Magnetic microrobot control using an adaptive fuzzy sliding-mode method

The magnetic medical microrobots are influenced by diverse factors such as the medium, the geometry of the microrobot, and the imaging procedure. It is worth noting that the size limitations make it difficult or even impossible to obtain reliable physical properties of the system. In this research, to achieve a precise microrobot control using minimum knowledge about the system, an Adaptive Fuzzy Sliding-Mode Control (AFSMC) scheme is designed for the motion control problem of the magnetically actuated microrobots in presence of input saturation constraint. The AFSMC input consists of a fuzzy system designed to approximate an unknown nonlinear dynamical system and a robust term considered for mismatch compensation. According to the designed adaptation laws, the asymptotic stability is proved based on the Lyapunov theorem and Barbalat's lemma. In order to evaluate the effectiveness of the proposed method, a comparative simulation study is conducted

A Magnetically and Electrically Powered Hybrid Micromotor in Conductive Solutions: Synergistic Propulsion Effects and Label-Free Cargo Transport and Sensing

Electrically powered micro- and nanomotors are promising tools for in-vitro single-cell analysis. In particular, single cells can be trapped, transported and electroporated by a Janus particle (JP) using an externally applied electric field. However, while dielectrophoretic (DEP)-based cargo manipulation can be achieved at high-solution conductivity, electrical propulsion of these micromotors becomes ineffective at solution conductivities exceeding 0.3mS/cm. Here, we successfully extended JP cargo manipulation and transport capabilities to conductive near-physiological (<6mS/cm) solutions by combining magnetic field-based micromotor propulsion and navigation with DEP-based manipulation of various synthetic and biological cargos. Combination of a rotating magnetic field and electric field resulted in enhanced micromotor mobility and steering control through tuning of the electric field frequency. conditions are necessary. In addition, we demonstrated the micromotors ability of identifying apoptotic cell among viable and necrotic cells based their dielectrophoretic difference, thus, enabling to analyze the apoptotic status in the single cell samples for drug discovery, cell therapeutics and immunotherapy. We also demonstrated the ability to trap and transport live cells towards regions containing doxorubicin-loaded liposomes. This hybrid micromotor approach for label-free trapping, transporting and sensing of selected cells within conductive solutions, opens new opportunities in drug delivery and single cell analysis, where close-to-physiological medi

A magnetically actuated microrobot for targeted neural cell delivery and selective connection of neural networks

There has been a great deal of interest in the development of technologies for actively manipulating neural networks in vitro, providing natural but simplified environments in a highly reproducible manner in which to study brain function and related diseases. Platforms for these in vitro neural networks require precise and selective neural connections at the target location, with minimal external influences, and measurement of neural activity to determine how neurons communicate. Here, we report a neuron-loaded microrobot for selective connection of neural networks via precise delivery to a gap between two neural clusters by an external magnetic field. In addition, the extracellular action potential was propagated from one cluster to the other through the neurons on the microrobot. The proposed technique shows the potential for use in experiments to understand how neurons communicate in the neural network by actively connecting neural clusters. Copyright © 2020 The Authors, some rights reserved.1

A Method for High Resolution PEGDA Hydrogel Photopatterning and Hydrogel Release in Microfluidic Devices

Soft microrobots are broadly applied in biomedical applications, including biosensing, diagnostics, drug delivery, and cell manipulation. There has been growing interest in automatic microfabrication of reliable and robust small scale hydrogels using the digital maskless photolithographic patterning method. In this thesis, we investigated the factors influencing photopatterning capability and efficiency in microfluidics, and we devised a method of releasing hydrogel patterns selectively for further applications. We applied benzophenone pretreatment of PDMS devices to form an oxygen quenching region surrounding the microfluidic channel to reduce the effect of oxygen inhibition. In addition, we made some optimizations to enhance the photopatterning efficiency in microfluidics, including adding a stopper to achieve stop-flow lithography, avoiding phase separation by introducing water soluble photoinitiator, and analyzing the effect of UV curing time on hydrogel sizes. In order to release the hydrogel patterns selectively, we utilized poly (acrylic acid) as the sacrificial layer, where its water solubility was controlled by Ca2+ ion and Na+ ion concentrations. We also designed a new method of assembling microfluidics by attaching two PDMS channels together and curing the sacrificial layer before device assembly for better lift-off specificity in microfluidics

Multifunctional microrobot with real-time visualization and magnetic resonance imaging for chemoembolization therapy of liver cancer

Microrobots that can be precisely guided to target lesions have been studied for in vivo medical applications. However, existing microrobots have challenges in vivo such as biocompatibility, biodegradability, actuation module, and intra- and postoperative imaging. This study reports microrobots visualized with real-time x-ray and magnetic resonance imaging (MRI) that can be magnetically guided to tumor feeding vessels for transcatheter liver chemoembolization in vivo. The microrobots, composed of a hydrogel-enveloped porous structure and magnetic nanoparticles, enable targeted delivery of therapeutic and imaging agents via magnetic guidance from the actuation module under real-time x-ray imaging. In addition, the microrobots can be tracked using MRI as postoperative imaging and then slowly degrade over time. The in vivo validation of microrobot system-mediated chemoembolization was demonstrated in a rat liver with a tumor model. The proposed microrobot provides an advanced medical robotic platform that can overcome the limitations of existing microrobots and current liver chemoembolization.ope

Custom-Designed Biohybrid Micromotor for Potential Disease Treatment

Micromotors are recognized as promising candidates for untethered micromanipulation and targeted cargo transport. Their future application is, however, hindered by the low efficiency of drug encapsulation and their poor adaptability in physiological conditions. To address these challenges, one potential solution is to incorporate micromotors with biological materials as the combination of functional biological entities and smart artificial parts represents a manipulable and biologically friendly approach. This dissertation focuses on the development of custom-designed micromotors combined with sperm and their potential applications on targeted diseases treatment. By means of 2D and 3D lithography methods, microstructures with complex configurations can be fabricated for specific demands. Bovine and human sperm are both for the first time explored as drug carriers thanks to their high encapsulation efficiency of hydrophilic drugs, their powerful self-propulsion and their improved drug-uptake relying on the somatic-cell fusion ability. The hybrid micromotors containing drug loaded sperm and constructed artificial enhancements can be self-propelled by the sperm flagella and remotely guided and released to the target at high precision by employing weak external magnetic fields. As a result, micromotors based on both bovine and human sperm show significant anticancer effect. The application here can be further broadened to other biological environments, in particular to the blood stream, showing the potential on the treatment of blood diseases like blood clotting. Finally, to enhance the treatment efficiency, in particular to control sperm number and drug dose, three strategies are demonstrated to transport swarms of sperm. This research paves the way for the precision medicine based on engineered sperm-based micromotors

Magnetically actuated microrobots as a platform for stem cell transplantation

Magnetic microrobots were developed for three-dimensional culture and the precise delivery of stem cells in vitro, ex vivo, and in vivo. Hippocampal neural stem cells attached to the microrobots proliferated and differentiated into astrocytes, oligodendrocytes, and neurons. Moreover, microrobots were used to transport colorectal carcinoma cancer cells to tumor microtissue in a body-on-a-chip, which comprised an in vitro liver-tumor microorgan network. The microrobots were also controlled in a mouse brain slice and rat brain blood vessel. Last, microrobots carrying mesenchymal stem cells derived from human nose were manipulated inside the intraperitoneal cavity of a nude mouse. The results indicate the potential of microrobots for the culture and delivery of stem cells. Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works1