Experiences of Healthcare Professionals Providing Women’s Health Services to Asylum Seeking Women at the Hospitals

Objective: This study aimed to describe the experiences of healthcare professionals providing women’s health services to asylum seeking women at hospitals. Methods: A qualitative design was used in this study. The data were collected from thirty-four healthcare professionals providing women’s healthcare services to asylum seeking women through semi-structured, face-to-face, individual in-depth interviews. The data were evaluated using Colaizzi’s seven-step analysis method in the NVivo12 package program. Results: Three themes were identified (1) challenges, (2) reflections, and (3) needs. Healthcare professionals evaluated the process of providing women’s healthcare services to asylum seeking women from a positive and negative point of view. They stated that they should be supported personally, professionally, and as a health team in improving this experience. Conclusion: This study narrows the gap in the literature and expands the scope of existing knowledge concerning the healthcare professionals experience about asylum seeking women health care

Development of a sensitive primer extension method for direct detection and quantification of miRNAs from plants

PubMed: 32163493MicroRNAs (miRNAs) are short non-coding RNA molecules that regulate target gene expression in various organisms. Functional studies are therefore required to determine their temporal and spatial expression patterns. Primer extension has been used as a sensitive and reliable approach to identify miRNAs (?21-22 nt) in the mammalian system and can be used in other systems such as plants. However, a well-defined method is required for ease of application and reproducibility. Here, a radioactive primer extension method was developed for the quantitative detection of miRNAs found in total RNA samples from plants. As a proof of concept, miR173 and miR828 were detected by primer extension in total RNA samples isolated from Arabidopsis. The assay involved the extension reaction of the miRNA guide strand with a radiolabeled specific primer. Using a manual DNA sequencer, primers extended with reverse transcriptase were separated on a denaturing polyacrylamide gel. The gel was then dried and exposed to a PhosphorImager screen for size-dependent product identification up to a single base difference. Quantification was done based on the intensity of radioactive signals by normalizing the cDNA products to an internal control. The primer extension was proven to be efficient to detect and quantify miRNAs in plant total RNA samples without subsequent enrichment of low-molecular-weight RNA species. This method, optimized for Arabidopsis, can be applied to a wide variety of organisms for the detection and quantification of miRNAs as well as siRNAs. © 2020 Ulku Baykal. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Synthesis and antisense activities of DNA oligomers with hydrolytically active sites

Ph.D. - Doctoral Progra

Organizational Citizenship Behavior among Nurses: The Influence of Organizational Trust and Job Satisfaction

AIM: This study was conducted to determine the relationship between nurses' organizational citizenship behavior organizational citizenship behavior and organizational trust and job satisfaction

Relationships between the participation of a private hospital's employees in quality studies and their attitudes towards work and institution

Purpose: To define organizational culture in hospitals that have received quality certificates and to identify attitudes of healthcare personnel toward change

Personality Traits of Nurse Managers and Evaluation of Their Traits by Their Subordinates

AIM: To investigate personality traits (PT) of nurse managers (NM) and their subordinates' perceptions of these traits