistically more 16:0 content compared with Cobb 308 (p≤0.05), where in thigh meat the highest content of 16:0 was determined in Hybro G+ breed. Differences for fatty acids composition of breast meat between breeds were also established for 14:0, 17:0, 18:0, 16:1, 18:1, 18:2, 20:2, 22:1 and 22:5 fatty acid. The Naked Neck group showed the highest (p≤0.05) percentage of SFA and the Cobb 308 showed the lowest in breast meat. Thigh SFA were also significantly different (p≤0.05) between breeds, where Naked Neck chickens had the lowest and Hybro G+ the highest values. Breast MUFA were higher in Hybro G+ than in Naked Neck or Cobb 308 chickens, and thigh MUFA content had opposite trend and it was the lowest in Hybro G+ chicken. In conclusion, free ranged Naked Neck chickens have been shown to have significant different fatty acid composition compared with broiler chickens reared in conventional system.Cilj istraživanja je bio da se utvrdi razlika u masnokiselinskom sastavu mesa gološijana gajenih u slobodnom sistemu i dva komercijalna brojlerska hibrida (Cobb 308 i Hybro G+ gajenih u konvencionalnom proizvodnom sistemu. U ogledu je bilo po 100 pilića po grupi. Tov u konvencionalnom sistemu je trajao 42 dana, a u slobodnom sistemu 84 dana. Meso grudi gološijana sadržalo je statistički više 16:0 u odnosu na Cobb 308 (p≤0.05), dok je meso karabataka hibrida Hybro G+ imalo veći sadržaj 16:0 masnih kiselina. Sadržaj masnih kiselina u mesu grudi između hibrida je bio različit za 14:0, 17:0, 18:0, 16:1, 18:1, 18:2, 20:2, 22:1 i 22:5 masne kiseline. Zaključno, može se reći da je u mesu gološijana gajenih u slobodnom sistemu utvrđen statistički značajno različit sadržaj masnih kiselina u poređenju sa brojlerima gajenih u konvencionalnom sistemu

Comparative determination of lead concentrations in the air of the working environment by x-ray fluorescence spectroscopy and the spectrophotometric method

Za određivanje koncentracije olova u vazduhu u autoservisnoj radionici -Autovojvodina- u Novom Sadu primijenjene su metode rendgenske fluorescentne spektroskopije i spektrofotometrije. Rendgenski fluorescentni spektrometar baždaren je apliciranjem poznatih koncentracija Pb(N03)i na filter-papir. Vazduh je uzorkovan filtracijom poznatog volumena kroz filter-papir. Nađena koncentracija olova u vazduhu bila je ispod maksimalne dozvoljene koncentracije. U radu su izneseni i rezultati dobiveni spektrofotometrijskom metodom te su nađene razlike od 10 do 30% u odnosu na rezultate dobivene rendgenskom fluorescentnom spektroskopijom.The x-ray fluorescence spectroscopy and the spectrophotometric method were used to determine lead concentrations in the air of an auto repair shop. The calibration of the x-ray spectrometer was performed with known concentrations of water solutions of Pb(N03), on filter paper. Samples were prepared by filtration of measured volumes of air. The lead amounts determined were below the maximum permissible concentrations. The results obtained with the spectrophotometric method are also given showing discrepancies between 10 and 30 per cent if compared with the results of x-ray fluorescence spectroscopy

Recent initiatives in the Republic of Srpska to enhance appropriate use of antibiotics in ambulatory care : their influence and implications

Introduction:There are increasing concerns world-wide with growing rates of antibiotic resistance necessitating urgent action. There have been a number of initiatives in the Republic of Srpska in recent years to address this and improve rational antibiotic prescribing and dispensing despite limited resources to fund multiple initiatives. Objective: Analyse antibiotic utilization patterns in the Republic of Srpska following these multiple initiatives as a basis for developing future programmes in the Republic if needed. Methods: Observational retrospective study of total outpatient antibiotic utilization from 2010 to 2015, based on data obtained from the Public Health Institute, alongside documentation of ongoing initiatives to influence utilization. The quality of antibiotic utilisation principally assessed according to ESAC, ECDC and WHO quality indicators and DU 90% (the drug utilization 90%) profile as well as versus neighbouring countries. Results: Following multiple initiatives, antibiotic utilization remained relatively stable in the Republic at 15.6 DIDs to 18.4 DIDs, with a decreasing trend in recent years, with rates comparable or lower than neighbouring countries. Amoxicillin and the penicillins accounted for 29%-40% and 50% of total utilization, respectively. Overall, limited utilization of co-amoxiclav (7% - 11%), cephalosporins, macrolides and quinolones, as well as low use of third and fourth generation cephalosporins versus first and second cephalosporins. However, increasing utilization of co-amoxiclav and azithromycin, as well as higher rates of quinolone utilization compared to some countries, was seen. Conclusions: Multiple interventions in the Republic of Srpska in recent years have resulted in one of the lowest utilisation of antibiotics when compared with similar countries, acting as an exemplar to others. However, there are some concerns with current utilisation of co-amoxiclav and azithromycin which are being addressed. This will be the subject of future research activities

Recent initiatives in the Republic of Srpska to enhance appropriate use of antibiotics in ambulatory care : their influence and implications

Introduction:There are increasing concerns world-wide with growing rates of antibiotic resistance necessitating urgent action. There have been a number of initiatives in the Republic of Srpska in recent years to address this and improve rational antibiotic prescribing and dispensing despite limited resources to fund multiple initiatives. Objective: Analyse antibiotic utilization patterns in the Republic of Srpska following these multiple initiatives as a basis for developing future programmes in the Republic if needed. Methods: Observational retrospective study of total outpatient antibiotic utilization from 2010 to 2015, based on data obtained from the Public Health Institute, alongside documentation of ongoing initiatives to influence utilization. The quality of antibiotic utilisation principally assessed according to ESAC, ECDC and WHO quality indicators and DU 90% (the drug utilization 90%) profile as well as versus neighbouring countries. Results: Following multiple initiatives, antibiotic utilization remained relatively stable in the Republic at 15.6 DIDs to 18.4 DIDs, with a decreasing trend in recent years, with rates comparable or lower than neighbouring countries. Amoxicillin and the penicillins accounted for 29%-40% and 50% of total utilization, respectively. Overall, limited utilization of co-amoxiclav (7% - 11%), cephalosporins, macrolides and quinolones, as well as low use of third and fourth generation cephalosporins versus first and second cephalosporins. However, increasing utilization of co-amoxiclav and azithromycin, as well as higher rates of quinolone utilization compared to some countries, was seen. Conclusions: Multiple interventions in the Republic of Srpska in recent years have resulted in one of the lowest utilisation of antibiotics when compared with similar countries, acting as an exemplar to others. However, there are some concerns with current utilisation of co-amoxiclav and azithromycin which are being addressed. This will be the subject of future research activities

Comparison of multianalyte proficiency test results by sum of ranking differences, principal component analysis, and hierarchical cluster analysis

Sum of ranking differences (SRD) was applied for comparing multianalyte results obtained by several analytical methods used in one or in different laboratories, i.e., for ranking the overall performances of the methods (or laboratories) in simultaneous determination of the same set of analytes. The data sets for testing of the SRD applicability contained the results reported during one of the proficiency tests (PTs) organized by EU Reference Laboratory for Polycyclic Aromatic Hydrocarbons (EU-RL-PAH). In this way, the SRD was also tested as a discriminant method alternative to existing average performance scores used to compare mutlianalyte PT results. SRD should be used along with the z scores-the most commonly used PT performance statistics. SRD was further developed to handle the same rankings (ties) among laboratories. Two benchmark concentration series were selected as reference: (a) the assigned PAH concentrations (determined precisely beforehand by the EU-RL-PAH) and (b) the averages of all individual PAH concentrations determined by each laboratory. Ranking relative to the assigned values and also to the average (or median) values pointed to the laboratories with the most extreme results, as well as revealed groups of laboratories with similar overall performances. SRD reveals differences between methods or laboratories even if classical test(s) cannot. The ranking was validated using comparison of ranks by random numbers (a randomization test) and using seven folds cross-validation, which highlighted the similarities among the (methods used in) laboratories. Principal component analysis and hierarchical cluster analysis justified the findings based on SRD ranking/grouping. If the PAH-concentrations are row-scaled, (i.e., z scores are analyzed as input for ranking) SRD can still be used for checking the normality of errors. Moreover, cross-validation of SRD on z scores groups the laboratories similarly. The SRD technique is general in nature, i.e., it can be applied to any experimental problem in which multianalyte results obtained either by several analytical procedures, analysts, instruments, or laboratories need to be compared. [Figure not available: see fulltext.] © 2013 Springer-Verlag Berlin Heidelberg

PAHs, PCBs, PBDEs and Pesticides in Cold-Pressed Vegetable Oils

The aim of this study was to investigate levels of polychlorinated biphenyls (marker and dioxin-like congeners), polycyclic aromatic hydrocarbons (EPA 15 + 1), polybrominated diphenyl ethers (14 predominant congeners) and pesticides (74 compounds) in various cold-pressed vegetable oils. Poppy seed oil, rapeseed oil, sesame seed oil, pumpkinseed oil, hempseed oil, linaire oil, borage oil and evening star oil were investigated. Results of this study revealed that concentrations of PCBs, PBDEs and PAHs were low in majority of the investigated samples. However, high concentrations of organophosphorus insecticides were found. Chlorpyrifos methyl and pirimiphos methyl were the pesticide residues most commonly found in the studied oils. Concentration of 15 + 1 EPA PAHs was within the 17.85–37.16 μg kg−1 range, concentration of (marker) PCBs varied from 127 to 24,882 pg g−1, dioxin-like TEQ values were below 0.1 pg TEQ g−1. Concentration of PBDEs was below LOQ in most cases