Comparaison de différentes méthodes d'extraction d'acides dicaféoylquiniques à partir d'une plante halophile

AbstractThe aim of this study is the evaluation of different extraction methods for dicaffeoylquinic acids (diCQA), previously identified by a LC-DAD-ESI-QTOF dereplication strategy from a halophyte plant rich in polyphenols. Three different eco-friendly extraction methods are tested: microwave-assisted extraction (MAE), pressurized fluid extraction (PFE), supercritical fluid extraction (SFE). Various specific parameters are optimized for each of them. Global extraction yields are calculated for all extracts. Moreover, the extracts are analyzed by HPLC–DAD–ELSD, and the obtained profiles are compared to estimate qualitatively and semi-quantitatively their composition. The practicality of each technique is also discussed. The results show that the various parameters tested for PFE and MAE do not drastically affect the extraction of our interest compounds. However, the parameters tested on SFE are more decisive, such as the addition of a modifier in CO2, which allows the extraction of diCQA

Contribution of Supercritical Fluid Chromatography coupled to High Resolution Mass Spectrometry and UV detections for the analysis of a complex vegetable oil – Application for characterization of a Kniphofia uvaria extract

AbstractLipids are an important and diverse group of naturally occurring organic compounds present in all biological entities. They play many essential roles in the survival of the organisms. Analysis of lipids is extremely complex due to their large variety of chemistries. In this work, we describe an innovative method for the characterization of the lipid composition in a complex and unknown vegetable oil: an extract of Kniphofia uvaria seeds; using Supercritical Fluid Chromatography (SFC) coupled to Atmospheric Pressure Chemical Ionization (APCI), High-Resolution Mass Spectrometry (HRMS) and UV detections. Thanks to the properties of supercritical CO2 allowing the use of a large number of chromatographic columns, an effective separation for this complex matrix was obtained

Contribution of Supercritical Fluid Chromatography coupled to High Resolution Mass Spectrometry and UV detections for the analysis of a complex vegetable oil – Application for characterization of a Kniphofia uvaria extract

AbstractLipids are an important and diverse group of naturally occurring organic compounds present in all biological entities. They play many essential roles in the survival of the organisms. Analysis of lipids is extremely complex due to their large variety of chemistries. In this work, we describe an innovative method for the characterization of the lipid composition in a complex and unknown vegetable oil: an extract of Kniphofia uvaria seeds; using Supercritical Fluid Chromatography (SFC) coupled to Atmospheric Pressure Chemical Ionization (APCI), High-Resolution Mass Spectrometry (HRMS) and UV detections. Thanks to the properties of supercritical CO2 allowing the use of a large number of chromatographic columns, an effective separation for this complex matrix was obtained