Aksaray İlinde abortus ve infertilite problemi olan süt ineklerinde neospora caninum insidansı

The contribution of animal husbandry to the national economy cannot be ignored. The causes of infertility are various and complex. Economic losses are increasing in dairy cattle breeding due to infectious and non-infectious causes. The percentage of abortion due to infectious agents is not fully known, but infectious agents lie in about 90% of the cases whose etiology can be determined. Neospora caninum is considered to be one of the most important abortion factors of cattle. Although, this protozoa is always ignored. It causes abortion in cows. In this study, it was aimed to detect N. caninum from the blood serum obtained from 137 dairy cattle brought to Aksaray University Faculty of Veterinary Medicine between 2017-2019 and suffering from abortion and infertility problems. For serological diagnosis, ELISA (Enzyme-Linked ImmunoSorbent Assay) test was performed. As a result, N. caninum agent was detected in 37.22 % (51/137) of the samples that belong to cattle with abortion and infertility problems. It was concluded that economic loss due to infertility in dairy cattle and protozoa, which are one of the infection factors, should be given importance.Hayvancılığın ülke ekonomisine katkısı yadsınamaz. İnfertilitenin nedenleri çeşitli ve karmaşıktır. Süt sığırcılığında bulaşıcı ve bulaşıcı olmayan nedenlerle ekonomik kayıplar artmaktadır. Enfeksiyöz ajanlara bağlı abortların yüzdesi tam olarak bilinmemekle birlikte, etiyolojisi belirlenebilen olguların yaklaşık %90'ında enfeksiyöz ajanlar bulunmaktadır. Neospora caninum sığırların en önemli abortus faktörlerinden biri olarak kabul edilmektedir. Bununla birlikte, bu protozoa her zaman göz ardı edilir. İneklerde abortusa, köpeklerde ise ölümcül sinir-kas hastalıklarına neden olur. Bu çalışmada, Aksaray Üniversitesi Veteriner Fakültesi'ne 2017-2019 yılları arasında getirilen abort ve infertilite sorunu yaşayan 137 süt sığırından elde edilen kan serumundan N. caninum'un saptanması amaçlanmıştır. Serolojik tanı için ELISA (Enzyme-Linked ImmunoSorbent Assay) testi yapıldı. Sonuç olarak abortus ve infertilite sorunu olan sığırlara ait örneklerin %37,22'sinde (51/137) N. caninum etkeni tespit edilmiştir. Süt sığırlarında infertiliteye bağlı ekonomik kayıplara ve enfeksiyon faktörlerinden biri olan protozoalara önem verilmesi gerektiği sonucuna varıldı

EFFECT OF LOCAL RIFAMYCIN APPLICATION ON EXPRESSION OF BMP-2 AND BONE REGENERATION

Objectives: The aim of this study was to evaluate effect of local rifamycin application on BMP-2 expression and bone healing.Materials and Methods: A standardized 5.0-mm- diameter critical size bone defect was created mandible angulus region. In the control group (8 rats) defects were left empty. In the Group 1 (n=8 rats) defect was irrigated with rifamycin solution and 25 mg rifamycin solution injected defect area at 1, 3, 7 days after surgery. In the group 2 (n=8 rats) defects were grafted with a gelatin sponge mixed 25 mg rifamycin solution. Rats were sacrificed at 21 days after surgery. Histological slides were prepared from defect site for both immunohistochemical analysis (bone morphogenetic protein-2 (BMP-2) antibody) and histomorphometric analysis. Data were analyzed using Mann Whitney U and Kruskall Wallis test.Results: The average new bone formation, number of osteoblast and new vessel formation count were increased more in both of experimental groups in comparison with control group. Anti-BMP-2 labelling (Cell count) was increased more in both of experimental groups in comparison with control group. Conclusion: Local rifamycin application has positive effects on BMP-2 expression and bone regeneration at critical sized bone defects

EFFECT OF LOCAL RIFAMYCIN APPLICATION ON EXPRESSION OF BMP-2AND BONE REGENERATION

Amaç: Bu çalışmanın amacı lokal rifamisin uygulamasının kemik iyileşmesi sırasında BMP-2 salınımı üzerine etkisinin değerlendirilmesidir. Materyal ve method: Rat mandibula angulus bölgesinde standart olarak 5 mm çapında kritik boyutta kemik defektleri oluşturulmuştur. Kontrol grubunda (8 rat) defektlere herhangi bir uygulama yapılmamıştır. Birinci deney grubunda (8 rat) defekt bölgesi rifamisin solüsyonu ile irrige edildikten sonra, defekt bölgesine 1, 3 ve 7. günlerde 25 mg rifamisin solüsyonu enjekte edilmiştir. İkinci deney grubunda (8 rat) defekt bölgesi 25 mg rifamisin solüsyonu ile karıştırılmış gelatin sponge ile greftlenmiştir. Cerrahiden 21 gün sonra ratlar sakrifiye edilmiştir. Defekt bölgesinden hem immünhistokimyasal analiz (kemik morfogenetik protein -2 antibody) için hem de histomorfometrik analiz için histolojik kesitler hazırlanmıştır. Elde edilen verilerin analizi Mann Whitney U ve Kruskall Wallis testi kullanılarak yapılmıştır. Bulgular: Deney grubunda kontrol grubuna göre ortalama yeni kemik formasyonu, osteoblast sayısı ve yeni damar oluşum sayısında artış olduğu görülmüştür. Her iki deney grubunda da anti-bmp-2 ile işaretlenmenin (hücre sayma) kontrol grubuna göre daha fazla olduğu görülmüştür. Sonuç: Kritik boyutta kemik defektlerine lokal olarak rifamisin uygulamasının BMP-2 salınımı üzerine pozitif etkileri olduğu tespit edilmiştir.Objectives: The aim of this study was to evaluate effect of local rifamycin application on BMP-2 expression and bone healing. Materials and Methods: A standardized 5.0-mm- diameter critical size bone defect was created mandible angulus region. In the control group (8 rats) defects were left empty. In the Group 1 (n8 rats) defect was irrigated with rifamycin solution and 25 mg rifamycin solution injected defect area at 1, 3, 7 days after surgery. In the group 2 (n8 rats) defects were grafted with a gelatin sponge mixed 25 mg rifamycin solution. Rats were sacrificed at 21 days after surgery. Histological slides were prepared from defect site for both immunohistochemical analysis (bone morphogenetic protein-2 (BMP-2) antibody) and histomorphometric analysis. Data were analyzed using Mann Whitney U and Kruskall Wallis test. Results: The average new bone formation, number of osteoblast and new vessel formation count were increased more in both of experimental groups in comparison with control group. AntiBMP-2 labelling (Cell count) was increased more in both of experimental groups in comparison with control group. Conclusion: Local rifamycin application has positive effects on BMP-2 expression and bone regeneration at critical sized bone defects

Oxidant-antioxidant status in bovine ephemeral fever

WOS: 000466333800024Bovine ephemeral fever is an economically important viral disease of cattle and water buffalo. In this study, it was aimed to investigate oxidant-antioxidant status in cattle with ephemeral fever disease. A total of 32 Holsteins aged between 12- 18 months kept under same care and feeding conditions were used. Activities of GPx, CAT and SOD and the levels of TAC, TOS, CP, SH and MDA were measured. Levels of CP and TAC were significantly (p<0,05) higher in IG compared to HG. Levels of SH and CP were significantly (p<0,05) lower and CAT activity were significantly (p<0,05) higher in RG compared with IG. Levels of SH and activity of GPx and were significantly (p<0,05) lower and CAT levels were significantly (p<0,05) higher in RG compared with HG. The results or this experiment suggest a possible relationship between oxidant/antioxidant balance in favor of antioxidants in ephemeral fever in cattle

Toilet training age and influencing factors: a multicenter study

Tarhan H, çakmak ö, Akarken İ, Ekin RG, ün S, Uzelli D, Helvacı M, Aksu N, Yavaşçan ö, Mutlubaş-özsan F, Cun S, Koç F, özkarakaş ö, İlbey Yö, Zorlu F. Toilet training age and influencing factors: a multicenter study. Turk J Pediatr 2015; 57: 172-176.To determine toilet training age and the factors influencing this in our country, 1500 children who had completed toilet training were evaluated in a multicenter study. the mean age of toilet training was 22.32±6.57 months. the duration it took to complete toilet training was 6.60±2.20 months on the average. in univariant analysis, toilet training age increased as the parental education level, specifically that of the mother, increased. the training age of children whose mothers had over 12 years of education differed significantly from that of children of mothers with less education. There was no significant difference in toilet training age with regard to the education level of the father, or the employment status of the mother. We also found significant differences with respect to family income level, toilet type and training method. in multivariant analysis, family income >5000 TL and use of a potty chair were determined to be factors affecting toilet training age. in conclusion, toilet training age in Turkey, a developing country, was found to be lower than that in developed countries