Although the dye-binding methods using bromcresol green and bromcresol purple, or cellulose acetate electrophoresis are widely used for the determination of albumin, there have been some problems in application to the clinical analysis of serum albumin. Therefore, more accurate determination of serum albumin is necessary for the clinical laboratory. We studied on the immunological methods (single radial immunodiffusion and immunochemical system) for the determination of serum albumin, in order to compare with the dye-binding methods. The results of the determination of purified human albumin and serum albumin in normal subjects and patients with nephrotic syndrom indicated that the immunological methods gave more reliable value of serum albumin than the dye-binding methods
