A Rapid and Ultra-sensitive Biosensing Platform based on Tunable Dielectrophoresis for Robust POC Applications

With the ongoing pandemic, there have been increasing concerns recently regarding major public health issues such as abuse of organophosphorus compounds, pathogenic bacterial infections, and biosecurity in agricultural production. Biosensors have long been considered a kernel technology for next-generation diagnostic solutions to improve food safety and public health. Significant amounts of effort have been devoted to inventing novel sensing mechanisms, modifying their designs, improving their performance, and extending their application scopes. However, the reliability and selectivity of most biosensors still have much to be desired, which holds back the development and commercialization of biosensors, especially for on-site and point-of-care (POC) usages. Herein, we introduce an innovative two-phase sensing strategy based on tunable AC electrokinetics and capacitive sensing. By dividing the detection process into a sensitivity-priority step and a selectivity-priority step, the specificity and sensitivity of a biosensor can be significantly improved. A capacitive POC aptasensor is fabricated for the implementation of the 2-phase detection and a quasi-single-cell level detection of limit together with an excellent selectivity is achieved simultaneously. The sensor is capable of handling real-world clinic samples without sophisticated pretreatment. Just after a simple one-step dilution, the developed sensor can detect bacteria no less than 2~3 bacteria/10 µL in raw milk samples within 100 s. Alongside whole bacteria detection, the biosensor can also detect endotoxin, the lipopolysaccharide, in bovine serum samples, with a limit of detection of 10 pg/mL. The biosensor is low-cost and easy to use. This work not only demonstrates a biosensor with significant advantages in sensitivity, selectivity and assay time but also opens up a new horizon for further research of all affinity-based biosensors

Enhanced Piezoelectric Performance of Printed PZT Films on Low Temperature Substrates

Since piezoelectric effect was discovered in 1880, it has been widely used in micro-actuators, sensors, and energy harvesters. Lead Zirconate Titanate (PZT) is a commonly used piezoelectric material due to the high piezoelectric response. The basic PZT film fabrication process includes deposition, sintering, and poling. However, due to the high sintering temperature (\u3e 800 °C) of PZT, only high melting point material can be served as the substrate. Otherwise, complex film transfer approach is needed to achieve flexible and foldable PZT devices. The exploration is accordingly necessary to realize direct fabrication of PZT films on low melting point substrates without affecting the piezoelectric performance. In order to lower the PZT film sintering temperature, in this work, the effect of the powder size and sintering aid on the sintering temperature was studied. A maskless, CAD driven, non-contact direct printing system, aerosol jet printer, was used to deposit PZT thick films on the substrate. This technique allows creating features without masking and etching processes that are generally required for realizing designed features via conventional deposition approaches. Broadband, sub-millisecond, high intensity flash pulses were used to sinter the PZT films. The role of all sintering parameters was investigated to regulate the sintering quality of the PZT thick films. The photonically sintered films showed much lower substrate temperature increase mainly due to the extremely short pulse duration and temperature gradient through the film thickness. The superior piezoelectric property to thermally sintered group was also obtained. This process significantly shortens the processing duration and dramatically expands the possible substrate materials. It accordingly opens the possibility of processing PZT film directly on low melting point materials. A PZT energy harvester based on this process was directly fabricated on the polyethylene terephthalate (PET) substrate to demonstrate the capability. The relation between the load and the generated power was investigated to obtain the highest output power. Up to 0.1 μW was generated from this flexible energy harvester when connected with 10 MΩ resistive load. Photonic sintering of PZT film also creates the opportunity of processing poling during sintering. Different combinations of the sintering and poling techniques were studied. It was observed that the best piezoelectric property was obtained while performing poling during photonic sintering. Consequently, a new method of printing, sintering, and poling of micro-scaled PZT films was demonstrated in this work resulting in high performance films. This process provides the capability of realizing PZT devices on low temperature substrate, facilitates the fabrication of flexible piezoelectric devices, and enhances the piezoelectric property

Advanced sensors technology survey

This project assesses the state-of-the-art in advanced or 'smart' sensors technology for NASA Life Sciences research applications with an emphasis on those sensors with potential applications on the space station freedom (SSF). The objectives are: (1) to conduct literature reviews on relevant advanced sensor technology; (2) to interview various scientists and engineers in industry, academia, and government who are knowledgeable on this topic; (3) to provide viewpoints and opinions regarding the potential applications of this technology on the SSF; and (4) to provide summary charts of relevant technologies and centers where these technologies are being developed

Piezo-electromechanical smart materials with distributed arrays of piezoelectric transducers: Current and upcoming applications

This review paper intends to gather and organize a series of works which discuss the possibility of exploiting the mechanical properties of distributed arrays of piezoelectric transducers. The concept can be described as follows: on every structural member one can uniformly distribute an array of piezoelectric transducers whose electric terminals are to be connected to a suitably optimized electric waveguide. If the aim of such a modification is identified to be the suppression of mechanical vibrations then the optimal electric waveguide is identified to be the 'electric analog' of the considered structural member. The obtained electromechanical systems were called PEM (PiezoElectroMechanical) structures. The authors especially focus on the role played by Lagrange methods in the design of these analog circuits and in the study of PEM structures and we suggest some possible research developments in the conception of new devices, in their study and in their technological application. Other potential uses of PEMs, such as Structural Health Monitoring and Energy Harvesting, are described as well. PEM structures can be regarded as a particular kind of smart materials, i.e. materials especially designed and engineered to show a specific andwell-defined response to external excitations: for this reason, the authors try to find connection between PEM beams and plates and some micromorphic materials whose properties as carriers of waves have been studied recently. Finally, this paper aims to establish some links among some concepts which are used in different cultural groups, as smart structure, metamaterial and functional structural modifications, showing how appropriate would be to avoid the use of different names for similar concepts. © 2015 - IOS Press and the authors

Piezoelectric Vibration Damping of Rolling Contact

Machines, which contain heavy rotating objects like rolls, are always sensitive to vibrations. These vibrations can usually be limited by a conservative design approach, by carefully balancing the rolls and by applying high precise manufacturing techniques. More critical are machines, in which rolls are in direct rolling contact and a web-like thin material is fed through this contact. Such material manipulation is used for example in manufacturing of paper, thin foils and metal sheets or in rotary printing machines. In the first case the rolls today are covered by polymer materials in order to make the contact zone larger. This produces non-classical delay type resonances, when the roll cover is deformed in the contact zone, and this penetration profile is entering the contact zone again before complete recovery. This type of self-excited nonlinear vibration is difficult to control with purely traditional damping methods. Active damping methods bring more possibilities to adapt to different running conditions. The knowledge of existing delay-resonance cases calls for methods, actuators and control circuits, which have the required performance to move rolls of 10 tons mass at frequency band 100 Hz and peak-amplitude level 0,01 mm. After closing out many other possibilities, piezoelectric actuators have been proposed to such damping task and the purpose of this thesis is to evaluate the feasibility of commercially available actuators in this service. Piezoelectric actuators are very promising for vibration control applications, because of their easy controllability, high performance in producing large magnitude forces in combination with small magnitude motion outputs in an extremely fast response time. The control is straightforward by simply variating the input voltage of the actuator. Classical damping approaches are bringing the possibility to utilize large control gains in a wide stability domain. When control voltage is generated based on the vibration data measured from the system, which is the case in active damping approach, a counter-force driven by the piezoactuator can be fed in the opposite phase to the vibrating system. It is also possible to build a passive vibration damper by connecting an electric circuit to the electrodes of the piezoelectric actuator in order to harvest the electric energy originating from the oscillating mechanical part of the system. These electric circuits can consist of a resistor, an inductor and a capacitance in different serial or parallel layouts. In order to make such circuit adaptive one, sophisticated control electronics is needed to on-line modify the adjustable circuit parameters

Photoacoustic Detection of Terahertz Radiation for Chemical Sensing and Imaging Applications

The main research objective is the development of photoacoustic sensor capable of detecting weak terahertz (THz) electromagnetic radiation. The feasibility of THz remote sensing is seen in the utilization of Microelectromechanical systems (MEMS) cantilever-based sensor. The overall sensing functionality of the detector in development is based on the photoacoustic spectroscopy and direct piezoelectric effect phenomena, as a result of which significant part of investigation has been conducted in the areas of terahertz electromagnetic radiation and its detection. The main focus of this research work was the detector analytical and Finite Element Method (FEM) simulation modeling, involving necessary material properties investigations and adequate selections which were, beside the sensors\u27 geometry considerations, heavily engaged in the device modeling. Five different MEMS detector configurations have been analyzed and modeled as potential THz photoacoustic sensing options: Three configurations of rectangular shape, single piezoelectric layer cantilever-based sensors, Circular membrane sensing configuration and Square membrane sensing configuration. Some level of disagreement was discovered between the analytical and FEM simulated results, which has been analyzed and possible reasons were established. The obtained results indicated that the Square membrane has demonstrated the ability to respond effectively to any radiation level from the entire THz photoacoustic range exhibiting high sensitivity and thus was selected as the best terahertz photoacoustic sensing solution