Brain and Human Body Modeling

This open access book describes modern applications of computational human modeling with specific emphasis in the areas of neurology and neuroelectromagnetics, depression and cancer treatments, radio-frequency studies and wireless communications. Special consideration is also given to the use of human modeling to the computational assessment of relevant regulatory and safety requirements. Readers working on applications that may expose human subjects to electromagnetic radiation will benefit from this book’s coverage of the latest developments in computational modelling and human phantom development to assess a given technology’s safety and efficacy in a timely manner. Describes construction and application of computational human models including anatomically detailed and subject specific models; Explains new practices in computational human modeling for neuroelectromagnetics, electromagnetic safety, and exposure evaluations; Includes a survey of modern applications for which computational human models are critical; Describes cellular-level interactions between the human body and electromagnetic fields

Doctor of Philosophy

dissertationMagnetic resonance-guided focused ultrasound surgery (MRgFUS) is a noninvasive means of causing selective tissue necrosis using high-power ultrasound and MR temperature imaging. Inhomogeneities in the medium of propagation can cause significant distortion of the ultrasound beam, resulting in changes in focal-zone amplitude, location and shape. Current ultrasound beam simulation techniques are either only applicable to homogeneous media or are relatively slow in calculating power deposition patterns in inhomogeneous media. Further, these techniques use table-value estimates of the acoustic parameters for predicting ultrasound beam propagation in inhomogeneous media, resulting in at best an approximate power deposition pattern. This work improves numerical analysis of ultrasound beam propagation by developing techniques for: 1) fast, accurate predictions of ultrasound beam propagation in inhomogeneous media, 2) noninvasive estimation of acoustic parameters (speed of sound and attenuation coefficient) of tissue types present in inhomogeneous media, 3) noninvasive determination of changes in tissue acoustic properties due to treatment. These beam simulation techniques utilizing subject-specific tissue parameters will rapidly predict power deposition patterns in real patient geometries and estimate changes in tissue acoustic parameters during treatment, leading to treatment-responsive patientspecific treatment plans that will improve the safety, efficacy and effectiveness of MRgFUS

Brain and Human Body Modeling

This open access book describes modern applications of computational human modeling with specific emphasis in the areas of neurology and neuroelectromagnetics, depression and cancer treatments, radio-frequency studies and wireless communications. Special consideration is also given to the use of human modeling to the computational assessment of relevant regulatory and safety requirements. Readers working on applications that may expose human subjects to electromagnetic radiation will benefit from this book’s coverage of the latest developments in computational modelling and human phantom development to assess a given technology’s safety and efficacy in a timely manner. Describes construction and application of computational human models including anatomically detailed and subject specific models; Explains new practices in computational human modeling for neuroelectromagnetics, electromagnetic safety, and exposure evaluations; Includes a survey of modern applications for which computational human models are critical; Describes cellular-level interactions between the human body and electromagnetic fields

SAR Prediction and SAR Management for Parallel Transmit MRI

Parallel transmission enables control of the RF field in high-field Magnetic Resonance Imaging (MRI). However, the approach has also caused concerns about the specific absorption rate (SAR) in the patient body. The present work provides new concepts for SAR prediction. A novel approach for generating human body models is proposed, based on a water-fat separated MRI pre-scan. Furthermore, this work explores various approaches for SAR reduction

Design and optimisation of radio-frequency probes for high field magnetic imaging

This thesis addresses the inhomogeneity and the high SAR values associated with the state-of-art 7 T high field MR system. The high signal to noise ratio associated with such high field systems ≥ 7 T is a continuous driver to use such systems where high resolution images are acquired at short acquisition time. However, these systems come with many challenges. The central brightening effect in MR images indicates a B1 degraded field uniformity. For example, at 7 T with an operational electromagnetic frequency of 298 MHz the wavelength is about 12 cm in tissues. At such short wavelengths, circuit and electromagnetic theory will be invalid an analytical solution and is no longer feasible to predict magnetic field distribution. In this thesis the full wave method, Transmission Line Modelling (TLM) technique is used in conjunction with Tikhonov regularisation inverse method in order to optimise phases and amplitude of elementary drive currents of four different coils for optimal uniformity and low SAR values. Two dimensional 8 and 16 rungs birdcage-like coils were first optimised. Then the optimisation was carried out for the three dimensional problem for 8 and 16 rungs birdcage-like coils and then compared to 32 and 64 multi-element coils. The travelling wave approach is a recent approach to overcome field inhomogeneity and high SAR values. An Antenna is used to couple head/body to a travelling wave RF signal. Using Finite Difference Time Domain (FDTD) method, a patch antenna has been designed, and the effect of using matching load for maximising the power flow in the magnet bore, field uniformity and reducing SAR values in the head have been explored. An end tapered waveguide has been designed for local imaging and tested on the 7 T Philips Acheiva system. Further developments have been suggested for the end tapered waveguide by suggesting the design of a dielectric transformer. It is envisaged that the waveguide approach is ideally suited for a multi-transmit system which would employ a number of waveguide ports

ADVANCED INTRAVASCULAR MAGNETIC RESONANCE IMAGING WITH INTERACTION

Intravascular (IV) Magnetic Resonance Imaging (MRI) is a specialized class of interventional MRI (iMRI) techniques that acquire MRI images through blood vessels to guide, identify and/or treat pathologies inside the human body which are otherwise difficult to locate and treat precisely. Here, interactions based on real-time computations and feedback are explored to improve the accuracy and efficiency of IVMRI procedures. First, an IV MRI-guided high-intensity focused ultrasound (HIFU) ablation method is developed for targeting perivascular pathology with minimal injury to the vessel wall. To take advantage of real-time feedback, a software interface is developed for monitoring thermal dose with real-time MRI thermometry, and an MRI-guided ablation protocol developed and tested on muscle and liver tissue ex vivo. It is shown that, with cumulative thermal dose monitored with MRI thermometry, lesion location and dimensions can be estimated consistently, and desirable thermal lesions can be achieved in animals in vivo. Second, to achieve fully interactive IV MRI, high-resolution real-time 10 frames-per-second (fps) MRI endoscopy is developed as an advance over prior methods of MRI endoscopy. Intravascular transmit-receive MRI endoscopes are fabricated for highly under-sampled radial-projection MRI in a clinical 3Tesla MRI scanner. Iterative nonlinear reconstruction is accelerated using graphics processor units (GPU) to achieve true real-time endoscopy visualization at the scanner. The results of high-speed MRI endoscopy at 6-10 fps are consistent with fully-sampled MRI endoscopy and histology, with feasibility demonstrated in vivo in a large animal model. Last, a general framework for automatic imaging contrast tuning over MRI protocol parameters is explored. The framework reveals typical signal patterns over different protocol parameters from calibration imaging data and applies this knowledge to design efficient acquisition strategies and predicts contrasts under unacquired protocols. An external computer in real-time communication with the MRI console is utilized for online processing and controlling MRI acquisitions. This workflow enables machine learning for optimizing acquisition strategies in general, and provides a foundation for efficiently tuning MRI protocol parameters to perform interventional MRI in the highly varying and interactive environments commonly in play. This work is loosely inspired by prior research on extremely accelerated MRI relaxometry using the minimal-acquisition linear algebraic modeling (SLAM) method

A study on the change in plasma membrane potential during sonoporation

Posters: no. 4Control ID: 1680329OBJECTIVES: There has been validated that the correlation of sonoporation with calcium transients is generated by ultrasound-mediated microbubbles activity. Besides calcium, other ionic flows are likely involved in sonoporation. Our hypothesis is the cell electrophysiological properties are related to the intracellular delivery by ultrasound and microbubbles. In this study, a real-time live cell imaging platform is used to determine whether plasma membrane potential change is related to the sonoporation process at the cellular level. METHODS: Hela cells were cultured in DMEM supplemented with 10% FBS in Opticell Chamber at 37 °C and 5% CO2, and reached 80% confluency before experiments. The Calcein Blue-AM, DiBAC4(3) loaded cells in the Opticell chamber filled with PI solution and Sonovue microbubbles were immerged in a water tank on a inverted fluorescence microscope. Pulsed ultrasound (1MHz freq., 20 cycles, 20Hz PRF, 0.2-0.5MPa PNP) was irradiated at the angle of 45° to the region of interest for 1s.The real-time fluorescence imaging for different probes was acquired by a cooled CCD camera every 20s for 10min. The time-lapse fluorescence images were quantitatively analyzed to evaluate the correlation of cell viability, intracellular delivery with plasma membrane potential change. RESULTS: Our preliminary data showed that the PI fluorescence, which indicated intracellular delivery, was immediately accumulated in cells adjacent to microbubbles after exposure, suggesting that their membranes were damaged by ultrasound-activated microbubbles. However, the fluorescence reached its highest level within 4 to 6 minutes and was unchanged thereafter, indicating the membrane was gradually repaired within this period. Furthermore, using DIBAC4(3), which detected the change in the cell membrane potential, we found that the loss of membrane potential might be associated with intracellular delivery, because the PI fluorescence accumulation was usually accompanied with the change in DIBAC4 (3) fluorescence. CONCLUSIONS: Our study suggests that there may be a linkage between the cell membrane potential change and intracellular delivery mediated by ultrasound and microbubbles. We also suggest that other ionic flows or ion channels may be involved in the cell membrane potential change in sonoporation. Further efforts to explore the cellular mechanism of this phenomenon will improve our understanding of sonoporation.postprin