The microtubule cross-linker Feo controls the midzone stability, motor composition, and elongation of the anaphase B spindle in Drosophila embryos.

Chromosome segregation during anaphase depends on chromosome-to-pole motility and pole-to-pole separation. We propose that in Drosophila embryos, the latter process (anaphase B) depends on a persistent kinesin-5-generated interpolar (ip) microtubule (MT) sliding filament mechanism that "engages" to push apart the spindle poles when poleward flux is turned off. Here we investigated the contribution of the midzonal, antiparallel MT-cross-linking nonmotor MAP, Feo, to this "slide-and-flux-or-elongate" mechanism. Whereas Feo homologues in other systems enhance the midzone localization of the MT-MT cross-linking motors kinesin-4, -5 and -6, the midzone localization of these motors is respectively enhanced, reduced, and unaffected by Feo. Strikingly, kinesin-5 localizes all along ipMTs of the anaphase B spindle in the presence of Feo, including at the midzone, but the antibody-induced dissociation of Feo increases kinesin-5 association with the midzone, which becomes abnormally narrow, leading to impaired anaphase B and incomplete chromosome segregation. Thus, although Feo and kinesin-5 both preferentially cross-link MTs into antiparallel polarity patterns, kinesin-5 cannot substitute for loss of Feo function. We propose that Feo controls the organization, stability, and motor composition of antiparallel ipMTs at the midzone, thereby facilitating the kinesin-5-driven sliding filament mechanism underlying proper anaphase B spindle elongation and chromosome segregation

Fighting Irrelevance: The Role of Regional Trade Agreements in International Production Networks in Asia

This chapter contains the sectoral case study on the Thai hard disk drive (HDD) industry. The HDD industry in Thailand was first established during the early 1980s. By 2008, Thailand had become the second-largest HDD exporter in the world, accounting for about 17.4 per cent of world exports. Four out of six major HDD producers have affiliates in Thailand, including Seagate (since 1983), Hitachi GST (since 1991), Western Digital (since 2002) and Toshiba (since 2008). Thus, sufficient time has passed for an assessment to be made of the development of Thailand’s HDD industry.trade liberalization, international production networks, regional trade agreements, value chain, Asia, hard disk drive, East Asia, Thailand

A novel chromosome segregation mechanism during female meiosis.

In a wide range of eukaryotes, chromosome segregation occurs through anaphase A, in which chromosomes move toward stationary spindle poles, anaphase B, in which chromosomes move at the same velocity as outwardly moving spindle poles, or both. In contrast, Caenorhabditis elegans female meiotic spindles initially shorten in the pole-to-pole axis such that spindle poles contact the outer kinetochore before the start of anaphase chromosome separation. Once the spindle pole-to-kinetochore contact has been made, the homologues of a 4-μm-long bivalent begin to separate. The spindle shortens an additional 0.5 μm until the chromosomes are embedded in the spindle poles. Chromosomes then separate at the same velocity as the spindle poles in an anaphase B-like movement. We conclude that the majority of meiotic chromosome movement is caused by shortening of the spindle to bring poles in contact with the chromosomes, followed by separation of chromosome-bound poles by outward sliding

Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans.

Oocyte meiotic spindles orient with one pole juxtaposed to the cortex to facilitate extrusion of chromosomes into polar bodies. In Caenorhabditis elegans, these acentriolar spindles initially orient parallel to the cortex and then rotate to the perpendicular orientation. To understand the mechanism of spindle rotation, we characterized events that correlated temporally with rotation, including shortening of the spindle in the pole-to pole axis, which resulted in a nearly spherical spindle at rotation. By analyzing large spindles of polyploid C. elegans and a related nematode species, we found that spindle rotation initiated at a defined spherical shape rather than at a defined spindle length. In addition, dynein accumulated on the cortex just before rotation, and microtubules grew from the spindle with plus ends outward during rotation. Dynactin depletion prevented accumulation of dynein on the cortex and prevented spindle rotation independently of effects on spindle shape. These results support a cortical pulling model in which spindle shape might facilitate rotation because a sphere can rotate without deforming the adjacent elastic cytoplasm. We also present evidence that activation of spindle rotation is promoted by dephosphorylation of the basic domain of p150 dynactin

Planetary surface exploration: MESUR/autonomous lunar rover

Planetary surface exploration micro-rovers for collecting data about the Moon and Mars was designed by the Department of Mechanical Engineering at the University of Idaho. The goal of both projects was to design a rover concept that best satisfied the project objectives for NASA-Ames. A second goal was to facilitate student learning about the process of design. The first micro-rover is a deployment mechanism for the Mars Environmental SURvey (MESUR) Alpha Particle/Proton/X-ray instruments (APX). The system is to be launched with the sixteen MESUR landers around the turn of the century. A Tubular Deployment System and a spiked-legged walker was developed to deploy the APX from the lander to the Martian surface. While on Mars the walker is designed to take the APX to rocks to obtain elemental composition data of the surface. The second micro-rover is an autonomous, roving vehicle to transport a sensor package over the surface of the moon. The vehicle must negotiate the lunar-terrain for a minimum of one year by surviving impacts and withstanding the environmental extremes. The rover is a reliable track-driven unit that operates regardless of orientation which NASA can use for future lunar exploratory missions. A detailed description of the designs, methods, and procedures which the University of Idaho design teams followed to arrive at the final designs are included

Cost Basis of Grinding vs. Machining Machines

Grinding and machine tool machining are seperated by a gap in technological knowledge. This project investigated the gap between the two areas in a functional comparison. The two methods were found to be comparable with regards to hard materials and high precision. The difference in price was accounted for by production volume. Grinding may find a way to keep a competitive edge in the marketplace if a common ground for function and performance provide for cheaper parts in the industry

Possible origins of macroscopic left-right asymmetry in organisms

I consider the microscopic mechanisms by which a particular left-right (L/R) asymmetry is generated at the organism level from the microscopic handedness of cytoskeletal molecules. In light of a fundamental symmetry principle, the typical pattern-formation mechanisms of diffusion plus regulation cannot implement the "right-hand rule"; at the microscopic level, the cell's cytoskeleton of chiral filaments seems always to be involved, usually in collective states driven by polymerization forces or molecular motors. It seems particularly easy for handedness to emerge in a shear or rotation in the background of an effectively two-dimensional system, such as the cell membrane or a layer of cells, as this requires no pre-existing axis apart from the layer normal. I detail a scenario involving actin/myosin layers in snails and in C. elegans, and also one about the microtubule layer in plant cells. I also survey the other examples that I am aware of, such as the emergence of handedness such as the emergence of handedness in neurons, in eukaryote cell motility, and in non-flagellated bacteria.Comment: 42 pages, 6 figures, resubmitted to J. Stat. Phys. special issue. Major rewrite, rearranged sections/subsections, new Fig 3 + 6, new physics in Sec 2.4 and 3.4.1, added Sec 5 and subsections of Sec

Design of a Helicopter Hover Test Stand

The goal of this project is to design, build, and test a fully articulated helicopter rotor head system for future implementation on a hover test stand. The stand will be used to measure the forces and moments at the blade roots and the strain along the blades. The design of the rotor head is modular, allowing for the type and number of blades to be changed as desired without major disassembly of the test stand. The design is based on a fully articulated, four bladed rotor head with a custom fabricated swashplate and driveshaft. Additionally, a safety system was designed to ensure the safe operation of the hover test stand and protect the users in the case of failure at maximum rotor speed

Tribological Studies on Scuffing Due to the Influence of Carbon Dioxide Used as a Refrigerant in Compressors

The refrigeration and air conditioning industry has expressed a great interest in the use of carbon dioxide (CO2) as a refrigerant. CO2 is anticipated to replace HFC refrigerants, which are known to have a negative effect on the environment. The reason behind the interest in CO2 is the fact that it is a natural refrigerant, thus environmentally acceptable. Of course, such a replacement raises concerns regarding design criteria and performance due to the different thermodynamic properties of CO2 and the very different range of pressures required for the CO2 refrigeration cycle. So far, work related to CO2 has been done from a thermodynamics point of view and researchers have made significant progress developing automotive and portable air-conditioning systems that use the environmentally friendly carbon dioxide as a refrigerant. The purpose of this work is to develop an understanding of how CO2 plays a role from a tribology standpoint. More specifically, the goal of this work is to gain an understanding on how CO2 influences friction, lubrication, wear and scuffing of tribological pairs used in compressors. Work in the area of tribology related to CO2 is very limited. Preliminary work by Cusano and coworkers showed that consistent data for tests using CO2 could not be acquired nor could a satisfactory explanation be offered for the inconsistency. Their results triggered the initiation of the work presented here. In this first attempt to understand the tribological behavior of CO2 several problems were encountered. During this work we noted that its behavior, unlike conventional refrigerants, could not always be predicted. We believe that this can be attributed to the thermodynamic properties of CO2, which cannot be ignored when studying its tribological behavior. Thermodynamic Properties such as miscibility are very important when tribological testing is performed. A limiting factor with our tester was that it was not designed for CO2 testing, but for other conventional refrigerants and therefore made previously developed testing protocols non-applicable with CO2. Through a different approach and some modifications to our tester we were able to establish a protocol for testing under the presence of CO2. CO2 was then compared to R134a and the experimental results showed that it performs equally well.Air Conditioning and Refrigeration Project 13