PENGARUH PEMBERIAN SUPLEMENTASI SUPEROXIDE DISMUTASE (SOD) TERHADAP KADAR ALBUMIN SERUM PADA LANSIA

Background: Levels of serum albumin can be used as a predictor of morbidity and mortality in the elderly. Reduced serum albumin concentration can be caused by oxidative modification due to aging or insufficient protein intake. SOD as an enzymatic antioxidant might prevent oxidative stress so that albumin modification process can be inhibited. SOD supplementation was expected to increase serum albumin levels. Aim: Analyze the effect of SOD supplementation on elderly serum albumin level. Methods: This was a true experimental study with pre and post test control group design.. The study began with 31 elderly, resides in the “Pucang Gading Social Rehabilitation Unit”. They were divided into 2 groups. The control group (15 subjects), received placebo and exercise. The treatment group (16 subjects), received 250 IU SOD/day and exercise. Both treatments were done within 8 weeks, with twice a week exercise. Before and after treatment, levels of serum albumin were measured. Data normality was tested using Saphiro-wilk test. Data was analyzed by Paired-T-test if the distribution is normal, and using Wilcoxon test if the distribution is abnormal. Results: There were increases of serum albumin levels in both groups. Statistical test results showed a significant increase of serum albumin levels in the treatment group of 0.26 ± 0.33 mg/dL with p=0,007 (p0,05). Conclusion: 250 IU SOD/day supplementation for 8 weeks increase serum albumin levels in the elderly. Keywords: Albumin, elderly, aging, SO

Ursodeoxycholic acid improves bilirubin but not albumin in primary biliary cirrhosis: further evidence for nonefficacy.

BACKGROUND/AIM: In randomised controlled trials (RCTs) of ursodeoxycholic acid (UDCA), although serum bilirubin is frequently reduced, its effect on disease progression and mortality is unclear. As serum albumin is an established independent prognostic marker, one might expect less deterioration of serum albumin values in a UDCA-treated group. We therefore modelled the typical evolution of serum bilirubin and albumin levels over time in UDCA-untreated patients and compared it with the observed levels in UDCA RCTs. METHODS: Multilevel modelling was used to relate the evolution of serum albumin to serum bilirubin and time since patient referral. For each considered RCT, the derived model was used to predict the relationship between final mean serum albumin and bilirubin concentration, adjusted for mean serum albumin at referral and followup duration. RESULTS: Five RCTs were eligible in terms of available data, of which two had long followup. In all trials, serum albumin did not significantly differ between UDCA- and placebo-treated patients, despite the UDCA effect on serum bilirubin. Therefore, there is no evidence over time for changes or maintenance of albumin levels for UDCA-treated patients above the levels predicted for placebo-treated patients. CONCLUSIONS: Our findings suggest that UDCA does not alter serum albumin in a way that is consistent with its effect on serum bilirubin. Therefore, reductions in serum bilirubin of UDCA-treated PBC do not parallel another validated and independent prognostic marker, further questioning the validity of serum bilirubin reduction with UDCA as a surrogate therapeutic marker

Long-Range Proton Conduction Across Free-Standing Serum Albumin

Free‐standing serum‐albumin mats can transport protons over millimetre length‐scales. The results of photoinduced proton transfer and voltage‐driven proton‐conductivity measurements, together with temperature‐dependent and isotope‐effect studies, suggest that oxo‐amino‐acids of the protein serum albumin play a major role in the translocation of protons via an “over‐the‐barrier” hopping mechanism. The use of proton‐conducting protein mats opens new possibilities for bioelectronic interfaces

Sequence homology between RNAs encoding rat α-fetoprotein and rat serum albumin

We have determined the sequences of the recombinant DNA inserts of three bacterial plasmid cDNA clones containing most of the rat α-fetoprotein mRNA. The resultant nucleotide sequence of α-fetoprotein was exhaustively compared to the nucleotide sequence of the mRNA encoding rat serum albumin. These two mRNAs have extensive homology (50%) throughout and the same intron locations. The amino acid sequence of rat α-fetoprotein has been deduced from the nucleotide sequence, and its comparison to rat serum albumin's amino acid sequence reveals a 34% homology. The regularly spaced positions of the cysteines found in serum albumin are conserved in rat α-fetoprotein, indicating that these two proteins may have a similar secondary folding structure. These homologies indicate that α-fetoprotein and serum albumin were derived by duplication of a common ancestral gene and constitute a gene family

Correlation Between Serum Albumin Level and Degree of Esophageal Varices in Patients with Liver Cirrhosis

Background: It has not been clear about how often the patient should have esophago- gastroduodenoscopy (EGD) screening for esophageal varices (EV) detection and there is only some data that demonstrates the correlation between the degree of EV and non-endoscopic variables. It is assumed that the presence of EV detected though examination of serum albumin level may trim down the unnecessary endoscopy. This study was aimed to recognize the correlation between albumin level and the degree of EV in patients with liver cirrhosis. Method: A retrospective analysis was performed for 61 patients with liver cirrhosis who had EGD at Sanglah hospital between January and December 2008. Spearman test was used to analyze the correlation between albumin level and the degree of EV. Results: There were 61 patients of 45 (73.8%) male and 16 (26.2%) female. The range age of patients was 13–77 years (average 49.98 ± 1.62 years). Serum albumin level ranged between 1.10-3.60 mg/dL, the average value was 2.21 ± 0.451 mg/dL. We also found 8 (13.1%) patients without EV, 14 (23.0%) patients with EV grade I, 21 (34.4%) patients with grade II and 18 (29.5%) patients with grade III. A negative correlation was found between serum albumin level and the degree of EV (r = - 0.587; p = 0.000, p < 0.01). Conclusion: Serum albumin level can predict the presence and the degree of EV in patients with liver cirrhosis

<Originals> Studies on Quantitative Determination of Serum Albumin

Although the dye-binding methods using bromcresol green and bromcresol purple, or cellulose acetate electrophoresis are widely used for the determination of albumin, there have been some problems in application to the clinical analysis of serum albumin. Therefore, more accurate determination of serum albumin is necessary for the clinical laboratory. We studied on the immunological methods (single radial immunodiffusion and immunochemical system) for the determination of serum albumin, in order to compare with the dye-binding methods. The results of the determination of purified human albumin and serum albumin in normal subjects and patients with nephrotic syndrom indicated that the immunological methods gave more reliable value of serum albumin than the dye-binding methods

FNAS/advanced protein crystal growth

A scintillation method is presented for determination of the temperature dependence of the solubility, S(T), of proteins in 50-100 micro-l volumes of solution. S(T) data for lysozyme and horse serum albumin were obtained for various combinations of pH and precipitant concentrations. The resulting kinetics and equilibrium information was used for dynamic control, that is the separation of nucleation and growth stages in protein crystallization. Individual lysozyme and horse serum albumin crystals were grown in 15-20 micro-l solution volumes contained in x-ray capillaries