Sarkoplazmik Protein Elektroforezi ile Orthrias Insignis Euphraticus (Banarescu and Nalbant, 1964) ve Cyprinion Macrostomus (Heckel, 1843) Taksonomisinin İncelenmesi

In this study, SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) was applied to the sarcoplasmic proteins of Orthrias insignis euphyraticus (BALITORIDAE) and Cyprinion macrostomus (CYPRINIDAE) fish taken from Karakaya Dam Lake. The electrophoregram showed that there were differences between the two species in both the number of bands and the molecular weight (MW) of the sarcoplasmic proteins. While Cyprinion macrostomus had 18 protein bands, there were 20 protein bands in Orthrias insignis euphyraticus. On the other hand, the bands obtained from Orthrias insignis euphyraticus and numbered as 9th protein band with molecular weight of 29.5 kD and 12th band with MW of 27.2 kD were not found in Cyprinion macrostomus. 9th protein band with MW of 27.5 kD was only found in Cyprinion macrostomus. We concluded that comparisons of sarcoplasmic proteins might have practical importance in taxonomic classification.Bu çalışmada, SDS-PAGE (sodyum dodesil sülfat-poliakrilamid jel elektroforezi)’ne, Karakaya Baraj Gölü’nden yakalanan Orthrias insignis euphyraticus (BALITORIDAE) ve Cyprinion macrostomus (CYPRINIDAE) balıklarının sarkoplazmik proteinleri uygulandı. Elektroforegramda, sarkoplazmik proteinlerin band sayısı ve molekül ağırlıklarının iki tür arasında farklı olduğu görüldü. Cyprinion macrostomus 18 protein bandına sahipken Orthrias insignis euphyraticus’da 20 protein bandı vardı. Diğer taraftan, Orthrias insignis euphyraticus’dan elde edilen 29.5 kD moleküler ağırlığına sahip 9. protein bandı ve 27.2 kD moleküler ağırlığına sahip 12. protein bandı Cyprinion macrostomus’ta bulunmadı. 27.5 kD moleküler ağırlığına sahip 9. protein bandı yalnızca Cyprinion macrostomus’ta bulundu. Sarkoplazmik proteinlerin karşılaştırılmasının taksonomik sınıflandırmada pratik önemi olabileceğine karar verildi

A Taxonomical Study on Electrophoretic Pattern of Blood Serum Protein of Some Fish of Karakaya Dam Lake

In Ihis study, an electrophoretic comparison 01 serum blood proteins of two species 01 fish, Cspoeta trulla and Capoels capoeta umbls, were carried out. Study was carried between june and october in 1997. Using the Ran· dom Sampling Method, 92 C. capos's umbla and 86 C. trutta which the same age and sex were used. The iden· dification of species and subspecies were done though their metric and meristic characteristics. While 16 band pat· tern was observed in C. trutta, the number 01 protein bands for C. capoeta umbla was 11 . From the electrophorelic mobility 8 bands of species gave similar pattern, but the rest was seen to be different. As a result, we concluded that, there is a %50 similarity between C. trulta and C. capoeta umbla based on their serum blood proteins.Bu ara~IIfITla haziran ile ekim 1997 tarihleri arasmda gergekle~tirildi. Ar~t,"Tlada, y,*lan ve cinsiyetleri aynl alan 92 adet Capoeta caposts umbJa ve 86 adet Capoet8 tlUtta kullanllch. SOz konusu ballklann metrik ve meristik de· gerierine gOre klasik olarak tur ve all lOr tespili yaplldl. C. IlUtta ve C. capoela umbla'iann kan serum proteinlerinin SOS·Poliakriiamid Jel Elektroforez (SOS·PAGE) yOntemi ile incelenerek yapllan fUr ve alt tOr 9all~maslnda , C. tlUtt{ J'd8 16, C. capoeta umbla'da ise 11 protein bandl tespit edildi. C. trutta'nln eleklroforegrammda diger lOre gOre 8 farkl1 protein band! gOzlendi. Bunun yanl sira 8 aOOt protein bandl bu iki ballk arasmda benzerlik gOsterdi. SOS-PAGE lie elde edilen verilere gOre, C. tlUtta va C. capoeta umbla arasmda eleklroforegamda olu$an protein banllan baklmmdan %50'lik farkllllk tasp!1 edilmi~ ve bunun laksOrlOmik ayldan Onemfi ofdugu sonucuna vanlml~tlr

Tekstil Fabrikası Atığının Cyprinion macrostomus, Karaciğer, Böbrek ve Kan Dokusuna Olan Biyokimyasal, Histopatolojik ve Genotoksik Etkisi

Bu araştırmada; 3 yaşındaki Cyprinion macrostomus' lar 10 ve 35 gün boyunca o/o2,5' lik tekstil fabrikası atığı içinde yaşatıldılar. Sonuçta; 35 günlük grupta karaciğer ve böbrek glutatyon (GSH) konsantrasyonları kontrole göre anlamlı şekilde azalırken 35 günliik grubun karaciğer ve böbrek malondialdehit (MDA) konsantrasyonlan kontrole göre anlamlı şekilde artmıştı. 35 günlük grubun karaciğer dokusunda vakuolcr dejcnerasyon şeldinde nekrozların oluşması gibi histopatolojik değişimler gözlendi. Mikronukleus sayısında anlamlı bir artış aynı grupta da gözlendi....

The effect of hysteroscopic polypectomy on the gene expression of endometrial receptivity markers: HOXA10, HOXA11 and LIF

The aim of this study is to determine the effect of hysteroscopic polypectomy on the mRNA expression levels of the endometrial receptivity markers, namely, homeobox A10 (HOXA10), homeobox A11 (HOXA11) and leukemia inhibitory factor (LIF). Twenty-five reproductive-aged women with endometrial polyps underwent hysteroscopy. Samples were taken at the mid-secretory phase using hysteroscopic polypectomy and 4 months after polypectomy, and the change in mRNA expression levels of normalized HOXA10, HOXA11 and LIF genes were determined using Reverse Transcription Quantitative Real Time-Polymerase Chain Reaction (RT-qPCR). The results show that mRNA levels of HOXA10 and HOXA11 taken prior to surgery and 4 months after the complete hysteroscopic removal of polyps were not significantly different (P=0.79 and P=0.14, respectively). Moreover, a marked difference could not be obtained between preoperative and postoperative endometrial LIF mRNA expression levels (P=0.86). As a conclusion, these results indicate that mRNA levels of HOXA10, HOXA11 and LIF genes, three of molecular markers of endometrial receptivity, are not affected by hysteroscopic polypectomy

Effects of central infusion of irisin on the energy metabolism and glucose uptake in rats

3rd International Congress of the Turkish-Neuroendocrinology-Society -- JUN 29-JUL 01, 2018 -- Malatya, TURKEYWOS: 000445952400069…Turkish Neuroendocrinol SocietyTUBITAK [214S205]This study was supported by TUBITAK (Project no: 214S205)

Effects of peripheral irisin infusion on the glucose uptake in high-fat-diet-induced obese rats

WOS: 000453220100146…TUBITAK [214S205]This study was supported by TUBITAK (Project no: 214S205)

Cannabinoid type 2 receptor agonist JWH-133, attenuates Okadaic acid induced spatial memory impairment and neurodegeneration in rats

Aim: Cannabinoid system has various physiological roles such as neurogenesis, synaptic plasticity and emotional state regulation in the body. The presence of cannabinoid type 2 receptor (CB2), a member of the cannabinoid system, was detected in different regions of the brain. CB2 receptor plays a role in neuroinflammatory and neurodegenerative processes. We aimed to determine the possible effect of CB2 agonist JWH-133 in Okadaic acid (OKA)-induced neurodegeneration model mimicking Alzheimer's Disease (AD) through tau pathology. Materials and methods: In this study, 40 Sprague Dawley male rats were divided into 4 groups (Control, Sham, OKA, OKA + JWH-133). Bilateral intracerebroventricular (icv) injection of 200 ng OKA was performed in the OKA group. In the OKA + JWH-133 group, injection of JWH-133 (0.2 mg/kg) was performed intraperitoneally for 13 days different from the group of OKA. Morris water maze test was used to evaluate the spatial memory. Levels of caspase-3, phosphorylated tau (ser396), amyloid beta (A beta), tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) levels in brain cortex; and the hippocampus regions were examined by immunohistochemical methods. Key findings: In the OKA group, caspase-3, phosphorylated tau (ser396), A beta, IL-1 beta levels were higher in the cortex and hippocampus than in the other groups. The implementation of the JWH-133 reversed the increments in these parameters, and also prevented spatial memory impairment