Extracts of Feijoa Inhibit Toll-Like Receptor 2 Signaling and Activate Autophagy Implicating a Role in Dietary Control of IBD

Inflammatory bowel disease (IBD) is a heterogeneous chronic inflammatory disease affecting the gut with limited treatment success for its sufferers. This suggests the need for better understanding of the different subtypes of the disease as well as nutritional interventions to compliment current treatments. In this study we assess the ability of a hydrophilic feijoa fraction (F3) to modulate autophagy a process known to regulate inflammation, via TLR2 using IBD cell lines

Inhibition of inducible Nitric Oxide Synthase expression by an acetonic extract from Feijoa sellowiana Berg. fruits.

Feijoa sellowiana Berg. fruits and especially the acetonic extract have been shown to possess biological activities, although the responsible compounds have never been identified. The present study was designed to evaluate the anti-inflammatory activity of an acetonic extract from F. sellowiana Berg. fruits on the nitric oxide (NO) pathway, which plays an important role in inflammation. To this aim the J774 cell line, which expresses inducible nitric oxide synthase (iNOS) following stimulation with lipopolysaccharide (LPS), has been utilized, and the effects of this extract and its fractions on NO production, iNOS protein expression, and signal pathways involved in its regulation have been evaluated. This study demonstrates that at least some part of the anti-inflammatory activity of the acetonic extract is due to the suppression of NO production by flavone and stearic acid. The mechanism of this inhibition seems to be related to an action on the expression of the enzyme iNOS through the attenuation of nuclear factor κB (NF-κB) and/or mitogen-activated protein kinase (MAPK) activation

The establishment of Diachasmimorpha longicaudata (Hymenoptera: Braconidae) in Misiones, Northeastern Argentina

The establishment of Diachasmimorpha longicaudata (Ashmead) on Anastrepha fraterculus (Wiedemann) in the northeastern province of Misiones, Argentina, is described.Fil: Schliserman, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo. Centro de Investigaciones para la Regulación de Poblaciones de Organismos Nocivos; ArgentinaFil: Ovruski Alderete, Sergio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo. Centro de Investigaciones para la Regulación de Poblaciones de Organismos Nocivos; ArgentinaFil: De Coll, Olga R.. Instituto Nacional de Tecnología Agropecuaria; Argentin

Padrões de acúmulo de proteínas e carboidratos durante a embriogênese somática de Acca sellowiana

The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid-Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the somatic embryogenesis' in vitro morphogenesis and differentiation processes, the total protein levels decreased and the soluble sugars levels increased during the first 30 days in culture and remained stable until the 120th day. On the other hand, total protein levels increased according to the progression in the developmental stages of the somatic embryos. The levels of total sugars and starch increased in the heart and cotyledonary stages, and decreased in the torpedo and pre-cotyledonary stages. These compounds play a central role in the development of somatic embryos of Acca sellowiana. © 2009 Embrapa Informação Tecnológica.O objetivo deste trabalho foi quantificar os teores de proteína, amido e açúcares totais durante a histodiferenciação e desenvolvimento dos embriões somáticos em Acca sellowiana Berg. Para as observações histológicas, as amostras foram desidratadas em uma bateria de etanol, emblocadas em historesina e coradas com azul de toluidina (morfologia), azul de coomassie (corpos proteicos) e reativo ácido periódico de Schiff (amido). As proteínas foram extraídas usando uma solução tampão, precipitadas usando etanol e quantificadas por meio do reativo de Bradford. Os açúcares totais foram extraídos usando uma solução metanol-clorofórmioágua (12:5:3) e quantificados pela reação com antrona a 0,2%. O amido foi extraído usando uma solução de ácido perclórico a 30% e quantificado pela reação com antrona a 0,2%. Durante a diferenciação e morfogênese in vitro da embriogênese somática, os teores de proteínas totais decresceram e os açúcares solúveis aumentaram durante os 30 primeiros dias em cultura e permaneceram constantes até os 120 dias. Por outro lado, os teores das proteínas totais apresentaram incremento de acordo com a progressão nos estádios de desenvolvimento dos embriões somáticos. Os teores de açúcares totais e de amido aumentaram nos estádios cordiforme e cotiledonar e diminuíram nos estádios torpedo e pré-cotiledonar. Esses compostos exercem papel central no desenvolvimento de embriões somáticos de Acca sellowiana.Fil: Cangahuala-Inocente, Gabriela Claudia. Universidade Federal de Santa Catarina; BrasilFil: Steiner, Neusa. Universidade Federal de Santa Catarina; BrasilFil: Maldonado, Sara Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Guerra, Miguel Pedro. Universidade Federal de Santa Catarina; Brasi

Evaluación de la viabilidad para la producción y comercialización de bocadillo de feijoa

Trabajo de investigaciónEn el período comprendido entre los años 2013 y 2016, la producción del cultivo de feijoa presentó un aumento importante en la región de Cundinamarca, lo anterior según cifras del ministerio de agricultura (Agronet, 2016). Observando el aumento progresivo de este fruto en la región y las amplias posibilidades de transformar esta materia prima en productos terminados, el equipo de trabajo pretende determinar si es posible obtener o generar ingresos a través de la producción y comercialización de bocadillo derivado de la feijoa. Es importante tener presente que la comercialización de la feijoa se realiza en un período no mayor a 5 días (Estudio de Mercados Para la Feijoa (Acca sellowiana Berg) en fresco producida en el Municipio de Tibasosa (Boyacá Colombia) Página 272), teniendo en cuenta que pasado este tiempo el fruto empieza a perder sus características físicas (color y sabor) y por ende disminuye su calidad. Por lo anterior, se pretende que con la producción del bocadillo de feijoa (producto terminado) se puedan aprovechar las bondades nutricionales del fruto y extender la vida útil del producto. Considerando que el bocadillo es una de las golosinas más apetecidas por los consumidores” (Revista de Investigación, 176, 3) en el presente trabajo se realizarán los estudios correspondientes para determinar la factibilidad de producir bocadillo de feijoa en la finca de San Isidro ubicada en el municipio de Ubaque (Cundinamarca) y su comercialización en la ciudad de Bogotá.RESUMEN 1. MARCO TEÓRICO 2. OBJETIVOS 3. DISEÑO METODOLÓGICO 4. CÁLCULO DE LA DEMANDA 5. VIABILIDAD FINANCIERA 6. VALORACIÓN DE IMPACTOS AMBIENTALES 7. CONCLUSIONES 8. ASPECTOS FINALES BIBLIOGRAFÍA ANEXOSEspecializaciónEspecialista en Formulación y Evaluación Social y Económica de Proyecto

Cultivo e conservação da feijoa: Uma homenagem a um agricultor guardião.

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Controle do fungo Colletotrichum gloeosporioides em plantas de goiaba-serrana (Acca sellowiana) (in vitro e in vivo) com óleo essencial de Eucalypto viminalis

Projeto acadêmico (graduação) - Universidade Federal de Santa Catarina. Campus Curitibanos. Ciências Rurais.Por ser susceptível a diversas doenças, tais como a antracnose, a goiaba-serrana é uma planta que tem dificuldade em ter uma produção em quantidade e qualidade. Com isto, este trabalho vem pesquisar um tratamento alternativo para combater o fungo Colletotrichum gloeosporioides, o causador da antracnose, utilizando o óleo essencial da espécie Eucalypto viminalis. O Eucalypto viminalis é uma planta que tem um potencial enorme para combater fungos, pois é formado por grande quantidade de compostos antibacterianos e antifúngicos. Assim, a aplicação do óleo de eucalipto será empregado para combater o fungo, os testes serão realizando in vitro para combater um eventual problema que possa surgir, e obter um potencial elevado do seu óleo. Quando os resultados do laboratório forem analisados, e constatado que tem potencial, será feita a avaliação in vivo nas plantas. E assim propiciar aos produtores um maior interesse para produzir goiaba-serrana, não havendo grande porcentagem de doença, e otimizando o preço da fruta no mercado

Effect of food lures for monitoring of Anastrepha fraterculus (Diptera: Tephritidae) in Acca sellowiana (Myrtaceae).

The South American fruit fly, Anastrepha fraterculus (Diptera: Tephritidae) stands out for its polyphagous habit of damaging the production of several fruits in southern Brazil. This study aimed to evaluate the capture efficiency of A. fraterculus using grape juice at different periods of decomposition and aging as well as to test the capture efficiency of the enzymatic hydrolyzed protein Cera Trap® in feijoa crops. The work was conducted in a commercial feijoa orchard in São Joaquim, SC, Brazil during the 2014 growing season. Undiluted Cera Trap®, fresh grape juice and grape juice that had aged for 7 and 14 days were evaluated. All of the treatments with grape juice were used at the recommended concentration of 25 %. McPhail traps were used with 300 mL for each lure. On a weekly basis, the number of adults and the percentage of female fruit flies captured were evaluated, using identification and counting. Cera Trap® was the lure that captured the highest number of fruit flies, with a high percentage of females and with a higher frequency of captures during the season. Cera Trap® also showed the highest number of action thresholds compared to grape juice treatments. The 25 % fresh and aged grape juice showed a low number of captures and a low number of action thresholds. We conclude that fresh and aged grape juice were not effective for capturing and monitoring A. fraterculus in feijoa orchards. The Cera Trap® lure proves to be an alternative to improve monitoring of A. fraterculus in orchards in southern Brazil. Key words: Monitoring, fruit fly, control index, attractiveness, pest management. La mosca suramericana de las frutas Anastrepha fraterculus (Diptera: Tephritidae) se destaca por su hábito polífago, dañando la producción de diversas frutas en el sur de Brasil. El objetivo de este estudio fue evaluar la eficacia de la captura de moscas de la fruta utilizando jugo de uva en diferentes estados de descomposición y envejecimiento, así como evaluar la eficiencia de la captura de A. fraterculus con la proteína hidrolizada de origen animal Cera Trap® en un huerto de Acca sellowiana. El trabajo fue realizado en São Joaquim, SC, Brasil, durante la fructificación de 2014. Los tratamientos fueron: Cera Trap® sin diluir y jugo de uva fresco o envejecido durante 7 y 14 días. Los tratamientos con jugo de uva fueron utilizados a una concentración del 25 %. Se utilizaron trampas McPhail que contenían 300 mL de cada atrayente. Se evaluaron semanalmente el número de moscas adultas de las frutas y el porcentaje de hembras capturadas mediante el recuento e identificación de las especies. Cera Trap® fue el atrayente con el mayor número y mayor porcentaje de hembras de moscas capturadas, y de mayor frecuencia de capturas durante la estación proporcionando más posibilidades de control en comparación con los tratamientos que contenían jugo de uva. Se concluye que los jugos de uva fresca o envejecidos no fueron eficaces en la captura y monitoreo de A. fraterculus en huerto comercial de feijoa. El atrayente Cera Trap® demostró ser una alternativa para mejorar el monitoreo de A. fraterculus en huertos de feijoa. Palabras clave: Monitoreo, moscas de las frutas, instrucción de control, atracción, manejo de plagas

Feijoa Fruit Peel: Micro-morphological Features, Evaluation of Phytochemical Profile, and Biological Properties of Its Essential Oil

Acca sellowiana (O. Berg) Burret (Feijoa) is an evergreen shrub, belonging to the Mirtaceae family. The aim of this study was to investigate the micromorphological features of the feijoa fruit peel and to evaluate the phytochemical profile, as well as the antioxidant, cytoprotective, and antimicrobial properties of its essential oil (EO), by several in vitro cell-free and cell-based assays. The micromorphological analysis showed several schizogenic secretory cavities, immediately below the epidermal layer. Forty compounds were identified and quantified by GC-FID and GC-MS analyses. Sesquiterpenes were the most abundant ones (76.89%), followed by monoterpene hydrocarbons (3.26%), and oxygenated monoterpenes (0.34%). The main compounds were \u3b3-Selinene (17.39%), \u3b1-Cariophyllene (16.74%), \u3b2-Cariophyllene (10.37%), and Germacene D (5.32%). The EO showed a strong and dose-dependent antioxidant, and free-radical scavenging activity. Furthermore, it showed cytoprotective activity on the lymphocytes, that have been pre-treated with 100 \u3bcM tert-butyl-hydroperoxide (t-BOOH), as well as a decrease in intracellular reactive oxygen species (ROS), induced by t-BOOH on erythrocytes. A preliminary antimicrobial screening against GRAM+ and GRAM- bacteria, as well as on fungi highlighted that EO showed the best activity against S. aureus and C. albicans (MIC 2.7 mg/mL). In light of these results, feijoa fruit EO could find various applications, especially in the food, nutraceutical, and pharmaceutical fields