Extracting 3D parametric curves from 2D images of Helical objects

Helical objects occur in medicine, biology, cosmetics, nanotechnology, and engineering. Extracting a 3D parametric curve from a 2D image of a helical object has many practical applications, in particular being able to extract metrics such as tortuosity, frequency, and pitch. We present a method that is able to straighten the image object and derive a robust 3D helical curve from peaks in the object boundary. The algorithm has a small number of stable parameters that require little tuning, and the curve is validated against both synthetic and real-world data. The results show that the extracted 3D curve comes within close Hausdorff distance to the ground truth, and has near identical tortuosity for helical objects with a circular profile. Parameter insensitivity and robustness against high levels of image noise are demonstrated thoroughly and quantitatively

Quantification of the plant endoplasmic reticulum

One of the challenges of quantitative approaches to biological sciences is the lack of understanding of the interplay between form and function. Each cell is full of complex-shaped objects, which moreover change their form over time. To address this issue, we exploit recent advances in confocal microscopy, by using data collected from a series of optical sections taken at short regular intervals along the optical axis to reconstruct the Endoplasmic Reticulum (ER) in 3D, obtain its skeleton, then associate to each of its edges key geometric and dynamic characteristics obtained from the original filled in ER specimen. These properties include the total length, surface area, and volume of the ER specimen, as well as the length surface area, and volume of each of its branches. In a view to benefit from the well established graph theory algorithms, we abstract the obtained skeleton by a mathematical entity that is a graph. We achieve this by replacing the inner points in each edge in the skeleton by the line segment connecting its end points. We then attach to this graph the ER geometric properties as weights, allowing therefore a more precise quantitative characterisation, by thinning the filled in ER to its essential features. The graph plays a major role in this study and is the final and most abstract quantification of the ER. One of its advantages is that it serves as a geometric invariant, both in static and dynamic samples. Moreover, graph theoretic features, such as the number of vertices and their degrees, and the number of edges and their lengths are robust against different kinds of small perturbations. We propose a methodology to associate parameters such as surface areas and volumes to its individual edges and monitor their variations with time. One of the main contributions of this thesis is the use of the skeleton of the ER to analyse the trajectories of moving junctions using confocal digital videos. We report that the ER could be modeled by a network of connected cylinders (0.87μm±0.36 in diameter) with a majority of 3-way junctions. The average length, surface area and volume of an ER branch are found to be 2.78±2.04μm, 7.53±5.59μm2 and 1.81±1.86μm3 respectively. Using the analysis of variance technique we found that there are no significant differences in four different locations across the cell at 0.05 significance level. The apparent movement of the junctions in the plant ER consists of different types, namely: (a) the extension and shrinkage of tubules, and (b) the closing and opening of loops. The average velocity of a junction is found to be 0.25μm/sec±0.23 and lies in the range 0 to 1.7μm/sec which matches the reported actin filament range

Skeletonization methods for image and volume inpainting

Image and shape restoration techniques are increasingly important in computer graphics. Many types of restoration techniques have been proposed in the 2D image-processing and according to our knowledge only one to volumetric data. Well-known examples of such techniques include digital inpainting, denoising, and morphological gap filling. However efficient and effective, such methods have several limitations with respect to the shape, size, distribution, and nature of the defects they can find and eliminate. We start by studying the use of 2D skeletons for the restoration of two-dimensional images. To this end, we show that skeletons are useful and efficient for volumetric data reconstruction. To explore our hypothesis in the 3D case, we first overview the existing state-of-the-art in 3D skeletonization methods, and conclude that no such method provides us with the features required by efficient and effective practical usage. We next propose a novel method for 3D skeletonization, and show how it complies with our desired quality requirements, which makes it thereby suitable for volumetric data reconstruction context. The joint results of our study show that skeletons are indeed effective tools to design a variety of shape restoration methods. Separately, our results show that suitable algorithms and implementations can be conceived to yield high end-to-end performance and quality of skeleton-based restoration methods. Finally, our practical applications can generate competitive results when compared to application areas such as digital hair removal and wire artifact removal

Shape analysis and description based on the isometric invariances of topological skeletonization

ilustracionesIn this dissertation, we explore the problem of how to describe the shape of an object in 2D and 3D with a set of features that are invariant to isometric transformations. We focus to based our approach on the well-known Medial Axis Transform and its topological properties. We aim to study two problems. The first is how to find a shape representation of a segmented object that exhibits rotation, translation, and reflection invariance. The second problem is how to build a machine learning pipeline that uses the isometric invariance of the shape representation to do both classification and retrieval. Our proposed solution demonstrates competitive results compared to state-of-the-art approaches. We based our shape representation on the medial axis transform (MAT), sometimes called the topological skeleton. Accepted and well-studied properties of the medial axis include: homotopy preservation, rotation invariance, mediality, one pixel thickness, and the ability to fully reconstruct the object. These properties make the MAT a suitable input to create shape features; however, several problems arise because not all skeletonization methods satisfy all the above-mentioned properties at the same time. In general, skeletons based on thinning approaches preserve topology but are noise sensitive and do not allow a proper reconstruction. They are also not invariant to rotations. Voronoi skeletons also preserve topology and are rotation invariant, but do not have information about the thickness of the object, making reconstruction impossible. The Voronoi skeleton is an approximation of the real skeleton. The denser the sampling of the boundary, the better the approximation; however, a denser sampling makes the Voronoi diagram more computationally expensive. In contrast, distance transform methods allow the reconstruction of the original object by providing the distance from every pixel in the skeleton to the boundary. Moreover, they exhibit an acceptable degree of the properties listed above, but noise sensitivity remains an issue. Therefore, we selected distance transform medial axis methods as our skeletonization strategy, and focused on creating a new noise-free approach to solve the contour noise problem. To effectively classify an object, or perform any other task with features based on its shape, the descriptor needs to be a normalized, compact form: $\Phi$ should map every shape $\Omega$ to the same vector space $\mathrm{R}^{n}$. This is not possible with skeletonization methods because the skeletons of different objects have different numbers of branches and different numbers of points, even when they belong to the same category. Consequently, we developed a strategy to extract features from the skeleton through the map $\Phi$, which we used as an input to a machine learning approach. After developing our method for robust skeletonization, the next step is to use such skeleton into the machine learning pipeline to classify object into previously defined categories. We developed a set of skeletal features that were used as input data to the machine learning architectures. We ran experiments on MPEG7 and ModelNet40 dataset to test our approach in both 2D and 3D. Our experiments show results comparable with the state-of-the-art in shape classification and retrieval. Our experiments also show that our pipeline and our skeletal features exhibit some degree of invariance to isometric transformations. In this study, we sought to design an isometric invariant shape descriptor through robust skeletonization enforced by a feature extraction pipeline that exploits such invariance through a machine learning methodology. We conducted a set of classification and retrieval experiments over well-known benchmarks to validate our proposed method. (Tomado de la fuente)En esta disertación se explora el problema de cómo describir la forma de un objeto en 2D y 3D con un conjunto de características que sean invariantes a transformaciones isométricas. La metodología propuesta en este documento se enfoca en la Transformada del Eje Medio (Medial Axis Transform) y sus propiedades topológicas. Nuestro objetivo es estudiar dos problemas. El primero es encontrar una representación matemática de la forma de un objeto que exhiba invarianza a las operaciones de rotación, translación y reflexión. El segundo problema es como construir un modelo de machine learning que use esas invarianzas para las tareas de clasificación y consulta de objetos a través de su forma. El método propuesto en esta tesis muestra resultados competitivos en comparación con otros métodos del estado del arte. En este trabajo basamos nuestra representación de forma en la transformada del eje medio, a veces llamada esqueleto topológico. Algunas propiedades conocidas y bien estudiadas de la transformada del eje medio son: conservación de la homotopía, invarianza a la rotación, su grosor consiste en un solo pixel (1D), y la habilidad para reconstruir el objeto original a través de ella. Estas propiedades hacen de la transformada del eje medio un punto de partida adecuado para crear características de forma. Sin embargo, en este punto surgen varios problemas dado que no todos los métodos de esqueletización satisfacen, al mismo tiempo, todas las propiedades mencionadas anteriormente. En general, los esqueletos basados en enfoques de erosión morfológica conservan la topología del objeto, pero son sensibles al ruido y no permiten una reconstrucción adecuada. Además, no son invariantes a las rotaciones. Otro método de esqueletización son los esqueletos de Voronoi. Los esqueletos de Voronoi también conservan la topología y son invariantes a la rotación, pero no tienen información sobre el grosor del objeto, lo que hace imposible su reconstrucción. Cuanto más denso sea el muestreo del contorno del objeto, mejor será la aproximación. Sin embargo, un muestreo más denso hace que el diagrama de Voronoi sea más costoso computacionalmente. Por el contrario, los métodos basados en la transformada de la distancia permiten la reconstrucción del objeto original, ya que proporcionan la distancia desde cada píxel del esqueleto hasta su punto más cercano en el contorno. Además, exhiben un grado aceptable de las propiedades enumeradas anteriormente, aunque la sensibilidad al ruido sigue siendo un problema. Por lo tanto, en este documento seleccionamos los métodos basados en la transformada de la distancia como nuestra estrategia de esqueletización, y nos enfocamos en crear un nuevo enfoque que resuelva el problema del ruido en el contorno. Para clasificar eficazmente un objeto o realizar cualquier otra tarea con características basadas en su forma, el descriptor debe ser compacto y estar normalizado: $\Phi$ debe relacionar cada forma $\Omega$ al mismo espacio vectorial $\mathrm{R}^{n}$. Esto no es posible con los métodos de esqueletización en el estado del arte, porque los esqueletos de diferentes objetos tienen diferentes números de ramas y diferentes números de puntos incluso cuando pertenecen a la misma categoría. Consecuentemente, en nuestra propuesta desarrollamos una estrategia para extraer características del esqueleto a través de la función $\Phi$, que usamos como entrada para un enfoque de aprendizaje automático. % TODO completar con resultados. Después de desarrollar nuestro método de esqueletización robusta, el siguiente paso es usar dicho esqueleto en un modelo de aprendizaje de máquina para clasificar el objeto en categorías previamente definidas. Para ello se desarrolló un conjunto de características basadas en el eje medio que se utilizaron como datos de entrada para la arquitectura de aprendizaje automático. Realizamos experimentos en los conjuntos de datos: MPEG7 y ModelNet40 para probar nuestro enfoque tanto en 2D como en 3D. Nuestros experimentos muestran resultados comparables con el estado del arte en clasificación y consulta de formas (retrieval). Nuestros experimentos también muestran que el modelo desarrollado junto con nuestras características basadas en el eje medio son invariantes a las transformaciones isométricas. (Tomado de la fuente)Beca para Doctorados Nacionales de Colciencias, convocatoria 725 de 2015DoctoradoDoctor en IngenieríaVisión por computadora y aprendizaje automátic

Extracting 3D parametric curves from 2D images of helical objects

Helical objects occur in medicine, biology, cosmetics, nanotechnology, and engineering. Extracting a 3D parametric curve from a 2D image of a helical object has many practical applications, in particular being able to extract metrics such as tortuosity, frequency, and pitch. We present a method that is able to straighten the image object and derive a robust 3D helical curve from peaks in the object boundary. The algorithm has a small number of stable parameters that require little tuning, and the curve is validated against both synthetic and real-world data. The results show that the extracted 3D curve comes within close Hausdorff distance to the ground truth, and has near identical tortuosity for helical objects with a circular profile. Parameter insensitivity and robustness against high levels of image noise are demonstrated thoroughly and quantitatively