What’s a Guanaco?: Tracing the Llama Diaspora Through and Beyond South America

Let us begin with Spook the llama. Spook lived in the animal enclosures at New York’s Central Park Zoo in 1912. Caretakers described Spook as a “morose, cantankerous” soul inhabiting the back of the deer range. Initially forlorn by this location, he looked out the back door at the road that circled past the pen. The busy street proved entertaining as it was full of noisy automobiles and anxious drivers honking. Spook watched the cars and, before long, learned to honk. Or so reported the head keeper at the zoo, Bill Snyder, who claimed “Spook thrust his head forward, drew back his lips so that his teeth were showing, and made a low and distinct sound like an automobile horn.” Spook responded to honks, instigated them, and generally wreaked havoc distracting drivers with his uncanny honking. Complaints mounted and Snyder moved Spook upstate to a pasture along the river. Rather than live out his days peacefully, Spook observed the ships and soon learned to imitate boat whistles, restarting this cycle of imitation

An analysis of the McKee phonetic inventory for grade three.

Thesis (Ed.M.)--Boston Universit

Deoxyribonucleases expressed in competence-induced com and spo0 mutants of Bacillus subtilis

The inclusion of 1% casein in buffer used to reactivate enzymes subjected to SDS-polyacrylamide electrophoresis resulted in faster and more complete restoration of nuclease and B-galactosidase enzyme activities. Enzyme activities which were absent from gels during longer wash procedures were detectable with this technique. The threshold of detection of two-dimensionally separated Deoxyribonuclease I was 1 picogram, tenfold lower than for previously reported wash procedures. Addition of BSA at concentrations above 50 ug/ml to nuclease gels was found to result in less effective detergent removal during wash procedures and reduced recovery of enzyme activity. General and specific nuclease expression patterns were observed in two-dimensional sodium dodecyl sulfate polyacrylamide gels of 64 competence-induced com and spo mutants of Bacillus subtilis. General patterns of nucleases expression were as follows: (1) Four different groups of nucleases called clusters were found to be co-expressed in greater than 95% of the mutants. (2) Some cluster members are probably produced by post-translational modification. (3) A 14 kd nuclease reported by Venema was detected in the gels after use of the casein wash procedure. Specific patterns of expression are as follows: (1) Sigma factor H is not required for nuclease 2 expression. (2) spoOK is required for nuclease 2 production. (3) Nuclease 2 is regulated differently than nuclease clusters A, B, C, and D. (4) crsa\sb{47} (sigma A) is required for competence

Hoop Houses in Rural Alaska: Twenty Questions and Answers to Get You Started

This publication addresses the most common questions people have when considering whether to build a hoop house. There is information on the sizes and shapes of hoop houses, the cost of building and/or shipping a hoop house, ease of construction, sunlight and heat requirements, advice on what kind of production to expect, and much more.For more information, contact your local Cooperative Extension Service office or Jeff Smeenk, Extension Horticulture Specialist, Agriculture and Horticulture, at 907-746-9470 or [email protected]. This publication was reviewed by Stephen Brown, Extension Faculty, Heidi Rader, Tribes Extension Educator, and Kendra Calhoun, Extension Instructor