From roses to bullets: the rise and decline of post-Soviet colour revolutions

The chapter explores the reasons for the colour revolutions’ successes and failures in the post-Soviet space. The article starts with an overview on the colour movement from the first stirrings to the present day. We then propose criteria that will be applied to our analysis, constructed on five variables. The factual analysis of individual countries that follows is built around these five variables

Optimizing the identification of risk-relevant mutations by multigene panel testing in selected hereditary breast/ovarian cancer families

The introduction of multigene panel testing for hereditary breast/ovarian cancer screening has greatly improved efficiency, speed, and costs. However, its clinical utility is still debated, mostly due to the lack of conclusive evidences on the impact of newly discovered genetic variants on cancer risk and lack of evidence-based guidelines for the clinical management of their carriers. In this pilot study, we aimed to test whether a systematic and multiparametric characterization of newly discovered mutations could enhance the clinical utility of multigene panel sequencing. Out of a pool of 367 breast/ovarian cancer families Sanger-sequenced for BRCA1 and BRCA2 gene mutations, we selected a cohort of 20 BRCA1/2-negative families to be subjected to the BROCA-Cancer Risk Panel massive parallel sequencing. As a strategy for the systematic characterization of newly discovered genetic variants, we collected blood and cancer tissue samples and established lymphoblastoid cell lines from all available individuals in these families, to perform segregation analysis, loss-of-heterozygosity and further molecular studies. We identified loss-of-function mutations in 6 out 20 high-risk families, 5 of which occurred on BRCA1, CHEK2 and ATM and are esteemed to be risk-relevant. In contrast, a novel RAD50 truncating mutation is most likely unrelated to breast cancer. Our data suggest that integrating multigene panel testing with a pre-organized, multiparametric characterization of newly discovered genetic variants improves the identification of risk-relevant alleles impacting on the clinical management of their carriers

Comparison of some chemical and non-chemical treatments to disinfect a recirculating nutrient solution

Closed hydroponic growing systems have a better water use efficiency (WUE) and a lower use of fertilizers, but a larger risk of spreading soil-borne pathogens all over the crop compared to open systems. In climates or regions where availability of water is limited closed systems should be preferred above open systems but the risk of spreading soil-borne pathogens should be minimized. Disinfection of the nutrient solution is a valuable method, but it often demands high investments. A desk study was made to compare the performance of some chemical and non-chemical treatments. For larger companies (>2 ha) heat treatment and UV radiation are still the best options. For smaller companies

Disease management in soilless culture systems

EU legislation, laid down in the Water Framework Directive, demands to minimize emissions of nitrogen, phosphate and crop protection products to achieve an excellent chemical and ecological quality in 2015. The aim is to force growers to a better water and disease management. Supply water of excellent chemical quality will have to be recirculated as long as possible, for which adequate disinfection equipment have to be used. Several sources of water are used as supply water. Rainwater is chemically best, followed by reverse osmosis water. However, the latter is rather expensive. Tap water and surface water often have a too high salinity, while well water may vary dramatically from place to place. Rainwater and surface water are potential risk factors for importing soil-borne pathogens. Disinfection of the recirculating nutrient solution can be done adequately by heat treatment and UV radiation. Membrane filtration performs well, but is mostly too costly. Chemical treatments as sodium hypochlorite, chlorine dioxide and copper silver ionization may partly solve the pathogen problem, but introduce a potential accumulation of other elements in closed systems. Hydrogen peroxide, chlorine dioxide and sodium hypochlorite perform better to clean pipe work instead of soil-borne pathogens

Endogenous TRPV1 stimulation leads to the activation of the inositol phospholipid pathway necessary for sustained Ca2+ oscillations

Sensory neuron subpopulations as well as breast and prostate cancer cells express functional transient receptor potential vanilloid type 1 (TRPV1) ion channels; however little is known how TRPV1 activation leads to biological responses. Agonist-induced activation of TRPV1 resulted in specific spatiotemporal patterns of cytoplasmic Ca²⁺ signals in breast and prostate cancer-derived cells. Capsaicin (CAPS; 50 μM) evoked intracellular Ca²⁺ oscillations and/or intercellular Ca²⁺ waves in all cell lines. As evidenced in prostate cancer Du 145 cells, oscillations were largely dependent on the expression of functional TRPV1 channels in the plasma membrane, phospholipase C activation and on the presence of extracellular Ca²⁺ ions. Concomitant oscillations of the mitochondrial matrix Ca²⁺ concentration resulted in mitochondria energization evidenced by increased ATP production. CAPS-induced Ca²⁺ oscillations also occurred in a subset of sensory neurons, yet already at lower CAPS concentrations (1 μM). Stimulation of ectopically expressed TRPV1 channels in CAPS-insensitive NIH- 3T3 cells didn't provoke CAPS-triggered Ca²⁺ oscillations; rather it resulted in low- magnitude, long-lasting elevations of the cytosolic Ca²⁺ concentration. This indicates that sole TRPV1 activation is not sufficient to generate Ca²⁺ oscillations. Instead the initial TRPV1-mediated signal leads to the activation of the inositol phospholipid pathway. This in turn suffices to generate a biologically relevant frequency-modulated Ca²⁺ signal

Around the table: Are multiple-touch surfaces better than single-touch for children's collaborative interactions?

This paper presents a classroom study that investigated the potential of using touch tabletop technology to support children's collaborative learning interactions. Children aged 7-10 worked in groups of three on a collaborative planning task in which they designed a seating plan for their classroom. In the single-touch condition, the tabletop surface allowed only one child to interact with the digital content at a time. In the multiple-touch condition, the children could interact with the digital content simultaneously. Results showed that touch condition did not affect the frequency or equity of interactions, but did influence the nature of children's discussion. In the multiple-touch condition, children talked more about the task; in the single-touch condition, they talked more about turn taking. We also report age and gender differences

Analysis and new constructions of generalized barycentric coordinates in 2D

Different coordinate systems allow to uniquely determine the position of a geometric element in space. In this dissertation, we consider a coordinate system that lets us determine the position of a two-dimensional point in the plane with respect to an arbitrary simple polygon. Coordinates of this system are called generalized barycentric coordinates in 2D and are widely used in computer graphics and computational mechanics. There exist many coordinate functions that satisfy all the basic properties of barycentric coordinates, but they differ by a number of other properties. We start by providing an extensive comparison of all existing coordinate functions and pointing out which important properties of generalized barycentric coordinates are not satisfied by these functions. This comparison shows that not all of existing coordinates have fully investigated properties, and we complete such a theoretical analysis for a particular one-parameter family of generalized barycentric coordinates for strictly convex polygons. We also perform numerical analysis of this family and show how to avoid computational instabilities near the polygon’s boundary when computing these coordinates in practice. We conclude this analysis by implementing some members of this family in the Computational Geometry Algorithm Library. In the second half of this dissertation, we present a few novel constructions of non-negative and smooth generalized barycentric coordinates defined over any simple polygon. In this context, we show that new coordinates with improved properties can be obtained by taking convex combinations of already existing coordinate functions and we give two examples of how to use such convex combinations for polygons without and with interior points. These new constructions have many attractive properties and perform better than other coordinates in interpolation and image deformation applications