41 research outputs found
Cytocompatibility of Novel Algae-PLA Membranes For Guided Bone Regeneration At The Level of Types I, III And V Collagen Expression
Statement of the problem: In recent years, the development of innovative
and increasingly optimized barrier membranes has focused on marine algae,
which as a biopolymer can form a membrane composite together with
polylactic acid, thus a combination could show numerous advantages such as
antioxidant, antitumor, antibacterial, antiviral as well as antiallergic properties.
Furthermore, algae can be produced in an ecologically sustainable way and offer
an alternative for patients who refuse treatment with bovine or porcine derived
membranes due to ethical or religious reasons.Objective: In this study, four different algal membranes were evaluated
for their cytocompatibility with cultured human fibroblasts and osteoblasts.Materials & Methods: Mem-Lok® (Collagen Matrix, New Jersey, USA)
as a resorbable collagen membrane and ArgonautTM (Botiss Biomaterials, Zossen,
Germany) as a native pericardium GBR/GTR membrane served as reference
membranes (RMs). As the negative control cells incubated with normal
culture medium only were used. In addition to the cell viability and proliferation
assays water soluble tetrazolium (WST), MTT and BrdU, a real time semiquantitative
real time PCR (RT-PCR) was developed to investigate in vitro cytocompatibility
at the level of types I, III and V collagen expression. A sandblasted-
large grid-acid ached titanium surface (Dentcon® Dental Implant Systems,
Ankara, Turkey) served as a positive inactive control group for osteoblastic
cytocompatibility.Results: For human osteoblasts, the algal membranes showed very good
proliferation levels in WST-1, MTT as well as BrdU, indicating cytocompatibility.
Examination of the expression behavior of type I, type III, and type V collagen
genes showed no evaluable results. However, the RT-PCR should be repeated
with the incorporated optimizations to be able to make a statement regarding
the success of bone, skin, and connective tissue regeneration after a
possible application of the membrane in maxillofacial injury treatment.Conclusion: The investigated collagen types are essential for a proper
healing of defects in both soft and bone tissue, as they have fundamental functions
such as stability and structural integrity of the tissues
Investigation of antibacterial activities of ethanol and methanol extracts of some marine algae species on yersinia ruckeri
Bu çalışmada Liagora ceranoides (Rhodophyta), Halopteris scoparia (Ocrophyta), Padina pavonica (Ocrophyta) ve
Sargassum vulgare (Ocrophyta) alglerinin etanol ve metanol ekstraktlarının hasta gökkuşağı alabalığı (Oncorhynhus
mykiss)’ndan izole edilen Yersinia ruckeri suşları üzerine antibakteriyel aktiviteleri değerlendirilmiştir. Ayrıca, suşların
çeşitli antibiyotiklere duyarlılıkları da çalışılmıştır. Balıklardan izole edilen suşlar fenotipik testler, API 20E hızlı
tanı kiti ve PZR sonuçlarına göre Yersinia ruckeri olarak tanımlanmıştır. Çalışmada L. ceranoides, H. scoparia, P.
pavonica ve S. vulgare makroalglerinin metanol ekstraktlarının Y. ruckeri’ye karşı antibakteriyel etki göstermediği
bulunmuştur. P. pavonica’nın etanol ekstraktı Y. ruckeri’ye karşı düşük antibakteriyel aktivite göstermiştir. L.
ceranoides, H. scoparia ve S. vulgare makroalglerinin etanol ekstraktlarının bakteriye karşı antibakteriyel aktivite
göstermediği tespit edilmiştir. Antibiyogram test sonuçlarına göre suşların ampisilin, flumekuin, oksitetrasiklin ve
trimetoprime duyarlı oldukları bulunmuştur. Suşlar eritromisine karşı orta derecede direnç göstermiştir. Sonuç
olarak, alglerin antibakteriyel aktiviteye sahip olduklarının bildirilmesine karşın her alg türünün antibakteriyel
aktivite gösteremeyeceği anlaşılmıştır.In this study, antibacterial activities of extracts of Liagora ceranoides (Rhodophyta), Halopteris scoparia (Ocrophyta),
Padina pavonica (Ocrophyta) and Sargassum vulgare (Ocrophyta) on Yersinia ruckeri strains isolated from sick rainbow
trout (Oncorhynhus mykiss) were evaluated. Also, susceptibilities of the strains against various antibiotics were
studied. The strains isolated from the sick fish were identified as Yersinia ruckeri according to the results of the
phenotypic tests, the API 20E rapid diagnostic kit and PCR. In the study, it was found that the methanol extracts
of L. ceranoides, H. scoparia, P. pavonica and S. vulgare did not show antibacterial activity against Y. ruckeri. The
ethanol extract of P. pavonica showed low antibacterial activity against Y. ruckeri. The ethanol extracts of L.
ceranoides, H. scoparia and S. vulgare macroalgae did not show antibacterial activity against the bacterium. According
to the results of the antibiogram test, it was found that the strains were sensitive against ampicillin, flumequine,
oxytetracycline and trimethoprime. The strains showed intermediate resistance against erythromycine. In
conclusion, although it was reported that algae had antibacterial activities, it was understood that every algae
species could not exhibit antibacterial activity
Unpublished Mediterranean and Black Sea records of marine alien, cryptogenic, and neonative species
To enrich spatio-temporal information on the distribution of alien, cryptogenic, and
neonative species in the Mediterranean and the Black Sea, a collective effort by 173
marine scientists was made to provide unpublished records and make them open
access to the scientific community. Through this effort, we collected and harmonized
a dataset of 12,649 records. It includes 247 taxa, of which 217 are Animalia, 25 Plantae
and 5 Chromista, from 23 countries surrounding the Mediterranean and the Black
Sea. Chordata was the most abundant taxonomic group, followed by Arthropoda,
Mollusca, and Annelida. In terms of species records, Siganus luridus, Siganus rivulatus,
Saurida lessepsianus, Pterois miles, Upeneus moluccensis, Charybdis (Archias)
longicollis, and Caulerpa cylindracea were the most numerous. The temporal
distribution of the records ranges from 1973 to 2022, with 44% of the records in
2020–2021. Lethrinus borbonicus is reported for the first time in the Mediterranean
Sea, while Pomatoschistus quagga, Caulerpa cylindracea, Grateloupia turuturu,
and Misophria pallida are first records for the Black Sea; Kapraunia schneideri is
recorded for the second time in the Mediterranean and for the first time in Israel;
Prionospio depauperata and Pseudonereis anomala are reported for the first time
from the Sea of Marmara. Many first country records are also included, namely:
Amathia verticillata (Montenegro), Ampithoe valida (Italy), Antithamnion
amphigeneum (Greece), Clavelina oblonga (Tunisia and Slovenia), Dendostrea cf.
folium (Syria), Epinephelus fasciatus (Tunisia), Ganonema farinosum (Montenegro),
Macrorhynchia philippina (Tunisia), Marenzelleria neglecta (Romania), Paratapes
textilis (Tunisia), and Botrylloides diegensis (Tunisia).peer-reviewe
Saros Körfezi (Ege Denizi, Türkiye) kıyılarının sahil ve derin deniz ALG florası
Bu araştırmada, Saros Körfezi kıyılarında yayılış gösteren deniz algleri çalışılmıştır. Saros Körfeziʼnde 64 Heterokontophyta, 68 Chlorophyta ve 124 Rhodophyta olmak üzere toplam 256 tane takson tespit edilmiştir