Congenital Zika syndrome is associated with maternal protein malnutrition

MRC Zika Rapid Response Grant (MC_PC_15102), FAPERJ (E_03/2017E_03/2017), the Brazilian Ministry of Health (DSAST/SVS 25380.001612/2017-70), and the AAPA Professional Development GrantFederal University of Rio de Janeiro. Institute of Biomedical Sciences. Rio de Janeiro, RJ, Brazil / Institute for Studies in Neuroscience and Complex Systems. Buenos Aires, Argentina.Federal University of Rio de Janeiro. Institute of Biology. Department of Genetics. Rio de Janeiro, RJ, Brazil.Federal University of Rio de Janeiro. Institute of Biology. Department of Genetics. Rio de Janeiro, RJ, Brazil.Federal University of Rio de Janeiro. Microbiology Institute Paulo de Góes. Rio de Janeiro, RJ, Brazil.Federal University of Pará. Biological Science Institute. Belém, PA, Brazil.Federal University of Rio de Janeiro. Nuclear Instrumentation Laboratory. Rio de Janeiro RJ, Brazil.Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Rio de Janeiro, RJ, Brazil.Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Rio de Janeiro, RJ, Brazil.Federal University of Rio de Janeiro. Microbiology Institute Paulo de Góes. Rio de Janeiro, RJ, Brazil.Federal University of Rio de Janeiro. Institute of Biology. Department of Genetics. Rio de Janeiro, RJ, Brazil.National Center of Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações tecnológicas. Ananindeua, PA, Brasil.State Institute of Brain Paulo Niemeyer. Rio de Janeiro, RJ, Brazil.Federal University of Rio de Janeiro. Institute of Biology. Department of Genetics. Rio de Janeiro, RJ, Brazil.Institute for Studies in Neuroscience and Complex Systems. Buenos Aires, Argentina.Fundação Oswaldo Cruz. Oswaldo Cruz Institute. Rio de Janeiro, RJ, Brazil.Harvard T. H. Chan School of Public Health. Department of Global Health and Population. Boston, MA, USA.Zika virus (ZIKV) infection during pregnancy is associated with a spectrum of developmental impairments known as congenital Zika syndrome (CZS). The prevalence of this syndrome varies across ZIKV endemic regions, suggesting that its occurrence could depend on cofactors. Here, we evaluate the relevance of protein malnutrition for the emergence of CZS. Epidemiological data from the ZIKV outbreak in the Americas suggest a relationship between undernutrition and cases of microcephaly. To experimentally examine this relationship, we use immunocompetent pregnant mice, which were subjected to protein malnutrition and infected with a Brazilian ZIKV strain. We found that the combination of protein restriction and ZIKV infection leads to severe alterations of placental structure and embryonic body growth, with offspring displaying a reduction in neurogenesis and postnatal brain size. RNA-seq analysis reveals gene expression deregulation required for brain development in infected low-protein progeny. These results suggest that maternal protein malnutrition increases susceptibility to CZS

Efeito da pressão antrópica sobre igarapés na Reserva Florestal Adolpho Ducke, área de floresta na Amazônia Central

Foram investigadas variáveis físico-químicas e químicas de ambientes aquáticos, em área de floresta primária de terra firme, próxima à área urbana, no município de Manaus. Os cursos de águas superficiais investigados drenam tanto área de floresta primária como urbanizada e, na região, são chamados igarapés. Dois desses igarapés têm suas nascentes na área urbana, adentram a área de floresta e lá se juntam. Ainda dentro da mesma área o igarapé resultante encontra-se com um outro que drena apenas área de floresta primária. Neste estudo foram pesquisadas as variáveis ambientais: pH, condutividade elétrica da água, os cátions (Na+, K+, Ca2+, Mg2+ e Fe2+) e material em suspensão. Foi possível observar diferenças significativas nas médias da concentração de íons hidrogênio, na condutividade elétrica e na quantidade de material em suspensão, entre os igarapés estudados. No igarapé cuja nascente encontra-se dentro da reserva, os valores médios correspondentes ao pH, condutividade elétrica e material em suspensão foram, respectivamente, 4,47; 6,44 mS cm-1 e 1,25 mg L-1; e os valores mais elevados registrados nos impactados foram 6,84, 141,50 mS cm-1 e 9,50 mg L-1. Os resultados mostram que o igarapé que drena área de floresta mantém suas características naturais por estar protegido das atividades antrópicas, e os que provêm da área urbana encontram-se impactados.Physicochemical and chemical variables from water environments were investigated in an area of upland primary forest, near an urban area, in the municipality of Manaus. The investigated surface water streams drain both primary forest and urbanized areas, and are called igarapés in the region. The headwaters of two of these streams are in the urban area, and they run to the forest area and join to make up another stream, and later are joined with another stream which headwaters are located inside the primary forest. This study encompassed the following environmental variables: pH, water electrical conductivity, Na+, K+, Ca2+, Mg2+ and Fe2+ cations and suspended material. It was possible to see significant differences in the concentration of hydrogen ions, electrical conductivity and in the suspended material content, among the studied streams. In the stream in which the headwaters are within the reserve, the mean values corresponding to pH, electrical conductivity and suspended material were 4.47; 6.44 mS cm-1 and 1.25 mg L-1, respectively; and the highest values found in the impacted ones were 6.84, 141.50 mS cm-1 and 9.50 mg L-1. The results show that the stream which drains the forest area maintains its natural characteristics as it is protected from human activities, and the ones which come from the urban area are impacted

Data from: Evolution and epidemic spread of SARS-CoV-2 in Brazil

Brazil currently has one of the fastest growing SARS-CoV-2 epidemics in the world. Owing to limited available data, assessments of the impact of non-pharmaceutical interventions (NPIs) on virus spread remain challenging. Using a mobility-driven transmission model, we show that NPIs reduced the reproduction number from >3 to 1–1.6 in São Paulo and Rio de Janeiro. Sequencing of 427 new genomes and analysis of a geographically representative genomic dataset identified >100 international virus introductions in Brazil. We estimate that most (76%) of the Brazilian strains fell in three clades that were introduced from Europe between 22 February11 March 2020. During the early epidemic phase, we found that SARS-CoV-2 spread mostly locally and within-state borders. After this period, despite sharp decreases in air travel, we estimated multiple exportations from large urban centers that coincided with a 25% increase in average travelled distances in national flights. This study sheds new light on the epidemic transmission and evolutionary trajectories of SARS-CoV-2 lineages in Brazil, and provide evidence that current interventions remain insufficient to keep virus transmission under control in the country

Evolution and epidemic spread of SARS-CoV-2 in Brazil

Detailed metadata on all 1,182 sequences used in this study. File contains information on epidemiology, demography, location, diagnostics, sequencing statistics and evolution of 427 SARS-CoV-2 sequences generated in this study and 755 sequences downloaded from GISAID