25,787 research outputs found
Nanodiamond landmarks for subcellular multimodal optical and electron imaging.
There is a growing need for biolabels that can be used in both optical and electron microscopies, are non-cytotoxic, and do not photobleach. Such biolabels could enable targeted nanoscale imaging of sub-cellular structures, and help to establish correlations between conjugation-delivered biomolecules and function. Here we demonstrate a sub-cellular multi-modal imaging methodology that enables localization of inert particulate probes, consisting of nanodiamonds having fluorescent nitrogen-vacancy centers. These are functionalized to target specific structures, and are observable by both optical and electron microscopies. Nanodiamonds targeted to the nuclear pore complex are rapidly localized in electron-microscopy diffraction mode to enable "zooming-in" to regions of interest for detailed structural investigations. Optical microscopies reveal nanodiamonds for in-vitro tracking or uptake-confirmation. The approach is general, works down to the single nanodiamond level, and can leverage the unique capabilities of nanodiamonds, such as biocompatibility, sensitive magnetometry, and gene and drug delivery
Towards an Efficient Use of the BLAS Library for Multilinear Tensor Contractions
Mathematical operators whose transformation rules constitute the building
blocks of a multi-linear algebra are widely used in physics and engineering
applications where they are very often represented as tensors. In the last
century, thanks to the advances in tensor calculus, it was possible to uncover
new research fields and make remarkable progress in the existing ones, from
electromagnetism to the dynamics of fluids and from the mechanics of rigid
bodies to quantum mechanics of many atoms. By now, the formal mathematical and
geometrical properties of tensors are well defined and understood; conversely,
in the context of scientific and high-performance computing, many tensor-
related problems are still open. In this paper, we address the problem of
efficiently computing contractions among two tensors of arbitrary dimension by
using kernels from the highly optimized BLAS library. In particular, we
establish precise conditions to determine if and when GEMM, the kernel for
matrix products, can be used. Such conditions take into consideration both the
nature of the operation and the storage scheme of the tensors, and induce a
classification of the contractions into three groups. For each group, we
provide a recipe to guide the users towards the most effective use of BLAS.Comment: 27 Pages, 7 figures and additional tikz generated diagrams. Submitted
to Applied Mathematics and Computatio
Digital design of medical replicas via desktop systems: shape evaluation of colon parts
In this paper, we aim at providing results concerning the application of desktop systems for rapid prototyping of medical replicas
that involve complex shapes, as, for example, folds of a colon. Medical replicas may assist preoperative planning or tutoring in
surgery to better understand the interaction among pathology and organs. Major goals of the paper concern with guiding the
digital design workflow of the replicas and understanding their final performance, according to the requirements asked by the
medics (shape accuracy, capability of seeing both inner and outer details, and support and possible interfacing with other organs).
In particular, after the analysis of these requirements, we apply digital design for colon replicas, adopting two desktop systems. ,e
experimental results confirm that the proposed preprocessing strategy is able to conduct to the manufacturing of colon replicas
divided in self-supporting segments, minimizing the supports during printing. ,is allows also to reach an acceptable level of final
quality, according to the request of having a 3D presurgery overview of the problems. ,ese replicas are compared through reverse
engineering acquisitions made by a structured-light system, to assess the achieved shape and dimensional accuracy. Final results
demonstrate that low-cost desktop systems, coupled with proper strategy of preprocessing, may have shape deviation in the range
of ±1 mm, good for physical manipulations during medical diagnosis and explanation
Manual for starch gel electrophoresis: A method for the detection of genetic variation
The procedure to conduct horizontal starch gel electrophoresis on enzymes is described in detail. Areas covered are (I) collection and storage of specimens, (2)
preparation of tissues, (3) preparation of a starch gel, (4) application of enzyme extracts to a gel, (5) setting up a gel for electrophoresis, (6) slicing a gel, and (7)
staining a gel. Recipes are also included for 47 enzyme stains and 3 selected gel buffers. (PDF file contains 26 pages.
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Converting a CAD Model into a Manufacturing Model for the Components Made of a Multiphase Perfect Material
To manufacture the component made of a multiphase perfect material (including homogeneous
and multi heterogeneous materials), it CAD model should be processed and converted into
layered manufacturing model for further transformation of numerical control (NC) coding. This
paper develops its detailed approaches and corresponding software. The process planning is made
first and includes: (1) determining the build orientation of the component; and (2) slicing the
component into layers adaptively according to different material regions since different materials
have different optimal layer thickness for manufacturing. After the process planning, the layered
manufacturing models with necessary information, including fabrication sequence and material
information of each layer, are fully generated.Mechanical Engineerin
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