Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates

Supplemental information for this article can be found online at http://dx.doi.org/10.7717/peerj.9549#supplemental-information.Staphylococcus epidermidis is one of the major opportunistic bacterial pathogens in healthcare facilities, mainly due to its strong ability to form biofilms in the surface of indwelling medical devices. To study biofilms under in vitro conditions, both fed-batch and flow systems are widely used, with the first being the most frequent due to their low cost and ease of use. Aim To assess if a fed-batch system previously developed to obtain biofilm released cells (Brc) from strong biofilm producing S. epidermidis isolates could also be used to obtain and characterize Brc from isolates with lower abilities to form biofilms. Methodology The applicability of a fed-batch system to obtain Brc from biofilms of 3 ica+ and 3 ica isolates was assessed by quantifying the biofilm and Brc biomass by optical density (OD) and colony-forming units (CFU) measurements. The effect of media replacement procedures of fed-batch systems on the amount of biofilm was determined by quantifying the biofilm and biofilm bulk fluid, by CFU, after consecutive washing steps. Results The fed-batch model was appropriate to obtain Brc from ica+ isolates, that presented a greater ability to form biofilms and release cells. However, the same was not true for ica isolates, mainly because the washing procedure would physically remove a significant number of cells from the biofilm. Conclusions This study demonstrates that a fed-batch system is only feasible to be used to obtain Brc from S. epidermidis when studying strong and cohesive biofilm-forming isolates.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 and by BioTecNorteoperation (NORTE-01-0145-FEDER-000004) funded by European Regional DevelopmentFund under the scope of Norte2020 - Programa Operacional Regional do Norte. Thefunders had no role in study design, data collection and analysis, decision to publish, orpreparation of the manuscript.info:eu-repo/semantics/publishedVersio

Information Systems Management in Perspective of Business Benefits

This paper describes possible way of building an effective IT strategy in perspective of long-term business benefits in order to size of the firm, branch and the type of business. Firstly the general scope of this way is given and the second part the case connected with building an appropriate IT strategy for economical High School is described as an example.Zadanie pt. „Digitalizacja i udostępnienie w Cyfrowym Repozytorium Uniwersytetu Łódzkiego kolekcji czasopism naukowych wydawanych przez Uniwersytet Łódzki” nr 885/P-DUN/2014 zostało dofinansowane ze środków MNiSW w ramach działalności upowszechniającej nauk

Modeling and Analysis of Scholar Mobility on Scientific Landscape

Scientific literature till date can be thought of as a partially revealed landscape, where scholars continue to unveil hidden knowledge by exploring novel research topics. How do scholars explore the scientific landscape , i.e., choose research topics to work on? We propose an agent-based model of topic mobility behavior where scholars migrate across research topics on the space of science following different strategies, seeking different utilities. We use this model to study whether strategies widely used in current scientific community can provide a balance between individual scientific success and the efficiency and diversity of the whole academic society. Through extensive simulations, we provide insights into the roles of different strategies, such as choosing topics according to research potential or the popularity. Our model provides a conceptual framework and a computational approach to analyze scholars' behavior and its impact on scientific production. We also discuss how such an agent-based modeling approach can be integrated with big real-world scholarly data.Comment: To appear in BigScholar, WWW 201

Organizing Dark Matter: W.A.G.E. as Alternative Worker Organization

Since its founding in 2008, W.A.G.E. (Working Artists and the Greater Economy) has worked to reform the economic habits of US art institutions and of the artists upon whose cultural work these institutions are dependent. Inside a decade, W.A.G.E. went from a small grassroots collective to an internationally recognized, yet lean, organization, which not only advocates for labour standards in the nonprofit art sector, but also develops practical tools to begin the work of doing better by equality in the art world. This chapter positions W.A.G.E. as an example of what Immanuel Ness terms “new forms of worker organization.” Informed by W.A.G.E.-authored texts, media coverage of W.A.G.E., and interviews with the group’s core organizer and programmer, the chapter surveys W.A.G.E.’s strategies for organizing “dark matter,” a concept that Gregory Sholette has repurposed from physics as a metaphor for the majority of artists and activities that populate the art world and uphold and subsidize its most visible and commercially successful figures. W.A.G.E. is explored in five registers: its practice of parrhesia, algorithm of fairness, strategy of certification, post-horizontalist form of organization, and platformization of labour politics. While W.A.G.E. has been tackling dilemmas specific to the nonprofit arts, its strategies hold wider relevance to confronting the challenge of organizing workers who are outside of an employment relationship, who lack access to unions, and for whom the opportunity to be self-expressive or the promise of exposure may be regarded as compensation enough

The interrelationship between the demands of Corporate Social Responsibility and co-operative principles and values

Corporate Social Responsibility (CSR) and its integrated management in companies is leading to a new company model that willingly expands its concerns by giving equal consideration to economic, social and environmental aspects. Cooperative societies, in paying attention to the principles and values that define them, as shown in this work, are demonstrating that there is a close interrelationship with the basic demands of CSR, which can be a competitive advantage as long as it is driven by a strategy that leads to its integrated management. The singular structure and democratic composition of a cooperative’s management structure can guarantee the total integration of stakeholders in the decision-making process, as they are often not only owners, but also clients, suppliers and employees.Corporate Social Responsibility, management, interest groups, cooperatives, stakeholders.

The FairShares Model: a communitarian pluralist approach to constituting social enterprises?

Objectives - This paper is an exploration of the intellectual antecedents and philosophical assumptions that underpin the FairShares Model - a set of brand principles and Articles of Association published by the FairShares Association. It contributes to knowledge of the history of the social enterprise movement and its link to contemporary developments in mutual social enterprises. Prior Work - Previous contributions to the literature on social economy have drawn on communitarian philosophy to develop insights into mutual principles. This paper sets out a theoretical framework to evaluate whether the FairShares Model represents a communitarian pluralist discourse on the constitution of social enterprises. Approach - In January 2013, the FairShares Association published guidance on the FairShares 'brand' and 'model' (drawing on work presented at ISBE) to develop the concept of a ‘socialised’ enterprise . The framework developed from prior work is used to assess which aspects of communitarian philosophy are emphasized in both antecedent model rules (identified by the FairShares Association) as well as the FairShares Model (v1.2a). Results - The FairShares Model is theorised as a predominantly communitarian pluralist discourse with some ‘corporatist’ commitments. It represents an evolving set of guidelines for the ‘socialisation’ of enterprise by devising membership rights for two primary stakeholders (labour, users), and two secondary stakeholders (founders, investors). It is designed to reverse the centralising and accumulating tendencies of the private sector without returning assets to state control. It differs from philanthropic models by offering co-operative (par value) shares to three member classes: founders, labour and users, and (ordinary) ‘investor’ shares to all classes of member. Implications - The FairShares Model contributes to knowledge on the 'socialisation' of enterprise by identifying core characteristics of member-owned enterprises that deploy strategies for multi stakeholder ownership, governance and management. Value – By operationalising a communitarian pluralist discourse in the process of constituting a social enterprise, the FairShares Model offers an alternative to private sector models based on the subordination of labour and mutual models based on the primacy of a single stakeholder group