Adaptive Optics for Astronomy

Adaptive Optics is a prime example of how progress in observational astronomy can be driven by technological developments. At many observatories it is now considered to be part of a standard instrumentation suite, enabling ground-based telescopes to reach the diffraction limit and thus providing spatial resolution superior to that achievable from space with current or planned satellites. In this review we consider adaptive optics from the astrophysical perspective. We show that adaptive optics has led to important advances in our understanding of a multitude of astrophysical processes, and describe how the requirements from science applications are now driving the development of the next generation of novel adaptive optics techniques.Comment: to appear in ARA&A vol 50, 201

Point spread function modelling for astronomical telescopes: a review focused on weak gravitational lensing studies

The accurate modelling of the Point Spread Function (PSF) is of paramount importance in astronomical observations, as it allows for the correction of distortions and blurring caused by the telescope and atmosphere. PSF modelling is crucial for accurately measuring celestial objects' properties. The last decades brought us a steady increase in the power and complexity of astronomical telescopes and instruments. Upcoming galaxy surveys like Euclid and LSST will observe an unprecedented amount and quality of data. Modelling the PSF for these new facilities and surveys requires novel modelling techniques that can cope with the ever-tightening error requirements. The purpose of this review is three-fold. First, we introduce the optical background required for a more physically-motivated PSF modelling and propose an observational model that can be reused for future developments. Second, we provide an overview of the different physical contributors of the PSF, including the optic- and detector-level contributors and the atmosphere. We expect that the overview will help better understand the modelled effects. Third, we discuss the different methods for PSF modelling from the parametric and non-parametric families for ground- and space-based telescopes, with their advantages and limitations. Validation methods for PSF models are then addressed, with several metrics related to weak lensing studies discussed in detail. Finally, we explore current challenges and future directions in PSF modelling for astronomical telescopes.Comment: 63 pages, 14 figures. Submitte

Learning Wavefront Coding for Extended Depth of Field Imaging

Depth of field is an important factor of imaging systems that highly affects the quality of the acquired spatial information. Extended depth of field (EDoF) imaging is a challenging ill-posed problem and has been extensively addressed in the literature. We propose a computational imaging approach for EDoF, where we employ wavefront coding via a diffractive optical element (DOE) and we achieve deblurring through a convolutional neural network. Thanks to the end-to-end differentiable modeling of optical image formation and computational post-processing, we jointly optimize the optical design, i.e., DOE, and the deblurring through standard gradient descent methods. Based on the properties of the underlying refractive lens and the desired EDoF range, we provide an analytical expression for the search space of the DOE, which is instrumental in the convergence of the end-to-end network. We achieve superior EDoF imaging performance compared to the state of the art, where we demonstrate results with minimal artifacts in various scenarios, including deep 3D scenes and broadband imaging

Astigmatism and Pseudoaccommodation in Pseudophakic Eyes

noAdvanced IOLs with circumferential zones of different power provide pseudoaccommodation. We investigated the potential for power variation with meridian, namely astigmatism, to provide pseudo-accommodation. With appropriate power and axis orientations, acceptable pseudo-accommodation can be achieved

Resolution Study of a Hyperspectral Sensor Using Computed Tomography in the Process of Noise

Recently, a new type of hyperspectral imaging sensor has been proposed which simultaneously records both spectral data and multiple spatial dimensions. Unlike dispersive imaging spectrometers, chromo-tomographic hyperspectral imaging sensors (CTHIS) record two spatial dimensions as well as a spectral dimension using computed tomography (CT) techniques with only a finite number of spatially-spectrally diverse images. To date, the factors affecting resolution of these sensors have not been examined. This research examines factors affecting resolution, specifically the number of the focus planes needed to resolve a particular object calculated from a theoretical lower bound, determine a method of reconstructing a hyperspectral object in the presence of noise and background and verify the proposed method of reconstruction and the lower bound applying the proposed reconstruction method to laboratory data. Finally, a simple method is proposed and tested to use this sensor in the presence of atmospheric turbulence with a modified reconstructor to blindly estimate the seeing parameter

Remote refocusing light-sheet fluorescence microscopy for high-speed 2D and 3D imaging of calcium dynamics in cardiomyocytes

The high prevalence and poor prognosis of heart failure are two key drivers for research into cardiac electrophysiology and regeneration. Dyssynchrony in calcium release and loss of structural organization within individual cardiomyocytes (CM) has been linked to reduced contractile strength and arrhythmia. Correlating calcium dynamics and cell microstructure requires multidimensional imaging with high spatiotemporal resolution. In light-sheet fluorescence microscopy (LSFM), selective plane illumination enables fast optically sectioned imaging with lower phototoxicity, making it suitable for imaging subcellular dynamics. In this work, a custom remote refocusing LSFM system is applied to studying calcium dynamics in isolated CM, cardiac cell cultures and tissue slices. The spatial resolution of the LSFM system was modelled and experimentally characterized. Simulation of the illumination path in Zemax was used to estimate the light-sheet beam waist and confocal parameter. Automated MATLAB-based image analysis was used to quantify the optical sectioning and the 3D point spread function using Gaussian fitting of bead image intensity distributions. The results demonstrated improved and more uniform axial resolution and optical sectioning with the tighter focused beam used for axially swept light-sheet microscopy. High-speed dual-channel LSFM was used for 2D imaging of calcium dynamics in correlation with the t-tubule structure in left and right ventricle cardiomyocytes at 395 fps. The high spatio-temporal resolution enabled the characterization of calcium sparks. The use of para-nitro-blebbistatin (NBleb), a non-phototoxic, low fluorescence contraction uncoupler, allowed 2D-mapping of the spatial dyssynchrony of calcium transient development across the cell. Finally, aberration-free remote refocusing was used for high-speed volumetric imaging of calcium dynamics in human induced pluripotent stem-cell derived cardiomyocytes (hiPSC-CM) and their co-culture with adult-CM. 3D-imaging at up to 8 Hz demonstrated the synchronization of calcium transients in co-culture, with increased coupling with longer co-culture duration, uninhibited by motion uncoupling with NBleb.Open Acces