Phosphorus-nitrogen compounds. Part 48. syntheses of the phosphazenium salts containing 2-pyridyl pendant arm: structural characterizations, thermal analysis, antimicrobial and cytotoxic activity studies

533-550The phosphazenium salts (protic ionic liquids, PILs/protic molten salts, PMOSs) (6a-6d and 7a) of the free phosphazene bases (4a-4d and 5a) have been prepared by the reactions of the corresponding cyclotriphosphazenes with the bulky gentisic acid. The structures of the PMOS have been evaluated using the elemental analyses, FTIR, 1H, 13C{1H} and 31P{1H} NMR data. The molecular and crystal structures of 4a and 6c are established by X-ray crystallography. The thermal properties of the PMOS are determined using TG and DTA techniques. On the other hand, the antimicrobial activities of the free phosphazene bases (4a-4d and 5a-5d) and PMOSs (6a-6d and 7a) are screened against the selected bacteria and yeast strains. The antimicrobial activities of the free phosphazene bases and the PMOSs are compared. The interactions of the phosphazenes and their salts with plasmid DNA are elucidated by the agarose gel electrophoresis. The evaluations of the cytotoxic activities of these compounds are also studied against to L929 fibroblast and breast cancer cells (MDA-MB-231)

Phosphorus-nitrogen compounds. Part 48. Syntheses of the phosphazenium salts containing 2-pyridyl pendant arm: Structural characterizations, thermal analysis, antimicrobial and cytotoxic activity studies

The phosphazenium salts (protic ionic liquids, PILs/protic molten salts, PMOSs) (6a-6d and 7a) of the free phosphazene bases (4a-4d and 5a) were prepared by the reactions of the corresponding cyclotriphosphazenes with the bulky gentisic acid. The structures of the PMOS were evaluated using the elemental analyses, FTIR, 1H, 13C{1H} and 31P{1H} NMR data. The molecular and crystal structures of 4a and 6c were established by X-ray crystallography. The thermal properties of the PMOS were determined using TG and DTA techniques. On the other hand, the antimicrobial activities of the free phosphazene bases (4a-4d and 5a-5d) and PMOSs (6a-6d and 7a) were screened against the selected bacteria and yeast strains. The antimicrobial activities of the free phosphazene bases and the PMOSs were compared. The interactions of the phosphazenes and their salts with plasmid DNA were elucidated by the agarose gel electrophoresis. The evaluations of the cytotoxic activities of these compounds were also studied against to L929 fibroblast and breast cancer cells (MDA-MB-231).  

Biological activities and DNA interactions of aqueous extract of Phlomis linearis (Boiss. & Bal.)

Phlomis linearis Boiss. & Bal. of the Lamiaceae family is one of the endemic species in Turkey, i.e., growing in the east, central, and southeast parts of Anatolia and used for herbal tea. This study was designed to identify the biochemical and bioactivity properties of this endemic species by DPPH scavenging activity, metal chelating activity, total phenolic content, HPLC-DAD analysis, and MTT assay. Furthermore, the plant extract was evaluated for its antimicrobial activity against bacteria and fungi by using the microdilution method. The interactions between extract and plasmid DNA and their restriction endonuclease reactions were investigated by agarose gel electrophoresis. To support our hypothesis, we performed a molecular docking analysis. The DPPH scavenging activity of the plant extract was 53.86 ± 0.50 µg/ml in terms of IC50 value. The IC50 value of the plant extract was determined as 14.71 ± 4.01 mg/ml for metal chelating assay. The phenolic content of the extract was 231.55 ± 2.11 mg/g dry weight expressed as gallic acid equivalents (GAE). HPLC-DAD results revealed that the phenolic compounds were mainly derivatives of rosmarinic acid, chlorogenic acid, luteolin, luteolin-7-glycoside, luteolin derivatives, rutin derivatives, and apigenin derivatives. Besides, the cytotoxic activity of the plant extract against L929 fibroblast, H1299 non-small-cell lung carcinoma, and Caco-2 colorectal adenocarcinoma cell lines was determined by MTT assay. Phenolic content and molecular docking results correlated with each other

The analysıs of ısolatıon and antıbıotıc resıstance of gram ( - ) bacterıa ısolated from some freshwater fıshes ınhabıted the rıvers ın ankara and ıts envıronment

Bu araştırmada Ankara (Türkiye) ili çevresindeki Kızılırmak ve Sakarya akarsularından alınan balık (deri, solungaç, bağırsak), su ve sediment örneklerinden Aeromonas ve Pseudomonas cinsi bakterilerin ve Enterobacteriaceae familyasından E. coli, K. pneumoniae, C. freundii, P. Vulgaris ve E. agglomerans'ın izolasyon, identifikasyon ve antibiyotik duyarlılık testleri yapılmıştır. İzole edilen toplam 116 gram negatif bakterinin %37,9'u Aeromonas spp., %17,2'si Pseudomonas spp., %15,5'i E. coli, %10,3'ü K.pneumoniae, %7,7'si C. freundii, %6,8'i P. vulgaris ve %4,3'ü E. Agglomerans olarak tanımlanmıştır. Disk difüzyon ve mikrodilüsyon yöntemiyle elde edilen antibiyotik direnç dağılımları sırasıyla; Aeromonas izolatlarında tikarsiline (%54,4) ve amikasine (22,8) - (%0), P. aeruginosa izolatlarında tetrasikline (%71,4) – (%57,1) ve tikarsiline (%42,9), diğer Pseudomonas izolatlarında tetrasikline (%69,3) – (%30,7), E. coli izolatlarında tikarsiline (%83,4) ve gentamisine (%50,1) – (%11,1), K. pneumoniae izolatlarında tikarsiline (%50), sefotaksime (%41,6) – (%16,7), siprofloksasine (%91,7) – (%16,7), amikasine (%25) – (%8,3), kloramfenikole (%83,4) - (%16,7), tetrasikline (%100) – (%100), seftazidime (%100) – (%0) ve imipeneme (%33,3) – (%0), C. Freundii izolatlarında tetrasikline (%44,5) – (%0) ve seftazidime (%66,7) – (%11,1), P. vulgaris izolatlarında tikarsiline (%25), amikasine (%50) – (%12,5), tetrasikline ise (%100) – (%100), E. agglomerans izolatlarında ise tikarisiline (%20) olarak belirlenmiştir.In this study, Aeromonas spp., Pesudomonas spp. and from the family of Enterobacteriaceae, E.coli, K. pneumoniae, C. freundii, P. vulgaris, E. agglomerans were isolated and identified from fish (skin, gill, intestine), water and sediment samples collected from the rivers Kızılırmak and Sakarya around Ankara (Turkey) and tested for sensitivity to antibiotics. The rate of dispersion of total 116 gram (-) islolated bacteria is identified as 37,9%, Aeromonas spp., 17,2% Pseudomonas spp., 15,5% E. coli, 10,3% K.pneumoniae, 7,7% C. freundii, 6,8% P. vulgaris and 4,3% E. agglomerans. The dispersion of antibiotic resistances obtained by disk difusion and microdilution methods are identified respectively, for Aeromonas strains ticarcillin (54,4%) and amikacin (22,8%) – (0%), for P. aeruginosa strains tetracycline (71,4%) – (57,1%) and ticarcillin (42,9%), for other Pseudomonas strains tetracycline (69,3%) – (30,7%), for E. coli strains tikarcillin (83,4%) and gentamicin (50,1%) – (11,1%), for K. pneumoniae strains tikarcillin (50%), cefotaxime (41,6%) – (16,7%), ciprofloxacin (91,7%) – (16,7%), amikacin (25%) – (8,3%), chloramphenicol (83,4%) - (16,7%), tetracycline (100%) – (100%), ceftazidime (100%) – (0%) ve imipenem (33,3%) – (0%), for C. freundii strains tetracycline (44,5%) – (0%) ve ceftazidime (66,7%) – (11,1%), for P. vulgaris strains tikarcillin (25%), amikacin (50%) – (12,5%) and tetracycline (100%) – (100%), for E. agglomerans strains tikaricillin (20%)