Comparaison de différentes méthodes d'extraction d'acides dicaféoylquiniques à partir d'une plante halophile

Abstract

AbstractThe aim of this study is the evaluation of different extraction methods for dicaffeoylquinic acids (diCQA), previously identified by a LC-DAD-ESI-QTOF dereplication strategy from a halophyte plant rich in polyphenols. Three different eco-friendly extraction methods are tested: microwave-assisted extraction (MAE), pressurized fluid extraction (PFE), supercritical fluid extraction (SFE). Various specific parameters are optimized for each of them. Global extraction yields are calculated for all extracts. Moreover, the extracts are analyzed by HPLC–DAD–ELSD, and the obtained profiles are compared to estimate qualitatively and semi-quantitatively their composition. The practicality of each technique is also discussed. The results show that the various parameters tested for PFE and MAE do not drastically affect the extraction of our interest compounds. However, the parameters tested on SFE are more decisive, such as the addition of a modifier in CO2, which allows the extraction of diCQA