Comparing Evolutionary Algorithms and Particle Filters for Markerless Human Motion Capture

Markerless Human Motion Capture is the problem of determining the joints’ angles of a three-dimensional articulated body model that best matches current and past observations acquired by video cameras. The problem of Markerless Human Motion Capture is high-dimensional and requires the use of models with a considerable number of degrees of freedom to appropriately adapt to the human anatomy. Particle filters have become the most popular approach for Markerless Human Motion Capture, despite their difficulty to cope with high-dimensional problems. Although several solutions have been proposed to improve their performance, they still suffer from the curse of dimensionality. As a consequence, it is normally required to impose mobility limitations in the body models employed, or to exploit the hierarchical nature of the human skeleton by partitioning the problem into smaller ones. Evolutionary algorithms, though, are powerful methods for solving continuous optimization problems, specially the high-dimensional ones. Yet, few works have tackled Markerless Human Motion Capture using them. This paper evaluates the performance of three of the most competitive algorithms in continuous optimization – Covariance Matrix Adaptation Evolutionary Strategy, Differential Evolution and Particle Swarm Optimization – with two of the most relevant particle filters proposed in the literature, namely the Annealed Particle Filter and the Partitioned Sampling Annealed Particle Filter. The algorithms have been experimentally compared in the public dataset HumanEva-I by employing two body models with different complexities. Our work also analyzes the performance of the algorithms in hierarchical and holistic approaches, i.e., with and without partitioning the search space. Non-parametric tests run on the results have shown that: (i) the evolutionary algorithms employed outperform their particle filter counterparts in all the cases tested; (ii) they can deal with high-dimensional models thus leading to better accuracy; and (iii) the hierarchical strategy surpasses the holistic one

Weighted Mean Curvature

In image processing tasks, spatial priors are essential for robust computations, regularization, algorithmic design and Bayesian inference. In this paper, we introduce weighted mean curvature (WMC) as a novel image prior and present an efficient computation scheme for its discretization in practical image processing applications. We first demonstrate the favorable properties of WMC, such as sampling invariance, scale invariance, and contrast invariance with Gaussian noise model; and we show the relation of WMC to area regularization. We further propose an efficient computation scheme for discretized WMC, which is demonstrated herein to process over 33.2 giga-pixels/second on GPU. This scheme yields itself to a convolutional neural network representation. Finally, WMC is evaluated on synthetic and real images, showing its superiority quantitatively to total-variation and mean curvature.Comment: 12 page

Parallelization Strategies for Markerless Human Motion Capture

Markerless Motion Capture (MMOCAP) is the problem of determining the pose of a person from images captured by one or several cameras simultaneously without using markers on the subject. Evaluation of the solutions is frequently the most time-consuming task, making most of the proposed methods inapplicable in real-time scenarios. This paper presents an efficient approach to parallelize the evaluation of the solutions in CPUs and GPUs. Our proposal is experimentally compared on six sequences of the HumanEva-I dataset using the CMAES algorithm. Multiple algorithm’s configurations were tested to analyze the best trade-off in regard to the accuracy and computing time. The proposed methods obtain speedups of 8× in multi-core CPUs, 30× in a single GPU and up to 110× using 4 GPU

Spectral imaging in preclinical research and clinical pathology.

Spectral imaging methods are attracting increased interest from researchers and practitioners in basic science, pre-clinical and clinical arenas. A combination of better labeling reagents and better optics creates opportunities to detect and measure multiple parameters at the molecular and cellular level. These tools can provide valuable insights into the basic mechanisms of life, and yield diagnostic and prognostic information for clinical applications. There are many multispectral technologies available, each with its own advantages and limitations. This chapter will present an overview of the rationale for spectral imaging, and discuss the hardware, software and sample labeling strategies that can optimize its usefulness in clinical settings

Defining Epidermal Basal Cell States during Skin Homeostasis and Wound Healing Using Single-Cell Transcriptomics.

Our knowledge of transcriptional heterogeneities in epithelial stem and progenitor cell compartments is limited. Epidermal basal cells sustain cutaneous tissue maintenance and drive wound healing. Previous studies have probed basal cell heterogeneity in stem and progenitor potential, but a comprehensive dissection of basal cell dynamics during differentiation is lacking. Using single-cell RNA sequencing coupled with RNAScope and fluorescence lifetime imaging, we identify three non-proliferative and one proliferative basal cell state in homeostatic skin that differ in metabolic preference and become spatially partitioned during wound re-epithelialization. Pseudotemporal trajectory and RNA velocity analyses predict a quasi-linear differentiation hierarchy where basal cells progress from Col17a1Hi/Trp63Hi state to early-response state, proliferate at the juncture of these two states, or become growth arrested before differentiating into spinous cells. Wound healing induces plasticity manifested by dynamic basal-spinous interconversions at multiple basal transcriptional states. Our study provides a systematic view of epidermal cellular dynamics, supporting a revised "hierarchical-lineage" model of homeostasis

Induction of fibroblast senescence generates a non-fibrogenic myofibroblast phenotype that differentially impacts on cancer prognosis

Cancer-associated fibroblasts (CAF) remain a poorly characterized, heterogeneous cell population. Here we characterized two previously described tumor-promoting CAF sub-types, smooth muscle actin (SMA)-positive myofibroblasts and senescent fibroblasts, identifying a novel link between the two