Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition

The treatment and disposal of domestic sIudge is an expensive and environmentally sensitive problem. It is also a growing problem since sludge production will continue to increase as new wastewzter treatment plants are built due to population increase. The large volume of domestic sIudge produced had made it difficult for many countries including Malaysia to assure complete treatment of the sludge before discharging to the receiving environment. Domestic sludge contains diverse range of pollutants such as pathogen, inorganic and organic compounds. These pollutants are toxic, mutagenic or carcinogenic and may threaten human health. Iiilproper disposal and handling of sludge may pose serious impact to the environment especially on soil and water cycles. Previous studies on Malaysian domestic sludge only reported on bulk parameters and heavy metals. Thus, no study reported on organic micro pollutants, namely, polycylic aromatic hydrocarbons (PAHs). Their recalcitrance and persistence make them problematic environmental contaminants. Microbial degradation is considered to be the primary mechanism of PAHs removal from the environment. Much has been reported on biodegradation of PAHs in several countries but there is a lack of information quantitative on this subject in Malaysia. This study is carried out to understand the nature of domestic sludge and to provide a better understanding on the biodegradation processes of PAHs. The methodology of this study comprised field activities, laboratory work and mathematical modelling. Field activities involved sampling of domestic sludge from Kolej Mawar, Universiti Teknologi MARA, Shah Alam, Selangor. Laboratory activities include seven phases of experimental works. First phase is characterization study of domestic sludge based on bulk parameters, heavy metals and PAHs. Second phase is enrichment and purification of bacteria isolated from domestic sludge using single PAHs and mixed PAHs as growth substrate. This was followed by identification of bacteria using BIOLOG system. The fourth phase focussed on turbidity test to monitor growth rate of the isolated bacteria. Preliminary degradation study involves optimization of the process at different substrate concentration, bacteria concentration, pH and temperature. The optimum conditions established from optimization study were used in degradation study. In biodegradation study, two experimental conditions were performed. These conditions include using bacteria isolated from single PAHs as substrate and bacteria isolated from mixed PAHs. Protein and pH tests were done during degradation study. Final activity is mathematical modelling of the biodegradation process. In general results on bulk parameters are comparable to previous studies. Zinc was the main compound with a mean concentration of 11 96.4 mglkg. PAHs were also detected in all of the samples, with total concentration between 0.72 to 5.36 mglkg dry weight for six PAHs. In the examined samples, phenanthrene was the main compound with a mean concentration of 1.0567 mglkg. The results fiom purification studies of bacteria strains sucessfull isolated 13 bacteria strains fiom single PAH substrate while three bacteria were isolated from the mixed PAHs substrate. Based on bacteria growth rates, only six strains grown on single PAHs and three strains grown on mixed PAHs were used for further studies. Results from the optimization study of biodegradation indicated that maximum rate of PAHs removal occurred at 100 mg~-' of PAHs, 10% bacteria concentration, pH 7.0 and 30°C. The results showed that bacteria grown on lower ring of PAHs are not able to grow on higher ring of PAHs. As for example Micrococcus diversus grown on napthalene as sole carbon source was unable to degrade other PAHs like acenapthylene, acenapthene, fluorene, phenanthrene and antlracene. In the case of bacteria isolated from mixed PAHs, the results showed that most of the napthalene was degraded by isolated strains with the highest average degradation rate followed by acenapthylene, acenapthene, fluorene, phenanthrene and anthracene. 377$3(53867$.$$1�781.8�781�$0,1$+ D4ff + c\,cpda~ition trends were observed in the study could be attributed to the different subsr , i,lo\~ir 'Led during isolation process. Interaction through cometabolism and synergistic ocolq bacteria strains isolated from single substrate. Thus, only synergistic interaction was oL, :a 77ed for bacteria isolated from mixed substrate. Corynebacterium urolyticum re\e;;ed I,, be the best strain in degrading PAHs. The experimental results have led to a model conccl~t desclibing I'AHs degradation

Research on Quality of Service Based Routing Protocols for Mobile Ad Hoc Networks

Quality of service (QoS) based routing protocols play a significant role in MANETs to maintain proper flow of data with efficient power consumption and without data loss. However, several network resource based technical challenges or issues are encountered in the design and implementation of QoS routing protocols that perform their routing function by considering the shortest route or the lowest cost. Furthermore, a secondary route is not reserved and alternative routes are not searched unless the established route is broken. The current structures of the state-of-the-art protocols for MANETs are not appropriate for today's high bandwidth and mobility requirements. Therefore, research on new routing protocols is needed, considering energy level, coverage, location, speed, movement, and link stability instead of only shortest path and lowest cost. This paper summarizes the main characteristics of QoS-based routing protocols to facilitate researchers to design and select QoS-based routing protocols. In this study, a wide range of protocols with their characteristics were classified according to QoS routing strategy, routing information update mechanism, interaction between network and MAC layer, QoS constraints, QoS guarantee type and number of discovered routes. In addition, the protocols were compared in terms of properties, design features, challenges and QoS metrics

Flexible cross layer design for improved quality of service in MANETs

This thesis was submitted for the degree of Doctor of Philosophy and was awarded by Brunel UniversityMobile Ad hoc Networks (MANETs) are becoming increasingly important because of their unique characteristics of connectivity. Several delay sensitive applications are starting to appear in these kinds of networks. Therefore, an issue in concern is to guarantee Quality of Service (QoS) in such constantly changing communication environment. The classical QoS aware solutions that have been used till now in the wired and infrastructure wireless networks are unable to achieve the necessary performance in the MANETs. The specialized protocols designed for multihop ad hoc networks offer basic connectivity with limited delay awareness and the mobility factor in the MANETs makes them even more unsuitable for use. Several protocols and solutions have been emerging in almost every layer in the protocol stack. The majority of the research efforts agree on the fact that in such dynamic environment in order to optimize the performance of the protocols, there is the need for additional information about the status of the network to be available. Hence, many cross layer design approaches appeared in the scene. Cross layer design has major advantages and the necessity to utilize such a design is definite. However, cross layer design conceals risks like architecture instability and design inflexibility. The aggressive use of cross layer design results in excessive increase of the cost of deployment and complicates both maintenance and upgrade of the network. The use of autonomous protocols like bio-inspired mechanisms and algorithms that are resilient on cross layer information unavailability, are able to reduce the dependence on cross layer design. In addition, properties like the prediction of the dynamic conditions and the adaptation to them are quite important characteristics. The design of a routing decision algorithm based on Bayesian Inference for the prediction of the path quality is proposed here. The accurate prediction capabilities and the efficient use of the plethora of cross layer information are presented. Furthermore, an adaptive mechanism based on the Genetic Algorithm (GA) is used to control the flow of the data in the transport layer. The aforementioned flow control mechanism inherits GA’s optimization capabilities without the need of knowing any details about the network conditions, thus, reducing the cross layer information dependence. Finally, is illustrated how Bayesian Inference can be used to suggest configuration parameter values to the other protocols in different layers in order to improve their performance.National Foundation of Scholarships of Greece(I.K.Y.

Rough set based QoS enabled multipath source routing in MANET

The single constrained Quality of Service (QoS) routing in Mobile Ad-hoc NETwork (MANET) is disastrous in consideration of MANET characteristics, inference, collision and link failure as it maintains a single path. The QoS enabled routing yields better packet delivery and maintains consistency among nodes in the network by incorporating multi-constrained and multipath routing. The Dynamic Source Routing (DSR) is best suited source routing algorithm to maintain multipath information at the source node, but performance degrades with larger number of mobile nodes. Multi-layer mechanism should be incorporated to maintain QoS metric information spreads across multiple layers of TCP/IP protocol stack. The proposed multipath QoS enabled source routing provides balanced routing by making use of all these features. The imprecise decision making strategy called Rough Set Theory (RST) is used at destination node for decision making. The Route REQuest (RREQ) messages coming from different routes are filtered by considering the QoS metrics of each and every route by making use of RST. The Route REPly (RREP) messages are generated and delivered to the source node for filtered RREQ messages. The proposed routing algorithm will reduce load on the network by reducing number of control messages exchanged for route establishment. This will evenly distribute load among all the nodes and it also avoid the scenarios like few nodes starved for resources. Finally, multipath routing always provides alternate routing option in case of route failure

Mobile Ad-Hoc Networks

Being infrastructure-less and without central administration control, wireless ad-hoc networking is playing a more and more important role in extending the coverage of traditional wireless infrastructure (cellular networks, wireless LAN, etc). This book includes state-of-the-art techniques and solutions for wireless ad-hoc networks. It focuses on the following topics in ad-hoc networks: quality-of-service and video communication, routing protocol and cross-layer design. A few interesting problems about security and delay-tolerant networks are also discussed. This book is targeted to provide network engineers and researchers with design guidelines for large scale wireless ad hoc networks