2,011 research outputs found
Detailed simulations of cell biology with Smoldyn 2.1.
Most cellular processes depend on intracellular locations and random collisions of individual protein molecules. To model these processes, we developed algorithms to simulate the diffusion, membrane interactions, and reactions of individual molecules, and implemented these in the Smoldyn program. Compared to the popular MCell and ChemCell simulators, we found that Smoldyn was in many cases more accurate, more computationally efficient, and easier to use. Using Smoldyn, we modeled pheromone response system signaling among yeast cells of opposite mating type. This model showed that secreted Bar1 protease might help a cell identify the fittest mating partner by sharpening the pheromone concentration gradient. This model involved about 200,000 protein molecules, about 7000 cubic microns of volume, and about 75 minutes of simulated time; it took about 10 hours to run. Over the next several years, as faster computers become available, Smoldyn will allow researchers to model and explore systems the size of entire bacterial and smaller eukaryotic cells
Multi-Scale Fluctuations in Non-Equilibrium Systems: Statistical Physics and Biological Application
Understanding how fluctuations continuously propagate across spatial scales is fundamental for our understanding of inanimate matter. This is exemplified by self-similar fluctuations in critical phenomena and the propagation of energy fluctuations described by the Kolmogorov-Law in turbulence. Our understanding is based on powerful theoretical frameworks that integrate fluctuations on intermediary scales, as in renormalisation group or coupled mode theory. In striking contrast to typical inanimate systems, living matter is typically organised into a hierarchy of processes on a discrete set of spatial scales: from biochemical processes embedded in dynamic subcellular compartments to cells giving rise to tissues. Therefore, the understanding of living matter requires novel theories that predict the interplay of fluctuations on multiple scales of biological organisation and the ensuing emergent degrees of freedom.
In this thesis, we derive a general theory of the multi-scale propagation of fluctuations in non-equilibrium systems and show that such processes underlie the regulation of cellular behaviour. Specifically, we draw on paradigmatic systems comprising stochastic many-particle systems undergoing dynamic compartmentalisation.
We first derive a theory for emergent degrees of freedom in open systems, where the total mass is not conserved. We show that the compartment dynamics give rise to the localisation of probability densities in phase space resembling quasi-particle behaviour. This emergent quasi-particle exhibits fundamentally different response kinetics and steady states compared to systems lacking compartment dynamics. In order to investigate a potential biological function of such quasi-particle dynamics, we then apply this theory to the regulation of cell death. We derive a model describing the subcellular processes that regulate cell death and show that the quasi-particle dynamics gives rise to a kinetic low-pass filter which suppresses the response of the cell to fast fluituations in cellular stress signals. We test our predictions experimentally by quantifying cell death in cell cultures subject to stress stimuli varying in strength and duration.
In closed systems, where the total mass is conserved, the effect of dynamic compartmentalisation depends on details of the kinetics on the scale of the stochastic many-particle dynamics. Using a second quantisation approach, we derive a commutator relation between the kinetic operators and the change in total entropy. Drawing on this, we show that the compartment dynamics alters the total entropy if the kinetics of the stochastic many-particle dynamics violate detailed balance. We apply this mechanism to the activation of cellular immune responses to RNA-virus infections. We show that dynamic compartmentalisation in closed systems gives rise to giant density fluctuations. This facilitates the emergence of gelation under conditions that violate theoretical gelation criteria in the absence of compartment dynamics. We show that such multi-scale gelation of protein complexes on the membranes of dynamic mitochondria governs the innate immune response.
Taken together, we provide a general theory describing the multi-scale propagation of fluctuations in biological systems. Our work pioneers the development of a statistical physics of such systems and highlights emergent degrees of freedom spanning different scales of biological organisation. By demonstrating that cells manipulate how fluctuations propagate across these scales, our work motivates a rethinking of how the behaviour of cells is regulated
The role of quantum information in thermodynamics --- a topical review
This topical review article gives an overview of the interplay between
quantum information theory and thermodynamics of quantum systems. We focus on
several trending topics including the foundations of statistical mechanics,
resource theories, entanglement in thermodynamic settings, fluctuation theorems
and thermal machines. This is not a comprehensive review of the diverse field
of quantum thermodynamics; rather, it is a convenient entry point for the
thermo-curious information theorist. Furthermore this review should facilitate
the unification and understanding of different interdisciplinary approaches
emerging in research groups around the world.Comment: published version. 34 pages, 6 figure
Foundations of Stochastic Thermodynamics
Small systems in a thermodynamic medium --- like colloids in a suspension or
the molecular machinery in living cells --- are strongly affected by the
thermal fluctuations of their environment. Physicists model such systems by
means of stochastic processes. Stochastic Thermodynamics (ST) defines entropy
changes and other thermodynamic notions for individual realizations of such
processes. It applies to situations far from equilibrium and provides a unified
approach to stochastic fluctuation relations. Its predictions have been studied
and verified experimentally.
This thesis addresses the theoretical foundations of ST. Its focus is on the
following two aspects: (i) The stochastic nature of mesoscopic observations has
its origin in the molecular chaos on the microscopic level. Can one derive ST
from an underlying reversible deterministic dynamics? Can we interpret ST's
notions of entropy and entropy changes in a well-defined
information-theoretical framework? (ii) Markovian jump processes on finite
state spaces are common models for bio-chemical pathways. How does one quantify
and calculate fluctuations of physical observables in such models? What role
does the topology of the network of states play? How can we apply our abstract
results to the design of models for molecular motors?
The thesis concludes with an outlook on dissipation as information written to
unobserved degrees of freedom --- a perspective that yields a consistency
criterion between dynamical models formulated on various levels of description.Comment: Ph.D. Thesis, G\"ottingen 2014, Keywords: Stochastic Thermodynamics,
Entropy, Dissipation, Markov processes, Large Deviation Theory, Molecular
Motors, Kinesi
The importance of thermodynamics for molecular systems, and the importance of molecular systems for thermodynamics
Improved understanding of molecular systems has only emphasised the sophistication of networks within the cell. Simultaneously, the advance of nucleic acid nanotechnology, a platform within which reactions can be exquisitely controlled, has made the development of artificial architectures and devices possible. Vital to this progress has been a solid foundation in the thermodynamics of molecular systems. In this pedagogical review and perspective, I will discuss how thermodynamics determines both the overall potential of molecular networks, and the minute details of design. I will then argue that, in turn, the need to understand molecular systems is helping to drive the development of theories of thermodynamics at the microscopic scale
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