Genetic Basis for \u3cem\u3eRhizobium etli\u3c/em\u3e CE3 O-Antigen O-Methylated Residues That Vary According to Growth Conditions

The Rhizobium etli CE3 O antigen is a fixed-length heteropolymer with O methylation being the predominant type of sugar modification. There are two O-methylated residues that occur, on average, once per complete O antigen: a multiply O-methylated terminal fucose and 2-O methylation of a fucose residue within a repeating unit. The amount of the methylated terminal fucose decreases and the amount of 2-O-methylfucose increases when bacteria are grown in the presence of the host plant, Phaseolus vulgaris, or its seed exudates. Insertion mutagenesis was used to identify open reading frames required for the presence of these O-methylated residues. The presence of the methylated terminal fucose required genes wreA, wreB, wreC, wreD, and wreF, whereas 2-O methylation of internal fucoses required the methyltransferase domain of bifunctional gene wreM. Mutants lacking only the methylated terminal fucose, lacking only 2-O methylation, or lacking both the methylated terminal fucose and 2-O methylation exhibited no other lipopolysaccharide structural defects. Thus, neither of these decorations is required for normal O-antigen length, transport, or assembly into the final lipopolysaccharide. This is in contrast to certain enteric bacteria in which the absence of a terminal decoration severely affects O-antigen length and transport. R. etli mutants lacking only the methylated terminal fucose were not altered in symbiosis with host Phaseolus vulgaris, whereas mutants lacking only 2-O-methylfucose exhibited a delay in nodule development during symbiosis. These results support previous conclusions that the methylated terminal fucose is dispensable for symbiosis, whereas 2-O methylation of internal fucoses somehow facilitates early events in symbiosis

Economic analysis of landholder water management under Cap & Pipe the Bores program: case studies in the NSW Great Artesian Basin

The NSW Cap & Pipe the Bores Program is a jointly funded Commonwealth and State initiative that operates within the Australian Government’s Great Artesian Basin Sustainability Initiative (GABSI) framework. The third five-year period of GABSI was due to commence on 1st July 2009. This economic study of changes in landholder water management under the Cap & Pipe the Bores program was undertaken to inform the development of GABSI phase 3 policy in NSW. The study consisted of a small number of case studies of farming enterprises across the Great Artesian Basin Groundwater Management Zones of Surat South, Surat East, Surat West, Warrego and Central in NSW. The apparent landholder economic feasibility of the program was dependent on individual farm characteristics as much as location to the bore and geographic location within the GAB.Resource /Energy Economics and Policy,

NSW Great Artesian Basin water market assessment pre and post auction

The NSW Cap & Pipe the Bores Program is a jointly funded Commonwealth and State initiative that operates within the Australian Government's Great Artesian Basin Sustainability Initiative framework. Under the Water Sharing Plan for the NSW Great Artesian Basin Groundwater Sources 2008, 70 per cent of the water savings made since 1999 shall be retained in the aquifers of the Basin to improve pressures and provide for groundwater dependent ecosystems. The remaining 30 per cent of the water savings may be released to extractive users over the term of the Water Sharing Plan to facilitate the continued economic and social welfare of regional western NSW. As the first step in this water release, part of the water savings from the Cap & Pipe the Bores program were auctioned in July 2009 in the western NSW town of Walgett. All lots offered at auction were sold. The study reviews the pre auction analysis of demand, and outlines the auction process and outcomes as well as the implications for future water sales. The outcomes of the auction will inform ongoing water allocation policy.water auction, economics, Great Artesian Basin, groundwater, Cap & Pipe the Bores, Water Sharing Plan., Resource /Energy Economics and Policy,

Advanced content-based semantic scene analysis and information retrieval: the SCHEMA project

The aim of the SCHEMA Network of Excellence is to bring together a critical mass of universities, research centers, industrial partners and end users, in order to design a reference system for content-based semantic scene analysis, interpretation and understanding. Relevant research areas include: content-based multimedia analysis and automatic annotation of semantic multimedia content, combined textual and multimedia information retrieval, semantic -web, MPEG-7 and MPEG-21 standards, user interfaces and human factors. In this paper, recent advances in content-based analysis, indexing and retrieval of digital media within the SCHEMA Network are presented. These advances will be integrated in the SCHEMA module-based, expandable reference system

Does the Sun shrink with increasing magnetic activity?

It has been demonstrated that frequencies of f-modes can be used to estimate the solar radius to a good accuracy. These frequencies have been used to study temporal variations in the solar radius with conflicting results. The variation in f-mode frequencies is more complicated than what is assumed in these studies. If a careful analysis is performed then it turns out that there is no evidence for any variation in the solar radius.Comment: To appear in Astrophys.

FADTTSter: Accelerating hypothesis testing with functional analysis of diffusion tensor tract statistics

Functional Analysis of Diffusion Tensor Tract Statistics (FADTTS) is a toolbox for analysis of white matter (WM) fiber tracts. It allows associating diffusion properties along major WM bundles with a set of covariates of interest, such as age, diagnostic status and gender, and the structure of the variability of these WM tract properties. However, to use this toolbox, a user must have an intermediate knowledge in scripting languages (MATLAB). FADTTSter was created to overcome this issue and make the statistical analysis accessible to any non-technical researcher. FADTTSter is actively being used by researchers at the University of North Carolina. FADTTSter guides non-technical users through a series of steps including quality control of subjects and fibers in order to setup the necessary parameters to run FADTTS. Additionally, FADTTSter implements interactive charts for FADTTS' outputs. This interactive chart enhances the researcher experience and facilitates the analysis of the results. FADTTSter's motivation is to improve usability and provide a new analysis tool to the community that complements FADTTS. Ultimately, by enabling FADTTS to a broader audience, FADTTSter seeks to accelerate hypothesis testing in neuroimaging studies involving heterogeneous clinical data and diffusion tensor imaging. This work is submitted to the Biomedical Applications in Molecular, Structural, and Functional Imaging conference. The source code of this application is available in NITRC

Evaluation of the cancer risk from PAHs by inhalation:Are current methods fit for purpose?

There is ample evidence from occupational studies that exposure to a mixture of Polycyclic Aromatic Hydrocarbons (PAHs) is causally associated with an increased incidence of lung cancers. In both occupational atmospheres and ambient air, PAHs are present as a mixture of many compounds, but the composition of the mixture in ambient air differs from that in the occupational atmosphere, and varies in time and space in ambient air. Estimates of cancer risk for PAH mixtures are based upon unit risks which derive from extrapolation of occupational exposure data or animal model data, and in the case of the WHO use one compound, benzo[a]pyrene as a marker for the entire mixture, irrespective of composition. The U.S. EPA has used an animal exposure study to derive a unit risk for inhalation exposure to benzo[a]pyrene alone, and there have been a number of rankings of relative carcinogenic potency for other PAHs which many studies have used to calculate a cancer risk from the PAHs mixture, frequently incorrectly by adding the estimated relative risks of individual compounds, and applying the total “B[a]P equivalent” to the WHO unit risk, which already applies to the entire mixture. Such studies are often based upon data solely for the historic US EPA group of 16 compounds which do not include many of the apparently more potent carcinogens. There are no data for human cancer risk of individual PAHs, and conflicting evidence of additivity of PAH carcinogenicity in mixtures. This paper finds large divergences between risk estimates deriving from the WHO and U.S. EPA methods, as well as considerable sensitivity to the mixture composition, and assumed PAH relative potencies. Of the two methods, the WHO approach appears more likely to provide reliable risk estimates, but recently proposed mixture-based approaches using in vitro toxicity data may offer some advantages.peer-reviewe