Kemal Kurdaş ve ODTÜ

Ankara : İhsan Doğramacı Bilkent Üniversitesi İktisadi, İdari ve Sosyal Bilimler Fakültesi, Tarih Bölümü, 2014.This work is a student project of the The Department of History, Faculty of Economics, Administrative and Social Sciences, İhsan Doğramacı Bilkent University.by Ünsal, Mehmet Süha

Oxidative chemical vapor deposition (oCVD) synthesis of molecularly imprinted polypyrrole nanotube for the detection of CA-125 protein

Field-effect transistor (FET) based biosensors have been widely used in various applications such as medical diagnosis, health and environmental monitoring. The performance of the FET biosensors are determined by anchoring specific probes on the conducting channel for target biomolecules. In the past two decades, molecularly imprinted polymers (MIPs) have attracted much attention as a robust and cost-effective alternative to natural bioreceptors such as enzymes and antibodies. MIPs are artificial template-made receptors which have the ability to recognise specific target molecules. Here, we present a novel FET biosensor incorporated with a molecularly imprinted polypyrrole nanotube (MIPN) for the selective detection of CA-125 ovarian cancer biomarkers. Polypyrrole (PPy) nanotube used in the FET biosensor has been obtained via oxidative chemical vapor deposition (oCVD) which is a highly efficient solvent-free, vacuum-based technique for the synthesis of conductive polymers. Thanks to the flexibility of this method, MIPN has been conveniently synthesized by coating conformally the sacrificial porous structured templates in the presence of target protein. Particularly, imprinting the target molecule on the nanotube surface has provided extremely high surface area which leads to enhancement in selectivity of the sensor. Furthermore, the oCVD synthesis has enabled higher electrical conductivity for the resulting PPy nanotube by tuning deposition parameters such as oxidant/monomer ratio, substrate temperature and reaction pressure. Synthesized MIPN has been integrated onto the interdigitated array Au electrode to make a conductive bridge between source and drain terminals and biosensor platform has been assembled from chip device, microfluidic channel and custom made chip holder for real-time measurement. The present study has provided useful insight into preparing a novel molecularly imprinted polymer nanotube with various target protein molecules by using oCVD technique

Development of molecularly imprinted polymer-based FET biosensor via oxidative chemical vapor deposition

Biosensors have been attracting an increasing interest in various fields including medical diagnosis and health monitoring due to its rapid, selective and sensitive detection of target analyte in real-time. As biological receptors require special environmental conditions, they limit the durability and long-term storage of the biosensors. However, molecularly imprinted polymers (MIPs) are artificially synthesized to mimic the recognition of biological macromolecules at a significantly lower cost and no need for any special storage. Here, we reported the usability of the polypyrrole (PPy) MIP as a synthetic biorecognition element on a FET based biosensor to detect CA-125 ovarian cancer biomarker. We used oxidative chemical vapor deposition (oCVD) technique which allows the conformal and controlled thin film conductive polymers, to produce MIP onto a sacrificial layer. Synthesized MIP then integrated onto the interdigitated electrode arrays to make a conductive bridge between source and drain terminals for the development of the FET biosensing platform. The oCVD technique has a great potential to be used in the development of MIP-based biosensors for the detection of target proteins without wasting as in the traditional bulk polymerization method

Trends in the choice of antiseizure medications in juvenile myoclonic epilepsy: A retrospective multi-center study from Turkey between 2010 and 2020

Purpose:Valproic acid (VPA) is frequently used and effective in juvenile myoclonic epilepsy (JME). Recently, levetiracetam (LEV) has been suggested as a monotherapy in JME. This study aimed to evaluate antiseizure medication (ASM) use in patients with JME. Methods: Treatment choices in a total of 257 patients (age range 8–18 years, 152 girls, 105 boys) with JME diagnosed and treated between 2010 and 2020 were evaluated retrospectively. Seizure remission was defined as complete seizure control for at least 12 months. Results: Across the study period and entire patient group, VPA was most commonly chosen as the initial ASM (50.9%), followed by LEV (44.4%), and lamotrigine (4.7%). VPA was also the most frequent first choice in the subgroup of boys (73.3%), while LEV was the commonest first choice in girls (57.9%). The sex difference regarding the ASM of the first choice was statistically significant (p<0.001). While VPA was the most frequent initial ASM in the first 5 years of the study period (2010–2015,n = 66, 64%), LEV had taken over as the most popular first ASM in the last 5 years (n = 83, 53.9%, p = 0.005). The most frequent reasons for discontinuation were inefficacy for LEV and adverse effects for VPA (p = 0.001). During follow-up, 237 patients (92.2%) were seizure-free for at least 12 months, and 159 (61.9%) were also in electrographic remission. Seizure remission occurred earlier than electroencephalographic remission (p<0.001). Conclusion: This study revealed that LEV has become the most frequently chosen initial ASM in the treatment of JME. Although LEV appears to have a better adverse effect profile, VPA seems more likely to be effective in achieving seizure control

Epidemiological, Clinical, and Laboratory Features of Children With COVID-19 in Turkey

Objectives: The aim of this study is to identify the epidemiological, clinical, and laboratory features of coronavirus disease 2019 (COVID-19) in children