Numerical and experimental investigations of self-piercing riveting

Self-pierce riveting (SPR) is a new high-speed mechanical fastening technique which is suitable for point joining dissimilar sheet materials, as well as coated and pre-painted sheet materials. With increasing application of SPR in different industrial fields, the demand for a better understanding of the knowledge of static and dynamic characteristics of the SPR joints is required. In this paper, the SPR process has been numerically simulated using the commercial finite element (FE) software LS-Dyna. For validating the numerical simulation of the SPR process, experimental tests on specimens made of aluminium alloy have been carried out. The online window monitoring technique was introdu introdu ced in the tests for evaluating the quality of SPR joints. Good agreements between the simulations and the tests have been found, both with respect to the force-travel (time) curves as well as the deformed shape on the cross-section of SPR joint. Monotonic tensile tests were carried out to measure the ultimate tensile strengths for SPR joints with different material combinations. Deformation and failure of the SPR joints under monotonic tensile loading were studied. The normal hypothesis tests were performed to examine the rationality of the test data. This work was also aimed at evaluating experimentally and comparing the strength and energy absorption of SPR joints and SPR-bonded hybrid joints

MicroRNA detection on microsensor arrays by SPR imaging measurements with enzymatic signal enhancement.

We investigated sequence-specific and simultaneous microRNA (miRNA) detections by surface plasmon resonance (SPR) imaging measurements on SPR chips possessing an Au spot array modified with probe DNAs based on a miRNA-detection-selective SPR signal amplification method. MiRNAs were detected with the detection limit of the attomole level by SPR imaging measurements for different miRNA concentrations on a single chip. SPR signals were enhanced based on a combination process of sequence-specific hybridization of the miRNA to the probe DNAs, extension reaction of polyadenine (poly(A)) tails by poly(A) polymerase, binding of a ternary complex of T30-biotin/horseradish peroxidase (HRP)-biotin/streptavidin to the poly(A) tails, and the oxidation reaction of tetramethylbenzidine (TMB) on the HRP by providing a blue precipitate on the surface. This process sequence-specifically and dramatically amplified the SPR signals. This is a simple, cost-effective, and feasible signal amplification method based on the organic compound TMB instead of metal nanoparticles

Simultaneous Surface Plasmon Resonance and X-ray Absorption Spectroscopy

We present here an experimental set-up to perform simultaneously measurements of surface plasmon resonance (SPR) and X-ray absorption spectroscopy (XAS) in a synchrotron beamline. The system allows measuring in situ and in real time the effect of X-ray irradiation on the SPR curves to explore the interaction of X-rays with matter. It is also possible to record XAS spectra while exciting SPR in order to detect the changes in the electronic configuration of thin films induced by the excitation of surface plasmons. Combined experiments recording simultaneously SPR and XAS curves while scanning different parameters can be carried out. The relative variations in the SPR and XAS spectra that can be detected with this set-up ranges from 10-3 to 10-5, depending on the particular experiment

A FDTD model for the post-reception synthetic focusing surface penetrating radar with mine detecting applications

Surface penetrating radar (SPR) is extensively used in military, civil, geophysical and archaeological applications. There has been an increasing emphasis on the use of SPR in the identification of buried unexploded ordnance, such as anti-tank mines, antipersonnel mines etc., which can be metallic or plastic in nature. Mine detection methods can be classified as destructive or non-destructive. Non-destructive mine detection methods employ metal detectors, magnetometers, infrared sensors and SPR. However, neither the metal detector nor the magnetometer can differentiate a mine from metallic debris and the infrared sensor merely notes a difference in thermal conductivity. In most battle fields the soil is contaminated by large quantity of shrapnel, metal scraps and cartridge cases which will give a high false alarm rate in the identification process. In this scenario the SPR is a promising technique for identification of both metallic and plastic mines. The use of finite difference time domain (FDTD) model to analyse the SPR, which makes use of post-reception synthetic focusing (PRSF) techniques, is presented

Ligand-receptor binding kinetics in surface plasmon resonance cells: A Monte Carlo analysis

Surface plasmon resonance (SPR) chips are widely used to measure association and dissociation rates for the binding kinetics between two species of chemicals, e.g., cell receptors and ligands. It is commonly assumed that ligands are spatially well mixed in the SPR region, and hence a mean-field rate equation description is appropriate. This approximation however ignores the spatial fluctuations as well as temporal correlations induced by multiple local rebinding events, which become prominent for slow diffusion rates and high binding affinities. We report detailed Monte Carlo simulations of ligand binding kinetics in an SPR cell subject to laminar flow. We extract the binding and dissociation rates by means of the techniques frequently employed in experimental analysis that are motivated by the mean-field approximation. We find major discrepancies in a wide parameter regime between the thus extracted rates and the known input simulation values. These results underscore the crucial quantitative importance of spatio-temporal correlations in binary reaction kinetics in SPR cell geometries, and demonstrate the failure of a mean-field analysis of SPR cells in the regime of high Damk\"ohler number Da > 0.1, where the spatio-temporal correlations due to diffusive transport and ligand-receptor rebinding events dominate the dynamics of SPR systems.Comment: 21 pages, 9 figure

Self-propelled rods exhibit a novel phase-separated state characterized by the presence of active stresses and the ejection of polar clusters

We study collections of self-propelled rods (SPR) moving in two dimensions for packing fractions less than or equal to 0.3. We find that in the thermodynamical limit the SPR undergo a phase transition between a disordered gas and a novel phase-separated system state. Interestingly, (global) orientational order patterns -- contrary to what has been suggested -- vanish in this limit. In the found novel state, the SPR self-organize into a highly dynamical, high-density, compact region - which we call aggregate - which is surrounded by a disordered gas. Active stresses build inside aggregates as result of the combined effect of local orientational order and active forces. This leads to the most distinctive feature of these aggregates: constant ejection of polar clusters of SPR. This novel phase-separated state represents a novel state of matter characterized by large fluctuations in volume and shape, related to mass ejection, and exhibits positional as well as orientational local order. SPR systems display new physics unseen in other active matter systems due to the coupling between density, active stresses, and orientational order (such coupling cannot be reduced simply to a coupling between speed and density).Comment: to appear in PR

Infra-Red Surface-Plasmon-Resonance technique for biological studies

We report on a Surface-Plasmon-Resonance (SPR) technique based on Fourier -Transform - Infra - Red (FTIR) spectrometer. In contrast to the conventional surface plasmon technique, operating at a fixed wavelength and a variable angle of incidence, our setup allows the wavelength and the angle of incidence to be varied simultaneously. We explored the potential of the SPR technique in the infrared for biological studies involving aqueous solutions. Using computer simulations, we found the optimal combination of parameters (incident angle, wavelength) for performing this task. Our experiments with physiologically important glucose concentrations in water and in human plasma verified our computer simulations. Importantly, we demonstrated that the sensitivity of the SPR technique in the infrared range is not lower and in fact is even higher than that for visible light. We emphasize the advantages of infra red SPR for studying glucose and other biological molecules in living cells.Comment: 8 pages,8 figure

Modelling of self-piercing riveting with ale, cel and sph based on abaqus/explicit

SPR (Self-piercing riveting) is a cold forming process that is used to fasten together two or more sheets of different materials mechanically with a rivet. Also SPR emulates the results and quality of spot welding without many of the risks, such as toxic fumes, sparks and noise. Thus circumstanced, this technique is widely used on the various filed especially within automobile industry. SPR, in particular, is excellent for lightweight manufacturing and for precise working while dramatically reducing cost and production time. The process deformation depends on the sheet size, shape of die, material flow, stiffness, etc. Also material deformation in both of rivet and workpiece sheets is tremendous large, for instance thinning, necking, shear and penetration. Therefore it is very hard to analyze this forming process with FEM which uses normal stress element formulation due to the collapse. On the other hand, Abaqus/Explicit has superb analysis methods, for example ALE, CEL and SPH[1]. This paper investigates several Abaqus/Explicit modeling techniques for simulating and optimizing SPR process. In addition, the effectiveness of these analysis methods was discussed and compared for evaluating SPR process forming in order to achieve an optimal die, material properties and suitability of deformations