11,930 research outputs found
Accurate detection of dysmorphic nuclei using dynamic programming and supervised classification
A vast array of pathologies is typified by the presence of nuclei with an abnormal morphology. Dysmorphic nuclear phenotypes feature dramatic size changes or foldings, but also entail much subtler deviations such as nuclear protrusions called blebs. Due to their unpredictable size, shape and intensity, dysmorphic nuclei are often not accurately detected in standard image analysis routines. To enable accurate detection of dysmorphic nuclei in confocal and widefield fluorescence microscopy images, we have developed an automated segmentation algorithm, called Blebbed Nuclei Detector (BleND), which relies on two-pass thresholding for initial nuclear contour detection, and an optimal path finding algorithm, based on dynamic programming, for refining these contours. Using a robust error metric, we show that our method matches manual segmentation in terms of precision and outperforms state-of-the-art nuclear segmentation methods. Its high performance allowed for building and integrating a robust classifier that recognizes dysmorphic nuclei with an accuracy above 95%. The combined segmentation-classification routine is bound to facilitate nucleus-based diagnostics and enable real-time recognition of dysmorphic nuclei in intelligent microscopy workflows
Graph Spectral Image Processing
Recent advent of graph signal processing (GSP) has spurred intensive studies
of signals that live naturally on irregular data kernels described by graphs
(e.g., social networks, wireless sensor networks). Though a digital image
contains pixels that reside on a regularly sampled 2D grid, if one can design
an appropriate underlying graph connecting pixels with weights that reflect the
image structure, then one can interpret the image (or image patch) as a signal
on a graph, and apply GSP tools for processing and analysis of the signal in
graph spectral domain. In this article, we overview recent graph spectral
techniques in GSP specifically for image / video processing. The topics covered
include image compression, image restoration, image filtering and image
segmentation
Computerized Analysis of Magnetic Resonance Images to Study Cerebral Anatomy in Developing Neonates
The study of cerebral anatomy in developing neonates is of great importance for
the understanding of brain development during the early period of life. This
dissertation therefore focuses on three challenges in the modelling of cerebral
anatomy in neonates during brain development. The methods that have been
developed all use Magnetic Resonance Images (MRI) as source data.
To facilitate study of vascular development in the neonatal period, a set of image
analysis algorithms are developed to automatically extract and model cerebral
vessel trees. The whole process consists of cerebral vessel tracking from
automatically placed seed points, vessel tree generation, and vasculature
registration and matching. These algorithms have been tested on clinical Time-of-
Flight (TOF) MR angiographic datasets.
To facilitate study of the neonatal cortex a complete cerebral cortex segmentation
and reconstruction pipeline has been developed. Segmentation of the neonatal
cortex is not effectively done by existing algorithms designed for the adult brain
because the contrast between grey and white matter is reversed. This causes pixels
containing tissue mixtures to be incorrectly labelled by conventional methods. The
neonatal cortical segmentation method that has been developed is based on a novel
expectation-maximization (EM) method with explicit correction for mislabelled
partial volume voxels. Based on the resulting cortical segmentation, an implicit
surface evolution technique is adopted for the reconstruction of the cortex in
neonates. The performance of the method is investigated by performing a detailed
landmark study.
To facilitate study of cortical development, a cortical surface registration algorithm
for aligning the cortical surface is developed. The method first inflates extracted
cortical surfaces and then performs a non-rigid surface registration using free-form
deformations (FFDs) to remove residual alignment. Validation experiments using
data labelled by an expert observer demonstrate that the method can capture local
changes and follow the growth of specific sulcus
A Framework for Symmetric Part Detection in Cluttered Scenes
The role of symmetry in computer vision has waxed and waned in importance
during the evolution of the field from its earliest days. At first figuring
prominently in support of bottom-up indexing, it fell out of favor as shape
gave way to appearance and recognition gave way to detection. With a strong
prior in the form of a target object, the role of the weaker priors offered by
perceptual grouping was greatly diminished. However, as the field returns to
the problem of recognition from a large database, the bottom-up recovery of the
parts that make up the objects in a cluttered scene is critical for their
recognition. The medial axis community has long exploited the ubiquitous
regularity of symmetry as a basis for the decomposition of a closed contour
into medial parts. However, today's recognition systems are faced with
cluttered scenes, and the assumption that a closed contour exists, i.e. that
figure-ground segmentation has been solved, renders much of the medial axis
community's work inapplicable. In this article, we review a computational
framework, previously reported in Lee et al. (2013), Levinshtein et al. (2009,
2013), that bridges the representation power of the medial axis and the need to
recover and group an object's parts in a cluttered scene. Our framework is
rooted in the idea that a maximally inscribed disc, the building block of a
medial axis, can be modeled as a compact superpixel in the image. We evaluate
the method on images of cluttered scenes.Comment: 10 pages, 8 figure
Disconnected Skeleton: Shape at its Absolute Scale
We present a new skeletal representation along with a matching framework to
address the deformable shape recognition problem. The disconnectedness arises
as a result of excessive regularization that we use to describe a shape at an
attainably coarse scale. Our motivation is to rely on the stable properties of
the shape instead of inaccurately measured secondary details. The new
representation does not suffer from the common instability problems of
traditional connected skeletons, and the matching process gives quite
successful results on a diverse database of 2D shapes. An important difference
of our approach from the conventional use of the skeleton is that we replace
the local coordinate frame with a global Euclidean frame supported by
additional mechanisms to handle articulations and local boundary deformations.
As a result, we can produce descriptions that are sensitive to any combination
of changes in scale, position, orientation and articulation, as well as
invariant ones.Comment: The work excluding {\S}V and {\S}VI has first appeared in 2005 ICCV:
Aslan, C., Tari, S.: An Axis-Based Representation for Recognition. In
ICCV(2005) 1339- 1346.; Aslan, C., : Disconnected Skeletons for Shape
Recognition. Masters thesis, Department of Computer Engineering, Middle East
Technical University, May 200
Deformable Prototypes for Encoding Shape Categories in Image Databases
We describe a method for shape-based image database search that uses deformable prototypes to represent categories. Rather than directly comparing a candidate shape with all shape entries in the database, shapes are compared in terms of the types of nonrigid deformations (differences) that relate them to a small subset of representative prototypes. To solve the shape correspondence and alignment problem, we employ the technique of modal matching, an information-preserving shape decomposition for matching, describing, and comparing shapes despite sensor variations and nonrigid deformations. In modal matching, shape is decomposed into an ordered basis of orthogonal principal components. We demonstrate the utility of this approach for shape comparison in 2-D image databases.Office of Naval Research (Young Investigator Award N00014-06-1-0661
Data-Driven Shape Analysis and Processing
Data-driven methods play an increasingly important role in discovering
geometric, structural, and semantic relationships between 3D shapes in
collections, and applying this analysis to support intelligent modeling,
editing, and visualization of geometric data. In contrast to traditional
approaches, a key feature of data-driven approaches is that they aggregate
information from a collection of shapes to improve the analysis and processing
of individual shapes. In addition, they are able to learn models that reason
about properties and relationships of shapes without relying on hard-coded
rules or explicitly programmed instructions. We provide an overview of the main
concepts and components of these techniques, and discuss their application to
shape classification, segmentation, matching, reconstruction, modeling and
exploration, as well as scene analysis and synthesis, through reviewing the
literature and relating the existing works with both qualitative and numerical
comparisons. We conclude our report with ideas that can inspire future research
in data-driven shape analysis and processing.Comment: 10 pages, 19 figure
Statistical Model of Shape Moments with Active Contour Evolution for Shape Detection and Segmentation
This paper describes a novel method for shape representation and robust image segmentation. The proposed method combines two well known methodologies, namely, statistical shape models and active contours implemented in level set framework. The shape detection is achieved by maximizing a posterior function that consists of a prior shape probability model and image likelihood function conditioned on shapes. The statistical shape model is built as a result of a learning process based on nonparametric probability estimation in a PCA reduced feature space formed by the Legendre moments of training silhouette images. A greedy strategy is applied to optimize the proposed cost function by iteratively evolving an implicit active contour in the image space and subsequent constrained optimization of the evolved shape in the reduced shape feature space. Experimental results presented in the paper demonstrate that the proposed method, contrary to many other active contour segmentation methods, is highly resilient to severe random and structural noise that could be present in the data
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