Under which conditions is carrier cooperation possible? A case study in a Seville marketplace

The high volume of traffic originates two well-known problems in many cities: congestion and pollution. In recent years, a social phenomenon is emerging cooperation. This work is aimed at evaluating the circumstances under which transport cooperation is possible between different stakeholders operating in the same geographical area. To this end, a double survey process was conducted in a marketplace situated in the Seville City (Spain) centre. The first survey was designed to know the characteristics of the retailers and their preferences with respect to cooperation and regulations. A relational analysis between retailer features and their willingness to cooperate was carried out. After analysing the motivations for non-cooperation, a mixed proposal was designed and surveyed. Although the research was limited to a marketplace, the relevant data gathered from this double survey process highlights some implications: (a) the importance of personal relations in retailer cooperation; (b) a high volume of freight and the use of vans as on-street warehouses appear as significant motivations for non-cooperation; (c) forcing changes in the statu quo encourages cooperation.Ministerio de Economía y Competitividad (España) TEC2013-47286-C3-3-

Value Chain: From iDMU to Shopfloor Documentation of Aeronautical Assemblies

Competition in the aerospace manufacturing companies has led them to continuously improve the efficiency of their processes from the conceptual phase to the start of production and during operation phase, providing services to clients. PLM (Product Lifecycle Management) is an end-to-end business solution which aims to provide an environment of information about the product and related processes available to the whole enterprise throughout the product’s lifecycle. Airbus designs and industrializes aircrafts using Concurrent Engineering methods since decades. The introduction of new PLM methods, procedures and tools, and the need to improve processes efficiency and reduce time-to-market, led Airbus to pursue the Collaborative Engineering method. Processes efficiency is also impacted by the variety of systems existing within Airbus. Interoperability rises as a solution to eliminate inefficiencies due to information exchange and transformations and it also provides a way to discover and reuse existing information. The ARIADNE project (Value chain: from iDMU to shopfloor documentation of aeronautical assemblies) was launched to support the industrialization process of an aerostructure by implementing the industrial Digital Mock-Up (iDMU) concept in a Collaborative Engineering framework. Interoperability becomes an important research workpackage in ARIADNE to exploit and reuse the information contained in the iDMU and to create the shop floor documentation. This paper presents the context, the conceptual approach, the methodology adopted and preliminary results of the project

Human oocytes and zygotes are ready for ultra-fast vitrification after 2 minutes of exposure to standard CPA solutions

Vitrification of human oocytes and embryos in different stages of development is a key element of daily clinical practice of in vitro fertilization treatments. Despite the cooling and warming of the cells is ultra-fast, the procedure as a whole is time consuming. Most of the duration is employed in a long (8–15 minutes), gradual or direct exposure to a non-vitrifying cryoprotectant solution, which is followed by a short exposure to a more concentrated vitrifying solution. A reduction in the duration of the protocols is desirable to improve the workflow in the IVF setting and reduce the time of exposure to suboptimal temperature and osmolarity, as well as potentially toxic cryoprotectants. In this work it is shown that this reduction is feasible. In silico (MatLab program using two-parameter permeability model) and in vitro observations of the oocytes’ osmotic behaviour indicate that the dehydration upon exposure to standard cryoprotectant solutions occurs very fast: the point of minimum volume of the shrink-swell curve is reached within 60 seconds. At that point, intracellular water ejection is complete, which coupled with the permeation of low molecular weight cryoprotectants results in similar intracellular and extracellular solute concentrations. This shows that prolonging the exposure to the cryoprotectant solutions does not improve the cytosolic glass forming tendency and could be avoided. To test this finding, human oocytes and zygotes that were donated for research were subjected to a shortened, dehydration-based protocol, consisting of two consecutive exposures of one-minute to two standard cryoprotectant solutions, containing ethylene glycol, dimethyl sulfoxide and sucrose. At the end of this two-minute dehydration protocol, the critical intracellular solute concentration necessary for successful vitrification was attained, confirmed by the post-warming survival and ability to resume cytokinesis of the cells. Further studies of the developmental competency of oocytes and embryos would be necessary to determine the suitability of this specific dehydration protocol for clinical practice, but based on our results, short times of exposure to increasingly hypertonic solutions could be a more time-efficient strategy to prepare human oocytes and embryos for vitrification

Stepped vitrification technique for human ovarian tissue cryopreservation

The advantage of stepped vitrification (SV) is avoiding ice crystal nucleation, while decreasing the toxic effects of high cryoprotectant concentrations. We aimed to test this method for human ovarian tissue cryopreservation. Ovarian cortex was taken from 7 fertile adult women. Samples were subjected to an SV protocol performed in an automatic freezer, which allowed sample transfer to ever higher concentrations of dimethyl sulfoxide (DMSO) as the temperature was reduced. Histological evaluation of the vitrified-warmed tissue showed large numbers of degenerated follicles after 24 hours of in vitro culture. We therefore evaluated DMSO perfusion rates by X-ray computed tomography, ice crystal formation by freeze-substitution, and cell toxicity by transmission electron microscopy, seeking possible reasons why follicles degenerated. Although cryoprotectant perfusion was considered normal and no ice crystals were formed in the tissue, ultrastructural analysis detected typical signs of DMSO toxicity, such as mitochondria degeneration, alterations in chromatin condensation, cell vacuolization and extracellular matrix swelling in both stromal and follicular cells. The findings indicated that the method failed to preserve follicles due to the high concentrations of DMSO used. However, adaptations can be made to avoid toxicity to follicles caused by elevated levels of cryoprotectants.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) 2016/22947-

Limitations of the Standard Procedure for the Evaluation of Marble and Limestone for Use in Construction: A Critical Analysis and Proposal for Modification

The selection of natural stone for each specific use must be based on determinations which assess their technical quality and ensure their suitability for the environment in which they will be utilized. Among the criteria to be considered, a petrographic characterization is basic to deduce the behaviour of the stone to external agents. Current European regulations for the valuation and use of a stone present serious constraints in connection with the petrographic characterization and with the definition of some of the technological tests, which can endanger the suitability of a stone for use in construction. Two commercial limestones and one marble, are studied following the existing European Standards, to explain reasonably the behavior of these materials and deduce the most appropriate uses. Finally the results lead to certain recommendations to modify existing regulations

Promoting Intermodal Connectivity at California’s High Speed Rail Stations

High-speed rail (HSR) has emerged as one of the most revolutionary and transformative transportation technologies, having a profound impact on urban-regional accessibility and inter-city travel across Europe, Japan, and more recently China and other Asian countries. One of HSR’s biggest advantages over air travel is that it offers passengers a one-seat ride into the center of major cities, eliminating time-consuming airport transfers and wait times, and providing ample opportunities for intermodal transfers at these locales. Thus, HSR passengers are typically able to arrive at stations that are only a short walk away from central business districts and major tourist attractions, without experiencing any of the stress that car drivers often experience in negotiating such highly congested environments. Such an approach requires a high level of coordination and planning of the infrastructural and spatial aspects of the HSR service, and a high degree of intermodal connectivity. But what key elements can help the US high-speed rail system blend successfully with other existing rail and transit services? That question is critically important now that high-speed rail is under construction in California. The study seeks to understand the requirements for high levels of connectivity and spatial and operational integration of HSR stations and offer recommendations for seamless, and convenient integrated service in California intercity rail/HSR stations. The study draws data from a review of the literature on the connectivity, intermodality, and spatial and operational integration of transit systems; a survey of 26 high-speed rail experts from six different European countries; and an in-depth look of the German and Spanish HSR systems and some of their stations, which are deemed as exemplary models of station connectivity. The study offers recommendations on how to enhance both the spatial and the operational connectivity of high-speed rail systems giving emphasis on four spatial zones: the station, the station neighborhood, the municipality at large, and the region

New Eunicellin-Type Diterpenes from the Panamanian Octocoral Briareum Asbestinum

Gorgonian octocorals are considered a prolific source of secondary metabolites with a wide range of biological activities, including anti-inflammatory activity. In particular, the genus Briareum is known for producing a wealth of diterpenes with complex chemical structures. The chemical study of the methanolic extract of Briareum asbestinum collected in Bocas del Toro, on the Caribbean side of Panama, led to the isolation of three new eunicellin-type diterpenes: briarellin T (1), asbestinin 27 (2), asbestinin 28 (3) and the previously described asbestinin 17 (4). The structures of the new compounds were determined by extensive NMR analyses and HRMS. Anti-inflammatory activity assays showed a significant reduction of the pro-inflammatory cytokines TNF-α, IL-6, IL-1β and IL-8 as well as a downregulation of COX-2 expression in LPS-stimulated THP-1 macrophages. These findings support the potential use of these marine compounds as therapeutic agents in the treatment of inflammatory diseases

Models of public-private partnerships in megaprojects: the Spanish case

This article provides a literature review of PPP Models, where the clarification of this current confusion and ambiguity constitute the fundamental issue addressed by our research. The systematization of the PPP models is performed by applying six classification criteria based on organizational and financial aspects and focused on the Spanish experience. Additionally, a comparative study of the various schemes applied in European countries is carried out, whereby the concession model implemented successfully in Spain is studied in greater detail. To this end, a megaproject, the first metro line of Seville (Spain) forms the basis of a case-study. When the megaproject is viable through user fees, the public sector can use PPPs to defer payments and as a way to control their deficits and debt without cutting investments in infrastructures and public services. Nevertheless, certain drawbacks should be borne in mind, such as the expenditure commitments of future budgets, the higher cost of private funding, and the necessity for transparency and accountability of PPP contractual arrangements to be improved. Therefore, the aim of this article is to analyze the various forms of PPPs in megaprojects in order to determine the potential efficiency gains that can be achieved in the implementation of these models

Nonlinear model predictive control for thermal management in plug-in hybrid electric vehicles

© 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.A nonlinear model predictive control (NMPC) for the thermal management (TM) of Plug-in Hybrid Electric Vehicles (PHEVs) is presented. TM in PHEVs is crucial to ensure good components performance and durability in all possible climate scenarios. A drawback of accurate TM solutions is the higher electrical consumption due to the increasing number of low voltage (LV) actuators used in the cooling circuits. Hence, more complex control strategies are needed for minimizing components thermal stress and at the same time electrical consumption. In this context, NMPC arises as a powerful method for achieving multiple objectives in Multiple input- Multiple output systems. This paper proposes an NMPC for the TM of the High Voltage (HV) battery and the power electronics (PE) cooling circuit in a PHEV. It distinguishes itself from the previously NMPC reported methods in the automotive sector by the complexity of its controlled plant which is highly nonlinear and controlled by numerous variables. The implemented model of the plant, which is based on experimental data and multi- domain physical equations, has been validated using six different driving cycles logged in a real vehicle, obtaining a maximum error, in comparison with the real temperatures, of 2C. For one of the six cycles, an NMPC software-in-the loop (SIL) is presented, where the models inside the controller and for the controlled plant are the same. This simulation is compared to the finite-state machine-based strategy performed in the real vehicle. The results show that NMPC keeps the battery at healthier temperatures and in addition reduces the cooling electrical consumption by more than 5%. In terms of the objective function, an accumulated and weighted sum of the two goals, this improvement amounts 30%. Finally, the online SIL presented in this paper, suggests that the used optimizer is fast enough for a future implementation in the vehicle.Accepted versio

The change towards a teaching methodology based on competences: a case study in a Spanish university

The European Higher Education Area (EHEA) has promoted the implementation of a teaching methodology based on competences. Drawing on New Institutional Sociology, the present work aims to identify and improve knowledge concerning the factors which are hindering that change in the Spanish university system. This is investigated using a case study based on a Spanish university which is a pioneer in the implementation of a competence-based curriculum. The results show that factors identified and analysed are conditioning the change and causing a ceremonial adoption of the competence-based system by the teaching staff. The results of the study may be of use as a reference and orientation for educators and administrators as well as for the regulators of those countries integrated within the EHEA. The study may prove useful in the analysis of inertia factors present when designing and implementing the policies and measures necessary to achieve a comprehensive teaching methodology; one which incorporates learning and assessment of competences in a real manner