Growth and thermal stability of TiN/ZrAlN: Effect of internal interfaces

Wear resistant hard films comprised of cubic transition metal nitride (c-TMN) and metastable c-AlN with coherent interfaces have a confined operating envelope governed by the limited thermal stability of metastable phases. However, equilibrium phases (c-TMN and wurtzite(w)-AlN) forming semicoherent interfaces during film growth offer higher thermal stability. We demonstrate this concept for a model multilayer system with TiN and ZrAlN layers where the latter is a nanocomposite of ZrN- and AlN- rich domains. The interfaces between the domains are tuned by changing the AlN crystal structure by varying the multilayer architecture and growth temperature. The interface energy minimization at higher growth temperature leads to formation of semicoherent interfaces between w-AlN and c-TMN during growth of 15 nm thin layers. Ab initio calculations predict higher thermodynamic stability of semicoherent interfaces between c-TMN and w-AlN than isostructural coherent interfaces between c-TMN and c-AlN. The combination of a stable interface structure and confinement of w-AlN to nm-sized domains by its low solubility in c-TMN in a multilayer, results in films with a stable hardness of 34 GPa even after annealing at 1150 °C.Peer ReviewedPostprint (author's final draft

Clonal hematopoiesis and therapy-related myeloid neoplasms following neuroblastoma treatment.

Therapy-related myeloid neoplasms (TMN) constitute one of the most challengingcomplications of cancer treatment.1 Whilst understanding of TMN pathogenesis remains fragmentary, genomic studies in adults have thus far refuted the notion that TMN simply result from cytotoxin-induced DNA damage.2–4 Analysis of the preclinical evolution of a limited number of adult TMN have retraced the majority of cases to clonal haematopoiesis (CH) that predates cytotoxic treatment and lacks the mutational footprint of genotoxic therapies.2–6 Balanced translocations, generally attributed to treatment with topoisomerase II inhibitors, are implicated in a minority of TMN.1 TMN is a leading cause of premature death in childhood cancer survivors, and affects 7-11% of children treated for high-risk neuroblastoma and sarcoma.7,8 However, the origin of pediatric TMN remains unclear. Targeted sequencing of known cancer genes detects CH in ~4% of children following cytotoxic treatment,6,9 whereas CH is vanishingly rare in young individuals in the general population.10,11 Moreover, to our knowledge, no cases of childhood TMN have been retraced to pretreatment CH. In light of these observations, we asked whether a broader driver landscape had eluded targeted CH screens in pediatric cancer patients and/or whether therapy-induced mutagenesis may be an under-recognised catalyst of CH and TMN in this patient group

The Value of Citizen Scientists: Data Collection for American Eel Using Non-Traditional Field Gear & Social Media

American Eel (Anguilla rostrata) is a facultative catadromous species with a unique and complex life history. After hatching, larval eel begin their journey as leptocephalus in the Sargasso Sea and drift on ocean currents along the Atlantic coast, Gulf of Mexico, and Central and South America. They transform into glass eel as they approach shore and begin to develop pigment as they settle in estuaries or move upstream into rivers as elvers. American Eel then spend 3-40+ years in these habitats as yellow eel until they sexually mature into silver eel and return to the Sargasso Sea where they spawn and presumably die. State and federal agencies, multiple universities and numerous citizen science volunteers are working to better understand their movement patterns and recruitment window in Texas. Citizen scientists with coastal chapters of the Texas Master Naturalists (TMN) have taken a lead role in assisting with this effort. Since February of 2018, TMN have established a network of monitoring sites across the mid to upper Texas Coast to sample for juvenile American Eel using eel mops. Eel mops have been deployed for various lengths of time at 29 sites throughout the past two years and checked routinely for glass and elver eel. Volunteers have conducted approximately 250 eel mop checks and provided record of their catch by category (e.g., eel, shrimp, crab, other fish, etc.) based on occurrence or abundance. TMN have documented close to 7,000 individuals across all categories with various species of crab, shrimp, and fish being the most common groups collected. While no glass or elver eel have been collected in an eel mop, TMN have provide valuable data for this project by testing a common gear type that is often used to monitor for American Eel on the Atlantic Coast.Integrative Biolog

Reassessing the Role of Histaminergic Tuberomammillary Neurons in Arousal Control

The histaminergic neurons of the tuberomammillary nucleus (TMN(HDC)) of the posterior hypothalamus have long been implicated in promoting arousal. More recently, a role for GABAergic signaling by the TMN(HDC) neurons in arousal control has been proposed. Here, we investigated the effects of selective chronic disruption of GABA synthesis (via genetic deletion of the GABA synthesis enzyme, glutamic acid decarboxylase 67) or GABAergic transmission (via genetic deletion of the vesicular GABA transporter (VGAT)) in the TMN(HDC) neurons on sleep-wake in male mice. We also examined the effects of acute chemogenetic activation and optogenetic inhibition of TMN(HDC) neurons upon arousal in male mice. Unexpectedly, we found that neither disruption of GABA synthesis nor GABAergic transmission altered hourly sleep-wake quantities, perhaps because very few TMN(HDC) neurons coexpressed VGAT. Acute chemogenetic activation of TMN(HDC) neurons did not increase arousal levels above baseline but did enhance vigilance when the mice were exposed to a behavioral cage change challenge. Similarly, acute optogenetic inhibition had little effect upon baseline levels of arousal. In conclusion, we could not identify a role for GABA release by TMN(HDC) neurons in arousal control. Further, if TMN(HDC) neurons do release GABA, the mechanism by which they do so remains unclear. Our findings support the view that TMN(HDC) neurons may be important for enhancing arousal under certain conditions, such as exposure to a novel environment, but play only a minor role in behavioral and EEG arousal under baseline conditions.SIGNIFICANCE STATEMENT The histaminergic neurons of the tuberomammillary nucleus of the hypothalamus (TMN(HDC)) have long been thought to promote arousal. Additionally, TMN(HDC) neurons may counter-regulate the wake-promoting effects of histamine through co-release of the inhibitory neurotransmitter, GABA. Here, we show that impairing GABA signaling from TMN(HDC) neurons does not impact sleep-wake amounts and that few TMN(HDC) neurons contain the vesicular GABA transporter, which is presumably required to release GABA. We further show that acute activation or inhibition of TMN(HDC) neurons has limited effects upon baseline arousal levels and that activation enhances vigilance during a behavioral challenge. Counter to general belief, our findings support the view that TMN(HDC) neurons are neither necessary nor sufficient for the initiation and maintenance of arousal under baseline conditions

Tree Memory Networks for Modelling Long-term Temporal Dependencies

In the domain of sequence modelling, Recurrent Neural Networks (RNN) have been capable of achieving impressive results in a variety of application areas including visual question answering, part-of-speech tagging and machine translation. However this success in modelling short term dependencies has not successfully transitioned to application areas such as trajectory prediction, which require capturing both short term and long term relationships. In this paper, we propose a Tree Memory Network (TMN) for modelling long term and short term relationships in sequence-to-sequence mapping problems. The proposed network architecture is composed of an input module, controller and a memory module. In contrast to related literature, which models the memory as a sequence of historical states, we model the memory as a recursive tree structure. This structure more effectively captures temporal dependencies across both short term and long term sequences using its hierarchical structure. We demonstrate the effectiveness and flexibility of the proposed TMN in two practical problems, aircraft trajectory modelling and pedestrian trajectory modelling in a surveillance setting, and in both cases we outperform the current state-of-the-art. Furthermore, we perform an in depth analysis on the evolution of the memory module content over time and provide visual evidence on how the proposed TMN is able to map both long term and short term relationships efficiently via a hierarchical structure

Applying Service Engineering Principles to TMN Systems

Recent work in distributed and telecommunication systems has become increasingly oriented towards applications in a global open market in telecommunications services, as the effects of liberalisation take hold. The areas of network and service management will be essential to competitiveness in this market, however the technologies and system development techniques widely used today (i.e. SNMP and CMIP) were conceived to address the needs of large corporate data networks and monolithic public telecommunication networks. How these technologies and techniques can be applied to management in an open market environment is not currently well defined. This paper describes some of the work of the RACE II PROJECT PREPARE in its application of ITU-T TMN recommendations to management solutions in an open services environment. In particular it describes the engineering approach taken to developing TMN systems in an attempt to tailor them to such an environment

Massless scalar fields and infrared divergences in the inflationary brane world

We study the quantum effects induced by bulk scalar fields in a model with a de Sitter (dS) brane in a flat bulk (the Vilenkin-Ipser-Sikivie model) in more than four dimensions. In ordinary dS space, it is well known that the stress tensor in the dS invariant vacuum for an effectively massless scalar (m_\eff^2=m^2+\xi {\cal R}=0 with ${\cal R}$ the Ricci scalar) is infrared divergent except for the minimally coupled case. The usual procedure to tame this divergence is to replace the dS invariant vacuum by the Allen Follaci (AF) vacuum. The resulting stress tensor breaks dS symmetry but is regular. Similarly, in the brane world context, we find that the dS invariant vacuum generates \tmn divergent everywhere when the lowest lying mode becomes massless except for massless minimal coupling case. A simple extension of the AF vacuum to the present case avoids this global divergence, but \tmn remains to be divergent along a timelike axis in the bulk. In this case, singularities also appear along the light cone emanating from the origin in the bulk, although they are so mild that \tmn stays finite except for non-minimal coupling cases in four or six dimensions. We discuss implications of these results for bulk inflaton models. We also study the evolution of the field perturbations in dS brane world. We find that perturbations grow linearly with time on the brane, as in the case of ordinary dS space. In the bulk, they are asymptotically bounded.Comment: 20 pages. References adde

Bifurcation of Fredholm maps II; The dimension of the set of bifurcation points

We obtain an estimate for the covering dimension of the set of bifurcation points for solutions of nonlinear elliptic boundary value problems from the principal symbol of the linearization of the problem along the trivial branch of solutions.Comment: 15 pages, corrected typos, minor changes; La Matematica e le sue Applicazioni N5(2010). To appear on TMN

The Possible Role of TASK Channels in Rank-Ordered Recruitment of Motoneurons in the Dorsolateral Part of the Trigeminal Motor Nucleus.

Because a rank-ordered recruitment of motor units occurs during isometric contraction of jaw-closing muscles, jaw-closing motoneurons (MNs) may be recruited in a manner dependent on their soma sizes or input resistances (IRs). In the dorsolateral part of the trigeminal motor nucleus (dl-TMN) in rats, MNs abundantly express TWIK (two-pore domain weak inwardly rectifying K channel)-related acid-sensitive-K(+) channel (TASK)-1 and TASK3 channels, which determine the IR and resting membrane potential. Here we examined how TASK channels are involved in IR-dependent activation/recruitment of MNs in the rat dl-TMN by using multiple methods. The real-time PCR study revealed that single large MNs (>35 μm) expressed TASK1 and TASK3 mRNAs more abundantly compared with single small MNs (15-20 μm). The immunohistochemistry revealed that TASK1 and TASK3 channels were complementarily distributed in somata and dendrites of MNs, respectively. The density of TASK1 channels seemed to increase with a decrease in soma diameter while there were inverse relationships between the soma size of MNs and IR, resting membrane potential, or spike threshold. Dual whole-cell recordings obtained from smaller and larger MNs revealed that the recruitment of MNs depends on their IRs in response to repetitive stimulation of the presumed Ia afferents. 8-Bromoguanosine-cGMP decreased IRs in small MNs, while it hardly changed those in large MNs, and subsequently decreased the difference in spike-onset latency between the smaller and larger MNs, causing a synchronous activation of MNs. These results suggest that TASK channels play critical roles in rank-ordered recruitment of MNs in the dl-TMN